GREMLIN Database
DUF2058 - Uncharacterized protein conserved in bacteria (DUF2058)
PFAM: PF09831 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 174 (174)
Sequences: 3340 (2216)
Seq/√Len: 168.0
META: 0.83

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
56_E59_R2.9891.00
96_D122_R2.8521.00
82_R128_E2.7071.00
62_Q66_E2.5821.00
96_D127_Y2.5691.00
91_A102_R2.4841.00
59_R63_E2.3831.00
111_K115_A2.3431.00
45_R49_E2.3001.00
85_R128_E2.1181.00
91_A104_Y2.0481.00
44_Q47_Q2.0251.00
13_D16_K2.0211.00
105_V113_L1.9731.00
52_E56_E1.9431.00
65_A69_A1.8931.00
132_A136_E1.8771.00
109_Q129_L1.8221.00
94_F127_Y1.7921.00
83_L118_L1.7891.00
66_E69_A1.7721.00
56_E60_Q1.7611.00
60_Q63_E1.7601.00
90_I110_R1.7301.00
50_K54_D1.7241.00
44_Q48_A1.7171.00
136_E140_E1.6781.00
112_Q117_R1.6661.00
48_A52_E1.6531.00
22_K25_R1.6381.00
107_A111_K1.6321.00
47_Q51_A1.5931.00
52_E55_R1.5891.00
37_D40_K1.5501.00
121_V130_V1.5431.00
118_L129_L1.5421.00
136_E139_R1.5221.00
63_E67_R1.4701.00
62_Q65_A1.4661.00
106_T109_Q1.4581.00
93_N102_R1.4551.00
121_V146_V1.4531.00
66_E70_I1.4501.00
73_Q141_R1.4421.00
41_A44_Q1.4311.00
59_R62_Q1.4281.00
92_Y148_L1.4261.00
119_A135_A1.4241.00
14_K18_K1.4161.00
69_A73_Q1.4111.00
77_L134_V1.4011.00
53_R57_L1.3851.00
12_V16_K1.3791.00
108_E112_Q1.3771.00
48_A51_A1.3621.00
75_K124_D1.3451.00
39_A43_A1.3381.00
110_R114_S1.3081.00
63_E66_E1.2981.00
130_V134_V1.2941.00
159_E162_P1.2881.00
41_A45_R1.2811.00
109_Q118_L1.2771.00
164_A167_Q1.2701.00
28_R32_R1.2631.00
3_Q21_K1.2541.00
74_I142_D1.2291.00
16_K20_A1.2251.00
81_N134_V1.2211.00
1_S4_D1.2211.00
46_A50_K1.2191.00
55_R59_R1.2161.00
40_K44_Q1.1981.00
12_V17_A1.1821.00
15_K22_K1.1791.00
42_A45_R1.1691.00
60_Q64_A1.1661.00
74_I145_A1.1621.00
95_T98_G1.1481.00
47_Q50_K1.1431.00
136_E149_L1.1371.00
157_P162_P1.1311.00
43_A46_A1.1211.00
76_Q79_E1.1161.00
58_N62_Q1.1041.00
3_Q7_L1.0941.00
162_P165_D1.0891.00
119_A149_L1.0791.00
7_L14_K1.0751.00
75_K79_E1.0701.00
58_N61_R1.0701.00
2_L6_L1.0681.00
17_A20_A1.0500.99
45_R48_A1.0390.99
90_I107_A1.0350.99
158_D162_P1.0340.99
107_A110_R1.0270.99
139_R149_L1.0210.99
88_G106_T1.0190.99
16_K19_K1.0160.99
156_A159_E1.0120.99
122_R127_Y0.9900.99
54_D57_L0.9780.99
35_E38_E0.9600.99
74_I138_I0.9480.99
64_A67_R0.9370.99
78_I123_L0.9340.99
57_L61_R0.9250.99
130_V138_I0.9250.99
139_R143_P0.9240.99
21_K25_R0.9220.99
78_I121_V0.9220.99
171_D174_W0.9100.98
51_A55_R0.9010.98
39_A42_A0.8970.98
157_P167_Q0.8970.98
38_E41_A0.8930.98
38_E42_A0.8900.98
77_L80_Q0.8790.98
30_Q33_K0.8710.98
29_K32_R0.8680.98
92_Y114_S0.8650.98
72_A141_R0.8640.98
36_V41_A0.8590.98
71_A75_K0.8590.98
79_E123_L0.8520.98
22_K26_K0.8400.97
155_E160_D0.8380.97
89_D107_A0.8370.97
151_D154_E0.8330.97
66_E73_Q0.8310.97
14_K17_A0.8260.97
137_K140_E0.8260.97
12_V20_A0.8180.97
25_R32_R0.8120.97
103_I109_Q0.8120.97
61_R64_A0.8090.97
73_Q77_L0.8070.97
51_A54_D0.8020.96
121_V128_E0.8010.96
131_P134_V0.8000.96
108_E111_K0.8000.96
58_N69_A0.7980.96
123_L126_R0.7900.96
37_D41_A0.7680.96
162_P166_Y0.7510.95
133_E136_E0.7450.95
155_E158_D0.7430.95
78_I138_I0.7420.95
95_T100_I0.7390.94
18_K21_K0.7360.94
122_R147_V0.7350.94
78_I122_R0.7320.94
163_Y166_Y0.7310.94
23_E26_K0.7280.94
31_K34_K0.7270.94
73_Q137_K0.7220.94
49_E52_E0.7190.94
71_A142_D0.7190.94
132_A149_L0.7190.94
119_A130_V0.7110.93
85_R109_Q0.7070.93
46_A49_E0.7020.93
134_V141_R0.7000.93
29_K33_K0.6970.93
169_P174_W0.6950.92
86_D109_Q0.6890.92
71_A124_D0.6880.92
15_K19_K0.6870.92
119_A132_A0.6870.92
18_K22_K0.6860.92
161_D164_A0.6840.92
25_R29_K0.6830.92
153_A156_A0.6820.92
103_I129_L0.6800.92
19_K29_K0.6770.91
84_P87_D0.6770.91
158_D161_D0.6740.91
170_D174_W0.6740.91
109_Q112_Q0.6720.91
105_V109_Q0.6710.91
120_I147_V0.6690.91
79_E82_R0.6680.91
146_V149_L0.6620.91
105_V110_R0.6580.90
100_I150_N0.6530.90
3_Q17_A0.6520.90
130_V135_A0.6510.90
152_A157_P0.6510.90
30_Q34_K0.6490.90
59_R82_R0.6450.89
153_A160_D0.6440.89
42_A46_A0.6440.89
162_P167_Q0.6430.89
67_R71_A0.6410.89
130_V146_V0.6400.89
7_L12_V0.6390.89
88_G113_L0.6390.89
119_A148_L0.6380.89
24_K32_R0.6350.89
26_K30_Q0.6340.89
133_E137_K0.6340.89
24_K29_K0.6300.88
71_A78_I0.6250.88
15_K25_R0.6160.87
13_D20_A0.6130.87
86_D106_T0.6120.87
27_Q31_K0.6100.87
77_L138_I0.6080.86
57_L74_I0.6020.86
138_I141_R0.6010.86
113_L148_L0.5940.85
12_V92_Y0.5910.85
9_A13_D0.5860.84
102_R110_R0.5790.84
99_K125_G0.5740.83
154_E157_P0.5720.83
8_K12_V0.5690.83
90_I105_V0.5660.82
88_G109_Q0.5650.82
51_A141_R0.5600.82
3_Q24_K0.5580.81
142_D145_A0.5560.81
100_I110_R0.5540.81
83_L129_L0.5520.81
9_A51_A0.5510.81
157_P160_D0.5480.80
76_Q81_N0.5470.80
21_K27_Q0.5470.80
4_D28_R0.5460.80
23_E27_Q0.5450.80
152_A155_E0.5430.80
9_A17_A0.5430.80
169_P172_L0.5420.80
70_I74_I0.5420.80
25_R93_N0.5410.79
111_K114_S0.5380.79
19_K26_K0.5370.79
95_T148_L0.5330.78
70_I73_Q0.5290.78
21_K32_R0.5240.77
49_E90_I0.5230.77
49_E53_R0.5220.77
1_S17_A0.5210.77
64_A68_K0.5180.76
43_A47_Q0.5170.76
11_L14_K0.5130.76
3_Q9_A0.5130.76
20_A23_E0.5120.76
25_R28_R0.5120.76
83_L109_Q0.5120.76
165_D173_M0.5110.76
77_L140_E0.5110.76
15_K29_K0.5100.75
81_N135_A0.5080.75
54_D58_N0.5080.75
1_S5_Q0.5040.75
162_P173_M0.5040.75
161_D165_D0.5030.74
61_R65_A0.5010.74
85_R88_G0.5000.74
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1pkhA 5 0.3851 12.1 0.948 Contact Map
4xk8G 1 0.3391 8.2 0.952 Contact Map
4rb5B 1 0.5805 5.4 0.956 Contact Map
1kcaA 6 0.3621 4.8 0.957 Contact Map
4relA 1 0.3621 4.8 0.957 Contact Map
1w85A 2 0.4023 4.3 0.958 Contact Map
2v3sA 1 0.5 4 0.958 Contact Map
3t5xB 1 0 3.5 0.959 Contact Map
3ojaB 1 0.4713 3.2 0.96 Contact Map
1vi6A 2 0.6207 3.1 0.96 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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