GREMLIN Database
DUF2231 - Predicted membrane protein (DUF2231)
PFAM: PF09990 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 104 (98)
Sequences: 19344 (12459)
Seq/√Len: 1258.6
META: 0.953

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
46_L90_T3.4851.00
47_N90_T2.7721.00
54_F83_G2.4811.00
96_E100_R2.3081.00
27_W41_L2.1111.00
10_A62_L1.9821.00
16_A51_L1.9381.00
53_L83_G1.8971.00
61_S64_L1.8931.00
50_A90_T1.8101.00
42_L45_L1.7131.00
38_R42_L1.6531.00
48_V52_V1.5841.00
20_A51_L1.5611.00
50_A87_L1.5461.00
27_W40_A1.4701.00
95_G99_Y1.4631.00
61_S80_S1.4591.00
73_P76_G1.4411.00
61_S65_R1.4101.00
45_L49_V1.3971.00
39_I93_L1.3951.00
6_Y66_R1.3711.00
42_L93_L1.3691.00
25_I29_G1.3671.00
14_I18_L1.3481.00
41_L45_L1.3091.00
32_P37_K1.2721.00
66_R69_P1.2661.00
47_N50_A1.2631.00
62_L66_R1.2601.00
17_L55_A1.2541.00
6_Y10_A1.2521.00
24_L28_L1.2511.00
12_G54_F1.2471.00
62_L65_R1.2331.00
15_G18_L1.2311.00
72_V76_G1.2311.00
48_V51_L1.2301.00
50_A86_L1.2231.00
76_G80_S1.2211.00
13_L55_A1.2091.00
15_G19_A1.2031.00
24_L44_A1.1851.00
41_L44_A1.1831.00
75_L78_A1.1711.00
91_G95_G1.1691.00
44_A48_V1.1391.00
39_I101_H1.1221.00
89_V93_L1.1201.00
2_A5_A1.1141.00
77_L81_L1.0801.00
4_A8_L1.0781.00
63_L67_R1.0611.00
4_A7_W1.0591.00
31_P34_T1.0401.00
27_W37_K1.0401.00
23_G43_H1.0241.00
46_L86_L1.0211.00
74_P78_A1.0071.00
63_L66_R1.0061.00
11_A15_G0.9941.00
85_A89_V0.9881.00
22_T25_I0.9871.00
52_V56_V0.9851.00
46_L50_A0.9701.00
64_L67_R0.9601.00
36_A103_V0.9581.00
78_A82_V0.9411.00
13_L17_L0.9401.00
84_V88_L0.9371.00
30_I34_T0.9321.00
64_L68_D0.9311.00
18_L22_T0.9121.00
55_A63_L0.8861.00
46_L89_V0.8771.00
26_D40_A0.8281.00
89_V92_W0.8241.00
5_A9_L0.8191.00
86_L90_T0.8111.00
96_E101_H0.8051.00
46_L93_L0.7941.00
81_L85_A0.7751.00
64_L76_G0.7721.00
13_L62_L0.7651.00
27_W45_L0.7651.00
13_L54_F0.7541.00
24_L48_V0.7471.00
84_V87_L0.7441.00
42_L89_V0.7441.00
65_R68_D0.7421.00
39_I97_L0.7201.00
56_V64_L0.7071.00
78_A81_L0.7051.00
20_A48_V0.7041.00
65_R69_P0.7041.00
8_L11_A0.7001.00
16_A19_A0.6971.00
30_I103_V0.6931.00
53_L86_L0.6901.00
61_S66_R0.6891.00
21_V25_I0.6881.00
40_A44_A0.6831.00
50_A54_F0.6781.00
50_A83_G0.6781.00
66_R70_A0.6751.00
92_W96_E0.6701.00
36_A101_H0.6681.00
27_W44_A0.6621.00
30_I36_A0.6611.00
26_D29_G0.6581.00
63_L68_D0.6501.00
74_P77_L0.6441.00
67_R70_A0.6431.00
99_Y102_G0.6341.00
42_L46_L0.6331.00
88_L92_W0.6131.00
35_R101_H0.6081.00
24_L27_W0.6071.00
30_I33_G0.5871.00
13_L16_A0.5841.00
22_T26_D0.5761.00
35_R38_R0.5761.00
51_L55_A0.5711.00
80_S84_V0.5701.00
32_P36_A0.5681.00
3_R8_L0.5661.00
17_L51_L0.5631.00
2_A7_W0.5621.00
5_A8_L0.5601.00
71_A76_G0.5581.00
92_W95_G0.5521.00
64_L69_P0.5511.00
64_L70_A0.5511.00
3_R6_Y0.5491.00
75_L79_L0.5471.00
39_I96_E0.5421.00
14_I17_L0.5411.00
25_I28_L0.5381.00
61_S68_D0.5361.00
13_L51_L0.5261.00
18_L21_V0.5231.00
47_N51_L0.5181.00
49_V86_L0.5181.00
49_V53_L0.5181.00
37_K40_A0.5031.00
61_S67_R0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4o6yA 2 0.9615 39.7 0.836 Contact Map
4i0uA 4 0.6058 23.2 0.854 Contact Map
4ev6A 3 0.625 18.9 0.86 Contact Map
3ayfA 2 0.9904 17.1 0.863 Contact Map
1kqfC 3 0.9615 7.5 0.883 Contact Map
4gd3A 2 0.9327 6.7 0.886 Contact Map
1rz2A 1 0.0769 6 0.888 Contact Map
3mk7A 1 0.9327 5.2 0.891 Contact Map
2lomA 1 0.6827 3.8 0.899 Contact Map
4pgrA 1 0.625 3 0.904 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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