GREMLIN Database
DUF2461 - Conserved hypothetical protein (DUF2461)
PFAM: PF09365 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 208 (207)
Sequences: 17989 (12642)
Seq/√Len: 878.7
META: 0.908

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
156_A167_I3.4041.00
8_E24_R3.3941.00
21_N24_R3.3581.00
152_K172_R2.9091.00
203_A207_D2.7471.00
26_E29_R2.5401.00
42_E196_A2.5211.00
29_R33_L2.4341.00
128_N131_E2.4341.00
15_R19_E2.3761.00
129_P166_A2.3281.00
36_V81_A2.1991.00
133_R166_A2.1961.00
23_A27_E2.1881.00
19_E22_K2.1281.00
108_G202_L2.1281.00
115_P119_K2.0641.00
100_E103_G1.9911.00
179_R198_A1.9671.00
119_K123_E1.9611.00
182_P185_E1.9401.00
155_R162_K1.8781.00
42_E193_D1.8231.00
4_D8_E1.8171.00
19_E26_E1.8121.00
194_E197_E1.7861.00
32_M81_A1.7401.00
82_S94_G1.7241.00
155_R158_R1.6881.00
126_D164_H1.6611.00
185_E194_E1.6571.00
124_A128_N1.6251.00
194_E198_A1.6111.00
133_R137_A1.6051.00
38_E192_L1.5971.00
54_P57_G1.5901.00
114_E117_Q1.5651.00
110_I121_I1.4971.00
177_A205_L1.4921.00
177_A202_L1.4701.00
8_E17_W1.4561.00
32_M79_F1.4201.00
141_F177_A1.4191.00
42_E45_K1.4181.00
192_L196_A1.4171.00
112_M174_S1.4081.00
37_E41_P1.3731.00
22_K26_E1.3671.00
144_Y201_A1.3601.00
123_E127_D1.3481.00
139_K143_K1.3431.00
23_A26_E1.3381.00
33_L59_L1.3301.00
80_G98_H1.3231.00
10_K77_T1.3111.00
26_E30_A1.3051.00
186_V191_F1.2971.00
106_V195_V1.2891.00
98_H105_F1.2891.00
124_A127_D1.2871.00
119_K160_F1.2671.00
25_Y29_R1.2461.00
16_E19_E1.2441.00
66_V73_T1.2361.00
195_V199_F1.2291.00
30_A34_A1.2231.00
49_E85_R1.2211.00
43_L46_F1.2131.00
144_Y198_A1.2111.00
30_A33_L1.1931.00
156_A162_K1.1891.00
129_P132_L1.1891.00
28_V31_P1.1861.00
37_E55_A1.1821.00
46_F200_R1.1821.00
103_G180_E1.1811.00
12_N17_W1.1801.00
198_A201_A1.1771.00
78_H98_H1.1741.00
19_E63_Y1.1721.00
73_T77_T1.1701.00
66_V72_K1.1591.00
105_F178_G1.1581.00
136_L169_L1.1551.00
69_S72_K1.1521.00
161_D164_H1.1251.00
10_K102_G1.1241.00
22_K63_Y1.1221.00
190_D194_E1.1211.00
10_K100_E1.1211.00
102_G183_D1.1191.00
121_I125_I1.1091.00
197_E201_A1.0981.00
196_A200_R1.0791.00
91_H114_E1.0771.00
132_L169_L1.0771.00
107_G178_G1.0621.00
28_V79_F1.0581.00
144_Y179_R1.0551.00
141_F144_Y1.0531.00
27_E30_A1.0531.00
150_G174_S1.0531.00
156_A171_K1.0461.00
123_E160_F1.0401.00
2_T31_P1.0341.00
181_L198_A1.0271.00
168_E171_K1.0261.00
24_R27_E1.0241.00
151_E173_K1.0231.00
63_Y66_V1.0221.00
84_H87_G1.0171.00
2_T28_V0.9971.00
190_D193_D0.9911.00
106_V198_A0.9881.00
40_I44_G0.9751.00
118_L175_F0.9581.00
192_L195_V0.9571.00
129_P133_R0.9561.00
135_I205_L0.9511.00
14_N19_E0.9511.00
141_F145_F0.9481.00
110_I113_P0.9431.00
42_E192_L0.9421.00
104_S186_V0.9421.00
130_E134_K0.9401.00
77_T100_E0.9401.00
33_L37_E0.9381.00
37_E54_P0.9351.00
69_S73_T0.9351.00
39_L196_A0.9291.00
152_K168_E0.9261.00
46_F196_A0.9221.00
10_K101_P0.9211.00
60_F78_H0.9211.00
35_L195_V0.9181.00
125_I132_L0.9161.00
98_H103_G0.9161.00
191_F195_V0.9161.00
104_S183_D0.9141.00
131_E135_I0.9111.00
39_L195_V0.9101.00
149_E176_I0.9031.00
38_E42_E0.9021.00
63_Y75_Y0.9011.00
113_P121_I0.9011.00
76_K80_G0.8971.00
179_R194_E0.8921.00
93_P206_V0.8891.00
111_Y176_I0.8891.00
60_F80_G0.8841.00
85_R92_G0.8831.00
152_K171_K0.8791.00
18_F62_I0.8741.00
166_A169_L0.8741.00
53_L57_G0.8701.00
64_R73_T0.8671.00
66_V69_S0.8641.00
169_L172_R0.8621.00
108_G206_V0.8611.00
185_E188_S0.8601.00
34_A38_E0.8511.00
131_E134_K0.8501.00
184_A187_L0.8421.00
76_K98_H0.8411.00
150_G172_R0.8331.00
37_E40_I0.8321.00
7_R11_A0.8321.00
133_R169_L0.8301.00
106_V181_L0.8291.00
130_E133_R0.8291.00
7_R183_D0.8271.00
142_K145_F0.8261.00
97_F191_F0.8251.00
29_R63_Y0.8181.00
60_F96_Y0.8181.00
123_E126_D0.8171.00
167_I171_K0.8051.00
40_I58_S0.8041.00
153_L173_K0.7931.00
133_R168_E0.7931.00
26_E33_L0.7841.00
5_F18_F0.7821.00
152_K173_K0.7791.00
115_P173_K0.7681.00
95_Y203_A0.7661.00
41_P44_G0.7661.00
137_A142_K0.7561.00
36_V59_L0.7521.00
172_R175_F0.7511.00
140_A201_A0.7511.00
117_Q120_R0.7501.00
140_A144_Y0.7481.00
6_L28_V0.7481.00
38_E41_P0.7421.00
39_L192_L0.7401.00
149_E178_G0.7381.00
25_Y62_I0.7291.00
95_Y199_F0.7241.00
32_M59_L0.7211.00
149_E152_K0.7171.00
100_E183_D0.7161.00
139_K142_K0.7131.00
8_E11_A0.7131.00
138_A141_F0.7131.00
163_D167_I0.7041.00
138_A142_K0.7011.00
88_K91_H0.6931.00
132_L136_L0.6921.00
183_D186_V0.6911.00
140_A143_K0.6911.00
181_L194_E0.6901.00
148_L152_K0.6901.00
107_G176_I0.6871.00
116_D119_K0.6761.00
3_L104_S0.6751.00
148_L176_I0.6731.00
81_A97_F0.6731.00
7_R101_P0.6701.00
44_G51_V0.6661.00
197_E200_R0.6661.00
155_R171_K0.6631.00
8_E12_N0.6631.00
121_I175_F0.6601.00
3_L183_D0.6601.00
148_L172_R0.6581.00
18_F63_Y0.6541.00
9_L62_I0.6521.00
14_N20_A0.6491.00
31_P34_A0.6481.00
138_A143_K0.6461.00
129_P164_H0.6441.00
64_R71_D0.6431.00
116_D120_R0.6421.00
189_P193_D0.6421.00
16_E20_A0.6411.00
40_I83_F0.6401.00
108_G145_F0.6271.00
34_A37_E0.6261.00
3_L187_L0.6241.00
126_D160_F0.6201.00
155_R161_D0.6171.00
156_A164_H0.6151.00
193_D196_A0.6141.00
167_I170_L0.6141.00
153_L161_D0.6121.00
46_F203_A0.6111.00
126_D161_D0.6101.00
147_E150_G0.6081.00
6_L79_F0.6081.00
164_H167_I0.6071.00
2_T79_F0.6041.00
201_A204_P0.6031.00
4_D7_R0.6011.00
84_H91_H0.5991.00
126_D170_L0.5931.00
5_F25_Y0.5931.00
106_V199_F0.5851.00
145_F202_L0.5841.00
67_R74_P0.5841.00
186_V189_P0.5841.00
66_V70_K0.5821.00
193_D197_E0.5811.00
32_M80_G0.5801.00
133_R165_P0.5801.00
151_E174_S0.5771.00
156_A159_G0.5761.00
66_V71_D0.5761.00
135_I139_K0.5741.00
120_R124_A0.5741.00
60_F66_V0.5711.00
142_K147_E0.5701.00
124_A208_F0.5681.00
40_I56_K0.5631.00
33_L55_A0.5611.00
52_A55_A0.5601.00
198_A202_L0.5601.00
32_M36_V0.5601.00
7_R10_K0.5571.00
29_R59_L0.5551.00
125_I170_L0.5531.00
53_L56_K0.5531.00
64_R72_K0.5531.00
184_A188_S0.5491.00
185_E189_P0.5471.00
39_L42_E0.5451.00
119_K158_R0.5451.00
181_L185_E0.5431.00
35_L39_L0.5391.00
60_F82_S0.5381.00
97_F199_F0.5371.00
14_N17_W0.5371.00
126_D166_A0.5361.00
155_R167_I0.5331.00
2_T99_I0.5301.00
48_P51_V0.5301.00
142_K146_G0.5301.00
50_I95_Y0.5291.00
44_G52_A0.5281.00
187_L190_D0.5271.00
40_I52_A0.5201.00
184_A189_P0.5191.00
80_G105_F0.5151.00
188_S194_E0.5121.00
36_V57_G0.5101.00
2_T6_L0.5051.00
64_R69_S0.5021.00
202_L206_V0.5021.00
110_I117_Q0.5021.00
70_K73_T0.5011.00
18_F26_E0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2a8eA 1 0.8846 90.5 0.907 Contact Map
3mqzA 1 0.8942 85.4 0.915 Contact Map
3guvA 2 0.2885 9.2 0.954 Contact Map
2debA 1 0.6298 8.4 0.955 Contact Map
3kb4A 2 0.5673 8.1 0.956 Contact Map
3g13A 2 0.3269 7.2 0.957 Contact Map
1wddS 3 0.5144 6.1 0.958 Contact Map
1t1uA 1 0.6298 6 0.958 Contact Map
2jugA 2 0.3125 5.5 0.959 Contact Map
3ijdA 2 0.3317 3.9 0.962 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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