GREMLIN Database
DUF2213 - Uncharacterized protein conserved in bacteria (DUF2213)
PFAM: PF09979 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 167 (162)
Sequences: 3389 (2794)
Seq/√Len: 219.5
META: 0.881

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
125_D144_N3.6761.00
3_R112_E3.2341.00
58_F147_G3.1491.00
23_E41_R2.7301.00
105_A109_D2.5731.00
16_I98_A2.5031.00
127_D143_R2.4471.00
65_D110_A2.3331.00
158_A162_V2.3261.00
55_L96_L2.2771.00
23_E39_I2.2001.00
85_V150_V2.0711.00
78_K104_D2.0551.00
45_P141_I2.0521.00
75_D105_A1.9631.00
82_V107_A1.9111.00
43_Y133_P1.9091.00
109_D113_S1.8801.00
77_W81_A1.8211.00
102_I111_I1.8151.00
15_P97_R1.7821.00
48_E51_K1.7631.00
17_A22_Q1.7471.00
117_E156_G1.6461.00
120_C147_G1.6301.00
128_E140_A1.5971.00
42_V140_A1.5871.00
69_P80_L1.5741.00
132_T136_E1.5701.00
26_G40_V1.5561.00
52_P56_A1.5481.00
15_P95_Y1.5361.00
62_P103_T1.5321.00
41_R139_D1.5271.00
45_P48_E1.5251.00
56_A59_E1.4881.00
95_Y161_D1.4761.00
21_V41_R1.4721.00
65_D107_A1.4631.00
65_D115_K1.4541.00
6_E61_K1.4501.00
120_C158_A1.4331.00
35_D38_K1.4241.00
84_T103_T1.4141.00
81_A103_T1.4001.00
85_V98_A1.3991.00
78_K82_V1.3881.00
156_G162_V1.3761.00
48_E143_R1.3741.00
66_D115_K1.3651.00
26_G30_G1.3481.00
92_D97_R1.3041.00
72_V77_W1.3001.00
22_Q49_V1.2991.00
66_D154_D1.2801.00
4_T8_Y1.2751.00
41_R136_E1.2501.00
10_V101_V1.2461.00
44_R49_V1.2371.00
127_D141_I1.2251.00
50_F91_R1.2151.00
110_A115_K1.1971.00
86_G89_V1.1961.00
63_V102_I1.1881.00
107_A152_L1.1871.00
64_T68_P1.1861.00
58_F120_C1.1861.00
54_S57_S1.1761.00
51_K144_N1.1671.00
82_V104_D1.1371.00
120_C150_V1.1201.00
117_E160_P1.1051.00
88_D99_D1.1031.00
115_K154_D1.0901.00
84_T101_V1.0871.00
130_P139_D1.0651.00
61_K85_V1.0601.00
63_V100_L1.0561.00
132_T135_G1.0521.00
57_S147_G1.0401.00
51_K146_R1.0381.00
127_D130_P1.0301.00
129_T141_I1.0241.00
46_P91_R1.0211.00
72_V80_L1.0191.00
60_G87_E1.0021.00
59_E89_V1.0001.00
54_S145_I0.9991.00
54_S147_G0.9961.00
48_E141_I0.9911.00
131_G138_Y0.9871.00
44_R145_I0.9871.00
61_K147_G0.9801.00
132_T137_P0.9801.00
12_K99_D0.9791.00
116_R163_R0.9741.00
63_V152_L0.9711.00
118_L150_V0.9311.00
111_I152_L0.9240.99
90_R97_R0.9230.99
76_N80_L0.9220.99
133_P136_E0.9100.99
66_D153_V0.9090.99
60_G84_T0.9060.99
87_E99_D0.9000.99
100_L150_V0.9000.99
89_V98_A0.8900.99
126_L140_A0.8880.99
107_A110_A0.8830.99
109_D112_E0.8720.99
62_P81_A0.8640.99
70_E76_N0.8630.99
95_Y162_V0.8590.99
71_L123_T0.8570.99
13_A118_L0.8540.99
40_V140_A0.8450.99
73_T76_N0.8430.99
26_G36_P0.8340.99
64_T72_V0.8230.99
25_L39_I0.8180.99
41_R137_P0.8160.99
74_A108_I0.8150.99
8_Y61_K0.8140.99
53_D146_R0.8120.99
49_V145_I0.8100.99
94_D134_D0.8020.99
5_P146_R0.7940.98
117_E163_R0.7920.98
44_R122_Y0.7910.98
15_P162_V0.7740.98
48_E145_I0.7740.98
42_V126_L0.7690.98
16_I120_C0.7650.98
60_G86_G0.7610.98
106_D110_A0.7590.98
59_E88_D0.7570.98
51_K54_S0.7570.98
8_Y77_W0.7500.98
22_Q44_R0.7500.98
16_I147_G0.7470.98
18_R161_D0.7400.98
59_E98_A0.7380.97
104_D107_A0.7350.97
19_T46_P0.7330.97
70_E80_L0.7290.97
77_W103_T0.7210.97
54_S58_F0.7180.97
100_L118_L0.7180.97
8_Y103_T0.7160.97
43_Y132_T0.7150.97
29_I40_V0.7080.97
27_S37_D0.7070.97
13_A97_R0.7010.97
4_T10_V0.6960.96
58_F96_L0.6940.96
124_C142_Q0.6910.96
26_G34_L0.6900.96
48_E144_N0.6890.96
68_P80_L0.6880.96
34_L38_K0.6850.96
10_V60_G0.6850.96
50_F55_L0.6790.96
49_V54_S0.6740.96
86_G99_D0.6730.96
138_Y141_I0.6710.96
17_A49_V0.6700.96
44_R142_Q0.6690.95
65_D82_V0.6690.95
48_E142_Q0.6690.95
26_G29_I0.6670.95
125_D143_R0.6580.95
70_E73_T0.6540.95
13_A16_I0.6520.95
42_V124_C0.6520.95
29_I42_V0.6510.95
91_R94_D0.6500.95
25_L29_I0.6470.95
21_V43_Y0.6450.95
117_E155_R0.6450.95
159_G162_V0.6360.94
60_G101_V0.6350.94
72_V76_N0.6350.94
34_L37_D0.6290.94
46_P50_F0.6280.94
33_G36_P0.6120.93
69_P79_E0.6120.93
70_E79_E0.6100.93
16_I150_V0.6070.93
9_L102_I0.6000.92
25_L28_E0.5950.92
17_A120_C0.5850.91
81_A84_T0.5850.91
74_A77_W0.5850.91
153_V156_G0.5830.91
82_V152_L0.5820.91
121_G149_H0.5800.91
63_V118_L0.5790.91
68_P121_G0.5780.91
92_D95_Y0.5740.90
44_R166_D0.5720.90
47_E50_F0.5720.90
86_G90_R0.5720.90
85_V118_L0.5710.90
17_A44_R0.5710.90
21_V139_D0.5690.90
8_Y62_P0.5690.90
74_A105_A0.5680.90
129_T139_D0.5680.90
90_R93_G0.5660.90
68_P72_V0.5650.90
10_V61_K0.5630.90
102_I152_L0.5610.89
22_Q120_C0.5590.89
111_I164_I0.5560.89
19_T45_P0.5540.89
3_R9_L0.5500.88
59_E87_E0.5460.88
16_I85_V0.5460.88
130_P137_P0.5380.87
26_G35_D0.5370.87
110_A113_S0.5350.87
17_A96_L0.5330.87
18_R117_E0.5240.86
87_E91_R0.5210.86
22_Q158_A0.5200.86
19_T43_Y0.5200.86
11_V100_L0.5200.86
63_V150_V0.5160.85
106_D109_D0.5160.85
96_L147_G0.5150.85
156_G163_R0.5140.85
30_G40_V0.5130.85
43_Y141_I0.5100.84
108_I112_E0.5100.84
45_P143_R0.5080.84
129_T143_R0.5080.84
105_A108_I0.5070.84
96_L120_C0.5060.84
77_W104_D0.5020.83
19_T49_V0.5010.83
32_L35_D0.5010.83
6_E10_V0.5010.83
63_V82_V0.5000.83
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4v08A 2 0.7186 41.8 0.923 Contact Map
2pbkA 2 0.7246 21.2 0.933 Contact Map
4p3hA 4 0.7186 20.6 0.934 Contact Map
1at3A 3 0.7365 19.7 0.935 Contact Map
1vzvA 2 0.7066 19.2 0.935 Contact Map
1iedA 2 0.7126 16.3 0.937 Contact Map
1o6eA 4 0.7665 15.5 0.937 Contact Map
4wsbC 1 0.7246 12 0.941 Contact Map
4ct3A 4 0.6467 4.6 0.951 Contact Map
1tnsA 1 0.4311 2.9 0.956 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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