GREMLIN Database
DUF2227 - Uncharacterized metal-binding protein (DUF2227)
PFAM: PF09988 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 169 (167)
Sequences: 1049 (873)
Seq/√Len: 67.6
META: 0.779

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
9_I158_A5.1911.00
10_T42_G4.9161.00
83_P153_A3.2931.00
37_G144_L3.0691.00
46_S55_Q3.0161.00
40_F44_M2.7141.00
87_T148_L2.6171.00
56_Y63_R2.4131.00
51_L70_Q2.3051.00
56_Y70_Q2.1761.00
7_D10_T2.1401.00
73_F89_L2.1271.00
77_S89_L2.1191.00
149_E152_A2.0961.00
7_D58_R2.0411.00
87_T152_A1.9011.00
155_H159_D1.8991.00
1_P5_T1.8481.00
53_S58_R1.8461.00
33_I37_G1.8261.00
87_T149_E1.7851.00
53_S59_W1.7691.00
1_P9_I1.7401.00
163_S166_K1.7401.00
156_T160_G1.7341.00
46_S58_R1.6520.99
45_F94_L1.6080.99
83_P87_T1.5970.99
53_S63_R1.5800.99
11_L15_P1.5560.99
30_L143_A1.5180.99
42_G151_G1.5150.99
137_Y140_E1.5000.99
82_G152_A1.4950.99
10_T151_G1.4940.99
45_F55_Q1.4880.99
59_W66_W1.4840.99
5_T9_I1.4580.98
52_Y70_Q1.4570.98
162_G166_K1.4520.98
45_F151_G1.4410.98
44_M55_Q1.4350.98
77_S82_G1.4200.98
115_G118_D1.4120.98
1_P158_A1.4050.98
53_S56_Y1.3790.98
131_L135_Q1.3500.97
21_A34_F1.3460.97
117_G121_A1.3420.97
102_L106_A1.3320.97
82_G85_I1.3160.97
41_G151_G1.3130.97
153_A156_T1.2930.97
109_L112_I1.2790.96
7_D45_F1.2670.96
127_L131_L1.2490.96
116_W123_L1.2380.96
12_L16_P1.2350.96
10_T154_S1.2240.95
13_L16_P1.2220.95
69_Y90_R1.2200.95
92_L95_A1.2030.95
4_R8_R1.2020.95
117_G120_L1.2000.95
38_F150_L1.1970.95
117_G123_L1.1890.95
10_T14_L1.1660.94
111_A114_G1.1550.94
72_L78_R1.1520.94
90_R93_Y1.1440.93
119_L122_S1.1380.93
78_R85_I1.1260.93
10_T152_A1.1160.93
53_S66_W1.1060.92
110_A114_G1.0990.92
41_G45_F1.0710.91
109_L113_G1.0470.90
10_T43_L1.0310.89
53_S68_P1.0300.89
47_P80_S1.0230.89
37_G72_L1.0230.89
34_F144_L1.0150.89
151_G156_T1.0120.88
59_W62_L0.9970.88
120_L123_L0.9920.87
162_G165_Y0.9920.87
47_P156_T0.9780.87
55_Q86_G0.9730.86
21_A31_T0.9660.86
136_L139_E0.9630.86
41_G148_L0.9570.85
18_A21_A0.9520.85
53_S70_Q0.9510.85
7_D42_G0.9470.85
33_I144_L0.9360.84
49_L66_W0.9280.84
68_P93_Y0.9260.84
45_F53_S0.9190.83
65_L68_P0.9190.83
77_S152_A0.9180.83
73_F78_R0.9110.83
4_R18_A0.9070.82
7_D82_G0.9050.82
140_E144_L0.8970.82
42_G149_E0.8960.81
37_G93_Y0.8890.81
56_Y67_L0.8880.81
10_T77_S0.8840.81
38_F149_E0.8780.80
118_D121_A0.8630.79
69_Y80_S0.8610.79
80_S90_R0.8600.79
34_F38_F0.8560.78
7_D54_R0.8450.78
46_S154_S0.8430.77
55_Q63_R0.8330.77
82_G156_T0.8310.76
83_P148_L0.8310.76
56_Y77_S0.8300.76
73_F80_S0.8300.76
3_G8_R0.8270.76
42_G152_A0.8200.75
52_Y71_K0.8110.75
58_R65_L0.8050.74
148_L152_A0.8050.74
41_G51_L0.8040.74
128_G131_L0.8030.74
115_G122_S0.8010.74
14_L18_A0.7880.72
19_A23_A0.7850.72
73_F77_S0.7700.71
7_D53_S0.7690.71
54_R57_R0.7680.70
41_G46_S0.7550.69
67_L71_K0.7550.69
54_R87_T0.7530.69
115_G120_L0.7520.69
47_P152_A0.7520.69
8_R12_L0.7480.68
138_P142_L0.7480.68
65_L97_L0.7410.68
15_P18_A0.7400.68
7_D70_Q0.7400.68
83_P152_A0.7390.68
123_L126_L0.7280.66
38_F47_P0.7270.66
70_Q152_A0.7200.66
91_L134_L0.7120.65
115_G121_A0.7100.64
85_I149_E0.7100.64
118_D122_S0.7090.64
18_A31_T0.6910.62
49_L59_W0.6760.61
125_R130_L0.6730.60
91_L140_E0.6700.60
59_W65_L0.6690.60
17_V22_Y0.6660.60
134_L137_Y0.6650.59
52_Y67_L0.6550.58
38_F156_T0.6540.58
14_L19_A0.6520.58
42_G154_S0.6510.58
48_D81_H0.6510.58
50_D81_H0.6510.58
4_R74_R0.6490.58
90_R152_A0.6470.57
121_A125_R0.6460.57
16_P19_A0.6440.57
17_V34_F0.6440.57
110_A115_G0.6420.57
162_G167_R0.6420.57
132_L135_Q0.6410.57
7_D46_S0.6410.57
34_F150_L0.6370.56
125_R128_G0.6320.56
8_R30_L0.6320.56
104_L123_L0.6240.55
62_L142_L0.6230.54
102_L107_Y0.6210.54
70_Q83_P0.6170.54
59_W68_P0.6160.54
109_L114_G0.6120.53
3_G148_L0.6050.52
83_P156_T0.6030.52
133_L146_L0.6020.52
29_A33_I0.6010.52
71_K92_L0.6010.52
19_A22_Y0.5990.52
44_M79_L0.5980.52
84_I93_Y0.5970.51
3_G6_H0.5940.51
87_T94_L0.5940.51
10_T148_L0.5920.51
112_I118_D0.5920.51
116_W122_S0.5910.51
58_R68_P0.5910.51
138_P141_L0.5900.51
133_L141_L0.5890.50
73_F79_L0.5840.50
141_L144_L0.5780.49
49_L68_P0.5750.49
41_G152_A0.5730.49
116_W121_A0.5720.48
1_P167_R0.5710.48
48_D75_H0.5690.48
50_D75_H0.5690.48
75_H81_H0.5690.48
37_G151_G0.5660.48
59_W63_R0.5660.48
1_P162_G0.5600.47
98_L128_G0.5490.46
101_L105_L0.5470.45
118_D124_Q0.5460.45
123_L132_L0.5450.45
116_W124_Q0.5440.45
152_A156_T0.5430.45
125_R131_L0.5430.45
56_Y152_A0.5430.45
65_L93_Y0.5430.45
68_P76_R0.5420.45
7_D43_L0.5410.45
121_A132_L0.5410.45
49_L58_R0.5400.45
107_Y111_A0.5380.44
87_T145_L0.5370.44
32_L105_L0.5340.44
98_L114_G0.5320.44
42_G77_S0.5310.44
88_L92_L0.5310.44
119_L123_L0.5270.43
8_R71_K0.5260.43
29_A141_L0.5250.43
14_L38_F0.5240.43
112_I141_L0.5230.43
52_Y57_R0.5220.43
149_E154_S0.5200.42
7_D152_A0.5180.42
127_L133_L0.5170.42
81_H155_H0.5170.42
48_D155_H0.5170.42
50_D155_H0.5170.42
61_P97_L0.5160.42
37_G61_P0.5160.42
22_Y105_L0.5130.41
122_S125_R0.5110.41
116_W120_L0.5080.41
35_A97_L0.5050.41
7_D55_Q0.5020.40
35_A130_L0.5010.40
44_M82_G0.5000.40
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3klyA 3 0.3254 3.9 0.952 Contact Map
2o1qA 4 0.2899 1.8 0.96 Contact Map
4lukA 2 0.3491 1.6 0.961 Contact Map
4fc4A 3 0.3254 1.6 0.961 Contact Map
2b8mA 2 0.1834 1.4 0.963 Contact Map
3tdsE 3 0.3905 1.3 0.963 Contact Map
2ajjA 1 0.142 1.3 0.963 Contact Map
3mp7B 1 0.2189 1.1 0.965 Contact Map
3kcuA 3 0.3314 1 0.966 Contact Map
4ui9G 1 0 1 0.966 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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