GREMLIN Database
Cas_Csy3 - CRISPR-associated protein (Cas_Csy3)
PFAM: PF09615 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 327 (326)
Sequences: 938 (663)
Seq/√Len: 36.7
META: 0.661

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
167_D170_D6.4331.00
61_E64_K5.7301.00
133_I153_V4.7861.00
108_K305_D4.3521.00
162_Q185_E4.2991.00
26_R150_E4.2871.00
154_K204_E4.2321.00
136_G151_V3.8651.00
32_L73_A3.3251.00
116_Y314_H3.2661.00
126_A187_A3.2531.00
16_A250_I3.1111.00
20_A79_A2.5721.00
193_G201_V2.5271.00
134_A166_F2.4821.00
16_A84_V2.4681.00
71_D232_K2.3791.00
124_E127_R2.3781.00
150_E165_T2.3511.00
224_V235_T2.3161.00
9_R12_D2.3130.99
123_K191_A2.2690.99
238_Q241_G2.2430.99
120_H310_V2.2170.99
16_A245_M2.1570.99
257_I272_V2.1330.99
130_A190_I2.1060.99
183_L187_A2.0780.99
21_G29_W2.0760.99
192_Q197_E2.0730.99
262_P292_Q2.0430.99
34_V238_Q2.0260.99
270_I276_G2.0240.99
182_D185_E2.0160.99
281_L285_Y2.0100.99
38_S69_T2.0070.99
154_K163_S1.9520.98
222_E246_H1.9520.98
18_F245_M1.9410.98
152_Q206_T1.9390.98
117_I122_F1.9260.98
189_L201_V1.9010.98
148_Q210_R1.8980.98
172_S175_D1.8930.98
52_Q61_E1.8750.98
309_S312_Q1.8550.98
51_D61_E1.7990.97
51_D64_K1.7960.97
155_H189_L1.7800.97
20_A76_P1.7290.96
85_R206_T1.7250.96
171_F180_D1.7210.96
181_E185_E1.7130.96
13_P88_L1.7110.96
297_L316_V1.6940.96
261_Y285_Y1.6890.96
25_D28_K1.6630.95
245_M250_I1.6570.95
84_V209_A1.6560.95
127_R184_D1.6520.95
9_R216_E1.6470.95
68_Q248_Q1.6440.95
114_K118_E1.6410.95
227_K231_K1.6240.95
85_R204_E1.6190.95
152_Q208_F1.5790.94
292_Q312_Q1.5610.93
310_V313_Q1.5510.93
50_A61_E1.5440.93
121_G191_A1.5440.93
272_V318_A1.5250.93
152_Q165_T1.5100.92
49_A53_N1.5030.92
131_Y183_L1.5020.92
128_R259_T1.5000.92
55_P58_L1.4940.92
131_Y178_A1.4910.92
228_G231_K1.4870.92
293_D296_T1.4780.91
35_R74_A1.4650.91
164_W185_E1.4640.91
122_F317_M1.4510.91
263_E266_E1.4440.90
95_G113_V1.4290.90
11_L90_V1.4140.89
135_N168_A1.3970.89
280_N315_Y1.3850.88
239_V244_A1.3800.88
43_I46_R1.3760.88
102_N302_V1.3630.88
54_D58_L1.3610.87
127_R179_P1.3570.87
113_V317_M1.3420.87
104_D107_Q1.3390.87
149_I168_A1.3270.86
261_Y269_P1.3100.85
47_L275_Y1.2970.85
127_R131_Y1.2950.85
38_S223_L1.2840.84
272_V315_Y1.2730.84
9_R221_Q1.2560.83
174_R268_G1.2540.83
156_L159_G1.2410.82
13_P251_G1.2300.81
178_A183_L1.2240.81
112_T301_W1.2230.81
188_E192_Q1.2210.81
31_P167_D1.2120.80
72_V216_E1.1990.80
30_T78_D1.1970.80
73_A193_G1.1950.80
216_E239_V1.1950.80
94_V122_F1.1930.79
73_A105_Y1.1910.79
107_Q111_E1.1750.78
88_L205_V1.1640.78
32_L105_Y1.1610.77
11_L88_L1.1530.77
236_L243_A1.1530.77
131_Y176_F1.1470.77
308_P312_Q1.1460.77
308_P313_Q1.1350.76
157_N161_E1.1290.75
230_G244_A1.1260.75
92_G197_E1.1240.75
227_K230_G1.1180.75
171_F234_K1.1100.74
298_L315_Y1.0980.73
188_E191_A1.0880.73
105_Y109_L1.0870.73
115_G119_E1.0810.72
132_N135_N1.0790.72
65_P99_A1.0780.72
18_F84_V1.0780.72
288_P293_D1.0780.72
133_I136_G1.0720.72
76_P79_A1.0650.71
226_D231_K1.0630.71
196_G207_A1.0630.71
45_N67_L1.0620.71
94_V113_V1.0570.70
252_N273_E1.0490.70
110_Q114_K1.0460.70
322_R325_V1.0420.69
126_A256_T1.0420.69
263_E283_K1.0350.69
22_N25_D1.0320.69
108_K112_T1.0280.68
191_A195_S1.0210.68
38_S41_G1.0170.67
92_G196_G1.0150.67
75_L82_L1.0120.67
47_L51_D1.0100.67
54_D57_K1.0100.67
28_K197_E1.0050.67
18_F32_L0.9950.66
3_S101_N0.9880.65
250_I254_L0.9780.64
254_L280_N0.9580.63
136_G207_A0.9520.62
274_P319_V0.9510.62
96_T106_Q0.9450.62
41_G66_N0.9370.61
154_K206_T0.9260.60
49_A52_Q0.9260.60
196_G199_D0.9260.60
152_Q163_S0.9250.60
300_N306_K0.9150.59
115_G118_E0.9150.59
67_L223_L0.9120.59
255_R273_E0.9090.59
71_D75_L0.9090.59
106_Q281_L0.9040.58
50_A54_D0.9020.58
45_N48_K0.9000.58
95_G114_K0.9000.58
49_A59_D0.8930.57
127_R187_A0.8880.57
134_A138_F0.8830.56
302_V317_M0.8830.56
202_L254_L0.8710.55
12_D18_F0.8700.55
205_V271_A0.8670.55
275_Y284_A0.8670.55
216_E248_Q0.8660.55
262_P312_Q0.8600.54
292_Q309_S0.8570.54
289_K302_V0.8530.54
149_I211_L0.8480.53
278_V282_G0.8430.53
24_D28_K0.8410.53
318_A323_G0.8330.52
315_Y319_V0.8300.52
261_Y268_G0.8300.52
228_G315_Y0.8280.52
179_P184_D0.8280.52
59_D63_E0.8180.51
42_T274_P0.8170.51
43_I47_L0.8110.50
60_A67_L0.8110.50
110_Q197_E0.8100.50
133_I205_V0.8100.50
18_F22_N0.8070.50
4_V7_F0.8020.49
67_L225_L0.8020.49
89_R193_G0.8010.49
19_Y83_K0.7960.49
106_Q110_Q0.7940.49
83_K206_T0.7940.49
291_K296_T0.7910.48
132_N137_R0.7910.48
158_D205_V0.7900.48
10_K23_W0.7900.48
302_V307_L0.7890.48
118_E181_E0.7880.48
318_A324_G0.7880.48
317_M321_I0.7880.48
58_L62_I0.7880.48
50_A63_E0.7850.48
176_F207_A0.7830.48
181_E184_D0.7820.48
27_E206_T0.7780.47
226_D261_Y0.7770.47
255_R274_P0.7770.47
133_I254_L0.7740.47
293_D297_L0.7730.47
123_K188_E0.7720.47
207_A297_L0.7720.47
39_V69_T0.7710.47
142_N249_K0.7700.47
179_P254_L0.7690.46
237_Y273_E0.7690.46
111_E118_E0.7640.46
95_G235_T0.7630.46
156_L160_A0.7630.46
43_I64_K0.7610.46
237_Y244_A0.7610.46
82_L217_V0.7590.46
185_E189_L0.7580.46
96_T118_E0.7560.45
139_L273_E0.7560.45
133_I171_F0.7560.45
255_R271_A0.7560.45
57_K62_I0.7540.45
105_Y304_K0.7520.45
214_G295_Y0.7520.45
23_W26_R0.7500.45
90_V321_I0.7480.45
116_Y247_S0.7480.45
88_L251_G0.7450.44
127_R178_A0.7440.44
314_H317_M0.7420.44
151_V207_A0.7420.44
243_A325_V0.7420.44
12_D216_E0.7400.44
288_P291_K0.7390.44
33_K240_N0.7380.44
95_G209_A0.7330.43
156_L204_E0.7320.43
204_E213_K0.7310.43
131_Y179_P0.7270.43
257_I260_W0.7250.43
38_S67_L0.7240.43
17_L21_G0.7210.42
294_F319_V0.7200.42
243_A246_H0.7180.42
261_Y267_L0.7170.42
76_P285_Y0.7150.42
255_R322_R0.7140.42
174_R269_P0.7130.42
114_K177_D0.7130.42
293_D326_F0.7120.42
19_Y32_L0.7060.41
137_R143_R0.7050.41
29_W83_K0.7030.41
19_Y218_F0.7010.41
71_D238_Q0.6980.40
149_I169_L0.6960.40
78_D149_I0.6950.40
144_V229_K0.6950.40
146_A149_I0.6950.40
265_D312_Q0.6940.40
10_K87_T0.6920.40
70_V272_V0.6920.40
136_G172_S0.6910.40
164_W203_L0.6910.40
118_E192_Q0.6870.39
50_A64_K0.6850.39
19_Y28_K0.6840.39
104_D165_T0.6840.39
136_G149_I0.6830.39
304_K307_L0.6820.39
2_A13_P0.6820.39
38_S68_Q0.6810.39
88_L250_I0.6780.39
151_V168_A0.6770.39
258_D286_R0.6760.39
218_F234_K0.6750.38
206_T272_V0.6750.38
148_Q167_D0.6710.38
19_Y208_F0.6710.38
264_A267_L0.6700.38
77_P206_T0.6630.37
6_A98_S0.6620.37
19_Y115_G0.6620.37
277_S318_A0.6620.37
44_S54_D0.6610.37
28_K123_K0.6610.37
40_R279_T0.6580.37
136_G170_D0.6550.37
130_A153_V0.6530.37
47_L52_Q0.6520.37
244_A280_N0.6460.36
212_G215_Q0.6460.36
70_V248_Q0.6450.36
127_R189_L0.6450.36
83_K208_F0.6440.36
295_Y301_W0.6430.36
39_V248_Q0.6420.36
56_A59_D0.6420.36
146_A209_A0.6400.36
41_G70_V0.6360.35
55_P61_E0.6340.35
41_G45_N0.6320.35
20_A93_G0.6300.35
241_G263_E0.6290.35
136_G197_E0.6280.35
261_Y281_L0.6280.35
118_E161_E0.6250.34
38_S70_V0.6250.34
22_N28_K0.6230.34
89_R211_L0.6230.34
259_T264_A0.6220.34
148_Q153_V0.6220.34
51_D58_L0.6200.34
203_L240_N0.6200.34
113_V193_G0.6190.34
131_Y171_F0.6190.34
256_T269_P0.6180.34
138_F166_F0.6180.34
51_D59_D0.6170.34
218_F281_L0.6170.34
40_R65_P0.6150.34
252_N256_T0.6140.34
27_E192_Q0.6130.33
9_R218_F0.6120.33
112_T305_D0.6120.33
39_V68_Q0.6110.33
11_L251_G0.6100.33
279_T284_A0.6070.33
16_A20_A0.6060.33
315_Y323_G0.6050.33
112_T317_M0.6050.33
144_V200_F0.6040.33
212_G236_L0.6040.33
6_A70_V0.6030.33
107_Q263_E0.6020.33
185_E188_E0.6010.32
315_Y324_G0.6010.32
307_L313_Q0.6000.32
20_A32_L0.5980.32
184_D192_Q0.5960.32
12_D95_G0.5960.32
294_F299_D0.5940.32
124_E218_F0.5930.32
37_K43_I0.5920.32
156_L164_W0.5910.32
76_P109_L0.5910.32
306_K313_Q0.5880.32
127_R240_N0.5870.31
298_L319_V0.5860.31
25_D33_K0.5860.31
151_V157_N0.5850.31
305_D308_P0.5840.31
187_A195_S0.5830.31
47_L61_E0.5830.31
58_L61_E0.5820.31
14_S87_T0.5820.31
322_R327_G0.5810.31
2_A115_G0.5810.31
25_D205_V0.5810.31
274_P322_R0.5810.31
41_G248_Q0.5800.31
95_G224_V0.5800.31
37_K70_V0.5790.31
107_Q162_Q0.5790.31
28_K310_V0.5780.31
86_F138_F0.5780.31
304_K308_P0.5750.31
300_N312_Q0.5740.30
276_G286_R0.5710.30
36_E69_T0.5710.30
148_Q296_T0.5710.30
178_A188_E0.5710.30
37_K46_R0.5690.30
171_F183_L0.5660.30
94_V317_M0.5660.30
4_V327_G0.5650.30
34_V243_A0.5650.30
100_C279_T0.5640.30
178_A182_D0.5640.30
25_D113_V0.5630.30
27_E175_D0.5630.30
311_E314_H0.5620.30
164_W189_L0.5600.29
53_N59_D0.5590.29
30_T123_K0.5580.29
273_E322_R0.5570.29
157_N244_A0.5560.29
155_H164_W0.5560.29
30_T188_E0.5560.29
136_G226_D0.5560.29
302_V313_Q0.5560.29
156_L189_L0.5550.29
97_P271_A0.5530.29
177_D254_L0.5530.29
6_A247_S0.5520.29
53_N61_E0.5510.29
110_Q181_E0.5500.29
84_V139_L0.5500.29
320_L324_G0.5500.29
149_I227_K0.5500.29
319_V326_F0.5480.29
205_V290_D0.5470.28
221_Q227_K0.5460.28
11_L194_L0.5450.28
123_K163_S0.5450.28
182_D238_Q0.5430.28
270_I285_Y0.5430.28
12_D35_R0.5410.28
106_Q267_L0.5410.28
105_Y305_D0.5390.28
71_D138_F0.5380.28
171_F178_A0.5360.28
133_I256_T0.5360.28
10_K21_G0.5360.28
49_A206_T0.5360.28
284_A293_D0.5340.28
178_A238_Q0.5330.27
28_K114_K0.5330.27
135_N143_R0.5330.27
39_V43_I0.5320.27
223_L288_P0.5320.27
23_W208_F0.5300.27
10_K15_D0.5280.27
25_D192_Q0.5270.27
24_D150_E0.5270.27
156_L161_E0.5260.27
39_V70_V0.5260.27
221_Q301_W0.5250.27
295_Y315_Y0.5250.27
49_A102_N0.5250.27
97_P101_N0.5250.27
97_P100_C0.5230.27
13_P38_S0.5220.27
111_E188_E0.5220.27
200_F204_E0.5220.27
21_G83_K0.5200.27
123_K184_D0.5200.27
114_K170_D0.5200.27
70_V237_Y0.5190.27
13_P93_G0.5190.27
63_E223_L0.5150.26
261_Y264_A0.5150.26
231_K280_N0.5140.26
39_V216_E0.5130.26
18_F216_E0.5130.26
262_P315_Y0.5120.26
5_L10_K0.5120.26
218_F221_Q0.5110.26
195_S265_D0.5110.26
180_D183_L0.5100.26
1_T308_P0.5090.26
2_A327_G0.5080.26
243_A248_Q0.5080.26
50_A62_I0.5070.26
15_D237_Y0.5070.26
52_Q283_K0.5060.26
144_V272_V0.5060.26
69_T248_Q0.5060.26
100_C251_G0.5050.26
23_W138_F0.5050.26
102_N254_L0.5040.26
52_Q183_L0.5040.26
286_R323_G0.5030.25
119_E310_V0.5020.25
318_A322_R0.5010.25
61_E280_N0.5000.25
137_R256_T0.5000.25
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3ctdA 3 0.107 16.3 0.975 Contact Map
2hiqA 2 0.2813 8.7 0.978 Contact Map
1q14A 3 0.2905 5.8 0.979 Contact Map
3wxoA 2 0.2446 4.2 0.981 Contact Map
3ogiA 1 0.1743 3.6 0.981 Contact Map
5a3aA 1 0.2997 3.5 0.981 Contact Map
4rmhA 1 0.2813 3.2 0.982 Contact Map
2khxA 1 0.1101 3.1 0.982 Contact Map
4ollA 1 0.2232 3.1 0.982 Contact Map
1m2kA 1 0.2752 3.1 0.982 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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