GREMLIN Database
DUF2140 - Uncharacterized protein conserved in bacteria (DUF2140)
PFAM: PF09911 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 187 (186)
Sequences: 597 (466)
Seq/√Len: 34.2
META: 0.219

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
175_D178_N6.6191.00
173_K180_D4.8831.00
57_N61_N4.6141.00
112_L174_I3.8901.00
172_K184_E3.5721.00
111_N159_T3.1281.00
176_L181_I3.0541.00
11_L14_N2.9791.00
88_T95_E2.8601.00
148_N151_E2.7971.00
2_K6_A2.4491.00
8_L12_A2.3500.99
121_V124_L2.2620.99
49_V185_I2.1970.99
77_V83_V2.0780.99
3_W10_L1.8600.97
1_N4_K1.8530.97
7_F10_L1.8410.97
111_N157_D1.8230.97
7_F11_L1.7830.96
61_N85_L1.6900.95
1_N14_N1.6770.94
75_Y145_V1.6750.94
10_L175_D1.6360.94
3_W7_F1.6340.94
45_A184_E1.6210.93
148_N153_T1.5520.92
110_G127_P1.5300.91
132_L141_L1.5280.91
53_K79_L1.5240.91
104_P110_G1.5140.91
117_K160_E1.4940.90
6_A136_K1.4800.90
110_G114_L1.4760.90
113_V155_Y1.4350.88
60_I185_I1.4120.87
128_V152_E1.4020.87
16_L20_V1.3950.87
72_P150_K1.3930.87
57_N75_Y1.3600.85
12_A160_E1.3550.85
51_T92_F1.3500.85
1_N5_W1.3500.85
79_L181_I1.3170.83
87_G139_Y1.3020.83
52_T147_I1.2910.82
119_I128_V1.2880.82
172_K182_S1.2720.81
75_Y139_Y1.2650.81
87_G98_F1.2600.81
52_T55_Q1.2510.80
12_A157_D1.2350.79
108_E160_E1.2250.79
53_K179_D1.2240.79
6_A111_N1.2170.78
128_V185_I1.1900.77
112_L171_A1.1770.76
103_E107_L1.1680.75
75_Y119_I1.1650.75
103_E117_K1.1650.75
17_V21_L1.1540.74
91_V145_V1.1520.74
73_I102_F1.1510.74
63_Y139_Y1.1510.74
88_T119_I1.1430.74
61_N119_I1.1390.73
14_N17_V1.1240.72
113_V153_T1.1230.72
125_P151_E1.1200.72
23_I26_F1.1120.72
2_K5_W1.1050.71
63_Y134_Y1.1030.71
82_D118_S1.1020.71
116_Q128_V1.0930.70
162_K168_K1.0870.70
135_I139_Y1.0860.70
56_L119_I1.0810.69
132_L185_I1.0800.69
107_L111_N1.0590.68
6_A14_N1.0540.67
60_I160_E1.0540.67
29_S99_S1.0530.67
83_V109_N1.0450.67
103_E106_V1.0430.67
56_L183_F1.0370.66
116_Q155_Y1.0230.65
40_S78_Y1.0180.65
6_A98_F1.0070.64
42_E96_V1.0040.64
158_L171_A0.9990.63
20_V23_I0.9980.63
156_V174_I0.9950.63
171_A183_F0.9920.63
12_A16_L0.9900.62
147_I185_I0.9710.61
120_S145_V0.9710.61
99_S120_S0.9600.60
91_V107_L0.9510.59
100_L116_Q0.9430.59
19_I22_F0.9360.58
128_V174_I0.9360.58
14_N98_F0.9280.57
86_Y96_V0.9220.57
110_G142_P0.9210.57
108_E132_L0.9170.56
115_K153_T0.9090.56
149_P155_Y0.9080.56
5_W8_L0.9060.56
161_I168_K0.9050.55
98_F102_F0.8930.54
79_L117_K0.8890.54
82_D117_K0.8870.54
3_W6_A0.8860.54
25_I133_K0.8830.54
75_Y134_Y0.8790.53
121_V126_L0.8750.53
143_E161_I0.8650.52
116_Q144_W0.8650.52
6_A141_L0.8610.52
131_V139_Y0.8590.52
19_I23_I0.8570.51
9_I17_V0.8560.51
53_K106_V0.8540.51
64_L185_I0.8510.51
105_E117_K0.8470.51
134_Y145_V0.8470.51
110_G144_W0.8400.50
105_E115_K0.8320.49
45_A62_Y0.8280.49
105_E113_V0.8260.49
80_D178_N0.8230.49
137_K150_K0.8200.48
82_D101_T0.8130.48
60_I77_V0.8120.48
143_E160_E0.8110.48
33_E96_V0.8110.48
156_V163_L0.8050.47
57_N78_Y0.7980.46
55_Q67_E0.7930.46
128_V132_L0.7890.46
85_L141_L0.7880.46
47_F185_I0.7880.46
11_L20_V0.7870.46
130_Y133_K0.7850.45
11_L118_S0.7820.45
87_G154_I0.7780.45
51_T98_F0.7760.45
105_E135_I0.7740.44
146_T157_D0.7740.44
77_V84_E0.7730.44
102_F142_P0.7630.43
31_D54_E0.7590.43
5_W58_A0.7490.42
161_I164_K0.7470.42
146_T160_E0.7470.42
13_L24_L0.7420.42
5_W9_I0.7420.42
85_L137_K0.7370.41
16_L91_V0.7370.41
74_D87_G0.7300.41
25_I152_E0.7290.41
132_L152_E0.7290.41
59_L119_I0.7280.41
47_F128_V0.7250.40
51_T89_L0.7250.40
46_V184_E0.7210.40
108_E125_P0.7210.40
15_L143_E0.7160.40
73_I180_D0.7160.40
22_F173_K0.7140.39
35_P88_T0.7130.39
51_T55_Q0.7110.39
83_V121_V0.7100.39
8_L74_D0.7100.39
64_L70_D0.7070.39
9_I36_P0.7040.39
79_L83_V0.7020.38
134_Y139_Y0.6980.38
52_T128_V0.6970.38
104_P142_P0.6940.38
80_D125_P0.6940.38
10_L50_S0.6940.38
19_I80_D0.6940.38
129_S147_I0.6900.38
87_G120_S0.6890.37
12_A25_I0.6880.37
102_F114_L0.6870.37
6_A115_K0.6790.37
55_Q63_Y0.6790.37
61_N141_L0.6760.36
11_L34_L0.6750.36
76_K175_D0.6740.36
141_L154_I0.6720.36
64_L77_V0.6720.36
18_I151_E0.6710.36
9_I136_K0.6690.36
89_L175_D0.6690.36
92_F130_Y0.6680.36
9_I15_L0.6650.36
89_L98_F0.6640.35
90_K95_E0.6630.35
20_V24_L0.6600.35
131_V138_S0.6590.35
96_V137_K0.6560.35
102_F149_P0.6550.35
73_I117_K0.6420.34
170_K186_Y0.6410.34
149_P169_V0.6410.34
9_I49_V0.6380.34
165_S168_K0.6350.33
67_E72_P0.6350.33
171_A181_I0.6330.33
162_K170_K0.6290.33
73_I89_L0.6290.33
43_E48_T0.6270.33
83_V149_P0.6160.32
123_T126_L0.6140.32
81_D172_K0.6120.32
45_A49_V0.6110.31
142_P171_A0.6090.31
48_T131_V0.6060.31
87_G145_V0.6050.31
9_I13_L0.6010.31
59_L79_L0.6000.31
162_K166_G0.5980.31
76_K133_K0.5960.30
169_V179_D0.5910.30
112_L134_Y0.5910.30
54_E125_P0.5860.30
91_V106_V0.5830.29
62_Y121_V0.5810.29
20_V117_K0.5740.29
55_Q92_F0.5720.29
17_V23_I0.5720.29
6_A64_L0.5710.29
5_W24_L0.5680.28
60_I79_L0.5640.28
90_K100_L0.5620.28
55_Q116_Q0.5590.28
15_L26_F0.5590.28
176_L179_D0.5550.28
49_V59_L0.5540.27
16_L109_N0.5540.27
69_K74_D0.5540.27
135_I184_E0.5500.27
59_L101_T0.5480.27
58_A62_Y0.5460.27
126_L142_P0.5440.27
90_K119_I0.5400.27
86_Y98_F0.5400.27
49_V89_L0.5400.27
15_L23_I0.5390.26
59_L137_K0.5370.26
97_P119_I0.5360.26
18_I23_I0.5350.26
67_E70_D0.5340.26
39_K177_K0.5320.26
42_E76_K0.5310.26
56_L161_I0.5300.26
47_F51_T0.5270.26
66_D137_K0.5260.26
47_F126_L0.5240.26
62_Y147_I0.5240.26
146_T179_D0.5220.25
13_L23_I0.5210.25
70_D108_E0.5210.25
56_L61_N0.5180.25
41_T50_S0.5160.25
22_F93_G0.5120.25
172_K185_I0.5120.25
9_I160_E0.5090.25
5_W12_A0.5070.24
87_G117_K0.5070.24
45_A72_P0.5060.24
172_K177_K0.5030.24
11_L175_D0.5000.24
149_P176_L0.5000.24
144_W158_L0.5000.24
29_S132_L0.5000.24
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2xtcA 4 0.3316 9.5 0.949 Contact Map
1q8kA 1 0.3904 8.8 0.95 Contact Map
4tr8A 2 0.7005 7.8 0.951 Contact Map
5agvA 3 0.6257 7.8 0.951 Contact Map
1wi5A 1 0.4278 7.5 0.952 Contact Map
4tphA 2 0.1711 7.4 0.952 Contact Map
4trtA 2 0.6203 6.7 0.953 Contact Map
4nngA 1 0.4011 6.2 0.953 Contact Map
2cqaA 1 0.508 5.8 0.954 Contact Map
3aevA 1 0.3476 5.3 0.955 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0071 seconds.