FliD_C - PFAM28P - GREMLIN Contacts

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FliD_C - Flagellar hook-associated protein 2 C-terminus

PFAM:	PF07195	Sequences: AQDAKFTVNGITITSSSNTITDAIPGVTLTLKKATPGGTPVTLTVSRDTDAIKDAIKSFVDAYNALISTINSLTKYSEDEIEDEETAKAGPLAGDSTLRSLQSQLRSALSSSVGSGSYSSLSDIGITTDRDTGTLSLDETKLDAALASNPDAVAALFTGDGTGDGTVTSTTGLATRLDDLLDSYTDSGTGLLTTRTDSLNSQIKDLDDQIDDLEDRLEALEARLRAQFTAMETALSQLNS	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_10
Length:	240 (224)
Sequences:	16530 (13396)
Seq/√Len:	895.0
META:	0.807

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
68_S	134_T	3.327	1.00
53_K	143_D	3.151	1.00
5_K	12_T	2.972	1.00
149_N	152_A	2.915	1.00
221_E	225_R	2.746	1.00
57_K	139_E	2.589	1.00
7_T	12_T	2.498	1.00
3_D	14_T	2.467	1.00
53_K	139_E	2.434	1.00
57_K	61_D	2.320	1.00
129_D	136_S	2.272	1.00
220_L	223_R	2.190	1.00
5_K	14_T	2.177	1.00
69_T	73_L	2.146	1.00
15_S	22_D	2.016	1.00
54_D	58_S	2.006	1.00
222_A	226_A	1.935	1.00
226_A	230_A	1.918	1.00
129_D	134_T	1.910	1.00
3_D	16_S	1.869	1.00
68_S	72_S	1.852	1.00
61_D	65_A	1.848	1.00
6_F	20_I	1.811	1.00
19_T	30_T	1.779	1.00
144_A	148_S	1.778	1.00
137_L	142_L	1.729	1.00
20_I	31_L	1.713	1.00
124_I	156_L	1.704	1.00
6_F	13_I	1.686	1.00
214_E	218_E	1.685	1.00
215_D	219_A	1.666	1.00
143_D	147_A	1.664	1.00
53_K	57_K	1.661	1.00
139_E	143_D	1.659	1.00
218_E	222_A	1.624	1.00
220_L	224_L	1.615	1.00
50_D	54_D	1.599	1.00
208_D	212_D	1.598	1.00
62_A	65_A	1.589	1.00
138_D	141_K	1.564	1.00
218_E	221_E	1.553	1.00
95_D	195_R	1.551	1.00
49_T	147_A	1.541	1.00
120_S	123_D	1.539	1.00
119_S	123_D	1.505	1.00
223_R	227_Q	1.502	1.00
108_A	180_L	1.495	1.00
50_D	143_D	1.491	1.00
60_V	137_L	1.485	1.00
48_D	51_A	1.477	1.00
58_S	62_A	1.460	1.00
57_K	137_L	1.429	1.00
233_T	237_Q	1.410	1.00
211_D	215_D	1.410	1.00
200_N	204_K	1.409	1.00
35_T	41_V	1.402	1.00
90_G	93_A	1.398	1.00
229_T	233_T	1.388	1.00
67_I	102_Q	1.362	1.00
207_D	211_D	1.357	1.00
213_L	217_L	1.356	1.00
212_D	215_D	1.355	1.00
54_D	57_K	1.332	1.00
213_L	216_R	1.321	1.00
65_A	69_T	1.300	1.00
104_Q	180_L	1.298	1.00
58_S	61_D	1.277	1.00
66_L	70_I	1.263	1.00
180_L	184_Y	1.257	1.00
69_T	72_S	1.250	1.00
49_T	143_D	1.238	1.00
222_A	225_R	1.237	1.00
209_Q	212_D	1.236	1.00
219_A	222_A	1.225	1.00
178_D	182_D	1.219	1.00
8_V	23_A	1.215	1.00
51_A	54_D	1.198	1.00
225_R	229_T	1.197	1.00
20_I	23_A	1.191	1.00
231_M	235_L	1.181	1.00
4_A	31_L	1.175	1.00
60_V	126_I	1.171	1.00
131_D	134_T	1.169	1.00
55_A	58_S	1.162	1.00
56_I	142_L	1.140	1.00
151_D	155_A	1.138	1.00
76_Y	93_A	1.133	1.00
127_T	136_S	1.131	1.00
124_I	153_V	1.126	1.00
155_A	159_G	1.120	1.00
186_D	193_T	1.113	1.00
65_A	68_S	1.103	1.00
142_L	146_L	1.103	1.00
196_T	200_N	1.099	1.00
67_I	134_T	1.097	1.00
197_D	201_S	1.096	1.00
63_Y	135_L	1.095	1.00
4_A	18_N	1.094	1.00
6_F	15_S	1.093	1.00
2_Q	5_K	1.093	1.00
70_I	102_Q	1.093	1.00
202_Q	205_D	1.086	1.00
203_I	207_D	1.080	1.00
127_T	138_D	1.079	1.00
21_T	28_T	1.063	1.00
205_D	209_Q	1.061	1.00
27_V	45_V	1.057	1.00
152_A	155_A	1.055	1.00
215_D	218_E	1.047	1.00
76_Y	83_D	1.038	1.00
140_T	144_A	1.035	1.00
71_N	102_Q	1.033	1.00
207_D	210_I	1.029	1.00
230_A	234_A	1.020	1.00
100_S	104_Q	1.017	1.00
210_I	214_E	1.012	1.00
171_T	179_D	1.010	1.00
35_T	38_G	1.009	1.00
193_T	197_D	1.008	1.00
101_L	184_Y	1.003	1.00
36_P	39_T	1.002	1.00
219_A	223_R	1.001	1.00
74_T	98_L	0.996	1.00
171_T	175_T	0.993	1.00
212_D	216_R	0.988	1.00
154_A	158_T	0.982	1.00
51_A	55_A	0.981	1.00
13_I	23_A	0.981	1.00
102_Q	106_R	0.977	1.00
151_D	154_A	0.975	1.00
156_L	173_L	0.973	1.00
56_I	126_I	0.972	1.00
234_A	237_Q	0.967	1.00
201_S	205_D	0.964	1.00
107_S	111_S	0.959	1.00
141_K	145_A	0.958	1.00
230_A	233_T	0.957	1.00
204_K	208_D	0.952	1.00
128_T	135_L	0.950	1.00
53_K	137_L	0.946	1.00
86_T	89_A	0.942	1.00
67_I	71_N	0.931	1.00
206_L	210_I	0.929	1.00
234_A	238_L	0.928	1.00
184_Y	191_L	0.927	1.00
175_T	179_D	0.926	1.00
208_D	211_D	0.919	1.00
203_I	206_L	0.916	1.00
211_D	214_E	0.916	1.00
6_F	31_L	0.915	1.00
63_Y	67_I	0.913	1.00
150_P	154_A	0.911	1.00
50_D	53_K	0.909	1.00
196_T	199_L	0.906	1.00
111_S	176_R	0.906	1.00
109_L	135_L	0.905	1.00
145_A	148_S	0.905	1.00
217_L	220_L	0.903	1.00
201_S	204_K	0.899	1.00
103_S	107_S	0.898	1.00
35_T	39_T	0.892	1.00
15_S	20_I	0.892	1.00
76_Y	87_A	0.881	1.00
23_A	29_L	0.878	1.00
52_I	55_A	0.876	1.00
74_T	102_Q	0.875	1.00
121_L	126_I	0.868	1.00
217_L	221_E	0.867	1.00
108_A	176_R	0.867	1.00
24_I	27_V	0.866	1.00
89_A	93_A	0.862	1.00
35_T	40_P	0.860	1.00
73_L	91_P	0.860	1.00
56_I	137_L	0.859	1.00
83_D	86_T	0.858	1.00
97_T	100_S	0.858	1.00
106_R	128_T	0.858	1.00
117_S	152_A	0.852	1.00
231_M	234_A	0.849	1.00
141_K	144_A	0.847	1.00
224_L	228_F	0.846	1.00
198_S	202_Q	0.844	1.00
96_S	99_R	0.844	1.00
200_N	203_I	0.839	1.00
92_L	98_L	0.833	1.00
99_R	103_S	0.830	1.00
193_T	196_T	0.829	1.00
197_D	200_N	0.828	1.00
216_R	220_L	0.824	1.00
179_D	182_D	0.821	1.00
118_Y	124_I	0.821	1.00
176_R	179_D	0.820	1.00
72_S	75_K	0.818	1.00
194_T	198_S	0.817	1.00
126_I	135_L	0.813	1.00
182_D	186_D	0.808	1.00
146_L	153_V	0.801	1.00
179_D	183_S	0.799	1.00
52_I	150_P	0.792	1.00
32_K	35_T	0.787	1.00
104_Q	183_S	0.787	1.00
204_K	207_D	0.784	1.00
62_A	66_L	0.783	1.00
60_V	135_L	0.781	1.00
205_D	208_D	0.774	1.00
180_L	183_S	0.766	1.00
195_R	198_S	0.765	1.00
20_I	29_L	0.762	1.00
6_F	12_T	0.761	1.00
99_R	102_Q	0.755	1.00
206_L	209_Q	0.753	1.00
140_T	143_D	0.749	1.00
115_S	119_S	0.748	1.00
171_T	176_R	0.748	1.00
107_S	110_S	0.747	1.00
210_I	213_L	0.745	1.00
144_A	147_A	0.743	1.00
176_R	180_L	0.741	1.00
70_I	73_L	0.738	1.00
8_V	13_I	0.736	1.00
115_S	118_Y	0.734	1.00
213_L	220_L	0.730	1.00
194_T	197_D	0.728	1.00
178_D	181_L	0.726	1.00
188_G	193_T	0.723	1.00
209_Q	213_L	0.722	1.00
104_Q	107_S	0.721	1.00
71_N	134_T	0.719	1.00
159_G	171_T	0.719	1.00
182_D	185_T	0.716	1.00
226_A	229_T	0.709	1.00
118_Y	123_D	0.707	1.00
210_I	232_E	0.706	1.00
183_S	186_D	0.703	1.00
70_I	98_L	0.702	1.00
4_A	15_S	0.695	1.00
224_L	227_Q	0.693	1.00
66_L	105_L	0.688	1.00
223_R	226_A	0.683	1.00
235_L	239_N	0.679	1.00
232_E	236_S	0.674	1.00
227_Q	230_A	0.669	1.00
108_A	111_S	0.665	1.00
49_T	52_I	0.665	1.00
118_Y	156_L	0.664	1.00
15_S	23_A	0.662	1.00
82_E	85_E	0.659	1.00
88_K	91_P	0.656	1.00
71_N	75_K	0.655	1.00
235_L	238_L	0.654	1.00
228_F	231_M	0.652	1.00
67_I	106_R	0.648	1.00
98_L	102_Q	0.646	1.00
118_Y	145_A	0.642	1.00
96_S	100_S	0.639	1.00
84_E	87_A	0.638	1.00
60_V	64_N	0.633	1.00
221_E	224_L	0.631	1.00
15_S	21_T	0.628	1.00
63_Y	105_L	0.627	1.00
159_G	175_T	0.627	1.00
49_T	150_P	0.625	1.00
175_T	178_D	0.624	1.00
57_K	60_V	0.623	1.00
118_Y	149_N	0.623	1.00
118_Y	152_A	0.620	1.00
233_T	236_S	0.620	1.00
84_E	88_K	0.619	1.00
74_T	99_R	0.617	1.00
63_Y	106_R	0.616	1.00
15_S	18_N	0.616	1.00
56_I	157_F	0.615	1.00
145_A	153_V	0.612	1.00
94_G	99_R	0.610	1.00
122_S	127_T	0.609	1.00
60_V	142_L	0.608	1.00
216_R	219_A	0.608	1.00
127_T	141_K	0.607	1.00
52_I	146_L	0.602	1.00
102_Q	108_A	0.601	1.00
157_F	169_S	0.598	1.00
195_R	199_L	0.592	1.00
27_V	30_T	0.590	1.00
97_T	191_L	0.588	1.00
41_V	44_T	0.586	1.00
34_A	37_G	0.585	1.00
172_G	176_R	0.583	1.00
73_L	76_Y	0.581	1.00
53_K	142_L	0.578	1.00
76_Y	89_A	0.576	1.00
103_S	106_R	0.574	1.00
74_T	90_G	0.574	1.00
187_S	190_G	0.574	1.00
19_T	28_T	0.572	1.00
209_Q	216_R	0.570	1.00
95_D	98_L	0.567	1.00
54_D	61_D	0.566	1.00
17_S	20_I	0.563	1.00
54_D	139_E	0.562	1.00
104_Q	108_A	0.561	1.00
75_K	93_A	0.561	1.00
160_D	175_T	0.559	1.00
160_D	170_T	0.557	1.00
47_R	150_P	0.557	1.00
159_G	169_S	0.556	1.00
228_F	232_E	0.554	1.00
33_K	39_T	0.547	1.00
46_S	49_T	0.544	1.00
22_D	31_L	0.543	1.00
102_Q	105_L	0.543	1.00
214_E	220_L	0.542	1.00
59_F	63_Y	0.542	1.00
122_S	128_T	0.541	1.00
146_L	150_P	0.540	1.00
147_A	150_P	0.535	1.00
160_D	169_S	0.532	1.00
100_S	103_S	0.532	1.00
137_L	146_L	0.529	1.00
6_F	29_L	0.526	1.00
37_G	40_P	0.522	1.00
186_D	190_G	0.519	1.00
97_T	195_R	0.516	1.00
59_F	177_L	0.516	1.00
49_T	146_L	0.516	1.00
114_G	118_Y	0.516	1.00
113_V	176_R	0.512	1.00
4_A	20_I	0.510	1.00
181_L	185_T	0.508	1.00
22_D	29_L	0.508	1.00
131_D	136_S	0.505	1.00
66_L	69_T	0.502	1.00
31_L	34_A	0.500	1.00
121_L	173_L	0.500	1.00
170_T	175_T	0.500	1.00
53_K	56_I	0.500	1.00
93_A	98_L	0.500	1.00

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3ghgA	2	0.5667	66.2	0.917	Contact Map
3swyA	3	0.1917	43.5	0.927	Contact Map
1deqA	1	0.5	33.3	0.932	Contact Map
2z5iA	3	0.2083	32.2	0.933	Contact Map
1x8yA	2	0.1583	31.3	0.933	Contact Map
1gk4A	2	0.1958	27.8	0.935	Contact Map
2xdjA	3	0.2875	27.1	0.935	Contact Map
3nftA	2	0.75	26.2	0.935	Contact Map
3tnuA	2	0.2417	22.9	0.937	Contact Map
3swfA	3	0.2167	21.9	0.938	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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