GREMLIN Database
DUF2199 - Uncharacterized protein conserved in bacteria (DUF2199)
PFAM: PF09965 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 146 (146)
Sequences: 2906 (2424)
Seq/√Len: 200.6
META: 0.897

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
104_R110_D3.3121.00
34_R45_D2.7901.00
4_C9_H2.6941.00
76_E80_E2.6251.00
53_G129_I2.4211.00
22_Y102_S2.3711.00
121_R128_L2.2901.00
116_T129_I2.1311.00
82_Y86_W2.1161.00
14_D67_G2.0971.00
108_Y141_E2.0751.00
85_L94_E2.0571.00
23_Y35_A2.0351.00
38_T41_L1.9311.00
56_E65_P1.8911.00
81_R94_E1.8661.00
119_H130_E1.8441.00
48_H73_S1.7671.00
17_F103_N1.7621.00
56_E67_G1.7601.00
140_V144_N1.7401.00
52_R69_G1.7131.00
81_R85_L1.7061.00
41_L83_L1.7051.00
72_V99_G1.6661.00
1_K8_E1.6611.00
72_V129_I1.6421.00
111_T114_L1.6091.00
54_V69_G1.5711.00
117_R130_E1.5141.00
54_V67_G1.5121.00
26_L35_A1.5121.00
1_K6_G1.4961.00
75_S78_N1.4881.00
101_L116_T1.4711.00
56_E121_R1.4271.00
99_G118_V1.4191.00
92_E122_P1.4121.00
117_R132_E1.3881.00
55_L70_V1.3741.00
80_E84_E1.3711.00
115_K132_E1.3691.00
134_T139_A1.3521.00
102_S113_N1.3521.00
72_V116_T1.3501.00
21_D103_N1.3491.00
97_Y120_L1.3161.00
30_E34_R1.3051.00
132_E139_A1.2941.00
19_A23_Y1.2901.00
51_V82_Y1.2891.00
40_D126_R1.2701.00
19_A37_L1.2611.00
114_L136_H1.2461.00
81_R95_P1.2081.00
100_W115_K1.1641.00
85_L88_D1.1611.00
78_N98_F1.1471.00
41_L82_Y1.1381.00
26_L31_R1.1361.00
139_A143_R1.1331.00
72_V118_V1.1231.00
81_R84_E1.1221.00
99_G116_T1.1121.00
17_F105_L1.1111.00
23_Y26_L1.0941.00
26_L34_R1.0931.00
70_V129_I1.0921.00
56_E128_L1.0671.00
62_S65_P1.0441.00
137_P140_V1.0371.00
57_I138_L1.0301.00
59_V65_P1.0201.00
43_V79_F1.0141.00
55_L101_L1.0141.00
69_G126_R1.0061.00
70_V101_L1.0041.00
135_D140_V1.0001.00
21_D25_A0.9941.00
22_Y48_H0.9881.00
22_Y112_L0.9881.00
78_N100_W0.9811.00
2_C5_C0.9730.99
74_L118_V0.9670.99
109_P137_P0.9620.99
51_V74_L0.9560.99
73_S100_W0.9460.99
21_D112_L0.9350.99
21_D24_L0.9340.99
14_D69_G0.9130.99
98_F117_R0.9060.99
131_L142_Q0.9050.99
73_S78_N0.9010.99
73_S98_F0.8950.99
90_G122_P0.8920.99
94_E97_Y0.8890.99
8_E11_G0.8820.99
57_I68_W0.8820.99
133_P139_A0.8720.99
37_L42_C0.8630.99
68_W141_E0.8600.99
47_E76_E0.8600.99
96_P117_R0.8550.99
44_I48_H0.8540.99
54_V126_R0.8530.99
36_E43_V0.8400.99
105_L111_T0.8380.98
43_V49_F0.8360.98
27_P30_E0.8270.98
137_P141_E0.8190.98
74_L120_L0.8180.98
41_L86_W0.8160.98
74_L97_Y0.8160.98
121_R125_Q0.8150.98
15_F68_W0.8140.98
19_A24_L0.8130.98
140_V143_R0.8070.98
98_F116_T0.8070.98
49_F76_E0.8040.98
45_D49_F0.8020.98
78_N97_Y0.7800.98
22_Y113_N0.7730.97
91_R123_V0.7730.97
86_W123_V0.7730.97
17_F69_G0.7670.97
100_W113_N0.7660.97
35_A44_I0.7610.97
23_Y37_L0.7610.97
49_F79_F0.7590.97
9_H12_L0.7580.97
89_P123_V0.7470.97
16_G52_R0.7440.97
98_F115_K0.7430.97
120_L123_V0.7430.97
18_D24_L0.7410.97
114_L131_L0.7390.97
90_G93_H0.7380.97
1_K4_C0.7310.96
78_N118_V0.7300.96
88_D92_E0.7300.96
70_V105_L0.7300.96
122_P125_Q0.7190.96
82_Y120_L0.7160.96
80_E83_L0.7160.96
134_T140_V0.7120.96
23_Y44_I0.7090.96
7_E11_G0.7070.96
29_E32_E0.7040.96
141_E146_I0.7010.96
68_W108_Y0.6980.95
28_E31_R0.6970.95
11_G14_D0.6900.95
50_F103_N0.6870.95
41_L79_F0.6840.95
91_R122_P0.6730.94
50_F102_S0.6690.94
51_V118_V0.6690.94
48_H102_S0.6620.94
95_P120_L0.6600.94
26_L44_I0.6600.94
61_G65_P0.6530.93
91_R124_G0.6500.93
138_L142_Q0.6470.93
24_L27_P0.6380.93
68_W107_G0.6370.93
50_F73_S0.6290.92
96_P130_E0.6250.92
38_T83_L0.6180.91
31_R35_A0.6160.91
75_S100_W0.6090.91
70_V103_N0.6080.91
16_G69_G0.6050.91
84_E87_D0.5990.90
53_G72_V0.5970.90
118_V129_I0.5940.90
111_T136_H0.5940.90
14_D54_V0.5900.90
86_W89_P0.5900.90
60_I65_P0.5900.90
92_E123_V0.5890.89
22_Y50_F0.5890.89
96_P119_H0.5890.89
39_S86_W0.5880.89
77_E80_E0.5880.89
49_F80_E0.5880.89
24_L31_R0.5850.89
35_A45_D0.5850.89
3_S8_E0.5840.89
53_G67_G0.5830.89
85_L95_P0.5750.88
131_L138_L0.5730.88
131_L139_A0.5660.88
34_R44_I0.5650.87
34_R46_G0.5650.87
23_Y31_R0.5640.87
21_D104_R0.5610.87
24_L34_R0.5580.87
132_E140_V0.5550.87
39_S126_R0.5550.87
125_Q128_L0.5530.86
64_E67_G0.5520.86
133_P143_R0.5510.86
36_E83_L0.5490.86
115_K139_A0.5460.86
27_P31_R0.5440.85
28_E32_E0.5390.85
115_K134_T0.5380.85
26_L45_D0.5360.85
54_V121_R0.5320.84
66_F142_Q0.5250.83
90_G123_V0.5250.83
26_L29_E0.5200.83
46_G76_E0.5150.82
14_D65_P0.5150.82
3_S7_E0.5080.81
44_I73_S0.5060.81
73_S116_T0.5050.81
132_E143_R0.5010.80
91_R120_L0.5000.80
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1gh9A 1 0.2945 11.2 0.934 Contact Map
2k5cA 1 0.2877 10 0.935 Contact Map
2f9yB 1 0.8151 9.9 0.935 Contact Map
1twfL 1 0.1644 9.1 0.936 Contact Map
2ej4A 1 0.2192 8 0.938 Contact Map
3py7A 1 0.2877 7.6 0.938 Contact Map
2pk7A 2 0.2671 7.5 0.939 Contact Map
2lvhA 1 0.0822 6.7 0.94 Contact Map
2m4yA 1 0.1301 6.5 0.94 Contact Map
2jnyA 1 0.2192 6.3 0.941 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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