GREMLIN Database
DUF1336 - Protein of unknown function (DUF1336)
PFAM: PF07059 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 219 (208)
Sequences: 803 (520)
Seq/√Len: 36.0
META: 0.352

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
194_G209_L4.4281.00
192_D214_R4.0631.00
49_A70_V4.0371.00
15_N19_D3.9781.00
46_D74_Q3.6351.00
13_G23_V3.2351.00
69_F164_I3.2241.00
47_H161_Y3.0811.00
12_R16_Y2.9991.00
168_I175_A2.9641.00
199_G205_L2.7381.00
13_G19_D2.7001.00
124_I196_L2.6281.00
71_V195_F2.5411.00
74_Q81_S2.4581.00
120_R204_E2.3671.00
31_Y65_L2.3240.99
49_A165_D2.1920.99
35_G85_Y2.1660.99
11_V124_I2.1230.99
44_K74_Q2.0280.99
37_D85_Y2.0170.98
11_V23_V1.9310.98
47_H165_D1.9180.98
116_F120_R1.9110.98
29_A209_L1.8780.98
34_V68_I1.8450.97
13_G16_Y1.7340.96
12_R22_K1.7010.96
162_L197_I1.7010.96
73_L168_I1.6930.95
36_V213_V1.6830.95
194_G212_C1.6640.95
33_L86_F1.6410.95
9_F194_G1.5920.94
14_P202_E1.5680.93
36_V84_L1.5450.93
16_Y198_E1.5220.92
22_K122_K1.5170.92
145_L166_V1.4860.91
168_I179_L1.4740.91
114_D117_R1.4370.90
88_A96_D1.4360.90
108_R204_E1.4320.89
29_A78_P1.4160.89
59_S71_V1.4150.89
85_Y185_Y1.3980.88
38_W82_L1.3670.87
11_V209_L1.3600.87
136_K139_G1.3600.87
92_E95_P1.3250.85
117_R153_Y1.3240.85
17_L199_G1.3170.85
139_G145_L1.3120.85
59_S63_G1.3090.85
16_Y22_K1.2910.84
142_P148_A1.2770.83
156_G160_N1.2760.83
106_L109_F1.2710.83
12_R122_K1.2660.83
42_D128_V1.2640.83
3_E32_K1.2450.82
146_G153_Y1.2390.81
48_I165_D1.2330.81
146_G171_S1.2160.80
118_N153_Y1.2100.80
20_K141_K1.2060.79
127_I130_G1.1880.78
6_G210_L1.1860.78
48_I162_L1.1760.78
131_P134_V1.1710.77
171_S178_I1.1620.77
27_G38_W1.1510.76
127_I191_V1.1440.76
122_K142_P1.1360.75
31_Y171_S1.1190.74
66_P76_P1.1120.74
12_R142_P1.1000.73
215_L219_D1.1000.73
72_N127_I1.0980.73
143_V148_A1.0950.72
77_G169_G1.0840.72
75_V126_R1.0840.72
45_I74_Q1.0810.71
10_K24_P1.0780.71
18_K50_R1.0750.71
32_K166_V1.0720.71
111_N155_R1.0600.70
154_F161_Y1.0570.70
72_N81_S1.0560.70
69_F162_L1.0510.69
86_F211_G1.0470.69
130_G134_V1.0360.68
35_G72_N1.0330.68
129_N132_W1.0330.68
74_Q165_D1.0290.68
37_D72_N1.0240.67
177_K198_E1.0190.67
121_F195_F1.0050.66
47_H154_F0.9970.65
134_V185_Y0.9950.65
34_V53_E0.9870.64
132_W139_G0.9750.63
190_V214_R0.9720.63
149_L153_Y0.9670.63
89_T95_P0.9590.62
134_V181_L0.9570.62
120_R160_N0.9550.62
86_F185_Y0.9550.62
130_G139_G0.9540.62
77_G161_Y0.9480.61
15_N23_V0.9470.61
73_L137_A0.9290.60
138_V145_L0.9250.59
74_Q169_G0.9240.59
198_E207_E0.9200.59
36_V215_L0.9190.59
111_N121_F0.9160.59
127_I134_V0.9130.58
127_I138_V0.9110.58
81_S165_D0.9110.58
167_D192_D0.9040.58
52_P85_Y0.9000.57
5_D70_V0.8990.57
80_Y183_L0.8980.57
30_L37_D0.8980.57
48_I83_V0.8960.57
35_G182_V0.8910.56
47_H50_R0.8830.56
68_I87_K0.8830.56
18_K62_S0.8820.56
14_P36_V0.8810.56
22_K142_P0.8700.55
124_I129_N0.8700.55
118_N161_Y0.8680.54
175_A178_I0.8660.54
144_L200_Q0.8630.54
18_K202_E0.8610.54
39_F45_I0.8570.53
39_F120_R0.8570.53
17_L78_P0.8470.53
52_P199_G0.8450.52
81_S168_I0.8440.52
129_N134_V0.8410.52
72_N167_D0.8400.52
45_I68_I0.8370.52
41_S90_E0.8360.52
11_V173_V0.8340.51
106_L110_V0.8310.51
2_S210_L0.8290.51
1_W11_V0.8200.50
108_R112_G0.8190.50
189_L217_K0.7950.48
30_L180_G0.7930.48
130_G135_K0.7910.48
189_L192_D0.7820.47
33_L170_S0.7790.47
146_G169_G0.7790.47
50_R164_I0.7750.46
69_F123_L0.7740.46
13_G172_S0.7720.46
38_W148_A0.7640.45
130_G138_V0.7590.45
161_Y165_D0.7590.45
13_G210_L0.7580.45
125_P146_G0.7580.45
16_Y122_K0.7580.45
117_R171_S0.7530.44
6_G209_L0.7460.44
59_S62_S0.7410.43
156_G162_L0.7390.43
71_V86_F0.7330.43
127_I189_L0.7330.43
37_D176_R0.7260.42
28_D142_P0.7180.41
4_P192_D0.7170.41
63_G68_I0.7120.41
49_A144_L0.7070.41
135_K138_V0.7060.40
105_A109_F0.7040.40
186_L191_V0.7010.40
128_V192_D0.6990.40
131_P135_K0.6970.40
156_G161_Y0.6910.39
150_Q153_Y0.6880.39
33_L36_V0.6870.39
134_V171_S0.6850.39
26_A97_G0.6820.38
46_D75_V0.6810.38
77_G80_Y0.6800.38
30_L84_L0.6800.38
213_V219_D0.6790.38
125_P180_G0.6780.38
176_R187_K0.6760.38
105_A166_V0.6760.38
20_K27_G0.6760.38
9_F212_C0.6760.38
84_L193_L0.6750.38
39_F73_L0.6710.38
90_E94_L0.6670.37
15_N18_K0.6660.37
138_V175_A0.6660.37
169_G173_V0.6640.37
22_K198_E0.6630.37
183_L193_L0.6560.36
201_T211_G0.6550.36
173_V176_R0.6550.36
39_F164_I0.6550.36
135_K170_S0.6530.36
169_G176_R0.6520.36
123_L178_I0.6490.36
90_E95_P0.6490.36
79_G171_S0.6480.36
83_V123_L0.6480.36
35_G73_L0.6470.36
156_G217_K0.6460.36
73_L127_I0.6440.35
44_K192_D0.6440.35
16_Y43_K0.6380.35
129_N171_S0.6360.35
131_P136_K0.6340.35
78_P179_L0.6340.35
161_Y203_E0.6300.34
36_V82_L0.6300.34
141_K191_V0.6280.34
166_V171_S0.6260.34
120_R200_Q0.6230.34
139_G170_S0.6220.34
121_F144_L0.6220.34
107_K112_G0.6210.34
73_L216_N0.6210.34
11_V196_L0.6180.33
143_V166_V0.6170.33
55_L75_V0.6170.33
94_L97_G0.6170.33
125_P193_L0.6150.33
67_F146_G0.6090.33
142_P207_E0.6090.33
50_R193_L0.6050.32
35_G95_P0.6040.32
19_D182_V0.6010.32
145_L151_C0.6000.32
190_V217_K0.5990.32
75_V179_L0.5990.32
109_F117_R0.5960.32
28_D61_A0.5940.32
90_E96_D0.5890.31
96_D108_R0.5890.31
45_I81_S0.5880.31
84_L213_V0.5860.31
20_K50_R0.5850.31
24_P86_F0.5850.31
76_P195_F0.5840.31
1_W12_R0.5830.31
137_A178_I0.5770.30
59_S203_E0.5770.30
79_G169_G0.5760.30
49_A92_E0.5750.30
19_D205_L0.5730.30
30_L155_R0.5710.30
14_P205_L0.5710.30
98_S168_I0.5700.30
19_D202_E0.5670.30
120_R165_D0.5660.29
17_L144_L0.5600.29
149_L169_G0.5600.29
23_V179_L0.5590.29
33_L213_V0.5590.29
166_V189_L0.5580.29
19_D121_F0.5560.29
127_I173_V0.5540.29
126_R145_L0.5530.29
89_T97_G0.5520.28
128_V167_D0.5510.28
197_I209_L0.5460.28
122_K207_E0.5450.28
84_L219_D0.5430.28
109_F167_D0.5410.28
134_V137_A0.5340.27
125_P130_G0.5340.27
3_E29_A0.5310.27
142_P187_K0.5310.27
117_R139_G0.5310.27
127_I179_L0.5300.27
31_Y66_P0.5300.27
75_V209_L0.5290.27
135_K139_G0.5290.27
45_I197_I0.5290.27
116_F197_I0.5270.27
9_F139_G0.5260.27
145_L176_R0.5240.27
168_I183_L0.5170.26
86_F145_L0.5170.26
131_P190_V0.5160.26
30_L121_F0.5160.26
97_G188_S0.5150.26
107_K199_G0.5140.26
61_A64_K0.5120.26
47_H156_G0.5120.26
124_I194_G0.5120.26
94_L107_K0.5100.26
196_L199_G0.5090.26
135_K180_G0.5090.26
46_D154_F0.5090.26
137_A162_L0.5060.25
85_Y162_L0.5060.25
164_I213_V0.5020.25
44_K119_S0.5010.25
167_D214_R0.5010.25
184_G188_S0.5010.25
160_N169_G0.5000.25
117_R130_G0.5000.25
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4dayC 1 0.0548 21.9 0.954 Contact Map
2jvfA 1 0.3379 2.8 0.97 Contact Map
1r8uA 1 0.1735 2.6 0.97 Contact Map
3epzA 1 0.2329 2.4 0.971 Contact Map
4pgiA 1 0.2055 2.3 0.971 Contact Map
3if8B 1 0.5205 2 0.972 Contact Map
2b9sB 1 0.0639 2 0.972 Contact Map
1p4qA 1 0.2283 2 0.972 Contact Map
2iiiA 2 0.5479 2 0.972 Contact Map
2gj2A 3 0.137 1.9 0.972 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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