GREMLIN Database
DUF2165 - Predicted small integral membrane protein (DUF2165)
PFAM: PF09933 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 157 (155)
Sequences: 2808 (1989)
Seq/√Len: 159.7
META: 0.84

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
17_L79_W4.1201.00
10_A82_A3.0981.00
86_L89_L2.9171.00
78_C106_G2.8201.00
4_A108_T2.6741.00
27_Y61_H2.5741.00
70_W113_L2.5691.00
13_A79_W2.5111.00
20_A72_A2.4361.00
110_G147_I2.2981.00
49_L127_L2.1921.00
43_T47_N2.1791.00
92_P95_A2.1581.00
145_T149_L2.1531.00
31_Y60_H2.1311.00
36_H41_D2.1191.00
32_Q35_R2.0941.00
9_V103_A2.0531.00
25_T51_W2.0291.00
17_L75_A2.0091.00
28_G32_Q1.9721.00
4_A153_F1.9161.00
31_Y61_H1.8721.00
43_T50_M1.8371.00
141_F145_T1.7711.00
80_I102_L1.7101.00
11_A116_V1.7081.00
21_F51_W1.7001.00
22_N127_L1.6461.00
13_A17_L1.5891.00
21_F25_T1.5591.00
138_E142_R1.5481.00
47_N50_M1.5321.00
39_S60_H1.4871.00
139_A142_R1.4771.00
103_A154_V1.4641.00
16_A74_T1.4441.00
84_R87_R1.4081.00
97_N100_K1.4001.00
42_T131_S1.3701.00
15_F19_V1.3621.00
12_V78_C1.3591.00
15_F143_F1.3491.00
74_T109_L1.3331.00
27_Y64_Y1.3321.00
61_H65_A1.3181.00
104_I155_A1.3111.00
39_S55_T1.3071.00
54_I59_L1.2871.00
27_Y68_I1.2601.00
85_L99_A1.2361.00
122_G128_M1.2311.00
77_L105_A1.2181.00
58_A62_A1.2171.00
11_A112_L1.1991.00
96_F100_K1.1961.00
64_Y68_I1.1931.00
74_T113_L1.1651.00
14_L116_V1.1571.00
33_F136_G1.1561.00
149_L152_I1.1481.00
84_R102_L1.1381.00
77_L109_L1.1311.00
139_A143_F1.1311.00
85_L97_N1.1271.00
6_I89_L1.1121.00
20_A75_A1.1111.00
27_Y31_Y1.0890.99
146_V149_L1.0820.99
63_A117_G1.0760.99
46_G50_M1.0640.99
80_I84_R1.0620.99
74_T110_G1.0570.99
34_V67_I1.0550.99
26_D29_S1.0520.99
78_C107_L1.0410.99
36_H42_T1.0380.99
93_A97_N1.0380.99
8_L150_V1.0350.99
75_A79_W1.0270.99
22_N25_T1.0260.99
30_N64_Y1.0240.99
84_R88_A1.0130.99
84_R101_R1.0060.99
38_L63_A0.9730.99
70_W109_L0.9690.99
12_V147_I0.9650.99
104_I151_L0.9610.99
24_I72_A0.9580.99
111_F147_I0.9570.99
127_L130_Q0.9570.99
20_A24_I0.9540.99
115_F120_V0.9520.99
80_I83_V0.9500.99
83_V86_L0.9280.98
44_F130_Q0.9220.98
40_M53_A0.9160.98
91_A95_A0.9040.98
81_G106_G0.9020.98
71_E114_W0.8890.98
35_R39_S0.8830.98
86_L155_A0.8780.98
144_F148_G0.8640.97
9_V154_V0.8630.97
3_L6_I0.8600.97
37_V136_G0.8480.97
70_W117_G0.8450.97
131_S134_W0.8380.97
14_L18_L0.8310.97
116_V120_V0.8260.97
132_K138_E0.8250.97
70_W74_T0.8250.97
7_L112_L0.8240.97
29_S48_A0.8180.96
103_A106_G0.8120.96
137_Q141_F0.7950.96
76_L80_I0.7930.96
110_G113_L0.7900.96
88_A91_A0.7860.95
107_L151_L0.7840.95
38_L122_G0.7800.95
6_I86_L0.7770.95
2_R5_K0.7770.95
16_A120_V0.7750.95
138_E141_F0.7730.95
128_M140_A0.7690.95
82_A86_L0.7630.95
16_A110_G0.7540.94
115_F146_V0.7500.94
81_G103_A0.7390.94
74_T106_G0.7360.94
21_F24_I0.7190.93
78_C81_G0.7180.93
14_L120_V0.7140.93
21_F49_L0.7050.92
51_W125_W0.6960.92
27_Y30_N0.6930.91
4_A104_I0.6890.91
26_D30_N0.6890.91
152_I156_Q0.6880.91
38_L117_G0.6860.91
43_T127_L0.6850.91
85_L96_F0.6810.91
3_L7_L0.6750.90
123_G137_Q0.6750.90
119_M137_Q0.6710.90
8_L111_F0.6680.90
137_Q142_R0.6640.90
128_M135_N0.6580.89
124_E128_M0.6540.89
119_M146_V0.6510.89
33_F134_W0.6470.88
16_A75_A0.6430.88
148_G152_I0.6410.88
141_F146_V0.6310.87
144_F150_V0.6290.87
76_L91_A0.6280.87
98_R101_R0.6270.87
36_H134_W0.6240.86
29_S127_L0.6210.86
88_A92_P0.6200.86
146_V150_V0.6190.86
111_F144_F0.6190.86
94_A125_W0.6160.86
6_I9_V0.6140.86
88_A99_A0.6130.85
40_M125_W0.6130.85
45_P50_M0.6100.85
149_L156_Q0.6100.85
10_A14_L0.6050.85
12_V150_V0.6030.85
15_F120_V0.6020.84
143_F147_I0.5960.84
35_R57_P0.5950.84
29_S49_L0.5940.84
26_D40_M0.5910.83
12_V107_L0.5900.83
8_L148_G0.5880.83
90_R94_A0.5850.83
30_N41_D0.5850.83
104_I108_T0.5820.82
30_N33_F0.5810.82
23_N71_E0.5810.82
6_I85_L0.5800.82
16_A114_W0.5770.82
143_F146_V0.5750.82
22_N137_Q0.5690.81
71_E124_E0.5690.81
112_L116_V0.5670.81
12_V18_L0.5630.80
46_G49_L0.5620.80
16_A115_F0.5610.80
43_T48_A0.5600.80
52_R125_W0.5560.80
79_W82_A0.5550.79
28_G35_R0.5550.79
73_L109_L0.5530.79
50_M53_A0.5520.79
110_G114_W0.5520.79
105_A109_L0.5520.79
49_L52_R0.5520.79
90_R101_R0.5500.79
111_F148_G0.5500.79
71_E113_L0.5500.79
2_R96_F0.5490.79
95_A99_A0.5480.79
146_V154_V0.5480.79
58_A89_L0.5410.78
111_F150_V0.5410.78
6_I82_A0.5390.77
77_L102_L0.5380.77
88_A95_A0.5330.77
133_T136_G0.5310.76
31_Y68_I0.5280.76
148_G153_F0.5280.76
22_N49_L0.5270.76
137_Q143_F0.5230.75
18_L120_V0.5210.75
72_A76_L0.5170.74
7_L80_I0.5150.74
19_V30_N0.5140.74
3_L89_L0.5110.74
124_E131_S0.5100.73
10_A79_W0.5080.73
24_I65_A0.5080.73
117_G120_V0.5070.73
103_A107_L0.5060.73
133_T137_Q0.5050.73
9_V106_G0.5050.73
97_N101_R0.5040.73
11_A14_L0.5030.72
114_W119_M0.5000.72
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4or2A 2 0.8344 6.7 0.944 Contact Map
2h88C 2 0.7898 2.6 0.954 Contact Map
1zoyC 1 0.7771 2.5 0.955 Contact Map
4od4A 1 0.8854 2.4 0.955 Contact Map
4ytpC 1 0.7134 2.3 0.955 Contact Map
1m56D 1 0.1465 1.2 0.962 Contact Map
1qleD 1 0.1465 1.2 0.962 Contact Map
4oo9A 1 0.3949 1.1 0.963 Contact Map
5a63B 1 0.6051 1.1 0.963 Contact Map
2wdqC 1 0.6752 0.9 0.965 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0063 seconds.