GREMLIN Database
HET - Heterokaryon incompatibility protein (HET)
PFAM: PF06985 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 146 (144)
Sequences: 9810 (9169)
Seq/√Len: 764.1
META: 0.428

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
60_K64_V3.8241.00
2_E76_L3.3711.00
79_V137_W3.0921.00
53_N56_D3.0451.00
74_A77_V2.9451.00
31_Q35_R2.7281.00
2_E43_R2.7151.00
3_A77_V2.5301.00
30_L47_I2.4891.00
57_D61_S2.3941.00
66_L69_D2.3871.00
137_W143_V2.3471.00
2_E42_I2.2981.00
63_Q66_L2.2051.00
4_L33_A2.2021.00
7_V136_P2.1821.00
57_D60_K2.1651.00
3_A74_A2.1591.00
53_N59_E2.0551.00
42_I75_Y2.0261.00
6_Y49_A2.0131.00
15_E31_Q1.9721.00
28_K32_D1.8651.00
32_D83_A1.7851.00
6_Y30_L1.7681.00
33_A37_L1.7341.00
69_D73_N1.6561.00
85_D88_S1.6411.00
16_T25_S1.5001.00
1_Y73_N1.4371.00
2_E78_I1.4271.00
2_E37_L1.4121.00
15_E28_K1.3901.00
136_P139_T1.3821.00
24_L50_L1.3781.00
68_G72_S1.3521.00
84_E88_S1.3211.00
58_E61_S1.3171.00
132_L135_R1.2871.00
36_V83_A1.2641.00
30_L33_A1.2391.00
19_V24_L1.2361.00
67_M71_Y1.2321.00
83_A88_S1.2041.00
36_V90_L1.1741.00
4_L47_I1.1681.00
61_S64_V1.1651.00
17_I21_G1.1431.00
135_R138_F1.1401.00
59_E63_Q1.1271.00
77_V143_V1.1261.00
28_K31_Q1.1091.00
128_A132_L1.1081.00
62_H66_L1.1021.00
32_D36_V1.0931.00
3_A70_I1.0911.00
79_V143_V1.0891.00
2_E75_Y1.0851.00
23_P31_Q1.0831.00
132_L138_F1.0731.00
7_V137_W1.0721.00
63_Q70_I1.0641.00
17_I20_N1.0461.00
16_T20_N1.0421.00
16_T23_P1.0411.00
33_A45_L1.0391.00
6_Y86_S1.0391.00
61_S65_A1.0371.00
8_W54_Q1.0171.00
36_V80_W1.0151.00
13_N21_G0.9961.00
14_T17_I0.9791.00
7_V143_V0.9661.00
81_L138_F0.9581.00
35_R39_R0.9461.00
84_E90_L0.9281.00
141_A144_L0.9261.00
60_K65_A0.9151.00
26_L31_Q0.9121.00
56_D59_E0.9091.00
30_L80_W0.9071.00
29_N89_G0.9021.00
52_I63_Q0.9021.00
119_P122_L0.9011.00
36_V78_I0.8991.00
13_N28_K0.8981.00
37_L42_I0.8961.00
38_R43_R0.8921.00
6_Y29_N0.8881.00
26_L34_L0.8851.00
52_I60_K0.8841.00
40_L43_R0.8751.00
66_L70_I0.8701.00
37_L45_L0.8661.00
81_L135_R0.8641.00
54_Q60_K0.8541.00
90_L94_R0.8491.00
18_L23_P0.8441.00
27_P30_L0.8401.00
82_G135_R0.8371.00
81_L137_W0.8281.00
88_S135_R0.8201.00
71_Y145_Q0.8001.00
81_L132_L0.7911.00
4_L30_L0.7901.00
138_F144_L0.7861.00
67_M140_R0.7801.00
13_N16_T0.7731.00
27_P49_A0.7651.00
2_E45_L0.7571.00
4_L80_W0.7531.00
14_T25_S0.7451.00
91_L125_L0.7421.00
58_E62_H0.7361.00
42_I76_L0.7351.00
37_L78_I0.7341.00
138_F143_V0.7331.00
102_L105_E0.7291.00
29_N49_A0.7211.00
101_P104_L0.7211.00
59_E62_H0.7181.00
16_T19_V0.7171.00
25_S28_K0.7151.00
94_R97_R0.7141.00
69_D72_S0.7121.00
108_P111_L0.7091.00
8_W27_P0.7061.00
118_L122_L0.7051.00
19_V138_F0.7041.00
119_P123_S0.7031.00
129_L133_L0.7021.00
109_D113_L0.7021.00
4_L37_L0.7011.00
84_E135_R0.6951.00
18_L21_G0.6931.00
76_L79_V0.6931.00
26_L50_L0.6881.00
92_A95_L0.6861.00
90_L93_L0.6831.00
15_E18_L0.6821.00
13_N27_P0.6761.00
62_H65_A0.6691.00
91_L128_A0.6681.00
122_L125_L0.6671.00
39_R43_R0.6641.00
80_W83_A0.6601.00
27_P86_S0.6601.00
45_L78_I0.6571.00
10_D13_N0.6571.00
106_L109_D0.6571.00
30_L50_L0.6561.00
95_L98_R0.6551.00
100_A104_L0.6541.00
11_P27_P0.6531.00
14_T27_P0.6521.00
113_L117_L0.6371.00
29_N33_A0.6361.00
26_L29_N0.6331.00
123_S126_W0.6321.00
1_Y70_I0.6231.00
107_L111_L0.6221.00
50_L53_N0.6151.00
130_R134_S0.6151.00
7_V60_K0.6121.00
36_V40_L0.6121.00
40_L76_L0.6111.00
7_V141_A0.6111.00
13_N18_L0.6101.00
107_L110_S0.6051.00
14_T18_L0.6021.00
7_V29_N0.6011.00
26_L30_L0.5961.00
33_A47_I0.5961.00
41_G44_Y0.5941.00
29_N80_W0.5911.00
8_W67_M0.5891.00
71_Y77_V0.5851.00
22_P25_S0.5851.00
6_Y47_I0.5841.00
33_A80_W0.5811.00
96_L129_L0.5791.00
91_L95_L0.5761.00
56_D61_S0.5701.00
13_N20_N0.5701.00
32_D80_W0.5691.00
70_I77_V0.5681.00
121_L125_L0.5651.00
30_L49_A0.5651.00
29_N32_D0.5641.00
32_D35_R0.5631.00
109_D114_D0.5611.00
40_L44_Y0.5541.00
17_I50_L0.5491.00
7_V10_D0.5441.00
48_D54_Q0.5421.00
112_R118_L0.5421.00
34_L37_L0.5421.00
100_A103_S0.5401.00
43_R78_I0.5341.00
24_L35_R0.5331.00
120_P126_W0.5321.00
91_L94_R0.5311.00
85_D131_D0.5291.00
15_E19_V0.5251.00
10_D14_T0.5241.00
108_P112_R0.5241.00
95_L99_L0.5231.00
109_D112_R0.5211.00
93_L97_R0.5181.00
120_P128_A0.5171.00
99_L129_L0.5131.00
2_E39_R0.5091.00
142_W146_E0.5031.00
124_D128_A0.5021.00
64_V68_G0.5001.00
49_A63_Q0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1ltrD 5 0.5 3.5 0.934 Contact Map
4x9cA 3 0.3014 2.2 0.941 Contact Map
2jexA 1 0.6438 2 0.943 Contact Map
2nnuA 1 0.6644 1.7 0.945 Contact Map
2kigA 1 0.3904 1.5 0.946 Contact Map
1tueB 1 0.6918 1.4 0.947 Contact Map
4m1uB 3 0.2534 1.4 0.947 Contact Map
3kk4A 2 0.5548 1.3 0.948 Contact Map
4b9jA 1 0.2671 1.3 0.949 Contact Map
4n7bA 1 0.8904 1.2 0.95 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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