GREMLIN Database
DUF2164 - Uncharacterized conserved protein (DUF2164)
PFAM: PF09932 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 74 (73)
Sequences: 5021 (3324)
Seq/√Len: 389.1
META: 0.885

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
40_L47_I2.7451.00
19_F27_I2.5541.00
14_S42_E2.5321.00
48_Y54_D2.2011.00
19_F25_E2.0781.00
8_R33_E1.9181.00
60_Q64_E1.8391.00
17_R21_E1.7711.00
51_A55_A1.7691.00
62_R65_D1.7661.00
4_S7_E1.7001.00
30_L34_L1.6641.00
47_I51_A1.6551.00
16_Q29_D1.6271.00
23_L31_A1.5811.00
15_I36_L1.5201.00
18_Y34_L1.5111.00
20_A26_E1.5001.00
5_K9_A1.4011.00
18_Y35_L1.3911.00
11_L43_I1.3611.00
7_E50_Q1.3451.00
19_F23_L1.3241.00
56_Q60_Q1.3211.00
68_G71_Y1.3201.00
35_L39_F1.3061.00
15_I39_F1.2021.00
52_V70_I1.1851.00
65_D68_G1.1431.00
44_G47_I1.1381.00
10_Q14_S1.1371.00
12_I33_E1.1251.00
3_L11_L1.1061.00
50_Q54_D1.1021.00
6_E9_A1.0971.00
57_A61_E1.0891.00
60_Q67_D1.0711.00
28_G31_A1.0611.00
18_Y38_F1.0551.00
59_L63_V1.0521.00
37_D41_E1.0451.00
13_A16_Q0.9801.00
25_E31_A0.9701.00
19_F35_L0.9691.00
16_Q20_A0.9671.00
61_E65_D0.9671.00
53_A56_Q0.9531.00
31_A34_L0.9281.00
10_Q13_A0.9081.00
7_E46_Y0.9081.00
50_Q53_A0.9011.00
8_R37_D0.8951.00
13_A17_R0.8871.00
56_Q67_D0.8811.00
57_A60_Q0.8781.00
69_E72_E0.8661.00
18_Y22_E0.8521.00
64_E67_D0.8441.00
9_A13_A0.8301.00
58_Y61_E0.8231.00
6_E10_Q0.8201.00
61_E64_E0.8111.00
12_I36_L0.8061.00
18_Y23_L0.8031.00
62_R69_E0.7981.00
14_S17_R0.7871.00
5_K8_R0.7841.00
52_V55_A0.7641.00
58_Y69_E0.7601.00
11_L39_F0.7591.00
58_Y70_I0.6961.00
43_I46_Y0.6830.99
2_E5_K0.6790.99
8_R12_I0.6730.99
51_A70_I0.6670.99
39_F47_I0.6670.99
3_L46_Y0.6630.99
3_L7_E0.6600.99
69_E73_L0.6590.99
63_V66_L0.6580.99
16_Q30_L0.6550.99
17_R20_A0.6460.99
58_Y62_R0.6410.99
15_I27_I0.6190.99
27_I31_A0.6160.99
7_E10_Q0.6130.99
53_A57_A0.6110.99
68_G72_E0.5920.98
14_S38_F0.5850.98
3_L40_L0.5750.98
67_D71_Y0.5750.98
16_Q26_E0.5720.98
65_D69_E0.5720.98
63_V67_D0.5670.98
34_L39_F0.5580.98
68_G73_L0.5540.98
38_F42_E0.5530.98
48_Y51_A0.5420.97
60_Q63_V0.5340.97
12_I32_A0.5340.97
11_L46_Y0.5310.97
7_E11_L0.5270.97
14_S40_L0.5260.97
36_L43_I0.5240.97
3_L44_G0.5200.96
34_L37_D0.5180.96
29_D33_E0.5180.96
30_L37_D0.5080.96
27_I32_A0.5050.96
9_A29_D0.5000.96
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4nguA 1 0.6216 20.1 0.863 Contact Map
2mjgA 2 0.3108 18.3 0.865 Contact Map
3m1gA 2 1 10.4 0.879 Contact Map
4n4uA 1 0.5811 8.1 0.885 Contact Map
3ppuA 2 1 7.7 0.886 Contact Map
4g0iA 2 1 7.3 0.887 Contact Map
4pfiA 1 0.527 6.8 0.889 Contact Map
1af7A 1 0.6892 6.4 0.89 Contact Map
1fxkA 2 0.9324 5.8 0.892 Contact Map
3le1A 1 0.3378 5.8 0.892 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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