GREMLIN Database
DUF1850 - Domain of unknown function (DUF1850)
PFAM: PF08905 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 86 (86)
Sequences: 4718 (3121)
Seq/√Len: 336.6
META: 0.834

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
33_K53_W3.5301.00
24_G61_P2.7931.00
29_E55_V2.7351.00
19_R28_V2.6941.00
21_T26_V2.5821.00
17_D29_E2.5131.00
31_R53_W2.4111.00
4_S15_E2.2231.00
12_T33_K2.2201.00
6_T13_P2.1291.00
15_E31_R1.9121.00
16_E30_A1.8591.00
18_Y27_L1.8261.00
29_E57_R1.7831.00
69_V74_D1.7021.00
4_S13_P1.6871.00
62_L65_L1.6281.00
10_E39_M1.6201.00
5_W14_V1.6021.00
50_E55_V1.5741.00
17_D31_R1.5611.00
24_G63_P1.5411.00
21_T24_G1.5251.00
76_R83_C1.4821.00
70_S73_A1.4471.00
14_V39_M1.4011.00
30_A40_P1.3451.00
10_E37_A1.3311.00
2_T15_E1.3171.00
72_V75_H1.2421.00
4_S76_R1.2261.00
4_S78_C1.2221.00
2_T17_D1.2081.00
3_L67_L1.2011.00
10_E34_G1.1791.00
26_V59_N1.1761.00
31_R55_V1.1671.00
78_C83_C1.1541.00
12_T34_G1.1401.00
19_R26_V1.1381.00
46_G50_E1.0481.00
44_G47_L1.0441.00
70_S75_H1.0021.00
26_V61_P0.9781.00
10_E14_V0.9671.00
79_I82_R0.9481.00
10_E38_G0.9051.00
14_V70_S0.9031.00
70_S74_D0.8791.00
50_E53_W0.8541.00
13_P76_R0.8441.00
12_T36_G0.8321.00
16_E37_A0.8291.00
76_R85_P0.8271.00
27_L58_P0.8121.00
32_F35_F0.8031.00
42_P45_A0.7941.00
30_A56_I0.7771.00
10_E41_P0.7761.00
81_G84_I0.7741.00
43_P46_G0.7701.00
17_D28_V0.7681.00
1_F25_L0.7491.00
79_I84_I0.7470.99
9_V37_A0.7320.99
10_E40_P0.7230.99
69_V86_L0.7220.99
35_F54_F0.7200.99
30_A39_M0.7160.99
62_L66_V0.7080.99
28_V59_N0.7060.99
20_V79_I0.7060.99
35_F41_P0.7040.99
26_V60_R0.6990.99
43_P47_L0.6960.99
43_P49_L0.6890.99
3_L75_H0.6890.99
12_T38_G0.6790.99
9_V16_E0.6750.99
42_P56_I0.6740.99
15_E53_W0.6570.99
9_V38_G0.6500.99
18_Y25_L0.6500.99
27_L62_L0.6380.98
35_F56_I0.6380.98
5_W37_A0.6260.98
49_L57_R0.6210.98
83_C86_L0.6210.98
6_T74_D0.6190.98
8_S70_S0.6170.98
45_A48_T0.6160.98
1_F79_I0.6160.98
1_F18_Y0.6110.98
10_E54_F0.6060.98
8_S69_V0.6030.98
74_D85_P0.6030.98
1_F69_V0.5990.98
40_P54_F0.5950.98
23_G26_V0.5910.97
5_W39_M0.5910.97
41_P44_G0.5810.97
8_S71_P0.5800.97
19_R24_G0.5800.97
9_V39_M0.5800.97
59_N84_I0.5760.97
20_V77_L0.5630.97
10_E33_K0.5620.97
11_K34_G0.5580.96
12_T40_P0.5560.96
6_T11_K0.5550.96
74_D79_I0.5500.96
12_T39_M0.5490.96
46_G57_R0.5480.96
15_E33_K0.5450.96
32_F41_P0.5440.96
6_T76_R0.5430.96
50_E84_I0.5400.96
36_G40_P0.5350.95
13_P33_K0.5340.95
32_F37_A0.5330.95
80_G84_I0.5310.95
63_P80_G0.5240.95
5_W10_E0.5240.95
43_P48_T0.5170.94
4_S83_C0.5100.94
42_P49_L0.5090.94
30_A58_P0.5090.94
82_R85_P0.5060.94
9_V34_G0.5000.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4qtqA 1 0.6744 28.4 0.864 Contact Map
4rh7A 1 0.7558 15.7 0.88 Contact Map
3vkgA 2 0.7558 14 0.883 Contact Map
4akgA 2 0.7558 11.8 0.886 Contact Map
4m9dA 2 0.593 10.7 0.888 Contact Map
3hidA 2 0.593 7.9 0.895 Contact Map
2du3A 4 0.9767 7.5 0.896 Contact Map
3ecfA 2 0.6047 6.8 0.898 Contact Map
2du7A 7 0.9767 5 0.904 Contact Map
2d7uA 2 0.5814 4.8 0.905 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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