GREMLIN Database
MBF1 - Multiprotein bridging factor 1
PFAM: PF08523 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 72 (72)
Sequences: 854 (583)
Seq/√Len: 68.6
META: 0.509

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
58_A62_R5.2051.00
22_E29_R3.6611.00
26_N29_R2.5631.00
21_S24_A2.3851.00
54_G57_A2.0691.00
1_Q4_D1.9991.00
27_A30_R1.9111.00
42_G45_G1.8911.00
63_E67_L1.8881.00
26_N30_R1.8631.00
1_Q8_V1.8391.00
25_V34_V1.6570.99
21_S25_V1.6480.99
63_E66_E1.5290.99
2_D5_P1.4840.99
25_V35_V1.4820.99
53_E56_N1.4820.99
60_L69_H1.3550.98
68_K71_K1.3460.98
27_A31_S1.3220.97
29_R35_V1.3190.97
56_N62_R1.3170.97
53_E57_A1.3000.97
19_A27_A1.2660.96
7_T11_K1.2600.96
1_Q7_T1.2240.96
23_S27_A1.1870.95
19_A25_V1.1760.95
57_A67_L1.1720.94
31_S36_E1.0600.91
25_V30_R1.0330.90
64_T68_K1.0220.89
66_E69_H1.0190.89
11_K14_P1.0150.89
63_E68_K1.0130.89
55_Q69_H1.0090.89
61_D64_T1.0060.89
41_F44_G0.9830.87
20_K23_S0.9580.86
49_H52_T0.9390.85
43_A47_K0.9270.84
57_A60_L0.9180.84
15_K18_V0.9080.83
18_V24_A0.8950.82
39_K42_G0.8890.81
57_A61_D0.8760.81
14_P18_V0.8570.79
64_T67_L0.8500.79
18_V23_S0.8230.76
1_Q5_P0.8080.75
40_K61_D0.8070.75
10_G14_P0.8020.74
26_N58_A0.7840.73
17_T22_E0.7730.72
24_A36_E0.7670.71
39_K46_N0.7550.70
10_G13_A0.7500.69
25_V36_E0.7460.69
19_A22_E0.7440.69
62_R66_E0.7340.68
23_S29_R0.7320.67
17_T29_R0.7320.67
59_K65_E0.7260.67
20_K61_D0.7250.67
7_T38_E0.7170.66
6_V10_G0.7150.66
10_G20_K0.7020.64
50_S59_K0.6910.63
39_K54_G0.6790.62
40_K46_N0.6690.60
24_A31_S0.6650.60
51_G65_E0.6640.60
9_I13_A0.6500.58
39_K68_K0.6440.58
30_R61_D0.6400.57
36_E45_G0.6390.57
30_R40_K0.6360.57
53_E60_L0.6350.57
26_N55_Q0.6290.56
28_A37_T0.6280.56
40_K71_K0.6100.54
29_R33_A0.6030.53
37_T70_K0.6030.53
23_S58_A0.5980.52
1_Q44_G0.5950.52
16_A40_K0.5890.51
47_K51_G0.5860.51
25_V28_A0.5840.50
68_K72_V0.5590.47
5_P8_V0.5530.47
23_S26_N0.5430.46
26_N34_V0.5350.45
52_T60_L0.5350.45
37_T43_A0.5320.44
9_I28_A0.5310.44
54_G70_K0.5310.44
29_R48_Q0.5280.44
46_N65_E0.5200.43
9_I12_K0.5170.42
56_N60_L0.5170.42
1_Q9_I0.5150.42
1_Q23_S0.5060.41
25_V37_T0.5060.41
31_S56_N0.5040.41
3_W59_K0.5030.41
67_L71_K0.5020.41
1_Q21_S0.5020.41
34_V57_A0.5000.40
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4n6hA 1 0.9444 5 0.893 Contact Map
3he5A 1 0.3611 2.9 0.905 Contact Map
3zx7A 1 0.75 2 0.913 Contact Map
1k04A 2 0.3056 1.4 0.921 Contact Map
3kptA 1 0.375 1.3 0.922 Contact Map
3j20F 1 0.7778 1.2 0.922 Contact Map
4yayA 1 0.9583 1 0.927 Contact Map
3zeyP 1 0.7778 1 0.927 Contact Map
2jvlA 1 0.4306 0.9 0.928 Contact Map
1dugA 2 0.4722 0.9 0.928 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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