GREMLIN Database
DUF2157 - Predicted membrane protein (DUF2157)
PFAM: PF09925 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 147 (141)
Sequences: 7989 (6160)
Seq/√Len: 518.7
META: 0.891

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
103_A134_A3.8691.00
10_S13_Q3.3771.00
6_A20_L3.2701.00
4_V14_A2.9761.00
70_L109_G2.8071.00
4_V11_A2.7301.00
62_G65_A2.5681.00
11_A15_E2.5551.00
107_G130_W2.1721.00
106_F133_L2.1301.00
15_E19_A2.0971.00
50_V105_L1.9551.00
2_R6_A1.9431.00
47_G104_L1.8751.00
82_A86_R1.8651.00
43_A97_A1.8461.00
77_A101_L1.7851.00
73_L105_L1.7671.00
80_A98_L1.7531.00
106_F134_A1.7351.00
31_R34_L1.7221.00
83_L86_R1.6731.00
51_I108_A1.6591.00
13_Q16_R1.6251.00
121_G126_L1.6161.00
14_A18_L1.5911.00
17_I20_L1.5881.00
99_L141_L1.5861.00
81_G102_G1.5561.00
79_A83_L1.5381.00
77_A105_L1.5241.00
26_S29_A1.4791.00
67_L113_I1.4541.00
8_L13_Q1.3881.00
78_Y102_G1.3841.00
61_L65_A1.3781.00
77_A102_G1.3611.00
124_P127_F1.3581.00
43_A101_L1.3461.00
12_E15_E1.3371.00
123_W127_F1.3321.00
115_Q118_H1.3271.00
16_R19_A1.3131.00
15_E18_L1.3051.00
36_R40_Y1.2751.00
9_I13_Q1.2721.00
47_G51_I1.2641.00
58_W66_R1.2511.00
74_L78_Y1.2351.00
51_I54_V1.2341.00
112_L116_I1.2201.00
57_N115_Q1.2071.00
67_L71_L1.2011.00
96_E143_S1.2011.00
123_W126_L1.1851.00
10_S16_R1.1681.00
114_G121_G1.1581.00
70_L73_L1.1571.00
74_L109_G1.1561.00
94_L98_L1.1361.00
66_R116_I1.1251.00
110_I126_L1.1181.00
63_R117_Y1.1081.00
12_E16_R1.0961.00
20_L24_R1.0851.00
64_L68_A1.0731.00
71_L109_G1.0721.00
93_A97_A1.0591.00
57_N61_L1.0581.00
111_A115_Q1.0481.00
128_L131_A1.0421.00
39_L97_A1.0401.00
137_L140_L1.0341.00
78_Y99_L1.0311.00
78_Y81_G1.0221.00
74_L77_A1.0211.00
60_E63_R1.0211.00
81_G141_L0.9971.00
3_W8_L0.9941.00
120_G125_D0.9741.00
2_R20_L0.9651.00
63_R116_I0.9561.00
84_L95_G0.9521.00
77_A98_L0.9421.00
65_A68_A0.9421.00
74_L105_L0.9051.00
17_I21_Y0.8961.00
122_D127_F0.8921.00
120_G123_W0.8851.00
124_P128_L0.8771.00
120_G124_P0.8771.00
4_V18_L0.8691.00
133_L136_P0.8651.00
11_A14_A0.8591.00
82_A85_R0.8561.00
98_L102_G0.8451.00
61_L73_L0.8431.00
81_G99_L0.8311.00
86_R89_R0.8301.00
13_Q17_I0.8281.00
108_A112_L0.8241.00
114_G120_G0.8221.00
54_V57_N0.8071.00
43_A47_G0.8041.00
52_L56_A0.8001.00
55_A115_Q0.7961.00
126_L130_W0.7921.00
125_D128_L0.7881.00
122_D125_D0.7831.00
140_L143_S0.7811.00
22_R25_P0.7761.00
37_I42_G0.7701.00
91_S94_L0.7651.00
39_L43_A0.7641.00
95_G102_G0.7631.00
125_D129_L0.7621.00
92_P96_E0.7611.00
68_A72_A0.7591.00
79_A82_A0.7571.00
9_I14_A0.7551.00
103_A137_L0.7551.00
135_L138_A0.7531.00
110_I121_G0.7461.00
8_L21_Y0.7441.00
50_V54_V0.7421.00
73_L77_A0.7141.00
126_L133_L0.7111.00
57_N60_E0.7061.00
23_A26_S0.7051.00
21_Y24_R0.7041.00
92_P95_G0.7031.00
109_G113_I0.7021.00
130_W134_A0.7011.00
35_L39_L0.6931.00
69_L72_A0.6891.00
114_G126_L0.6881.00
8_L20_L0.6871.00
52_L55_A0.6851.00
111_A114_G0.6801.00
19_A23_A0.6791.00
67_L109_G0.6771.00
118_H121_G0.6771.00
69_L73_L0.6691.00
22_R26_S0.6621.00
121_G125_D0.6601.00
85_R96_E0.6581.00
135_L139_Y0.6511.00
47_G101_L0.6491.00
80_A83_L0.6471.00
47_G97_A0.6411.00
104_L108_A0.6361.00
16_R20_L0.6311.00
121_G124_P0.6301.00
93_A96_E0.6291.00
3_W21_Y0.6261.00
57_N117_Y0.6241.00
70_L116_I0.6231.00
131_A135_L0.6171.00
54_V108_A0.6161.00
2_R5_E0.6111.00
129_L132_L0.6111.00
37_I41_L0.6091.00
48_A51_I0.6081.00
94_L97_A0.6061.00
24_R29_A0.6041.00
75_L79_A0.6041.00
122_D126_L0.6011.00
81_G95_G0.6001.00
18_L26_S0.5990.99
99_L103_A0.5980.99
6_A17_I0.5930.99
95_G99_L0.5900.99
128_L132_L0.5870.99
76_A80_A0.5800.99
49_G56_A0.5770.99
132_L136_P0.5760.99
136_P140_L0.5720.99
44_L100_L0.5700.99
70_L74_L0.5680.99
58_W115_Q0.5660.99
3_W9_I0.5570.99
22_R27_S0.5560.99
103_A107_G0.5550.99
66_R70_L0.5530.99
18_L22_R0.5520.99
80_A84_L0.5520.99
40_Y47_G0.5510.99
42_G46_L0.5510.99
4_V13_Q0.5500.99
85_R95_G0.5460.99
19_A22_R0.5430.99
76_A79_A0.5430.99
36_R101_L0.5420.99
61_L69_L0.5420.99
70_L112_L0.5410.99
106_F113_I0.5390.99
71_L113_I0.5360.99
26_S34_L0.5350.99
8_L16_R0.5230.99
40_Y44_L0.5180.99
119_L125_D0.5170.99
47_G108_A0.5150.98
111_A126_L0.5120.98
23_A33_R0.5080.98
3_W17_I0.5080.98
120_G127_F0.5070.98
114_G125_D0.5060.98
106_F109_G0.5050.98
99_L102_G0.5040.98
24_R32_R0.5030.98
78_Y137_L0.5020.98
100_L143_S0.5000.98
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4ev6A 3 0.5986 19.1 0.886 Contact Map
4pgrA 1 0.7687 16.4 0.89 Contact Map
4i0uA 4 0.585 14.4 0.892 Contact Map
2xf7A 3 0.1769 13.5 0.894 Contact Map
3pjzA 2 0.7891 12.6 0.895 Contact Map
2vakA 2 0.449 9.6 0.901 Contact Map
2nn4A 1 0.1905 8.1 0.904 Contact Map
4qinA 2 0.7551 8.1 0.904 Contact Map
4ikvA 1 0.8503 7 0.906 Contact Map
4bqqA 2 0.3333 6.3 0.909 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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