GREMLIN Database
DUF2391 - Putative integral membrane protein (DUF2391)
PFAM: PF09622 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 268 (265)
Sequences: 570 (468)
Seq/√Len: 28.7
META: 0.725

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
230_Y234_T4.9851.00
229_A238_L4.1101.00
178_L182_A3.7201.00
5_G75_I3.6491.00
41_T111_S3.4591.00
6_F79_A3.3081.00
156_A257_A3.2821.00
89_V171_K3.2301.00
225_L254_G3.2021.00
148_M220_G3.1831.00
149_A223_V3.1321.00
81_A103_I3.0961.00
224_A257_A2.8871.00
159_V165_M2.7871.00
108_V157_F2.6751.00
87_L168_I2.6431.00
149_A227_V2.5600.99
163_E237_R2.4200.99
254_G257_A2.3600.99
49_S65_D2.2380.98
120_Q158_N2.2270.98
238_L246_W2.1450.98
35_L178_L2.0920.97
32_L35_L2.0400.97
192_V262_A1.9820.96
86_L179_A1.9680.96
218_L229_A1.9550.96
24_W27_F1.9330.96
86_L183_L1.9320.96
177_L248_S1.9030.95
73_L107_A1.8930.95
26_G101_G1.8680.95
88_G176_H1.7990.94
116_L150_A1.7960.94
49_S59_T1.7880.94
94_M98_E1.7630.93
109_P157_F1.7520.93
16_L156_A1.7460.93
75_I114_A1.7380.93
20_M24_W1.7120.92
252_V256_P1.6980.92
36_L40_L1.6960.92
44_L157_F1.6820.91
181_I212_A1.6610.91
59_T65_D1.6540.91
179_A183_L1.6210.90
98_E102_K1.6040.89
94_M99_I1.5870.89
143_G147_L1.5710.88
29_M47_G1.5560.88
67_L156_A1.5510.88
22_M165_M1.5470.87
261_A264_A1.5170.86
78_V153_L1.5160.86
30_D35_L1.5090.86
82_L85_L1.4930.86
29_M105_L1.4890.85
196_G268_L1.4640.84
229_A250_T1.4590.84
105_L116_L1.4480.84
55_R59_T1.4470.84
158_N266_L1.4400.83
16_L22_M1.4400.83
75_I173_T1.4280.83
119_S268_L1.3520.79
42_F70_V1.3510.79
176_H179_A1.3270.78
173_T176_H1.3110.77
144_E192_V1.2910.76
12_F15_P1.2810.75
163_E167_L1.2760.75
29_M234_T1.2710.75
103_I169_A1.2680.75
59_T80_S1.2630.74
60_A64_D1.2580.74
263_A268_L1.2480.73
54_F72_A1.2470.73
44_L149_A1.2350.73
155_L222_A1.2190.72
45_L115_L1.2080.71
4_R8_G1.2040.71
94_M102_K1.1980.70
163_E166_I1.1930.70
34_L112_I1.1780.69
148_M216_F1.1760.69
2_L54_F1.1670.68
192_V217_T1.1570.68
103_I178_L1.1550.67
119_S188_M1.1540.67
140_G147_L1.1520.67
159_V163_E1.1510.67
169_A248_S1.1470.67
64_D172_M1.1390.66
147_L195_V1.1340.66
40_L198_R1.1270.65
56_R130_E1.1260.65
118_R147_L1.1230.65
181_I244_T1.1140.65
128_D131_E1.1110.64
236_G239_D1.1030.64
19_T23_W1.1020.64
256_P265_R1.0940.63
263_A267_I1.0900.63
74_A77_I1.0880.63
184_S224_A1.0860.63
98_E101_G1.0820.62
75_I117_A1.0790.62
241_D249_A1.0740.62
222_A236_G1.0720.62
153_L169_A1.0690.61
95_P100_V1.0670.61
211_Q215_R1.0660.61
50_R60_A1.0640.61
77_I107_A1.0580.60
69_A237_R1.0550.60
191_I267_I1.0470.60
14_L259_I1.0460.60
131_E134_E1.0430.59
81_A173_T1.0420.59
46_V69_A1.0310.58
55_R67_L1.0300.58
176_H181_I1.0290.58
5_G114_A1.0280.58
239_D261_A1.0200.58
142_L186_L1.0190.58
38_L182_A1.0140.57
229_A246_W1.0050.56
231_M236_G1.0030.56
61_S64_D1.0000.56
262_A266_L0.9930.56
161_P197_F0.9900.55
27_F102_K0.9870.55
262_A265_R0.9810.55
68_D180_L0.9780.54
50_R119_S0.9760.54
155_L188_M0.9730.54
41_T73_L0.9700.54
104_A228_S0.9670.54
179_A182_A0.9650.53
256_P262_A0.9650.53
264_A267_I0.9570.53
10_L37_L0.9570.53
11_L106_Q0.9550.53
152_A257_A0.9540.53
189_H192_V0.9530.52
236_G242_P0.9530.52
216_F220_G0.9500.52
260_G265_R0.9490.52
61_S169_A0.9430.52
44_L112_I0.9400.51
14_L251_V0.9250.50
32_L142_L0.9160.50
168_I171_K0.9140.49
69_A112_I0.9140.49
5_G11_L0.9090.49
2_L5_G0.9090.49
77_I104_A0.8930.48
182_A186_L0.8870.47
5_G70_V0.8860.47
117_A200_Q0.8860.47
5_G8_G0.8830.47
139_A143_G0.8830.47
226_L246_W0.8820.47
44_L90_I0.8790.47
46_V66_V0.8790.47
190_A195_V0.8780.47
37_L147_L0.8740.46
62_W67_L0.8720.46
69_A111_S0.8680.46
224_A228_S0.8680.46
132_E169_A0.8550.45
78_V124_G0.8500.44
91_T258_A0.8490.44
86_L168_I0.8470.44
214_L240_G0.8460.44
36_L234_T0.8430.44
5_G9_A0.8400.44
11_L155_L0.8380.44
203_R212_A0.8360.43
67_L71_E0.8280.43
85_L176_H0.8270.43
16_L200_Q0.8270.43
133_G226_L0.8210.42
77_I139_A0.8190.42
133_G223_V0.8150.42
241_D251_V0.8140.42
112_I150_A0.8140.42
156_A228_S0.8130.42
13_G118_R0.8120.42
16_L247_L0.8110.42
29_M36_L0.8100.41
68_D120_Q0.8090.41
111_S173_T0.8070.41
173_T182_A0.8020.41
76_G231_M0.8000.41
209_L212_A0.7890.40
100_V243_L0.7860.40
208_G211_Q0.7850.40
215_R268_L0.7850.40
92_P187_L0.7800.39
119_S263_A0.7780.39
191_I268_L0.7760.39
233_W266_L0.7740.39
188_M222_A0.7730.39
265_R268_L0.7720.39
226_L229_A0.7720.39
29_M118_R0.7690.38
68_D121_L0.7680.38
64_D231_M0.7660.38
62_W254_G0.7560.37
13_G24_W0.7530.37
99_I268_L0.7530.37
9_A110_A0.7500.37
2_L13_G0.7470.37
216_F264_A0.7420.36
130_E134_E0.7410.36
175_W179_A0.7400.36
191_I263_A0.7400.36
12_F256_P0.7390.36
47_G209_L0.7380.36
37_L119_S0.7380.36
103_I244_T0.7360.36
81_A174_P0.7340.36
191_I227_V0.7340.36
119_S198_R0.7340.36
70_V75_I0.7330.36
193_Y251_V0.7310.36
49_S171_K0.7270.35
144_E216_F0.7260.35
36_L230_Y0.7240.35
43_P77_I0.7220.35
55_R115_L0.7200.35
233_W237_R0.7180.35
74_A246_W0.7150.35
168_I180_L0.7120.34
42_F87_L0.7080.34
3_A130_E0.7080.34
30_D33_R0.7060.34
115_L168_I0.7020.34
52_A257_A0.7010.34
66_V208_G0.7000.33
66_V130_E0.6990.33
15_P19_T0.6990.33
55_R119_S0.6960.33
208_G213_F0.6950.33
104_A166_I0.6950.33
96_L121_L0.6940.33
165_M170_Y0.6940.33
40_L93_D0.6930.33
188_M220_G0.6930.33
64_D77_I0.6920.33
222_A247_L0.6910.33
85_L179_A0.6910.33
5_G49_S0.6880.33
77_I117_A0.6870.33
85_L242_P0.6840.32
116_L154_F0.6800.32
193_Y196_G0.6780.32
43_P215_R0.6750.32
38_L163_E0.6690.31
166_I203_R0.6670.31
148_M261_A0.6660.31
144_E188_M0.6660.31
5_G63_R0.6650.31
36_L214_L0.6620.31
115_L150_A0.6600.31
33_R239_D0.6590.31
189_H213_F0.6580.31
47_G52_A0.6540.30
41_T61_S0.6530.30
241_D246_W0.6530.30
109_P192_V0.6530.30
41_T108_V0.6510.30
138_E250_T0.6480.30
244_T251_V0.6470.30
42_F46_V0.6470.30
49_S153_L0.6470.30
165_M252_V0.6460.30
22_M105_L0.6450.30
93_D172_M0.6430.30
122_G198_R0.6420.30
232_L235_F0.6410.30
48_L74_A0.6390.29
110_A148_M0.6380.29
140_G159_V0.6340.29
105_L144_E0.6340.29
10_L157_F0.6320.29
76_G80_S0.6290.29
196_G200_Q0.6290.29
128_D226_L0.6260.29
144_E148_M0.6250.28
8_G21_E0.6240.28
78_V179_A0.6230.28
11_L38_L0.6210.28
144_E158_N0.6210.28
199_G258_A0.6180.28
178_L227_V0.6180.28
119_S267_I0.6170.28
45_L51_Y0.6170.28
112_I248_S0.6150.28
107_A240_G0.6140.28
20_M23_W0.6130.28
12_F252_V0.6130.28
56_R182_A0.6100.28
243_L246_W0.6100.28
213_F216_F0.6100.28
107_A197_F0.6100.28
35_L41_T0.6100.28
82_L130_E0.6090.27
13_G110_A0.6090.27
165_M257_A0.6090.27
9_A148_M0.6090.27
90_I132_E0.6060.27
89_V193_Y0.6030.27
135_R229_A0.6030.27
89_V102_K0.6010.27
91_T129_D0.5990.27
116_L120_Q0.5990.27
29_M149_A0.5970.27
146_F228_S0.5960.27
135_R168_I0.5950.27
100_V214_L0.5930.26
7_A194_A0.5920.26
27_F235_F0.5920.26
29_M34_L0.5860.26
71_E263_A0.5860.26
46_V62_W0.5830.26
169_A257_A0.5830.26
100_V244_T0.5810.26
74_A197_F0.5800.26
172_M252_V0.5800.26
96_L100_V0.5800.26
21_E164_E0.5800.26
166_I202_E0.5790.26
73_L145_L0.5760.25
259_I268_L0.5750.25
144_E261_A0.5710.25
183_L187_L0.5680.25
170_Y251_V0.5600.25
16_L208_G0.5600.25
157_F257_A0.5590.24
142_L250_T0.5590.24
168_I202_E0.5580.24
82_L92_P0.5580.24
46_V83_V0.5550.24
217_T265_R0.5540.24
24_W102_K0.5540.24
42_F71_E0.5540.24
191_I238_L0.5520.24
34_L105_L0.5510.24
52_A150_A0.5500.24
86_L90_I0.5500.24
76_G92_P0.5490.24
93_D259_I0.5460.24
78_V187_L0.5430.24
92_P202_E0.5410.23
12_F155_L0.5400.23
106_Q155_L0.5400.23
158_N175_W0.5400.23
200_Q262_A0.5390.23
51_Y139_A0.5390.23
18_M189_H0.5380.23
96_L187_L0.5370.23
247_L250_T0.5300.23
183_L255_F0.5300.23
34_L198_R0.5280.23
15_P26_G0.5260.23
22_M76_G0.5260.23
192_V216_F0.5260.23
188_M268_L0.5250.23
22_M187_L0.5240.23
261_A267_I0.5240.23
103_I185_L0.5230.22
130_E226_L0.5230.22
179_A202_E0.5230.22
119_S191_I0.5230.22
80_S110_A0.5220.22
140_G143_G0.5210.22
101_G225_L0.5210.22
24_W28_Y0.5210.22
9_A114_A0.5190.22
2_L9_A0.5190.22
111_S119_S0.5180.22
32_L231_M0.5180.22
40_L124_G0.5150.22
149_A186_L0.5140.22
91_T175_W0.5130.22
18_M264_A0.5120.22
166_I237_R0.5110.22
216_F261_A0.5110.22
228_S253_L0.5090.22
49_S55_R0.5070.22
179_A231_M0.5050.22
26_G31_P0.5040.21
24_W171_K0.5040.21
29_M66_V0.5030.21
183_L242_P0.5000.21
37_L44_L0.5000.21
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1nkzA 4 0.1978 8.7 0.974 Contact Map
3wmmA 3 0.2164 5.8 0.976 Contact Map
3aqpA 1 0.4403 4.7 0.977 Contact Map
3zuxA 1 0.4104 4.6 0.977 Contact Map
4px7A 1 0.3955 4.2 0.977 Contact Map
4dx5A 3 0.4813 3.7 0.978 Contact Map
4jc95 3 0.1567 3.2 0.978 Contact Map
3w9iA 3 0.4515 2.6 0.979 Contact Map
1xrdA 1 0.194 2.4 0.98 Contact Map
2m3eA 1 0.1754 1.9 0.981 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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