GREMLIN Database
DUF2169 - Uncharacterized protein conserved in bacteria (DUF2169)
PFAM: PF09937 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 294 (290)
Sequences: 3968 (3512)
Seq/√Len: 206.2
META: 0.782

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
96_D160_N3.5011.00
89_K224_D3.2251.00
66_H285_D2.9591.00
79_D92_R2.9241.00
239_L242_L2.8701.00
234_G255_G2.8091.00
75_V78_L2.6881.00
91_L161_I2.6501.00
199_E203_K2.6201.00
78_L241_N2.6171.00
200_A204_E2.5361.00
237_V251_F2.5361.00
66_H116_R2.5221.00
81_G90_T2.4901.00
114_F117_M2.3851.00
96_D112_E2.2991.00
83_R88_D2.2781.00
205_R211_E2.2751.00
81_G88_D2.2631.00
63_L82_L2.2611.00
69_A75_V2.2611.00
117_M160_N2.2371.00
83_R238_E2.2331.00
45_S122_E2.2221.00
238_E250_R2.1861.00
188_P192_R2.1501.00
123_R144_R2.1081.00
78_L114_F2.0851.00
68_Y116_R2.0501.00
193_L216_R2.0191.00
79_D90_T1.9751.00
277_L291_L1.9671.00
81_G240_E1.9521.00
161_I179_G1.9231.00
77_A92_R1.9221.00
278_D294_R1.9141.00
74_P115_T1.9101.00
96_D123_R1.8601.00
123_R160_N1.8551.00
222_P225_Q1.8241.00
260_V275_L1.7931.00
59_T231_L1.7881.00
38_L47_R1.7801.00
108_P158_L1.7751.00
144_R157_P1.7511.00
56_K279_T1.7451.00
15_D25_A1.7401.00
58_R226_Q1.7391.00
62_L182_P1.7151.00
236_P250_R1.7041.00
238_E248_R1.6721.00
283_D286_A1.6471.00
236_P252_R1.6371.00
80_V91_L1.6351.00
91_L178_A1.6321.00
120_R123_R1.6261.00
63_L242_L1.6221.00
58_R216_R1.6201.00
163_D178_A1.6131.00
264_R271_E1.6101.00
49_E185_R1.6101.00
237_V282_I1.6021.00
61_V82_L1.5891.00
117_M123_R1.5231.00
240_E248_R1.5161.00
76_T110_E1.5061.00
196_T212_D1.4981.00
89_K225_Q1.4971.00
94_F167_P1.4821.00
166_A176_R1.4641.00
188_P191_A1.4581.00
139_R176_R1.4301.00
283_D288_R1.3991.00
230_Y276_R1.3761.00
38_L120_R1.3621.00
102_G105_G1.3561.00
80_V180_F1.3551.00
58_R228_P1.3421.00
259_R272_E1.3321.00
87_L227_L1.3271.00
62_L279_T1.3261.00
58_R229_G1.3181.00
91_L180_F1.3051.00
15_D288_R1.2891.00
84_V237_V1.2831.00
213_F218_F1.2691.00
118_P123_R1.2611.00
16_L249_L1.2591.00
261_F270_F1.2591.00
25_A28_Q1.2561.00
36_E47_R1.2521.00
67_A78_L1.2501.00
41_P44_S1.2411.00
92_R167_P1.2281.00
57_P219_N1.2201.00
94_F111_P1.2181.00
64_V281_W1.2011.00
138_P191_A1.1981.00
261_F272_E1.1971.00
68_Y244_P1.1931.00
66_H118_P1.1911.00
58_R219_N1.1901.00
199_E202_L1.1871.00
78_L117_M1.1831.00
121_Y187_W1.1801.00
253_L280_V1.1611.00
68_Y285_D1.1581.00
35_D49_E1.1541.00
245_E287_E1.1531.00
46_L49_E1.1521.00
62_L220_A1.1481.00
77_A94_F1.1241.00
251_F282_I1.1181.00
142_V187_W1.1171.00
67_A80_V1.1161.00
54_P218_F1.1151.00
96_D117_M1.0991.00
193_L223_P1.0991.00
227_L231_L1.0981.00
185_R217_F1.0981.00
163_D166_A1.0971.00
213_F217_F1.0941.00
98_V109_S1.0841.00
121_Y222_P1.0791.00
239_L282_I1.0681.00
243_H247_G1.0681.00
13_T28_Q1.0631.00
15_D26_E1.0611.00
97_R162_E1.0531.00
231_L280_V1.0501.00
59_T230_Y1.0491.00
64_V182_P1.0451.00
281_W292_V1.0451.00
60_D226_Q1.0451.00
194_A217_F1.0421.00
232_R235_E1.0391.00
182_P220_A1.0321.00
63_L180_F1.0301.00
99_W168_I1.0251.00
277_L293_W1.0251.00
262_V273_V1.0221.00
69_A115_T1.0201.00
121_Y179_G1.0201.00
12_A293_W1.0151.00
169_T173_D1.0151.00
95_G111_P1.0061.00
11_K51_D1.0041.00
162_E168_I1.0001.00
241_N247_G1.0001.00
62_L221_A0.9991.00
234_G253_L0.9971.00
138_P188_P0.9931.00
197_Y201_W0.9911.00
216_R226_Q0.9891.00
40_E44_S0.9881.00
45_S120_R0.9811.00
193_L226_Q0.9761.00
60_D231_L0.9721.00
197_Y212_D0.9721.00
67_A93_V0.9721.00
51_D292_V0.9691.00
166_A173_D0.9681.00
13_T25_A0.9661.00
39_G44_S0.9641.00
249_L287_E0.9621.00
137_Y146_F0.9540.99
126_G145_G0.9520.99
40_E43_L0.9500.99
15_D23_R0.9480.99
163_D176_R0.9420.99
36_E48_Y0.9310.99
98_V112_E0.9300.99
119_L161_I0.9250.99
71_G74_P0.9220.99
46_L184_G0.9220.99
193_L214_D0.9210.99
55_F230_Y0.9210.99
60_D219_N0.9180.99
243_H249_L0.9180.99
189_P192_R0.9170.99
219_N226_Q0.9150.99
243_H287_E0.9150.99
61_V87_L0.9130.99
109_S158_L0.9100.99
282_I289_V0.9100.99
139_R189_P0.9090.99
83_R236_P0.9090.99
280_V289_V0.9070.99
198_D201_W0.9060.99
131_P134_H0.9010.99
14_F256_L0.9000.99
48_Y283_D0.8960.99
125_F145_G0.8890.99
202_L206_F0.8870.99
129_P134_H0.8860.99
82_L225_Q0.8860.99
119_L242_L0.8850.99
13_T30_P0.8830.99
8_V260_V0.8830.99
92_R162_E0.8770.99
128_D134_H0.8750.99
93_V161_I0.8740.99
38_L45_S0.8710.99
195_G212_D0.8620.99
82_L180_F0.8610.99
55_F215_P0.8590.99
183_I187_W0.8580.99
61_V227_L0.8480.99
16_L251_F0.8440.99
119_L180_F0.8440.99
65_G285_D0.8430.99
201_W207_P0.8420.99
201_W212_D0.8360.99
62_L281_W0.8350.99
114_F160_N0.8270.99
277_L280_V0.8270.99
96_D157_P0.8240.98
230_Y235_E0.8230.98
230_Y233_G0.8160.98
95_G109_S0.8160.98
222_P226_Q0.8150.98
29_E288_R0.8090.98
184_G187_W0.8080.98
98_V157_P0.8080.98
46_L182_P0.7970.98
144_R160_N0.7960.98
263_R270_F0.7950.98
99_W108_P0.7930.98
17_P23_R0.7910.98
49_E220_A0.7900.98
168_I175_P0.7880.98
14_F289_V0.7870.98
59_T280_V0.7870.98
183_I189_P0.7850.98
96_D114_F0.7830.98
251_F289_V0.7780.98
200_A211_E0.7770.98
158_L167_P0.7770.98
87_L225_Q0.7770.98
84_V280_V0.7760.98
125_F146_F0.7740.98
38_L122_E0.7730.98
283_D290_S0.7720.98
10_V258_P0.7720.98
35_D50_S0.7720.98
128_D133_K0.7710.98
33_L36_E0.7700.98
281_W290_S0.7660.98
162_E167_P0.7650.98
94_F158_L0.7600.97
265_R268_G0.7580.97
17_P20_G0.7570.97
7_V278_D0.7570.97
239_L280_V0.7570.97
167_P175_P0.7550.97
168_I173_D0.7490.97
69_A78_L0.7440.97
209_L218_F0.7440.97
235_E253_L0.7360.97
33_L52_L0.7360.97
32_V36_E0.7350.97
125_F137_Y0.7330.97
116_R285_D0.7310.97
196_T201_W0.7300.97
81_G238_E0.7280.97
17_P21_E0.7230.97
141_P187_W0.7220.96
49_E182_P0.7190.96
233_G255_G0.7180.96
121_Y181_G0.7150.96
221_A226_Q0.7150.96
147_A156_L0.7150.96
101_R106_L0.7140.96
201_W205_R0.7090.96
75_V114_F0.7060.96
130_D134_H0.7060.96
31_L52_L0.7060.96
68_Y242_L0.7030.96
82_L282_I0.6960.96
200_A205_R0.6960.96
65_G82_L0.6930.96
69_A74_P0.6930.96
89_K222_P0.6900.95
64_V283_D0.6860.95
59_T227_L0.6860.95
205_R208_L0.6860.95
280_V291_L0.6840.95
94_F97_R0.6830.95
59_T277_L0.6820.95
257_R274_P0.6820.95
100_R103_G0.6820.95
170_S173_D0.6810.95
61_V282_I0.6800.95
89_K178_A0.6800.95
67_A114_F0.6790.95
63_L284_P0.6780.95
256_L293_W0.6740.95
201_W210_P0.6730.95
245_E249_L0.6730.95
68_Y78_L0.6720.95
235_E255_G0.6710.95
92_R164_P0.6670.95
210_P213_F0.6650.94
94_F168_I0.6650.94
76_T113_P0.6630.94
216_R223_P0.6630.94
57_P215_P0.6610.94
92_R165_D0.6570.94
64_V181_G0.6540.94
77_A167_P0.6540.94
80_V93_V0.6510.94
65_G239_L0.6490.94
258_P273_V0.6470.94
197_Y210_P0.6460.94
39_G43_L0.6430.93
48_Y64_V0.6420.93
130_D133_K0.6420.93
54_P215_P0.6420.93
139_R188_P0.6420.93
17_P22_L0.6410.93
37_Y46_L0.6410.93
16_L287_E0.6410.93
59_T229_G0.6400.93
258_P262_V0.6370.93
163_D167_P0.6370.93
91_L164_P0.6370.93
136_A146_F0.6360.93
183_I226_Q0.6350.93
266_D269_R0.6350.93
32_V35_D0.6320.93
127_G145_G0.6300.93
58_R230_Y0.6280.93
89_K163_D0.6270.93
37_Y41_P0.6240.92
147_A153_A0.6240.92
194_A197_Y0.6240.92
37_Y285_D0.6210.92
39_G42_G0.6180.92
71_G115_T0.6160.92
151_S154_G0.6120.92
80_V242_L0.6100.92
37_Y44_S0.6100.92
82_L239_L0.6090.91
231_L235_E0.6050.91
89_K180_F0.6040.91
137_Y177_P0.6010.91
122_E142_V0.5990.91
9_V292_V0.5990.91
16_L22_L0.5970.91
62_L222_P0.5910.90
197_Y209_L0.5910.90
32_V50_S0.5900.90
16_L289_V0.5870.90
121_Y177_P0.5870.90
35_D185_R0.5870.90
11_K31_L0.5840.90
56_K60_D0.5780.89
223_P226_Q0.5740.89
10_V275_L0.5720.89
49_E53_A0.5710.89
163_D224_D0.5650.88
140_N143_G0.5640.88
99_W171_P0.5640.88
149_R153_A0.5640.88
22_L289_V0.5630.88
9_V294_R0.5620.88
234_G252_R0.5610.88
197_Y213_F0.5600.88
129_P132_A0.5590.88
81_G248_R0.5570.87
99_W158_L0.5540.87
135_A146_F0.5530.87
78_L93_V0.5510.87
65_G119_L0.5500.87
10_V293_W0.5500.87
215_P219_N0.5490.87
125_F173_D0.5480.87
162_E175_P0.5480.87
149_R154_G0.5460.86
68_Y241_N0.5430.86
90_T165_D0.5420.86
53_A294_R0.5410.86
47_R120_R0.5410.86
216_R219_N0.5410.86
258_P275_L0.5400.86
100_R104_G0.5400.86
117_M161_I0.5390.86
91_L242_L0.5370.85
97_R111_P0.5370.85
186_H191_A0.5370.85
63_L119_L0.5370.85
63_L84_V0.5350.85
24_L28_Q0.5340.85
11_K28_Q0.5330.85
45_S142_V0.5320.85
62_L219_N0.5300.85
48_Y281_W0.5300.85
91_L119_L0.5290.85
187_W190_R0.5290.85
6_L262_V0.5280.85
192_R223_P0.5270.84
51_D56_K0.5260.84
202_L205_R0.5240.84
139_R177_P0.5230.84
242_L248_R0.5220.84
60_D225_Q0.5220.84
191_A194_A0.5210.84
278_D292_V0.5210.84
189_P223_P0.5200.84
142_V184_G0.5190.84
284_P288_R0.5180.83
89_K223_P0.5170.83
128_D135_A0.5170.83
57_P230_Y0.5160.83
96_D144_R0.5160.83
158_L162_E0.5150.83
150_R154_G0.5150.83
60_D227_L0.5140.83
125_F175_P0.5130.83
85_G236_P0.5120.83
90_T240_E0.5100.82
76_T112_E0.5090.82
69_A114_F0.5080.82
197_Y207_P0.5080.82
85_G237_V0.5060.82
67_A241_N0.5050.82
10_V260_V0.5050.82
144_R158_L0.5040.82
196_T199_E0.5030.82
196_T210_P0.5020.81
231_L277_L0.5020.81
63_L239_L0.5020.81
73_R115_T0.5010.81
116_R244_P0.5000.81
90_T164_P0.5000.81
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2blfA 1 0.5 7.8 0.968 Contact Map
1ogpA 4 0.5034 5.9 0.97 Contact Map
2a9dA 2 0.517 3.9 0.972 Contact Map
1soxA 2 0.517 3.6 0.973 Contact Map
4f7uQ 1 0.1939 3.1 0.974 Contact Map
4hhrA 1 0.534 2.9 0.974 Contact Map
4b2hA 5 0.0544 2.7 0.974 Contact Map
2miiA 1 0.3333 2.5 0.975 Contact Map
3vzaE 1 0.1054 2.5 0.975 Contact Map
3lzqA 2 0.4354 2 0.976 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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