GREMLIN Database
DUF1444 - Protein of unknown function (DUF1444)
PFAM: PF07285 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 264 (262)
Sequences: 566 (422)
Seq/√Len: 26.1
META: 0.468

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
202_V214_A5.2841.00
198_E217_R4.5461.00
109_D146_R4.5371.00
231_K235_E4.2011.00
3_S28_D3.8191.00
202_V225_L3.7221.00
201_M213_I3.6581.00
9_K12_E3.6261.00
8_K11_E3.3361.00
6_M49_Y3.2351.00
204_A229_T3.2041.00
2_T5_K3.1491.00
169_D217_R3.0351.00
4_K7_K2.9511.00
7_K11_E2.8701.00
197_V201_M2.7320.99
177_D183_R2.6900.99
29_T42_T2.5600.99
5_K12_E2.5320.99
164_E169_D2.3610.98
119_Y146_R2.3590.98
107_V135_L2.3300.98
173_L189_F2.2540.98
122_D179_Y2.2400.98
5_K9_K2.1490.97
21_F30_L2.1220.97
162_K169_D1.9260.94
258_L262_K1.8530.93
204_A259_A1.8240.93
151_F154_R1.8040.92
30_L63_V1.7890.92
60_I64_V1.7750.92
98_T116_R1.7710.91
2_T28_D1.7150.90
4_K8_K1.7030.90
23_F49_Y1.6870.89
177_D180_D1.6680.89
28_D46_P1.6150.87
133_K136_E1.6140.87
82_G138_E1.5990.87
189_F213_I1.5790.86
153_L197_V1.5690.85
16_K19_R1.5600.85
244_S259_A1.5590.85
11_E25_R1.5460.85
147_E151_F1.5110.83
202_V246_L1.5060.83
58_K251_E1.5030.83
11_E15_K1.4860.82
4_K22_S1.4790.82
200_D246_L1.4600.81
132_E136_E1.4570.81
49_Y52_Y1.4460.80
31_R40_G1.4430.80
92_R181_A1.4280.80
135_L145_L1.4260.79
241_T245_F1.4120.79
209_D236_G1.4020.78
21_F29_T1.3820.77
178_G183_R1.3700.77
168_N218_N1.3670.76
153_L262_K1.3600.76
246_L259_A1.3320.74
48_I63_V1.3210.74
62_E66_Y1.3180.74
85_K155_S1.3080.73
241_T247_Y1.3000.73
84_E138_E1.2560.70
114_E245_F1.2520.70
153_L158_T1.2460.69
4_K26_E1.2440.69
190_L201_M1.2350.69
9_K13_R1.2350.69
3_S262_K1.2310.68
251_E258_L1.2280.68
2_T7_K1.2280.68
18_D35_K1.2270.68
92_R129_L1.2250.68
61_D64_V1.2140.67
184_I203_V1.2130.67
7_K19_R1.2120.67
3_S6_M1.2120.67
73_A78_Q1.2080.67
220_T261_N1.1790.65
14_L30_L1.1670.64
83_D138_E1.1630.64
96_F207_H1.1510.63
7_K21_F1.1420.62
110_E135_L1.1410.62
6_M9_K1.1360.62
34_H75_E1.1340.62
3_S46_P1.1220.61
164_E167_G1.1190.61
87_I119_Y1.1060.60
197_V262_K1.0950.59
12_E15_K1.0950.59
2_T19_R1.0920.59
80_L138_E1.0900.59
30_L47_N1.0890.59
119_Y145_L1.0830.58
34_H37_T1.0740.58
28_D49_Y1.0720.57
218_N225_L1.0710.57
11_E21_F1.0700.57
22_S35_K1.0690.57
207_H232_F1.0670.57
141_T144_E1.0610.57
121_L157_P1.0600.57
17_E35_K1.0430.55
215_D222_Y1.0410.55
205_V247_Y1.0410.55
197_V215_D1.0410.55
15_K24_D1.0320.55
8_K20_T1.0320.55
108_Y116_R1.0310.54
184_I205_V1.0290.54
140_W144_E1.0260.54
99_E103_G1.0230.54
5_K200_D1.0210.54
80_L140_W1.0190.54
34_H259_A1.0100.53
80_L83_D1.0020.52
92_R115_T1.0000.52
160_V173_L0.9980.52
12_E20_T0.9770.51
52_Y56_K0.9770.51
158_T173_L0.9760.50
90_V168_N0.9750.50
9_K23_F0.9750.50
24_D27_K0.9730.50
181_A209_D0.9720.50
3_S49_Y0.9670.50
259_A262_K0.9570.49
143_E148_I0.9550.49
111_H210_V0.9550.49
246_L251_E0.9510.49
120_A156_L0.9470.48
18_D25_R0.9430.48
3_S7_K0.9400.48
7_K18_D0.9380.48
120_A129_L0.9330.47
2_T53_E0.9270.47
5_K13_R0.9110.46
19_R24_D0.9090.45
147_E154_R0.9030.45
94_T116_R0.8850.44
108_Y184_I0.8830.44
91_I94_T0.8810.43
183_R211_L0.8750.43
120_A187_E0.8730.43
15_K18_D0.8720.43
44_S47_N0.8720.43
47_N258_L0.8710.43
11_E19_R0.8670.42
117_I185_L0.8650.42
21_F25_R0.8550.42
11_E22_S0.8540.41
90_V207_H0.8530.41
111_H114_E0.8520.41
19_R34_H0.8490.41
6_M47_N0.8480.41
82_G183_R0.8480.41
60_I67_V0.8450.41
44_S52_Y0.8440.41
19_R75_E0.8420.41
65_Y69_E0.8370.40
90_V221_G0.8360.40
129_L208_Q0.8330.40
95_S172_F0.8310.40
5_K8_K0.8300.40
9_K15_K0.8250.39
41_V70_A0.8250.39
10_L64_V0.8230.39
33_E38_G0.8150.39
29_T262_K0.8110.38
84_E155_S0.8110.38
106_L259_A0.8110.38
60_I63_V0.8090.38
237_R258_L0.8070.38
200_D259_A0.8070.38
132_E142_E0.8030.38
73_A126_S0.8030.38
36_E139_G0.8020.38
178_G212_I0.7980.38
189_F193_M0.7970.37
17_E164_E0.7960.37
176_N180_D0.7940.37
119_Y204_A0.7910.37
15_K139_G0.7870.37
11_E30_L0.7860.37
64_V68_E0.7850.37
199_G202_V0.7850.37
204_A214_A0.7850.37
193_M203_V0.7830.37
22_S173_L0.7800.36
100_T175_A0.7780.36
8_K21_F0.7700.36
23_F198_E0.7670.35
228_M232_F0.7650.35
92_R112_T0.7650.35
11_E26_E0.7600.35
150_L153_L0.7590.35
95_S223_D0.7560.35
35_K133_K0.7540.35
128_R261_N0.7520.34
64_V67_V0.7520.34
117_I218_N0.7500.34
129_L238_V0.7500.34
48_I261_N0.7470.34
19_R38_G0.7450.34
83_D88_F0.7430.34
2_T16_K0.7400.34
144_E258_L0.7400.34
46_P49_Y0.7360.33
6_M153_L0.7350.33
159_E196_K0.7320.33
87_I182_S0.7320.33
87_I130_I0.7290.33
194_E246_L0.7280.33
2_T259_A0.7280.33
38_G262_K0.7270.33
16_K35_K0.7220.32
156_L211_L0.7180.32
26_E175_A0.7170.32
2_T37_T0.7150.32
181_A221_G0.7150.32
197_V259_A0.7140.32
104_K133_K0.7120.32
16_K38_G0.7110.32
7_K145_L0.7110.32
67_V77_E0.7080.32
226_A242_A0.7080.32
233_F241_T0.7070.31
10_L19_R0.7060.31
194_E249_D0.7000.31
174_N211_L0.7000.31
18_D262_K0.6980.31
226_A259_A0.6970.31
182_S199_G0.6970.31
65_Y73_A0.6910.30
112_T115_T0.6900.30
190_L226_A0.6890.30
66_Y259_A0.6890.30
1_M6_M0.6890.30
17_E262_K0.6890.30
102_E132_E0.6870.30
89_P177_D0.6870.30
119_Y185_L0.6840.30
66_Y174_N0.6840.30
69_E125_K0.6840.30
214_A219_E0.6830.30
92_R172_F0.6810.30
223_D227_Q0.6780.30
93_S126_S0.6770.30
4_K25_R0.6770.30
3_S47_N0.6750.29
20_T27_K0.6710.29
132_E204_A0.6700.29
112_T177_D0.6700.29
246_L250_G0.6690.29
111_H232_F0.6680.29
208_Q211_L0.6670.29
26_E41_V0.6650.29
222_Y227_Q0.6640.29
26_E30_L0.6640.29
13_R61_D0.6620.29
22_S26_E0.6600.29
122_D171_Y0.6590.28
114_E223_D0.6580.28
126_S154_R0.6520.28
92_R177_D0.6520.28
154_R188_S0.6510.28
111_H115_T0.6510.28
9_K18_D0.6510.28
21_F260_K0.6490.28
3_S36_E0.6470.28
11_E14_L0.6440.28
117_I205_V0.6440.28
151_F188_S0.6410.27
6_M259_A0.6410.27
73_A123_L0.6400.27
148_I153_L0.6390.27
12_E22_S0.6390.27
104_K135_L0.6390.27
201_M220_T0.6390.27
7_K15_K0.6350.27
9_K20_T0.6350.27
146_R258_L0.6350.27
146_R238_V0.6290.27
23_F28_D0.6230.26
80_L255_I0.6210.26
10_L34_H0.6210.26
6_M10_L0.6190.26
110_E116_R0.6180.26
131_D147_E0.6180.26
8_K17_E0.6170.26
2_T63_V0.6130.26
53_E97_P0.6120.26
111_H205_V0.6100.26
207_H210_V0.6090.26
113_A170_F0.6070.25
183_R190_L0.6060.25
96_F127_Y0.6030.25
52_Y140_W0.6030.25
5_K17_E0.6010.25
87_I145_L0.6010.25
53_E258_L0.6010.25
4_K11_E0.5980.25
119_Y150_L0.5930.25
189_F201_M0.5890.24
167_G241_T0.5880.24
6_M16_K0.5860.24
168_N221_G0.5840.24
4_K78_Q0.5830.24
164_E198_E0.5830.24
24_D166_A0.5820.24
41_V125_K0.5780.24
7_K22_S0.5770.24
4_K17_E0.5760.24
130_I212_I0.5750.24
112_T172_F0.5750.24
7_K17_E0.5740.24
88_F120_A0.5740.24
235_E250_G0.5720.24
209_D212_I0.5710.23
111_H179_Y0.5700.23
191_K194_E0.5700.23
48_I130_I0.5690.23
8_K18_D0.5670.23
76_E198_E0.5650.23
81_E196_K0.5650.23
37_T73_A0.5640.23
181_A227_Q0.5640.23
38_G51_K0.5630.23
127_Y178_G0.5630.23
9_K98_T0.5620.23
8_K63_V0.5610.23
251_E260_K0.5600.23
144_E148_I0.5580.23
204_A240_I0.5570.23
12_E77_E0.5560.23
21_F26_E0.5550.23
128_R230_M0.5540.23
120_A179_Y0.5520.22
230_M242_A0.5520.22
218_N223_D0.5510.22
92_R183_R0.5490.22
39_K43_I0.5480.22
96_F129_L0.5460.22
7_K45_L0.5440.22
239_P245_F0.5420.22
74_M103_G0.5410.22
60_I66_Y0.5400.22
151_F155_S0.5390.22
91_I185_L0.5390.22
3_S44_S0.5390.22
12_E153_L0.5380.22
11_E17_E0.5370.22
197_V261_N0.5360.22
210_V232_F0.5360.22
22_S139_G0.5350.22
4_K27_K0.5340.22
17_E162_K0.5330.22
230_M234_A0.5310.21
35_K197_V0.5280.21
4_K21_F0.5280.21
9_K16_K0.5270.21
10_L26_E0.5260.21
66_Y70_A0.5250.21
69_E203_V0.5250.21
27_K46_P0.5240.21
146_R187_E0.5230.21
227_Q242_A0.5230.21
88_F124_G0.5220.21
233_F239_P0.5210.21
128_R134_M0.5210.21
95_S207_H0.5200.21
15_K104_K0.5180.21
4_K258_L0.5170.21
16_K21_F0.5150.21
86_R145_L0.5150.21
16_K22_S0.5130.21
2_T6_M0.5120.20
106_L244_S0.5120.20
11_E213_I0.5100.20
117_I132_E0.5100.20
231_K242_A0.5100.20
232_F239_P0.5090.20
4_K48_I0.5060.20
189_F244_S0.5040.20
21_F259_A0.5020.20
93_S240_I0.5010.20
2_T15_K0.5000.20
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3q4fC 2 0.3485 29.3 0.957 Contact Map
4pfuA 2 0.2197 14.2 0.962 Contact Map
4pfyA 1 0.2159 13.4 0.963 Contact Map
4uqwA 1 0.5492 9.6 0.965 Contact Map
1x4pA 1 0.1288 9.5 0.965 Contact Map
3czqA 3 0.7689 9.3 0.965 Contact Map
3vwbA 1 0.3409 7.7 0.967 Contact Map
3vbhD 3 0.0833 7.7 0.967 Contact Map
3g65A 1 0.8258 7.4 0.967 Contact Map
2x4kA 4 0.2348 6 0.968 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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