GREMLIN Database
DUF2459 - Protein of unknown function (DUF2459)
PFAM: PF09601 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 171 (170)
Sequences: 4123 (2999)
Seq/√Len: 230.0
META: 0.872

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
109_F156_A6.8781.00
49_R138_E3.4631.00
99_S102_Q3.0301.00
147_N155_R2.9651.00
12_I156_A2.2471.00
50_G54_E2.2001.00
49_R53_L2.1761.00
49_R75_P2.1581.00
113_S139_A2.1321.00
165_S168_T2.1251.00
4_V13_V2.0801.00
55_T65_T2.0201.00
103_Y107_V2.0081.00
48_D74_G2.0021.00
17_K24_R1.9911.00
10_T153_T1.9851.00
50_G68_K1.9591.00
106_L156_A1.9561.00
67_L71_F1.8731.00
117_D121_R1.8471.00
77_V125_I1.8051.00
154_A164_T1.7821.00
79_H134_D1.7411.00
62_T65_T1.6521.00
79_H130_Y1.6311.00
44_F78_M1.6151.00
144_S147_N1.5651.00
124_R135_A1.5581.00
32_F35_P1.5441.00
61_L66_A1.5291.00
156_A159_A1.5241.00
128_G134_D1.4841.00
73_P144_S1.4491.00
17_K22_D1.4481.00
45_G52_Y1.4481.00
21_L111_R1.4351.00
63_P67_L1.4341.00
130_Y134_D1.4261.00
115_A140_R1.4241.00
113_S141_G1.4241.00
27_F114_F1.4141.00
111_R116_R1.4061.00
76_S141_G1.3921.00
154_A158_R1.3921.00
14_L110_I1.3891.00
100_P104_R1.3891.00
1_I98_L1.3881.00
11_D43_A1.3861.00
148_T151_T1.3651.00
109_F159_A1.3621.00
46_W76_S1.3581.00
78_M114_F1.3291.00
14_L44_F1.3071.00
154_A168_T1.2811.00
143_Y148_T1.2771.00
53_L125_I1.2641.00
115_A123_I1.2611.00
51_F61_L1.2501.00
57_T60_D1.2391.00
98_L162_L1.2351.00
78_M113_S1.2011.00
109_F155_R1.1821.00
126_G129_G1.1721.00
108_A112_A1.1701.00
8_V58_W1.1661.00
42_L82_G1.1621.00
147_N151_T1.1581.00
26_L122_P1.1561.00
78_M139_A1.1561.00
74_G144_S1.1551.00
4_V93_V1.1521.00
21_L26_L1.1281.00
30_S33_A1.1141.00
68_K74_G1.1081.00
116_R122_P1.1051.00
141_G147_N1.1011.00
11_D45_G1.0991.00
54_E65_T1.0941.00
10_T149_C1.0871.00
106_L160_A1.0821.00
79_H136_F1.0771.00
46_W141_G1.0701.00
55_T62_T1.0571.00
94_R97_R1.0511.00
129_G134_D1.0491.00
31_D137_Y1.0301.00
123_I138_E1.0191.00
18_A21_L1.0191.00
43_A83_Y1.0161.00
151_T168_T1.0141.00
152_W156_A1.0121.00
64_S68_K1.0111.00
11_D52_Y1.0111.00
2_Y95_E0.9951.00
44_F114_F0.9831.00
39_A82_G0.9791.00
157_L162_L0.9781.00
22_D25_A0.9721.00
151_T154_A0.9711.00
44_F80_V0.9701.00
16_V21_L0.9641.00
44_F110_I0.9581.00
115_A138_E0.9461.00
109_F113_S0.9431.00
89_P95_E0.9371.00
154_A165_S0.9291.00
63_P66_A0.9171.00
105_R159_A0.9141.00
105_R161_G0.9111.00
101_E105_R0.9070.99
117_D123_I0.9020.99
104_R108_A0.8950.99
15_P18_A0.8950.99
6_N11_D0.8930.99
13_V43_A0.8920.99
19_E23_W0.8780.99
114_F137_Y0.8770.99
118_A121_R0.8690.99
129_G132_P0.8680.99
85_P88_A0.8680.99
20_L104_R0.8580.99
16_V24_R0.8540.99
3_V10_T0.8500.99
114_F122_P0.8490.99
52_Y141_G0.8450.99
117_D120_G0.8380.99
14_L42_L0.8330.99
113_S156_A0.8300.99
120_G123_I0.8280.99
106_L110_I0.8260.99
98_L103_Y0.8220.99
10_T143_Y0.8210.99
28_P37_P0.8150.99
98_L102_Q0.8120.99
26_L116_R0.8070.99
52_Y136_F0.7990.99
20_L107_V0.7920.99
26_L111_R0.7820.99
29_P36_D0.7790.98
50_G65_T0.7780.98
6_N79_H0.7770.98
5_S92_D0.7730.98
66_A69_A0.7720.98
22_D26_L0.7700.98
59_A62_T0.7570.98
76_S113_S0.7560.98
55_T61_L0.7460.98
48_D69_A0.7420.98
72_G142_R0.7360.98
54_E62_T0.7360.98
80_V114_F0.7340.98
6_N91_S0.7280.98
4_V43_A0.7280.98
55_T60_D0.7210.97
17_K21_L0.7180.97
50_G74_G0.7180.97
55_T58_W0.7150.97
101_E104_R0.7150.97
29_P32_F0.7140.97
46_W52_Y0.7060.97
20_L24_R0.7050.97
34_A39_A0.7040.97
111_R122_P0.7030.97
16_V40_R0.6930.97
4_V11_D0.6900.97
151_T155_R0.6820.96
52_Y77_V0.6800.96
5_S51_F0.6790.96
84_P88_A0.6780.96
29_P34_A0.6770.96
87_P108_A0.6750.96
48_D75_P0.6740.96
130_Y136_F0.6730.96
89_P93_V0.6570.96
21_L25_A0.6560.96
32_F36_D0.6550.96
155_R159_A0.6430.95
16_V19_E0.6400.95
102_Q105_R0.6390.95
84_P87_P0.6330.95
86_P95_E0.6310.95
107_V111_R0.6300.95
18_A103_Y0.6280.94
98_L106_L0.6280.94
71_F74_G0.6260.94
119_D140_R0.6260.94
21_L24_R0.6240.94
36_D84_P0.6240.94
23_W26_L0.6210.94
28_P82_G0.6210.94
127_G130_Y0.6200.94
46_W152_W0.6190.94
61_L65_T0.6180.94
65_T68_K0.6180.94
46_W78_M0.6150.94
51_F62_T0.6140.94
46_W143_Y0.6120.94
51_F55_T0.6090.94
73_P142_R0.6080.93
102_Q161_G0.6060.93
81_T133_N0.6050.93
101_E112_A0.6050.93
11_D81_T0.6040.93
143_Y149_C0.6000.93
54_E59_A0.5910.92
49_R77_V0.5910.92
3_V165_S0.5900.92
56_P61_L0.5900.92
49_R54_E0.5900.92
43_A81_T0.5850.92
19_E100_P0.5830.92
24_R28_P0.5790.92
78_M141_G0.5770.92
98_L160_A0.5760.92
119_D123_I0.5720.91
18_A24_R0.5690.91
153_T156_A0.5690.91
67_L73_P0.5650.91
72_G144_S0.5580.90
105_R108_A0.5560.90
68_K72_G0.5530.90
18_A23_W0.5510.90
34_A37_P0.5500.90
77_V81_T0.5490.89
18_A22_D0.5490.89
25_A28_P0.5490.89
125_I128_G0.5480.89
66_A70_L0.5470.89
70_L145_L0.5460.89
17_K20_L0.5450.89
102_Q163_P0.5440.89
81_T134_D0.5420.89
151_T156_A0.5400.89
75_P142_R0.5390.89
4_V10_T0.5380.89
53_L130_Y0.5340.88
102_Q162_L0.5330.88
16_V29_P0.5280.88
53_L127_G0.5240.87
163_P169_P0.5230.87
26_L121_R0.5200.87
33_A36_D0.5160.86
12_I110_I0.5110.86
158_R165_S0.5100.86
23_W28_P0.5100.86
165_S170_F0.5100.86
24_R29_P0.5080.86
102_Q106_L0.5070.85
79_H129_G0.5050.85
162_L165_S0.5040.85
8_V55_T0.5030.85
151_T169_P0.5020.85
47_G152_W0.5010.85
101_E118_A0.5010.85
100_P112_A0.5000.85
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4onzA 1 0.3567 12.5 0.946 Contact Map
2wp7A 2 0.5789 11.6 0.947 Contact Map
3vn5A 1 0.4035 10.6 0.948 Contact Map
4ly1A 2 0.8655 8.6 0.95 Contact Map
4okeA 2 0.3743 7.6 0.951 Contact Map
4bkxB 1 0.8363 5.3 0.955 Contact Map
2e1vA 1 0.5146 4.1 0.957 Contact Map
3rssA 4 0.538 3.9 0.958 Contact Map
3kstA 1 0.5789 3.7 0.958 Contact Map
3ligA 2 0.4561 3.6 0.958 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0068 seconds.