GREMLIN Database
DUF1218 - Protein of unknown function (DUF1218)
PFAM: PF06749 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 91 (90)
Sequences: 693 (435)
Seq/√Len: 45.8
META: 0.029

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
56_G79_F2.8781.00
59_R74_L2.8301.00
24_R28_S2.7981.00
22_G25_L2.4921.00
16_G19_F2.3461.00
45_T89_T2.2301.00
52_L85_L2.1881.00
2_A6_L1.9700.99
84_V88_V1.9360.99
47_A51_A1.8620.99
80_A84_V1.8530.99
23_K27_P1.7460.98
9_Q43_W1.6750.98
22_G27_P1.6220.97
53_L82_G1.6020.97
82_G90_A1.5970.97
66_Y69_Y1.4530.95
17_G20_C1.4460.95
14_V51_A1.4430.94
9_Q20_C1.4350.94
10_V15_A1.4210.94
21_C27_P1.4200.94
10_V36_V1.3550.92
5_L83_A1.2990.91
45_T52_L1.2830.90
47_A85_L1.2780.90
25_L30_A1.2650.90
63_H77_G1.2640.89
27_P48_I1.2540.89
79_F82_G1.2380.88
4_L23_K1.2350.88
73_T80_A1.2290.88
80_A86_S1.2200.88
34_L37_V1.1810.86
3_L89_T1.1800.86
46_F89_T1.1730.86
31_N90_A1.1720.86
23_K30_A1.1670.85
53_L57_A1.1600.85
37_V53_L1.1590.85
50_E61_A1.1530.85
37_V41_L1.1460.84
22_G30_A1.1290.83
40_V47_A1.0880.81
38_F80_A1.0820.81
35_A43_W1.0520.79
69_Y76_K1.0170.76
44_I47_A1.0120.76
21_C42_S1.0000.75
18_C57_A0.9910.74
77_G87_L0.9840.74
3_L50_E0.9770.73
26_S29_G0.9750.73
13_N39_F0.9630.72
25_L28_S0.9610.72
26_S47_A0.9480.71
78_V85_L0.9390.70
26_S30_A0.9180.69
26_S37_V0.9090.68
12_A15_A0.9030.67
73_T76_K0.8880.66
16_G20_C0.8870.66
62_Y66_Y0.8810.65
58_A76_K0.8800.65
57_A60_N0.8650.64
11_I90_A0.8540.63
12_A16_G0.8500.63
31_N35_A0.8460.62
32_R38_F0.8320.61
24_R27_P0.8270.60
52_L77_G0.8210.60
40_V43_W0.8190.60
10_V75_R0.8140.59
77_G81_A0.8110.59
42_S47_A0.8080.59
26_S78_V0.7950.57
18_C82_G0.7940.57
75_R78_V0.7930.57
2_A50_E0.7880.57
26_S41_L0.7840.56
66_Y70_S0.7800.56
21_C25_L0.7770.56
64_T80_A0.7540.53
11_I70_S0.7490.53
41_L47_A0.7490.53
55_A78_V0.7370.52
12_A21_C0.7320.51
45_T75_R0.7250.51
34_L38_F0.7250.51
1_A4_L0.7210.50
38_F51_A0.7050.49
1_A41_L0.7010.48
59_R63_H0.6640.45
1_A66_Y0.6610.44
56_G61_A0.6480.43
13_N87_L0.6470.43
35_A40_V0.6470.43
12_A20_C0.6390.42
75_R83_A0.6370.42
11_I21_C0.6360.42
3_L25_L0.6330.42
62_Y67_R0.6280.41
52_L62_Y0.6260.41
33_T41_L0.6220.41
9_Q13_N0.6220.41
42_S54_L0.6210.40
49_A86_S0.6200.40
37_V57_A0.6130.40
34_L87_L0.6090.39
33_T44_I0.5980.38
14_V26_S0.5890.37
5_L70_S0.5870.37
72_Y85_L0.5770.36
20_C35_A0.5730.36
62_Y82_G0.5710.36
54_L61_A0.5680.36
85_L88_V0.5680.36
44_I67_R0.5640.35
32_R46_F0.5640.35
72_Y76_K0.5610.35
28_S37_V0.5600.35
16_G90_A0.5570.35
20_C27_P0.5370.33
12_A56_G0.5340.33
55_A63_H0.5330.32
33_T37_V0.5310.32
5_L86_S0.5300.32
21_C30_A0.5290.32
33_T48_I0.5270.32
13_N17_G0.5270.32
18_C35_A0.5200.31
39_F81_A0.5170.31
33_T77_G0.5060.30
18_C60_N0.5040.30
55_A73_T0.5000.30
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3x29A 1 0.9231 41.2 0.852 Contact Map
4p79A 1 0.989 31.4 0.862 Contact Map
4he8J 1 0.2088 3.5 0.911 Contact Map
3pblA 1 0.9451 2.7 0.916 Contact Map
3lb6C 1 0 1.8 0.923 Contact Map
1v54K 1 0.2747 1.5 0.927 Contact Map
4mqsA 1 0.967 1.5 0.927 Contact Map
1cd1A 1 0 1.4 0.928 Contact Map
2ivdA 1 0.1868 1.4 0.929 Contact Map
3uonA 1 0.967 1.3 0.929 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0051 seconds.