GREMLIN Database
DUF1427 - Protein of unknown function (DUF1427)
PFAM: PF07235 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 83 (80)
Sequences: 1187 (684)
Seq/√Len: 76.5
META: 0.775

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
2_P30_I4.3891.00
33_V38_I4.2741.00
9_A20_L4.2681.00
40_V70_V2.5971.00
4_L8_G2.5181.00
9_A16_I2.3951.00
45_V48_A2.3921.00
5_L9_A1.9371.00
69_H74_L1.9271.00
61_W68_P1.8521.00
13_V23_R1.6881.00
5_L20_L1.5900.99
73_E76_G1.5320.99
47_L57_L1.4910.99
5_L8_G1.4740.99
5_L18_S1.4660.99
39_L43_Q1.4440.99
71_F74_L1.4420.99
52_L55_E1.3800.99
15_V44_I1.3630.98
12_L16_I1.3490.98
49_K53_A1.3200.98
18_S45_V1.3150.98
66_C70_V1.2530.97
56_P78_H1.2060.97
48_A57_L1.1870.96
61_W71_F1.1830.96
3_Y23_R1.1750.96
23_R39_L1.1400.95
62_L65_Q1.1340.95
6_S15_V1.1280.95
70_V73_E1.1240.95
50_R58_A1.1190.95
50_R66_C1.0980.94
29_V71_F1.0960.94
27_P41_G1.0640.93
63_R67_A1.0190.92
54_G64_S1.0020.91
76_G79_A0.9910.90
70_V77_R0.9800.90
61_W66_C0.9710.90
51_L54_G0.9630.89
9_A22_V0.9610.89
19_L49_K0.9580.89
53_A59_A0.9430.88
8_G15_V0.9350.88
2_P6_S0.9320.88
28_P31_A0.8950.85
57_L66_C0.8940.85
6_S9_A0.8740.84
40_V44_I0.8710.84
59_A63_R0.8180.80
2_P5_L0.8110.79
39_L72_G0.8000.78
34_G41_G0.7960.78
36_L76_G0.7930.78
44_I68_P0.7890.77
55_E67_A0.7770.76
28_P35_L0.7690.75
47_L52_L0.7690.75
60_A76_G0.7680.75
57_L62_L0.7660.75
53_A57_L0.7590.74
74_L77_R0.7580.74
11_L44_I0.7530.74
45_V66_C0.7460.73
22_V33_V0.7430.73
21_N49_K0.7240.71
43_Q72_G0.7190.70
23_R43_Q0.7050.69
16_I46_P0.6990.68
16_I20_L0.6880.67
2_P9_A0.6870.67
24_S41_G0.6760.66
19_L36_L0.6740.65
2_P8_G0.6720.65
63_R70_V0.6720.65
63_R71_F0.6560.63
4_L68_P0.6490.63
1_K71_F0.6360.61
30_I73_E0.6250.60
69_H73_E0.6230.59
57_L67_A0.6220.59
51_L78_H0.6210.59
66_C72_G0.6130.58
62_L68_P0.6090.58
26_A32_L0.6060.57
21_N65_Q0.6010.57
27_P34_G0.5960.56
9_A12_L0.5940.56
7_L10_G0.5900.55
50_R53_A0.5870.55
53_A79_A0.5840.55
4_L48_A0.5830.54
68_P77_R0.5760.54
29_V38_I0.5620.52
73_E77_R0.5580.51
11_L40_V0.5560.51
48_A51_L0.5530.51
56_P63_R0.5520.50
28_P41_G0.5410.49
11_L20_L0.5400.49
26_A31_A0.5400.49
28_P32_L0.5390.49
8_G13_V0.5390.49
57_L61_W0.5380.49
17_Y24_S0.5330.48
73_E78_H0.5320.48
65_Q68_P0.5310.48
1_K21_N0.5220.47
32_L41_G0.5190.46
68_P76_G0.5090.45
38_I69_H0.5090.45
26_A41_G0.5080.45
69_H75_P0.5060.45
15_V79_A0.5050.45
31_A35_L0.5040.44
47_L66_C0.5030.44
21_N52_L0.5010.44
59_A64_S0.5000.44
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2xq2A 2 0.5904 5.8 0.927 Contact Map
1ciiA 1 0.6145 5.5 0.928 Contact Map
3j61O 1 0.3976 4.3 0.932 Contact Map
4njnA 4 0.5904 3.8 0.933 Contact Map
4dx5A 3 0.5301 3.3 0.935 Contact Map
1rh1A 1 0.6386 2.8 0.938 Contact Map
3s0xA 1 0.3133 2.7 0.938 Contact Map
3kxsF 2 0.3373 2.6 0.939 Contact Map
3t33A 2 0.4096 2.6 0.939 Contact Map
2lk9A 1 0.2289 2.6 0.939 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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