GREMLIN Database
DUF2145 - Uncharacterized protein conserved in bacteria (DUF2145)
PFAM: PF09916 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 200 (199)
Sequences: 1399 (1001)
Seq/√Len: 71.0
META: 0.847

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
182_D189_E5.3811.00
66_F75_A3.4741.00
117_Y176_D3.0831.00
136_A141_Q2.6491.00
101_L130_A2.5921.00
55_S102_H2.5611.00
24_L156_L2.5311.00
49_A56_R2.4281.00
21_R162_T2.4271.00
2_L10_A2.3911.00
180_N184_F2.3851.00
105_R117_Y2.3841.00
57_L99_L2.1061.00
84_L132_W2.1031.00
8_R89_L2.0821.00
81_P84_L2.0231.00
108_A173_V2.0211.00
138_T141_Q1.9941.00
175_F188_I1.9901.00
70_P73_Y1.9061.00
158_V162_T1.8631.00
117_Y174_A1.8511.00
129_A199_F1.8501.00
19_L27_S1.8311.00
159_G162_T1.8101.00
133_G148_A1.7851.00
25_R74_E1.7781.00
80_P84_L1.7761.00
24_L192_T1.7541.00
12_V43_V1.7241.00
45_H120_C1.7001.00
45_H60_Q1.7001.00
156_L192_T1.6971.00
13_A76_S1.6941.00
105_R116_R1.6821.00
155_T182_D1.6821.00
80_P85_Q1.6130.99
115_T126_E1.6120.99
160_P186_G1.6020.99
113_F153_P1.5950.99
91_A131_A1.5840.99
2_L5_S1.5750.99
98_A123_W1.5570.99
38_D42_R1.5560.99
42_R59_D1.5190.99
162_T165_G1.5080.99
60_Q64_G1.5060.99
137_T141_Q1.5040.99
47_Y120_C1.4980.99
107_S112_P1.4960.99
48_N172_N1.4370.99
155_T189_E1.4190.98
161_L165_G1.4060.98
29_A193_V1.4060.98
7_A85_Q1.4010.98
9_V89_L1.3900.98
83_E87_R1.3880.98
110_A114_S1.3850.98
135_T141_Q1.3520.98
141_Q144_A1.3480.98
36_S40_P1.3470.98
115_T130_A1.3370.98
29_A124_V1.3370.98
125_L196_I1.3260.98
9_V30_G1.3050.97
92_L127_T1.2920.97
181_E184_F1.2830.97
66_F70_P1.2780.97
133_G149_Q1.2600.97
18_D21_R1.2580.97
139_R145_W1.2270.96
107_S177_D1.2160.96
44_R98_A1.2030.96
89_L93_L1.1760.95
50_C61_G1.1710.95
167_R183_R1.1610.95
77_I104_P1.1600.95
181_E186_G1.1490.94
155_T187_R1.1390.94
15_A72_R1.1300.94
8_R86_A1.1140.93
31_F127_T1.1060.93
30_G43_V1.1040.93
33_L92_L1.0890.93
184_F188_I1.0880.93
100_R137_T1.0850.93
122_Q126_E1.0630.92
12_V75_A1.0600.92
3_D7_A1.0540.91
8_R85_Q1.0530.91
63_G110_A1.0440.91
165_G175_F1.0380.91
129_A142_A1.0350.91
135_T148_A1.0260.90
160_P165_G1.0240.90
167_R175_F1.0160.90
152_E155_T1.0100.90
79_V85_Q1.0080.89
54_R104_P0.9960.89
46_L119_N0.9950.89
133_G141_Q0.9840.88
97_R100_R0.9790.88
163_R167_R0.9770.88
13_A49_A0.9760.88
112_P177_D0.9730.88
49_A52_T0.9670.87
113_F146_L0.9660.87
66_F73_Y0.9640.87
31_F92_L0.9640.87
76_S197_E0.9630.87
94_D97_R0.9600.87
162_T166_A0.9410.86
195_S198_A0.9400.86
3_D34_R0.9360.86
113_F143_Q0.9340.86
78_V197_E0.9220.85
43_V62_L0.9140.84
109_V166_A0.9140.84
57_L180_N0.9120.84
139_R142_A0.9100.84
59_D199_F0.9040.84
45_H48_N0.9040.84
97_R130_A0.9030.84
95_S99_L0.9020.83
165_G184_F0.8880.83
34_R87_R0.8840.82
126_E139_R0.8830.82
117_Y177_D0.8800.82
78_V200_L0.8760.82
107_S178_H0.8740.82
102_H132_W0.8720.81
108_A122_Q0.8680.81
167_R173_V0.8600.80
77_I110_A0.8510.80
154_T198_A0.8490.80
116_R176_D0.8460.79
13_A29_A0.8400.79
163_R188_I0.8380.79
9_V32_A0.8350.78
110_A122_Q0.8340.78
184_F187_R0.8260.78
147_R181_E0.8250.78
35_D40_P0.8210.77
24_L154_T0.8160.77
55_S103_E0.8150.77
15_A19_L0.8130.77
157_R181_E0.8130.77
35_D38_D0.8080.76
84_L200_L0.8030.76
126_E142_A0.7950.75
183_R188_I0.7920.75
10_A32_A0.7860.74
90_A93_L0.7860.74
34_R41_W0.7800.74
80_P136_A0.7770.73
171_A174_A0.7750.73
122_Q139_R0.7710.73
15_A25_R0.7700.73
161_L186_G0.7650.72
90_A95_S0.7640.72
26_Y101_L0.7610.72
11_L36_S0.7610.72
64_G76_S0.7550.71
4_A9_V0.7520.71
9_V33_L0.7480.70
84_L128_L0.7450.70
142_A145_W0.7440.70
11_L124_V0.7430.70
159_G180_N0.7390.70
17_Q27_S0.7390.70
175_F183_R0.7360.69
126_E143_Q0.7350.69
95_S136_A0.7340.69
176_D188_I0.7340.69
91_A101_L0.7310.69
15_A76_S0.7310.69
36_S42_R0.7300.69
48_N120_C0.7250.68
31_F91_A0.7230.68
33_L59_D0.7230.68
34_R40_P0.7210.68
188_I195_S0.7180.67
91_A123_W0.7160.67
11_L31_F0.7140.67
153_P195_S0.7140.67
18_D71_F0.7060.66
136_A142_A0.7050.66
67_L96_P0.7030.66
105_R110_A0.7030.66
57_L133_G0.7030.66
111_Y122_Q0.7020.66
22_Y124_V0.7010.65
64_G119_N0.6980.65
76_S80_P0.6980.65
6_G30_G0.6970.65
91_A97_R0.6940.65
38_D59_D0.6920.64
2_L7_A0.6890.64
33_L40_P0.6810.63
130_A166_A0.6790.63
22_Y47_Y0.6790.63
166_A169_F0.6760.63
111_Y177_D0.6740.62
180_N186_G0.6740.62
46_L55_S0.6720.62
160_P164_L0.6630.61
87_R90_A0.6620.61
161_L184_F0.6590.61
20_S23_G0.6580.61
140_A147_R0.6550.60
53_A172_N0.6490.59
95_S98_A0.6480.59
157_R187_R0.6320.57
17_Q22_Y0.6310.57
88_L115_T0.6290.57
60_Q98_A0.6280.57
182_D188_I0.6280.57
111_Y145_W0.6270.57
152_E198_A0.6270.57
22_Y27_S0.6240.57
164_L188_I0.6240.57
135_T138_T0.6230.56
88_L99_L0.6210.56
15_A71_F0.6170.56
156_L166_A0.6160.56
107_S118_Q0.6110.55
22_Y120_C0.6050.54
124_V127_T0.6050.54
193_V197_E0.6040.54
35_D77_I0.6030.54
112_P132_W0.6030.54
43_V129_A0.5950.53
105_R112_P0.5940.53
9_V92_L0.5940.53
83_E144_A0.5910.53
70_P75_A0.5900.52
135_T147_R0.5880.52
81_P200_L0.5870.52
109_V158_V0.5860.52
19_L25_R0.5840.52
84_L101_L0.5810.51
57_L101_L0.5810.51
164_L185_A0.5790.51
128_L196_I0.5780.51
43_V199_F0.5780.51
32_A163_R0.5770.51
162_T172_N0.5760.51
165_G186_G0.5750.51
7_A79_V0.5720.50
86_A149_Q0.5720.50
144_A148_A0.5700.50
168_M184_F0.5700.50
125_L146_L0.5670.50
24_L68_D0.5670.50
36_S39_G0.5640.49
10_A31_F0.5630.49
64_G95_S0.5620.49
80_P102_H0.5620.49
24_L153_P0.5620.49
42_R61_G0.5610.49
71_F77_I0.5610.49
143_Q178_H0.5610.49
158_V188_I0.5610.49
141_Q148_A0.5590.49
111_Y179_P0.5570.48
116_R137_T0.5570.48
19_L129_A0.5550.48
114_S180_N0.5540.48
194_D199_F0.5520.48
31_F124_V0.5480.47
113_F119_N0.5460.47
12_V17_Q0.5440.47
121_N129_A0.5430.47
25_R69_D0.5430.47
40_P44_R0.5400.46
192_T195_S0.5400.46
4_A77_I0.5390.46
182_D186_G0.5390.46
87_R95_S0.5380.46
141_Q145_W0.5360.46
168_M186_G0.5360.46
89_L196_I0.5330.45
92_L95_S0.5320.45
164_L167_R0.5290.45
107_S117_Y0.5270.45
165_G168_M0.5260.45
161_L164_L0.5260.45
170_V173_V0.5260.45
30_G124_V0.5210.44
15_A37_P0.5210.44
15_A18_D0.5180.44
69_D101_L0.5170.44
87_R181_E0.5140.43
34_R77_I0.5110.43
132_W135_T0.5100.43
59_D122_Q0.5070.42
139_R143_Q0.5040.42
24_L27_S0.5000.41
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3kw0A 3 0.72 75.9 0.941 Contact Map
2if6A 1 0.78 72.7 0.942 Contact Map
4q68A 1 0.355 67.7 0.944 Contact Map
2p1gA 1 0.345 41.3 0.952 Contact Map
2im9A 1 0.41 38.5 0.953 Contact Map
3jrnA 1 0.645 15.7 0.961 Contact Map
4mtdA 2 0.33 15.4 0.961 Contact Map
4dotA 1 0.48 14.7 0.962 Contact Map
2xigA 4 0.34 14.2 0.962 Contact Map
4rifA 2 0.625 12.6 0.963 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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