GREMLIN Database
DUF2236 - Uncharacterized protein conserved in bacteria (DUF2236)
PFAM: PF09995 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 218 (206)
Sequences: 14909 (9613)
Seq/√Len: 669.8
META: 0.749

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
115_S118_E3.8311.00
143_T146_E3.7431.00
109_E119_R3.6021.00
58_T61_E2.8701.00
39_D42_G2.7961.00
119_R144_R2.6981.00
199_S202_R2.6431.00
161_V166_R2.5811.00
16_R102_D2.5601.00
91_E158_E2.4221.00
144_R148_R2.4101.00
121_A125_E2.4001.00
136_P139_D2.3781.00
74_A88_I2.2671.00
63_E67_A2.0661.00
131_R137_A2.0621.00
120_D124_A2.0411.00
187_W191_L1.9881.00
209_L213_A1.9611.00
53_T65_A1.9551.00
149_A153_A1.9231.00
59_R63_E1.9091.00
80_L83_R1.9031.00
127_R142_A1.8781.00
77_R86_S1.8731.00
94_L150_Y1.8341.00
29_A33_E1.8111.00
70_R88_I1.8001.00
16_R103_S1.7971.00
162_T165_A1.7941.00
124_A127_R1.7681.00
105_L119_R1.7671.00
128_R131_R1.7451.00
102_D106_R1.7351.00
198_W202_R1.7291.00
50_F69_V1.6891.00
63_E132_L1.6231.00
128_R132_L1.6051.00
71_R74_A1.5921.00
98_A147_F1.5891.00
163_P167_E1.5791.00
42_G46_R1.5561.00
156_R159_L1.5541.00
116_P119_R1.5511.00
142_A146_E1.5461.00
153_A157_P1.5161.00
203_E210_R1.4981.00
50_F65_A1.4731.00
98_A150_Y1.4711.00
105_L144_R1.4631.00
97_H130_G1.4191.00
156_R187_W1.3991.00
181_V194_L1.3951.00
53_T61_E1.3931.00
101_V126_W1.3481.00
116_P144_R1.3361.00
123_Y147_F1.3271.00
150_Y154_M1.3261.00
116_P120_D1.3161.00
202_R205_A1.3111.00
130_G140_L1.3021.00
148_R152_D1.2981.00
164_A167_E1.2971.00
120_D144_R1.2941.00
198_W201_R1.2841.00
131_R142_A1.2641.00
145_A149_A1.2591.00
186_P190_E1.2541.00
116_P145_A1.2521.00
90_P136_P1.2441.00
117_A121_A1.2321.00
106_R109_E1.2261.00
200_P204_R1.2241.00
102_D148_R1.2061.00
161_V165_A1.1891.00
72_I75_R1.1891.00
26_L92_L1.1811.00
70_R134_G1.1691.00
118_E121_A1.1661.00
151_L155_L1.1591.00
188_L191_L1.1331.00
29_A164_A1.1071.00
163_P166_R1.0971.00
181_V189_R1.0711.00
198_W203_E1.0691.00
210_R213_A1.0601.00
201_R205_A1.0591.00
182_G198_W1.0501.00
24_H28_A1.0501.00
16_R106_R1.0471.00
8_A12_L1.0461.00
127_R140_L1.0431.00
108_Y112_R1.0431.00
199_S203_E1.0361.00
154_M158_E1.0351.00
8_A11_L1.0351.00
106_R110_R1.0351.00
26_L80_L1.0341.00
165_A168_L1.0321.00
107_A110_R1.0261.00
30_G35_S1.0241.00
109_E113_P1.0211.00
94_L139_D1.0191.00
161_V191_L1.0061.00
120_D145_A1.0061.00
198_W204_R0.9921.00
32_A164_A0.9721.00
105_L122_Y0.9701.00
121_A124_A0.9671.00
114_L118_E0.9541.00
26_L30_G0.9481.00
65_A68_R0.9401.00
66_A132_L0.9391.00
200_P205_A0.9311.00
60_E63_E0.9241.00
89_D92_L0.9201.00
107_A111_F0.9151.00
77_R88_I0.9081.00
36_D39_D0.9031.00
9_A12_L0.8921.00
171_P174_R0.8861.00
50_F66_A0.8801.00
26_L29_A0.8801.00
30_G34_H0.8771.00
20_L188_L0.8761.00
64_A68_R0.8761.00
26_L89_D0.8751.00
44_L48_A0.8741.00
93_L135_V0.8711.00
126_W129_V0.8701.00
27_V30_G0.8671.00
130_G135_V0.8631.00
124_A142_A0.8611.00
32_A35_S0.8591.00
15_L19_L0.8581.00
127_R131_R0.8561.00
185_P188_L0.8541.00
205_A211_A0.8501.00
135_V140_L0.8401.00
174_R177_R0.8381.00
103_S106_R0.8371.00
94_L140_L0.8341.00
120_D123_Y0.8331.00
184_L188_L0.8311.00
24_H154_M0.8311.00
3_V6_D0.8291.00
152_D156_R0.8291.00
71_R75_R0.8251.00
200_P203_E0.8241.00
67_A71_R0.8221.00
48_A52_A0.8141.00
67_A70_R0.8111.00
14_G18_L0.8111.00
76_V87_A0.8091.00
16_R19_L0.8081.00
189_R196_R0.8081.00
98_A151_L0.8081.00
181_V190_E0.8071.00
201_R204_R0.8011.00
177_R180_A0.8011.00
59_R62_A0.7991.00
53_T68_R0.7941.00
19_L22_A0.7921.00
102_D151_L0.7881.00
68_R71_R0.7861.00
49_R53_T0.7861.00
26_L83_R0.7821.00
47_T96_V0.7801.00
109_E112_R0.7801.00
123_Y144_R0.7761.00
4_H122_Y0.7711.00
27_V96_V0.7671.00
61_E64_A0.7621.00
23_A28_A0.7571.00
34_H85_Y0.7571.00
173_Y176_L0.7571.00
177_R181_V0.7571.00
185_P189_R0.7551.00
18_L21_Q0.7491.00
22_A168_L0.7461.00
105_L109_E0.7411.00
160_R165_A0.7391.00
68_R72_I0.7371.00
31_V35_S0.7331.00
111_F179_A0.7271.00
50_F68_R0.7261.00
186_P196_R0.7251.00
50_F133_L0.7251.00
28_A31_V0.7241.00
114_L119_R0.7211.00
106_R148_R0.7191.00
135_V139_D0.7181.00
51_V100_L0.7171.00
173_Y177_R0.7151.00
51_V54_T0.7151.00
60_E64_A0.7141.00
57_G61_E0.7111.00
176_L180_A0.7041.00
31_V34_H0.7021.00
176_L179_A0.7001.00
149_A152_D0.7001.00
28_A168_L0.6951.00
127_R137_A0.6911.00
22_A31_V0.6901.00
62_A132_L0.6881.00
209_L212_L0.6851.00
147_F151_L0.6851.00
184_L192_L0.6841.00
116_P148_R0.6801.00
205_A209_L0.6781.00
174_R178_A0.6781.00
187_W190_E0.6771.00
25_P28_A0.6761.00
160_R191_L0.6741.00
151_L154_M0.6741.00
146_E149_A0.6721.00
45_R49_R0.6721.00
33_E164_A0.6691.00
180_A184_L0.6681.00
190_E196_R0.6661.00
166_R193_G0.6661.00
24_H27_V0.6651.00
152_D155_L0.6631.00
23_A188_L0.6611.00
119_R148_R0.6611.00
29_A162_T0.6571.00
108_Y114_L0.6561.00
153_A158_E0.6551.00
54_T69_V0.6481.00
100_L126_W0.6471.00
137_A142_A0.6441.00
70_R74_A0.6431.00
101_V123_Y0.6421.00
25_P31_V0.6411.00
91_E139_D0.6401.00
91_E94_L0.6381.00
145_A148_R0.6321.00
199_S205_A0.6311.00
103_S107_A0.6241.00
150_Y153_A0.6211.00
155_L159_L0.6171.00
147_F150_Y0.6171.00
164_A168_L0.6131.00
14_G19_L0.6121.00
198_W205_A0.6111.00
69_V133_L0.6081.00
63_E70_R0.6081.00
178_A202_R0.6081.00
110_R183_L0.6071.00
54_T129_V0.6061.00
109_E144_R0.6001.00
178_A182_G0.6001.00
12_L16_R0.6001.00
16_R183_L0.5991.00
67_A132_L0.5971.00
23_A27_V0.5971.00
203_E209_L0.5971.00
13_G17_A0.5951.00
6_D10_L0.5941.00
189_R198_W0.5921.00
24_H29_A0.5901.00
66_A133_L0.5841.00
28_A165_A0.5831.00
108_Y111_F0.5821.00
123_Y142_A0.5811.00
59_R132_L0.5811.00
30_G76_V0.5801.00
94_L138_E0.5781.00
146_E150_Y0.5781.00
78_G85_Y0.5741.00
26_L91_E0.5741.00
64_A71_R0.5671.00
78_G84_P0.5621.00
95_W99_T0.5591.00
74_A86_S0.5551.00
28_A32_A0.5541.00
18_L22_A0.5531.00
189_R194_L0.5511.00
43_R47_T0.5511.00
6_D9_A0.5441.00
199_S204_R0.5421.00
175_L179_A0.5421.00
26_L31_V0.5401.00
86_S89_D0.5391.00
14_G21_Q0.5371.00
172_L176_L0.5361.00
4_H10_L0.5341.00
15_L180_A0.5311.00
155_L158_E0.5311.00
13_G103_S0.5291.00
79_T84_P0.5281.00
91_E154_M0.5271.00
41_W45_R0.5260.99
27_V31_V0.5220.99
165_A169_A0.5210.99
160_R164_A0.5210.99
97_H123_Y0.5170.99
101_V105_L0.5160.99
106_R151_L0.5150.99
204_R211_A0.5090.99
123_Y127_R0.5080.99
55_T125_E0.5080.99
178_A205_A0.5060.99
5_G8_A0.5050.99
17_A20_L0.5040.99
66_A70_R0.5030.99
88_I93_L0.5010.99
198_W209_L0.5010.99
180_A183_L0.5000.99
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3ubcA 3 0.5459 15.4 0.939 Contact Map
1tu9A 1 0.5596 12.8 0.941 Contact Map
3g46A 2 0.5321 12.1 0.942 Contact Map
4hrrA 1 0.5459 8.4 0.946 Contact Map
3wfwA 2 0.578 8.2 0.946 Contact Map
1hlbA 1 0.5642 8.1 0.946 Contact Map
4hrrB 2 0.5459 7.6 0.947 Contact Map
1binA 1 0.555 6.5 0.948 Contact Map
1hlmA 2 0.5688 6 0.949 Contact Map
3vfzA 2 0.2615 5.2 0.951 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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