GREMLIN Database
DUF2238 - Predicted membrane protein (DUF2238)
PFAM: PF09997 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 140 (138)
Sequences: 8954 (5297)
Seq/√Len: 450.9
META: 0.916

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
95_C99_A2.9061.00
30_L66_A2.6421.00
46_F49_Q2.5711.00
111_A115_L2.4921.00
67_Q100_I2.2741.00
91_F95_C2.1551.00
54_L58_H2.0371.00
90_F94_V2.0361.00
45_G48_L2.0341.00
33_V42_V2.0331.00
98_L102_A2.0231.00
102_A106_L1.9621.00
20_S73_I1.9521.00
110_W114_A1.9201.00
61_R135_K1.8411.00
48_L62_L1.7981.00
58_H61_R1.7811.00
72_A93_V1.7721.00
29_L33_V1.7431.00
18_P22_T1.6881.00
89_L93_V1.6611.00
5_P9_A1.6361.00
135_K138_A1.6321.00
92_L96_V1.6131.00
103_L110_W1.5671.00
66_A70_V1.5591.00
17_T77_E1.5291.00
80_L89_L1.5191.00
115_L118_E1.4821.00
46_F50_D1.4791.00
75_A96_V1.4391.00
28_A32_M1.4321.00
106_L110_W1.3881.00
129_D135_K1.3861.00
115_L119_A1.3781.00
32_M35_G1.3611.00
118_E121_D1.3601.00
8_V11_Y1.3551.00
41_E122_A1.3521.00
3_G74_P1.3441.00
109_W113_L1.3281.00
90_F93_V1.3271.00
20_S74_P1.3211.00
104_Y123_F1.2911.00
11_Y21_Y1.2571.00
49_Q54_L1.2551.00
135_K139_L1.2521.00
16_L24_I1.2321.00
76_R80_L1.2271.00
110_W113_L1.2251.00
41_E46_F1.2221.00
103_L106_L1.2171.00
47_W51_L1.2111.00
93_V97_C1.2071.00
30_L63_G1.2001.00
121_D126_T1.1981.00
23_L70_V1.1921.00
96_V100_I1.1771.00
34_G59_Y1.1751.00
61_R129_D1.1741.00
121_D130_V1.1701.00
10_T14_F1.1561.00
25_F32_M1.1561.00
42_V45_G1.1361.00
29_L32_M1.1331.00
119_A122_A1.1311.00
10_T21_Y1.1291.00
86_R90_F1.0661.00
22_T26_L1.0631.00
93_V99_A1.0621.00
115_L121_D1.0621.00
72_A97_C1.0551.00
38_T41_E1.0511.00
99_A102_A1.0501.00
55_S58_H1.0401.00
48_L54_L1.0351.00
112_A120_A1.0271.00
86_R89_L1.0271.00
109_W138_A1.0161.00
26_L66_A1.0101.00
11_Y15_P1.0051.00
94_V98_L1.0021.00
15_P18_P0.9921.00
12_R15_P0.9761.00
80_L83_A0.9681.00
9_A13_R0.9671.00
44_L48_L0.9591.00
87_G90_F0.9471.00
45_G62_L0.9401.00
96_V99_A0.9381.00
48_L52_F0.9331.00
106_L138_A0.9321.00
102_A137_M0.9291.00
24_I28_A0.9241.00
16_L20_S0.9131.00
110_W138_A0.9101.00
33_V44_L0.9081.00
116_G119_A0.9071.00
7_L10_T0.8991.00
92_L95_C0.8911.00
5_P8_V0.8901.00
67_Q101_S0.8821.00
79_L96_V0.8791.00
75_A93_V0.8681.00
22_T25_F0.8541.00
131_W134_Q0.8521.00
79_L85_L0.8461.00
9_A12_R0.8461.00
7_L21_Y0.8451.00
27_H31_L0.8401.00
45_G58_H0.8371.00
23_L73_I0.8341.00
48_L51_L0.8141.00
4_L8_V0.8101.00
63_G67_Q0.8011.00
79_L92_L0.7961.00
61_R139_L0.7941.00
98_L103_L0.7871.00
10_T13_R0.7761.00
83_A87_G0.7751.00
47_W50_D0.7741.00
107_I111_A0.7721.00
84_P88_W0.7671.00
24_I74_P0.7671.00
51_L54_L0.7611.00
19_L23_L0.7611.00
20_S77_E0.7591.00
14_F82_K0.7561.00
20_S27_H0.7541.00
33_V37_Y0.7501.00
6_L10_T0.7491.00
45_G51_L0.7481.00
24_I71_P0.7421.00
72_A76_R0.7371.00
83_A88_W0.7351.00
94_V106_L0.7341.00
84_P89_L0.7311.00
10_T16_L0.7251.00
113_L135_K0.7231.00
109_W135_K0.7151.00
65_F139_L0.7151.00
44_L62_L0.7131.00
126_T133_T0.7121.00
45_G50_D0.7101.00
58_H139_L0.7091.00
79_L83_A0.6891.00
2_I5_P0.6881.00
78_I82_K0.6851.00
20_S23_L0.6831.00
66_A69_F0.6801.00
79_L89_L0.6761.00
46_F62_L0.6711.00
33_V36_H0.6561.00
106_L134_Q0.6561.00
88_W91_F0.6300.99
75_A97_C0.6270.99
107_I123_F0.6250.99
118_E122_A0.6220.99
25_F28_A0.6170.99
83_A86_R0.6120.99
126_T130_V0.6120.99
51_L55_S0.5950.99
125_G133_T0.5930.99
95_C103_L0.5930.99
44_L51_L0.5880.99
36_H41_E0.5850.99
63_G66_A0.5840.99
85_L89_L0.5840.99
7_L24_I0.5830.99
112_A116_G0.5830.99
16_L21_Y0.5780.99
25_F29_L0.5760.99
91_F100_I0.5740.99
46_F51_L0.5710.99
100_I103_L0.5710.99
22_T29_L0.5700.99
49_Q55_S0.5680.99
79_L84_P0.5630.99
75_A95_C0.5550.99
11_Y14_F0.5540.99
53_G56_R0.5540.99
52_F56_R0.5520.98
17_T20_S0.5510.98
70_V74_P0.5470.98
122_A125_G0.5410.98
101_S137_M0.5410.98
58_H62_L0.5400.98
68_G71_P0.5390.98
44_L49_Q0.5390.98
126_T134_Q0.5370.98
104_Y125_G0.5330.98
45_G49_Q0.5320.98
14_F81_R0.5320.98
91_F96_V0.5310.98
28_A31_L0.5260.98
120_A124_L0.5240.98
88_W93_V0.5230.98
122_A126_T0.5170.98
112_A126_T0.5130.98
52_F58_H0.5130.98
11_Y18_P0.5120.98
67_Q71_P0.5100.97
70_V73_I0.5050.97
98_L137_M0.5030.97
19_L73_I0.5020.97
37_Y43_P0.5020.97
71_P74_P0.5000.97
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2ls2A 1 0.1786 3.8 0.945 Contact Map
4njnA 4 0.45 2.3 0.951 Contact Map
2nr9A 2 0.5143 2.1 0.952 Contact Map
3tj6B 1 0.0929 1.8 0.953 Contact Map
4pd6A 3 0.5357 1.6 0.955 Contact Map
2cvcA 1 0.0643 1.5 0.955 Contact Map
2o9gA 3 0.6 1.4 0.956 Contact Map
2a40C 1 0.0714 1.4 0.957 Contact Map
3ze3A 4 0.5857 1.3 0.957 Contact Map
3p5nA 2 0.5286 1.1 0.959 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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