GREMLIN Database
Spore_III_AF - Stage III sporulation protein AF (Spore_III_AF)
PFAM: PF09581 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 189 (184)
Sequences: 1521 (1374)
Seq/√Len: 101.3
META: 0.63

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
123_K187_E4.1031.00
3_I34_A3.8801.00
96_L171_I3.3311.00
143_E146_E3.1861.00
3_I31_L2.9811.00
107_E125_E2.8851.00
82_E86_E2.8381.00
125_E187_E2.7311.00
68_E71_E2.5921.00
89_E108_V2.4081.00
103_V106_V2.2631.00
173_E183_E2.2461.00
69_G72_L2.1711.00
76_Q79_Q2.1601.00
81_L85_E2.1311.00
86_E89_E2.1221.00
91_Q179_Y2.1071.00
92_I175_L2.0961.00
172_K176_S2.0781.00
109_E123_K2.0101.00
75_E78_E1.9461.00
91_Q95_L1.9371.00
72_L76_Q1.8241.00
16_N20_K1.8231.00
82_E85_E1.8221.00
14_L23_V1.8151.00
74_E77_Q1.6941.00
84_Y119_G1.6921.00
12_M15_P1.6671.00
20_K24_K1.6511.00
72_L79_Q1.6481.00
167_E170_E1.6101.00
66_E70_E1.5951.00
145_V148_V1.5881.00
17_G20_K1.5541.00
90_K93_K1.5361.00
102_E129_K1.5291.00
105_D127_T1.5091.00
142_V145_V1.5031.00
97_E103_V1.4961.00
166_E170_E1.4691.00
92_I124_V1.4631.00
32_I40_L1.4581.00
182_S185_K1.4361.00
3_I11_E1.4081.00
99_E170_E1.3991.00
28_G32_I1.3891.00
172_K183_E1.3640.99
25_L30_L1.3630.99
92_I96_L1.3560.99
93_K97_E1.3550.99
128_L171_I1.3460.99
94_E97_E1.3440.99
111_E122_K1.3430.99
70_E73_K1.3420.99
31_L96_L1.3340.99
181_I185_K1.3290.99
156_E159_E1.3210.99
65_I69_G1.3080.99
10_L27_L1.2950.99
101_Y167_E1.2850.99
9_F12_M1.2750.99
74_E78_E1.2630.99
83_Q92_I1.2550.99
96_L101_Y1.2510.99
95_L174_L1.2500.99
71_E75_E1.2330.99
66_E69_G1.2290.99
31_L34_A1.2160.99
145_V150_I1.1930.99
63_E66_E1.1880.98
126_V175_L1.1650.98
169_E173_E1.1650.98
109_E125_E1.1500.98
3_I10_L1.1470.98
95_L178_F1.1350.98
83_Q87_Q1.1320.98
109_E122_K1.1310.98
10_L22_Y1.1150.98
153_T157_E1.1150.98
7_A12_M1.0960.97
64_E68_E1.0940.97
93_K124_V1.0760.97
73_K76_Q1.0750.97
39_I136_E1.0740.97
7_A11_E1.0630.97
169_E183_E1.0450.97
79_Q96_L1.0430.97
60_P63_E1.0430.97
72_L75_E1.0420.97
69_G79_Q1.0360.96
168_E172_K1.0360.96
30_L34_A1.0360.96
87_Q90_K1.0230.96
175_L186_I1.0230.96
47_F88_L1.0070.96
12_M26_V1.0030.96
119_G179_Y0.9980.96
117_D120_E0.9940.95
153_T156_E0.9870.95
46_D179_Y0.9830.95
180_G185_K0.9690.95
84_Y179_Y0.9660.95
47_F50_E0.9480.94
4_V16_N0.9390.94
69_G73_K0.9310.94
80_I83_Q0.9280.93
4_V31_L0.9250.93
108_V112_E0.9200.93
169_E172_K0.9200.93
88_L171_I0.9180.93
176_S182_S0.9170.93
7_A31_L0.9090.93
11_E15_P0.9020.92
137_E144_P0.8970.92
85_E179_Y0.8870.92
90_K94_E0.8770.91
13_L83_Q0.8710.91
30_L99_E0.8690.91
39_I43_L0.8670.91
56_E59_E0.8620.91
85_E112_E0.8610.91
110_I119_G0.8530.90
78_E82_E0.8430.90
35_I77_Q0.8430.90
10_L21_K0.8330.89
25_L84_Y0.8260.89
126_V179_Y0.8220.89
12_M19_M0.8200.88
73_K77_Q0.8180.88
179_Y188_V0.8180.88
24_K180_G0.8120.88
5_L21_K0.8100.88
59_E69_G0.8080.88
136_E139_E0.7990.87
4_V12_M0.7860.86
78_E81_L0.7820.86
97_E145_V0.7810.86
7_A27_L0.7800.86
10_L101_Y0.7800.86
103_V126_V0.7790.86
37_S41_K0.7760.85
99_E106_V0.7740.85
9_F21_K0.7720.85
162_E177_E0.7700.85
161_E181_I0.7630.85
33_L37_S0.7610.84
96_L106_V0.7550.84
111_E120_E0.7490.83
104_A129_K0.7390.83
37_S40_L0.7380.83
63_E160_E0.7310.82
32_I51_L0.7290.82
57_S60_P0.7290.82
3_I12_M0.7290.82
67_E72_L0.7230.81
180_G187_E0.7200.81
27_L34_A0.7160.81
155_E162_E0.7160.81
136_E146_E0.7140.80
22_Y97_E0.7110.80
11_E14_L0.7040.79
11_E34_A0.7010.79
2_L177_E0.6980.79
6_L176_S0.6980.79
154_G157_E0.6960.79
2_L29_L0.6960.79
30_L180_G0.6950.79
32_I84_Y0.6930.78
80_I112_E0.6910.78
126_V171_I0.6900.78
174_L178_F0.6850.78
87_Q96_L0.6780.77
12_M33_L0.6780.77
178_F181_I0.6730.76
139_E142_V0.6710.76
84_Y118_F0.6710.76
139_E143_E0.6700.76
136_E153_T0.6690.76
134_E139_E0.6610.75
113_D116_S0.6590.75
27_L108_V0.6550.74
12_M20_K0.6530.74
6_L81_L0.6480.74
11_E19_M0.6470.73
111_E116_S0.6460.73
44_N59_E0.6460.73
88_L109_E0.6460.73
111_E117_D0.6460.73
35_I47_F0.6420.73
88_L108_V0.6410.73
95_L179_Y0.6410.73
168_E188_V0.6310.72
9_F30_L0.6310.72
12_M147_P0.6280.71
4_V87_Q0.6250.71
81_L110_I0.6240.71
56_E71_E0.6230.71
184_E188_V0.6210.70
137_E157_E0.6160.70
49_L82_E0.6140.70
96_L103_V0.6130.69
91_Q178_F0.6090.69
11_E24_K0.6080.69
66_E92_I0.6070.69
9_F181_I0.6050.68
68_E72_L0.6030.68
22_Y103_V0.6020.68
11_E20_K0.6000.68
7_A84_Y0.5940.67
161_E183_E0.5940.67
134_E155_E0.5920.67
92_I106_V0.5910.67
7_A16_N0.5890.66
5_L113_D0.5880.66
3_I30_L0.5860.66
64_E74_E0.5860.66
111_E114_E0.5840.66
22_Y25_L0.5830.66
9_F95_L0.5800.65
21_K33_L0.5790.65
2_L6_L0.5740.64
158_E161_E0.5740.64
104_A126_V0.5700.64
168_E187_E0.5690.64
4_V11_E0.5660.63
184_E187_E0.5650.63
7_A24_K0.5650.63
110_I113_D0.5600.63
124_V181_I0.5580.62
57_S162_E0.5570.62
7_A25_L0.5560.62
1_I14_L0.5560.62
1_I179_Y0.5550.62
40_L46_D0.5530.62
89_E93_K0.5510.61
96_L126_V0.5500.61
18_S102_E0.5500.61
14_L30_L0.5500.61
171_I188_V0.5500.61
9_F175_L0.5490.61
31_L35_I0.5470.61
12_M27_L0.5470.61
3_I9_F0.5470.61
13_L29_L0.5450.60
143_E157_E0.5380.59
85_E92_I0.5380.59
130_E155_E0.5370.59
5_L179_Y0.5370.59
138_E181_I0.5350.59
121_I186_I0.5340.59
167_E171_I0.5310.58
52_A110_I0.5310.58
1_I47_F0.5310.58
24_K29_L0.5310.58
49_L166_E0.5310.58
112_E163_E0.5270.58
25_L76_Q0.5270.58
109_E112_E0.5260.58
17_G87_Q0.5260.58
32_I104_A0.5230.57
11_E26_V0.5230.57
95_L175_L0.5220.57
101_Y130_E0.5220.57
9_F29_L0.5200.57
160_E170_E0.5160.56
10_L180_G0.5160.56
68_E90_K0.5150.56
83_Q86_E0.5110.56
155_E158_E0.5110.56
2_L93_K0.5110.56
67_E74_E0.5100.55
13_L40_L0.5090.55
71_E74_E0.5070.55
27_L85_E0.5050.55
155_E172_K0.5000.54
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4bpeC 1 0.4603 18.5 0.918 Contact Map
1n91A 1 0.3862 18.2 0.918 Contact Map
4w23D 1 0.4603 14.2 0.922 Contact Map
4bpeP 1 0.291 10 0.927 Contact Map
3mb2B 3 0.2275 9.8 0.927 Contact Map
3zeyX 1 0.4444 9.2 0.928 Contact Map
4c00A 1 0.619 9.2 0.928 Contact Map
3r9jC 2 0.2751 8.8 0.929 Contact Map
2kxoA 2 0.3439 8.2 0.93 Contact Map
3j7aD 1 0.2593 8.2 0.93 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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