GREMLIN Database
DUF2214 - Predicted membrane protein (DUF2214)
PFAM: PF09980 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 146 (146)
Sequences: 4337 (2918)
Seq/√Len: 241.5
META: 0.91

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
58_L62_F3.0931.00
77_W81_A2.8681.00
67_A71_L2.7281.00
6_Y136_L2.7141.00
23_F125_H2.5021.00
43_A47_I2.0701.00
2_A140_L1.9761.00
4_L63_F1.9481.00
140_L145_I1.9211.00
31_D34_A1.8981.00
34_A37_R1.8941.00
74_P78_A1.8921.00
57_L61_F1.8621.00
26_L124_L1.8611.00
86_V134_I1.8431.00
57_L80_M1.7701.00
5_A9_H1.7401.00
35_A39_A1.7401.00
12_I52_V1.7391.00
96_T116_E1.7341.00
50_G54_V1.6491.00
125_H129_A1.5971.00
78_A137_L1.5751.00
51_L55_T1.5371.00
89_L123_I1.5371.00
53_L83_F1.5321.00
107_G110_P1.5271.00
54_V58_L1.5201.00
96_T120_L1.5121.00
133_L137_L1.5101.00
90_S94_T1.5001.00
113_D116_E1.4731.00
16_V131_L1.4441.00
82_L133_L1.4281.00
77_W84_V1.4171.00
46_G49_A1.3991.00
13_F16_V1.3891.00
75_L140_L1.3841.00
60_V67_A1.3751.00
92_Y119_R1.3691.00
92_Y96_T1.3651.00
61_F67_A1.3571.00
11_A51_L1.3411.00
14_L48_A1.3311.00
19_L45_Y1.3191.00
5_A59_R1.3171.00
20_A24_V1.3131.00
10_L132_V1.3041.00
7_L55_T1.2821.00
88_L91_I1.2791.00
45_Y87_G1.2761.00
75_L137_L1.2731.00
39_A101_R1.2721.00
2_A6_Y1.2591.00
112_P117_V1.2431.00
21_A41_A1.2251.00
122_R126_I1.2171.00
17_G21_A1.2081.00
94_T98_I1.2021.00
40_R43_A1.2011.00
38_L97_F1.1991.00
96_T99_R1.1951.00
121_R125_H1.1871.00
17_G20_A1.1711.00
103_A108_A1.1651.00
77_W80_M1.1641.00
98_I101_R1.1511.00
9_H55_T1.1501.00
92_Y123_I1.1461.00
36_R105_R1.1351.00
118_R122_R1.1321.00
82_L130_L1.1261.00
18_A44_V1.1231.00
26_L120_L1.1131.00
18_A48_A1.1081.00
3_L7_L1.1081.00
119_R122_R1.1081.00
11_A52_V1.0831.00
60_V71_L1.0821.00
57_L71_L1.0641.00
71_L80_M1.0451.00
95_V99_R1.0421.00
69_Y74_P1.0401.00
76_F80_M1.0351.00
82_L137_L1.0121.00
114_A118_R1.0121.00
102_K106_A0.9911.00
136_L140_L0.9861.00
9_H13_F0.9761.00
83_F134_I0.9651.00
11_A55_T0.9511.00
9_H12_I0.9491.00
60_V66_G0.9401.00
92_Y120_L0.9391.00
9_H139_A0.9261.00
37_R40_R0.9181.00
81_A85_L0.9091.00
116_E119_R0.9071.00
92_Y95_V0.9031.00
46_G50_G0.9021.00
13_F17_G0.8961.00
47_I50_G0.8911.00
21_A24_V0.8820.99
87_G90_S0.8800.99
88_L92_Y0.8710.99
53_L87_G0.8700.99
74_P145_I0.8660.99
82_L134_I0.8560.99
119_R123_I0.8560.99
75_L78_A0.8440.99
73_N145_I0.8440.99
91_I95_V0.8440.99
103_A107_G0.8370.99
30_L38_L0.8320.99
21_A25_L0.8300.99
86_V130_L0.8280.99
42_D46_G0.8230.99
36_R40_R0.8180.99
63_F66_G0.8180.99
28_P33_A0.8180.99
68_A72_H0.8120.99
114_A117_V0.7980.99
101_R105_R0.7940.99
32_A36_R0.7870.99
58_L63_F0.7870.99
122_R129_A0.7860.99
122_R125_H0.7860.99
45_Y127_E0.7860.99
30_L34_A0.7850.99
43_A46_G0.7820.99
45_Y90_S0.7780.99
80_M84_V0.7780.99
39_A104_L0.7730.99
53_L57_L0.7690.99
22_E45_Y0.7600.98
38_L41_A0.7580.98
68_A71_L0.7540.98
2_A145_I0.7460.98
128_L132_V0.7420.98
78_A82_L0.7400.98
29_E114_A0.7380.98
131_L135_P0.7300.98
39_A98_I0.7260.98
15_L52_V0.7240.98
48_A52_V0.7140.98
12_I15_L0.7080.98
71_L77_W0.7070.98
6_Y9_H0.7060.98
118_R125_H0.6940.97
23_F124_L0.6940.97
125_H128_L0.6930.97
115_A119_R0.6910.97
33_A37_R0.6870.97
34_A38_L0.6850.97
52_V134_I0.6820.97
99_R102_K0.6790.97
1_D4_L0.6760.97
6_Y10_L0.6730.97
139_A143_R0.6680.97
130_L135_P0.6610.96
131_L134_I0.6530.96
132_V135_P0.6480.96
27_R34_A0.6310.95
4_L58_L0.6300.95
70_Y142_A0.6300.95
22_E97_F0.6250.95
2_A5_A0.6220.95
74_P77_W0.6180.95
44_V47_I0.6170.95
14_L44_V0.6140.95
16_V24_V0.6130.94
52_V131_L0.6100.94
6_Y135_P0.6090.94
12_I16_V0.6080.94
18_A41_A0.6070.94
1_D63_F0.5970.94
115_A118_R0.5920.93
40_R44_V0.5910.93
117_V124_L0.5870.93
11_A14_L0.5830.93
83_F87_G0.5830.93
76_F141_M0.5800.93
42_D59_R0.5720.92
28_P31_D0.5680.92
61_F71_L0.5670.92
16_V20_A0.5670.92
19_L22_E0.5670.92
54_V62_F0.5650.92
129_A133_L0.5590.91
84_V87_G0.5590.91
65_K69_Y0.5580.91
54_V57_L0.5580.91
23_F128_L0.5570.91
35_A97_F0.5560.91
127_E132_V0.5550.91
75_L145_I0.5540.91
110_P113_D0.5520.91
138_A143_R0.5490.91
95_V102_K0.5480.90
86_V93_P0.5460.90
15_L48_A0.5450.90
60_V80_M0.5440.90
90_S93_P0.5430.90
1_D64_E0.5350.89
23_F129_A0.5340.89
36_R104_L0.5300.89
120_L123_I0.5290.89
84_V88_L0.5270.89
95_V98_I0.5250.89
59_R100_W0.5220.88
89_L93_P0.5220.88
138_A141_M0.5210.88
73_N146_G0.5190.88
42_D49_A0.5170.88
57_L60_V0.5130.87
3_L136_L0.5100.87
133_L136_L0.5070.87
103_A109_A0.5030.86
43_A98_I0.5000.86
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1fftB 1 0.5479 9.4 0.916 Contact Map
4dx5A 3 0.8699 4.5 0.928 Contact Map
3ne5A 3 0.8767 3.6 0.931 Contact Map
4k0jA 3 0.8288 3.5 0.932 Contact Map
4mt1A 3 0.9041 3 0.934 Contact Map
3w9iA 3 0.8699 2.8 0.935 Contact Map
1q16C 2 0.774 2.7 0.935 Contact Map
4humA 1 0.7945 2.3 0.938 Contact Map
4k1cA 4 0.8699 2.1 0.939 Contact Map
4lz6A 1 0.8082 1.9 0.94 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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