GREMLIN Database
DUF2385 - Protein of unknown function (DUF2385)
PFAM: PF09539 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 96 (96)
Sequences: 1884 (1029)
Seq/√Len: 105.0
META: 0.897

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
17_H56_F4.1211.00
36_Q40_D3.3061.00
42_E48_R2.8471.00
30_K34_E2.1641.00
20_R27_E2.1541.00
63_F81_Y2.1511.00
67_Y74_A2.0801.00
21_E28_G2.0751.00
49_R53_V2.0671.00
31_W85_G2.0171.00
8_R84_E2.0031.00
35_M39_L1.9911.00
20_R78_I1.9101.00
87_K91_D1.9041.00
18_Y22_L1.8441.00
3_D48_R1.8361.00
29_Q33_D1.7671.00
12_I85_G1.6931.00
31_W51_R1.6881.00
3_D7_L1.6121.00
55_A64_Q1.5631.00
2_Y5_D1.5331.00
63_F67_Y1.4791.00
8_R11_E1.3861.00
73_A76_E1.3611.00
78_I82_L1.3591.00
86_A89_A1.3410.99
79_R83_E1.3250.99
8_R12_I1.2380.99
48_R52_L1.1870.99
11_E15_A1.1820.99
89_A93_A1.1560.98
29_Q32_R1.1530.98
17_H60_Y1.1490.98
33_D36_Q1.1290.98
32_R56_F1.1230.98
67_Y71_T1.1170.98
81_Y84_E1.1030.98
45_S52_L1.1030.98
30_K33_D1.0930.98
21_E26_N1.0930.98
75_R79_R1.0900.98
86_A90_R1.0770.97
83_E87_K1.0600.97
51_R55_A1.0590.97
37_A41_A1.0080.96
15_A81_Y1.0030.96
7_L55_A0.9920.96
10_A35_M0.9910.96
35_M85_G0.9810.96
85_G88_L0.9770.95
37_A40_D0.9610.95
21_E64_Q0.9580.95
83_E86_A0.9480.95
25_A29_Q0.9290.94
19_L24_G0.9240.94
39_L61_R0.9210.94
34_E37_A0.9190.94
44_P56_F0.9180.94
5_D91_D0.8880.93
12_I16_L0.8860.93
46_G50_A0.8780.92
20_R28_G0.8720.92
87_K94_A0.8710.92
16_L21_E0.8700.92
15_A63_F0.8600.91
63_F77_A0.8400.90
26_N31_W0.8380.90
13_L17_H0.8350.90
10_A68_R0.8220.89
76_E80_R0.8110.89
40_D49_R0.8090.89
61_R66_T0.8000.88
61_R64_Q0.7880.87
6_L10_A0.7840.87
44_P53_V0.7830.87
39_L49_R0.7750.86
62_G66_T0.7740.86
28_G89_A0.7700.86
19_L26_N0.7700.86
44_P49_R0.7660.86
21_E29_Q0.7570.85
21_E60_Y0.7550.85
24_G78_I0.7530.85
22_L68_R0.7520.85
62_G68_R0.7430.84
11_E58_R0.7410.84
88_L96_Y0.7410.84
20_R79_R0.7300.83
15_A21_E0.7100.81
49_R57_N0.6850.79
44_P60_Y0.6810.78
58_R62_G0.6810.78
8_R63_F0.6750.78
18_Y59_G0.6710.77
25_A75_R0.6690.77
54_A58_R0.6680.77
41_A52_L0.6630.77
21_E43_A0.6610.76
67_Y84_E0.6480.75
28_G82_L0.6470.75
36_Q60_Y0.6410.74
27_E72_P0.6350.74
4_P40_D0.6340.73
75_R82_L0.6340.73
16_L78_I0.6270.73
65_R85_G0.6250.72
37_A72_P0.6180.71
63_F84_E0.6180.71
25_A30_K0.6180.71
6_L59_G0.6180.71
67_Y73_A0.6140.71
76_E79_R0.6080.70
15_A67_Y0.6060.70
82_L86_A0.6030.70
77_A81_Y0.5970.69
5_D50_A0.5960.69
69_T90_R0.5950.69
46_G49_R0.5940.68
51_R54_A0.5900.68
24_G61_R0.5880.68
18_Y31_W0.5830.67
27_E47_E0.5740.66
19_L74_A0.5710.65
74_A81_Y0.5690.65
47_E56_F0.5680.65
72_P75_R0.5660.65
16_L89_A0.5600.64
20_R24_G0.5600.64
15_A59_G0.5600.64
50_A54_A0.5520.63
85_G89_A0.5520.63
45_S50_A0.5480.62
53_V81_Y0.5430.62
12_I18_Y0.5430.62
47_E55_A0.5340.60
25_A82_L0.5330.60
7_L10_A0.5320.60
42_E85_G0.5230.59
13_L29_Q0.5130.57
17_H21_E0.5110.57
17_H65_R0.5050.56
8_R15_A0.5020.56
17_H20_R0.5000.55
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2nr5A 4 0.4583 32.9 0.903 Contact Map
2p6rA 1 0.875 26.5 0.907 Contact Map
2omoA 2 0.7188 12.2 0.92 Contact Map
2kdnA 1 0.8021 9.9 0.923 Contact Map
4xaiA 2 0.5104 7.9 0.927 Contact Map
2asyA 2 0.6146 6.4 0.93 Contact Map
2l6nA 1 0.5833 6.3 0.93 Contact Map
1pixA 4 0.7917 6.1 0.931 Contact Map
1or4A 2 0.6667 5.2 0.933 Contact Map
3utkA 1 0.75 5.2 0.933 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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