GREMLIN Database
DUF1512 - Protein of unknown function (DUF1512)
PFAM: PF07431 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 356 (352)
Sequences: 857 (559)
Seq/√Len: 29.8
META: 0.93

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
190_A222_V5.9891.00
216_E335_R5.3171.00
203_E226_E4.9821.00
184_G355_V4.0861.00
338_T342_D3.9541.00
215_I220_L3.9331.00
46_R66_D3.8311.00
317_K321_D3.6081.00
212_E219_K3.5881.00
111_D114_E3.5841.00
253_I292_A3.5551.00
39_K43_D3.4301.00
280_G283_V3.3821.00
185_I322_A3.3401.00
239_V347_A3.1361.00
252_R300_Y3.1311.00
120_N230_G2.9971.00
316_R319_I2.9211.00
255_M276_A2.8761.00
93_D234_K2.8061.00
193_L346_V2.6901.00
256_I346_V2.6141.00
208_T223_L2.5240.99
104_K115_V2.5150.99
79_L85_V2.3990.99
221_Y343_T2.3930.99
185_I304_I2.3880.99
215_I335_R2.3050.99
339_K342_D2.2890.99
194_M211_A2.1920.98
192_K324_D2.1730.98
239_V345_I2.0890.98
302_I326_V2.0840.98
77_V85_V2.0680.98
89_E93_D2.0620.98
136_R264_G2.0020.97
332_R336_E1.9890.97
188_L355_V1.9680.97
267_T310_E1.9650.97
92_L281_P1.9410.96
267_T316_R1.9160.96
68_F122_L1.8980.96
322_A326_V1.8970.96
255_M288_I1.8920.96
207_D231_T1.8700.96
220_L346_V1.8630.95
276_A288_I1.8590.95
129_N133_K1.8590.95
95_R123_E1.8360.95
80_D83_G1.8160.95
87_K280_G1.7770.94
65_V122_L1.7740.94
75_E78_D1.7430.93
157_I161_I1.7120.93
302_I330_V1.7120.93
260_L311_A1.7070.93
20_Q23_Q1.7010.93
243_I253_I1.6700.92
85_V280_G1.6380.91
216_E331_K1.6340.91
219_K343_T1.6300.91
185_I326_V1.6200.91
70_E129_N1.6100.90
194_M222_V1.6010.90
85_V286_F1.5950.90
205_A237_E1.5530.89
266_K318_E1.5410.88
213_V222_V1.5410.88
259_A271_A1.5390.88
79_L84_I1.5380.88
38_L165_A1.5340.88
100_K206_K1.5310.88
210_V221_Y1.5180.87
79_L88_L1.5070.87
39_K136_R1.4900.86
326_V330_V1.4790.86
72_F303_V1.4790.86
328_E331_K1.4750.86
270_V323_V1.4750.86
304_I326_V1.4680.86
112_E226_E1.4580.85
221_Y246_N1.4470.85
4_Q11_F1.4360.84
69_L125_A1.4300.84
330_V346_V1.3890.82
217_G340_E1.3860.82
232_V235_P1.3770.82
77_V236_G1.3740.82
104_K112_E1.3560.81
77_V286_F1.3530.81
32_E143_K1.3520.81
73_T91_I1.3440.80
223_L242_L1.3400.80
175_F181_I1.3340.80
35_L132_Y1.3290.79
87_K283_V1.3240.79
193_L215_I1.3210.79
184_G353_I1.3200.79
89_E287_K1.3180.79
237_E287_K1.2850.77
214_E219_K1.2810.77
117_N229_G1.2670.76
185_I323_V1.2670.76
256_I302_I1.2650.76
325_K328_E1.2620.76
49_A125_A1.2590.75
80_D290_E1.2530.75
85_V91_I1.2520.75
97_D206_K1.2460.75
286_F290_E1.2410.74
85_V236_G1.2320.74
127_A168_Y1.2270.74
302_I333_I1.2200.73
5_L9_L1.2100.72
185_I319_I1.1960.72
292_A299_L1.1920.71
88_L281_P1.1890.71
15_F19_G1.1780.70
107_A118_L1.1760.70
77_V285_K1.1730.70
270_V302_I1.1710.70
256_I330_V1.1670.70
37_K166_E1.1670.70
47_K51_K1.1640.69
193_L222_V1.1600.69
142_G356_G1.1590.69
210_V242_L1.1580.69
103_V178_G1.1540.69
239_V349_V1.1480.68
174_A314_P1.1430.68
2_I7_W1.1360.68
205_A210_V1.1330.67
120_N231_T1.1260.67
74_I277_A1.1180.66
151_I355_V1.1170.66
185_I189_V1.1160.66
245_E248_G1.1070.66
149_Y153_Q1.0990.65
50_I62_T1.0940.65
121_L176_K1.0940.65
116_S231_T1.0860.64
243_I297_I1.0850.64
149_Y156_M1.0690.63
315_M319_I1.0680.63
8_I11_F1.0640.62
217_G220_L1.0610.62
83_G290_E1.0560.62
276_A283_V1.0510.61
142_G153_Q1.0450.61
131_I165_A1.0350.60
168_Y281_P1.0240.59
148_L152_M1.0190.59
130_Q134_I1.0140.59
141_L163_E1.0120.58
255_M308_I1.0070.58
84_I87_K1.0050.58
163_E309_E1.0010.58
243_I250_I0.9990.57
136_R140_L0.9960.57
83_G286_F0.9920.57
71_Y300_Y0.9900.57
89_E237_E0.9850.56
113_V231_T0.9840.56
92_L97_D0.9790.56
77_V292_A0.9780.56
57_G61_P0.9780.56
52_Y118_L0.9740.56
164_E255_M0.9670.55
100_K116_S0.9670.55
158_L162_M0.9630.55
270_V325_K0.9540.54
332_R335_R0.9520.54
212_E246_N0.9520.54
85_V283_V0.9510.54
7_W14_F0.9430.53
208_T260_L0.9390.53
63_K66_D0.9390.53
306_M310_E0.9360.53
249_K291_V0.9310.52
173_K256_I0.9230.52
214_E217_G0.9230.52
184_G188_L0.9150.51
290_E294_K0.9140.51
123_E235_P0.9090.50
223_L239_V0.9080.50
271_A305_K0.9050.50
131_I175_F0.9030.50
251_A345_I0.8980.50
134_I141_L0.8960.49
236_G281_P0.8940.49
117_N178_G0.8930.49
6_I13_L0.8900.49
198_E340_E0.8870.49
133_K271_A0.8860.49
87_K290_E0.8840.48
350_G354_G0.8790.48
348_G354_G0.8790.48
142_G267_T0.8780.48
42_R139_Y0.8770.48
117_N353_I0.8760.48
330_V355_V0.8740.48
338_T344_V0.8730.48
134_I138_Y0.8720.47
85_V89_E0.8660.47
35_L139_Y0.8640.47
278_I285_K0.8620.47
343_T346_V0.8540.46
88_L242_L0.8510.46
133_K224_K0.8450.45
41_M146_K0.8430.45
204_I242_L0.8390.45
138_Y262_L0.8360.45
80_D286_F0.8340.45
79_L87_K0.8340.45
321_D325_K0.8330.44
87_K281_P0.8210.43
187_P304_I0.8210.43
348_G351_N0.8180.43
133_K305_K0.8170.43
124_A266_K0.8160.43
351_N354_G0.8160.43
223_L347_A0.8160.43
204_I238_A0.8150.43
45_A172_L0.8140.43
278_I349_V0.8140.43
284_E352_T0.8100.43
334_I344_V0.8020.42
98_R125_A0.8020.42
252_R333_I0.8020.42
281_P307_S0.7980.42
11_F21_R0.7970.42
218_R338_T0.7950.41
235_P349_V0.7940.41
325_K329_R0.7940.41
327_I331_K0.7880.41
187_P278_I0.7880.41
122_L302_I0.7860.41
250_I343_T0.7860.41
100_K251_A0.7840.41
259_A305_K0.7830.41
120_N171_A0.7830.41
103_V170_K0.7750.40
125_A230_G0.7710.40
310_E316_R0.7690.39
299_L335_R0.7690.39
109_N133_K0.7680.39
172_L312_I0.7680.39
20_Q132_Y0.7620.39
15_F256_I0.7610.39
236_G286_F0.7590.39
21_R25_Y0.7580.39
71_Y252_R0.7570.39
14_F143_K0.7540.38
220_L334_I0.7500.38
132_Y140_L0.7490.38
135_V154_L0.7450.38
120_N280_G0.7430.38
251_A342_D0.7430.38
133_K355_V0.7430.38
236_G284_E0.7420.37
126_M276_A0.7400.37
89_E236_G0.7380.37
80_D143_K0.7370.37
163_E307_S0.7290.37
120_N286_F0.7270.36
223_L345_I0.7270.36
55_K61_P0.7270.36
240_E256_I0.7260.36
240_E244_E0.7260.36
27_M148_L0.7240.36
147_N352_T0.7230.36
96_E99_F0.7230.36
11_F133_K0.7220.36
244_E294_K0.7210.36
86_K256_I0.7210.36
68_F141_L0.7200.36
140_L264_G0.7170.36
2_I14_F0.7170.36
346_V355_V0.7170.36
256_I331_K0.7160.36
123_E232_V0.7150.35
139_Y179_Q0.7140.35
190_A193_L0.7130.35
87_K285_K0.7120.35
101_D104_K0.7120.35
5_L15_F0.7110.35
88_L285_K0.7110.35
226_E356_G0.7100.35
194_M224_K0.7090.35
331_K355_V0.7090.35
131_I240_E0.7090.35
143_K205_A0.7070.35
112_E324_D0.7070.35
31_I142_G0.7070.35
69_L90_H0.7060.35
307_S310_E0.7010.34
224_K318_E0.6990.34
70_E133_K0.6980.34
189_V256_I0.6980.34
193_L334_I0.6970.34
35_L135_V0.6920.34
217_G270_V0.6920.34
5_L8_I0.6900.34
133_K265_E0.6900.34
329_R332_R0.6860.33
35_L153_Q0.6850.33
26_I151_I0.6840.33
5_L14_F0.6830.33
212_E221_Y0.6830.33
44_E244_E0.6810.33
53_I62_T0.6800.33
71_Y274_V0.6790.33
123_E175_F0.6770.33
66_D339_K0.6760.33
29_R166_E0.6750.33
178_G294_K0.6740.33
111_D335_R0.6730.33
9_L39_K0.6720.32
30_E155_Q0.6710.32
132_Y153_Q0.6700.32
124_A172_L0.6700.32
292_A297_I0.6680.32
136_R262_L0.6670.32
95_R276_A0.6660.32
87_K120_N0.6660.32
185_I306_M0.6650.32
252_R274_V0.6650.32
20_Q149_Y0.6640.32
135_V157_I0.6640.32
259_A303_V0.6640.32
44_E47_K0.6610.32
149_Y152_M0.6600.32
121_L175_F0.6600.32
64_R67_R0.6600.32
248_G295_Y0.6570.31
89_E136_R0.6540.31
99_F234_K0.6520.31
187_P190_A0.6500.31
71_Y164_E0.6500.31
27_M144_K0.6460.31
60_D63_K0.6400.30
200_K212_E0.6380.30
20_Q140_L0.6370.30
297_I326_V0.6360.30
28_L139_Y0.6350.30
254_I302_I0.6350.30
181_I211_A0.6350.30
34_S49_A0.6350.30
154_L321_D0.6340.30
175_F353_I0.6340.30
261_K264_G0.6320.30
259_A270_V0.6300.30
68_F300_Y0.6270.29
127_A130_Q0.6270.29
7_W24_L0.6260.29
204_I346_V0.6250.29
9_L13_L0.6180.29
12_I15_F0.6180.29
79_L286_F0.6180.29
5_L239_V0.6170.29
216_E328_E0.6170.29
14_F22_I0.6160.29
143_K264_G0.6150.29
161_I278_I0.6150.29
4_Q8_I0.6140.29
48_E176_K0.6130.28
209_V217_G0.6130.28
173_K179_Q0.6120.28
146_K160_L0.6110.28
143_K148_L0.6110.28
10_F14_F0.6110.28
125_A346_V0.6080.28
27_M31_I0.6070.28
205_A238_A0.6070.28
187_P355_V0.6070.28
109_N115_V0.6050.28
84_I282_G0.6030.28
277_A301_A0.6020.28
10_F13_L0.6010.28
35_L39_K0.6000.28
117_N158_L0.6000.28
112_E209_V0.5990.28
321_D332_R0.5980.28
9_L22_I0.5970.27
241_K323_V0.5950.27
39_K154_L0.5950.27
124_A127_A0.5940.27
140_L223_L0.5930.27
173_K251_A0.5930.27
213_V331_K0.5920.27
173_K177_K0.5910.27
135_V284_E0.5910.27
152_M322_A0.5900.27
3_L11_F0.5900.27
155_Q280_G0.5900.27
50_I61_P0.5900.27
77_V225_A0.5900.27
192_K323_V0.5880.27
107_A110_A0.5870.27
102_E139_Y0.5860.27
93_D99_F0.5850.27
205_A208_T0.5850.27
12_I21_R0.5850.27
215_I327_I0.5850.27
267_T307_S0.5840.27
139_Y164_E0.5820.27
230_G353_I0.5820.27
103_V206_K0.5820.27
267_T312_I0.5820.27
27_M149_Y0.5810.26
118_L356_G0.5810.26
71_Y139_Y0.5810.26
327_I330_V0.5800.26
322_A343_T0.5800.26
163_E187_P0.5800.26
294_K352_T0.5790.26
50_I65_V0.5790.26
19_G139_Y0.5780.26
11_F299_L0.5780.26
159_P265_E0.5780.26
337_R342_D0.5770.26
234_K287_K0.5760.26
87_K286_F0.5760.26
8_I12_I0.5730.26
68_F99_F0.5720.26
26_I30_E0.5710.26
86_K163_E0.5710.26
114_E310_E0.5710.26
117_N230_G0.5700.26
300_Y309_E0.5700.26
330_V334_I0.5700.26
181_I353_I0.5700.26
139_Y153_Q0.5690.26
161_I188_L0.5690.26
98_R145_T0.5690.26
13_L154_L0.5680.26
74_I276_A0.5680.26
302_I322_A0.5670.26
168_Y234_K0.5650.25
166_E200_K0.5650.25
291_V295_Y0.5650.25
77_V87_K0.5650.25
21_R262_L0.5640.25
292_A325_K0.5640.25
224_K305_K0.5610.25
74_I285_K0.5610.25
175_F259_A0.5600.25
260_L307_S0.5590.25
178_G344_V0.5580.25
89_E145_T0.5560.25
45_A174_A0.5550.25
57_G108_P0.5550.25
96_E119_E0.5550.25
95_R124_A0.5550.25
314_P356_G0.5540.25
95_R175_F0.5540.25
270_V322_A0.5540.25
220_L330_V0.5520.25
331_K335_R0.5510.25
254_I330_V0.5510.25
197_K211_A0.5490.25
65_V109_N0.5480.24
188_L195_L0.5480.24
239_V288_I0.5470.24
142_G151_I0.5470.24
12_I58_K0.5460.24
149_Y232_V0.5460.24
127_A232_V0.5460.24
333_I336_E0.5460.24
95_R119_E0.5440.24
133_K221_Y0.5430.24
96_E239_V0.5420.24
39_K42_R0.5420.24
187_P216_E0.5410.24
204_I356_G0.5410.24
307_S311_A0.5400.24
119_E276_A0.5400.24
199_K256_I0.5400.24
308_I318_E0.5390.24
89_E303_V0.5390.24
253_I259_A0.5370.24
65_V97_D0.5370.24
122_L126_M0.5360.24
41_M266_K0.5350.24
187_P224_K0.5350.24
300_Y337_R0.5340.24
90_H150_L0.5340.24
139_Y300_Y0.5330.24
320_A326_V0.5310.24
89_E276_A0.5310.24
44_E108_P0.5300.23
322_A325_K0.5250.23
53_I61_P0.5240.23
147_N156_M0.5230.23
144_K316_R0.5230.23
242_L262_L0.5230.23
84_I301_A0.5230.23
190_A269_E0.5230.23
83_G143_K0.5220.23
16_Y19_G0.5220.23
87_K282_G0.5190.23
348_G352_T0.5190.23
280_G288_I0.5180.23
334_I338_T0.5180.23
107_A341_G0.5170.23
80_D280_G0.5160.23
14_F191_A0.5150.23
190_A256_I0.5150.23
133_K319_I0.5130.23
341_G346_V0.5130.23
35_L136_R0.5120.22
166_E250_I0.5120.22
212_E245_E0.5120.22
108_P230_G0.5110.22
5_L320_A0.5100.22
181_I236_G0.5100.22
32_E70_E0.5090.22
95_R145_T0.5090.22
276_A349_V0.5080.22
74_I109_N0.5080.22
13_L92_L0.5070.22
54_K92_L0.5070.22
17_G20_Q0.5040.22
223_L285_K0.5020.22
271_A312_I0.5010.22
23_Q123_E0.5010.22
187_P294_K0.5010.22
37_K255_M0.5000.22
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4s12A 2 0.2022 37 0.976 Contact Map
1x92A 4 0.1629 30.3 0.977 Contact Map
3w0lB 1 0.2022 28.3 0.978 Contact Map
2e85A 2 0.382 27.5 0.978 Contact Map
3trjA 4 0.1545 26.6 0.978 Contact Map
2cxcA 1 0.3287 24.8 0.978 Contact Map
4bb9A 1 0.236 20.5 0.979 Contact Map
3pu6A 1 0.3343 19.9 0.979 Contact Map
2i2wA 4 0.1517 19.3 0.979 Contact Map
1c8bA 3 0.6236 15.5 0.98 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0111 seconds.