GREMLIN Database
PIG-U - GPI transamidase subunit PIG-U
PFAM: PF06728 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 383 (366)
Sequences: 700 (509)
Seq/√Len: 26.6
META: 0.091

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
29_T320_T4.1181.00
351_L355_S3.6421.00
312_F317_S3.3331.00
283_Q286_P3.1671.00
318_Y323_D3.0331.00
285_H289_Y2.9431.00
316_K323_D2.8521.00
221_F225_L2.7931.00
152_L174_L2.7481.00
4_V92_L2.7160.99
358_G362_W2.5730.99
355_S359_P2.5660.99
318_Y322_G2.4400.99
281_V378_A2.3950.99
68_P74_P2.2890.98
349_L353_Y2.2650.98
317_S322_G2.2490.98
258_P261_G2.2320.98
228_L232_S2.2200.98
156_A168_A2.2190.98
159_L171_A2.1830.97
255_D259_N2.0640.96
199_Q204_S1.9950.96
242_F247_Y1.9900.96
265_Y269_E1.9680.95
27_V30_P1.9640.95
14_F18_P1.9620.95
146_S322_G1.9410.95
322_G326_L1.9330.95
313_V318_Y1.9270.95
94_T154_L1.9260.95
362_W366_I1.9120.95
240_W244_E1.9110.95
269_E274_F1.8970.94
264_W269_E1.8630.94
40_G50_P1.8540.94
25_V31_L1.8280.93
181_Y250_H1.7910.92
59_T177_H1.7820.92
201_L204_S1.7600.92
53_G57_H1.7550.92
7_R10_L1.7470.91
280_G283_Q1.7410.91
33_S36_R1.7110.90
41_A45_T1.6970.90
350_L353_Y1.6840.90
352_L356_L1.6810.90
126_V331_L1.6710.89
313_V316_K1.6660.89
320_T323_D1.6590.89
66_L145_T1.6570.89
110_S183_I1.6510.89
267_F271_F1.6460.89
177_H379_I1.6360.88
56_Y66_L1.6350.88
42_F46_H1.6320.88
310_G327_Y1.6190.88
267_F278_F1.6180.88
32_T146_S1.6100.87
316_K326_L1.5810.87
323_D326_L1.5680.86
267_F270_M1.5340.85
320_T326_L1.5270.85
40_G66_L1.5220.84
363_H370_S1.5150.84
206_F209_S1.5120.84
339_P344_S1.5110.84
101_I154_L1.5090.84
200_S203_K1.5060.84
29_T32_T1.4980.83
277_F378_A1.4950.83
44_Y373_A1.4910.83
95_A161_V1.4860.83
325_G329_S1.4760.82
217_L221_F1.4750.82
180_L191_L1.4660.82
319_P323_D1.4610.82
351_L358_G1.4580.82
143_G378_A1.4530.81
44_Y64_W1.4520.81
233_Y237_G1.4510.81
225_L230_L1.4510.81
36_R255_D1.4390.81
40_G43_L1.4330.80
133_N328_L1.4310.80
365_W374_N1.4230.80
320_T324_F1.4210.80
367_Y370_S1.4160.80
90_A94_T1.4090.79
30_P33_S1.3930.79
316_K322_G1.3890.78
363_H368_A1.3870.78
199_Q202_L1.3820.78
365_W372_N1.3780.78
336_H339_P1.3780.78
164_N170_F1.3770.78
225_L229_L1.3750.78
185_L309_T1.3710.77
274_F278_F1.3670.77
177_H300_D1.3660.77
318_Y326_L1.3610.77
212_R223_L1.3520.76
277_F281_V1.3510.76
57_H249_Y1.3500.76
309_T312_F1.3380.76
208_S211_L1.3360.75
294_S308_L1.3340.75
169_A246_T1.3330.75
25_V35_K1.3320.75
50_P56_Y1.3270.75
344_S348_L1.3180.74
359_P362_W1.3170.74
232_S238_G1.3170.74
233_Y242_F1.3160.74
198_R202_L1.3110.74
345_F367_Y1.3100.74
331_L352_L1.3050.74
199_Q203_K1.3040.74
159_L167_L1.3020.74
217_L225_L1.2950.73
244_E252_T1.2920.73
376_F380_T1.2910.73
135_L355_S1.2790.72
59_T327_Y1.2770.72
94_T151_L1.2690.72
313_V317_S1.2680.72
33_S53_G1.2630.71
202_L212_R1.2530.71
229_L242_F1.2510.70
90_A330_L1.2500.70
350_L354_A1.2500.70
225_L232_S1.2500.70
93_L101_I1.2450.70
251_L328_L1.2400.70
157_L199_Q1.2380.70
27_V31_L1.2360.70
176_V221_F1.2330.69
250_H317_S1.2310.69
52_D241_E1.2310.69
216_K220_L1.2290.69
98_L138_L1.2290.69
213_F216_K1.2210.69
40_G63_L1.2190.68
221_F227_A1.2130.68
137_I140_C1.2100.68
282_F286_P1.2040.67
91_D95_A1.2040.67
269_E277_F1.2010.67
90_A251_L1.1870.66
317_S323_D1.1870.66
324_F383_F1.1730.65
201_L206_F1.1720.65
217_L220_L1.1710.65
182_P250_H1.1640.65
173_A239_G1.1600.65
256_L278_F1.1480.64
69_L74_P1.1430.63
218_L224_S1.1410.63
272_D275_R1.1400.63
234_L238_G1.1330.63
222_L225_L1.1240.62
249_Y377_Y1.1210.62
359_P363_H1.1180.62
157_L209_S1.1170.62
27_V142_R1.1160.61
264_W270_M1.1130.61
214_F217_L1.1130.61
43_L46_H1.1130.61
152_L155_L1.1060.61
184_I287_L1.1030.61
355_S364_L1.1030.61
208_S222_L1.0970.60
127_A131_L1.0920.60
148_F155_L1.0870.59
267_F274_F1.0810.59
40_G150_N1.0800.59
271_F278_F1.0790.59
18_P154_L1.0730.58
220_L224_S1.0670.58
266_F278_F1.0670.58
39_E53_G1.0630.58
198_R204_S1.0570.57
49_S241_E1.0540.57
372_N375_F1.0530.57
172_L176_V1.0450.56
58_Y371_G1.0450.56
151_L226_L1.0300.55
90_A151_L1.0240.55
79_S82_F1.0230.55
351_L362_W1.0210.55
303_F306_F1.0190.54
358_G366_I1.0170.54
274_F277_F1.0160.54
318_Y324_F1.0160.54
160_A192_L1.0160.54
354_A358_G1.0150.54
375_F380_T1.0140.54
185_L317_S1.0140.54
148_F352_L1.0090.54
182_P259_N1.0070.54
340_Y344_S1.0050.53
84_K143_G1.0050.53
344_S347_L1.0030.53
248_G364_L1.0030.53
62_L178_L0.9960.53
368_A383_F0.9960.53
339_P351_L0.9920.52
211_L231_L0.9910.52
228_L233_Y0.9900.52
316_K319_P0.9900.52
30_P36_R0.9890.52
184_I308_L0.9880.52
266_F271_F0.9840.52
6_L10_L0.9800.52
49_S87_F0.9790.51
149_T179_S0.9660.50
152_L178_L0.9660.50
269_E279_L0.9610.50
127_A183_I0.9600.50
219_L227_A0.9570.50
22_S27_V0.9550.50
9_L14_F0.9550.50
3_A131_L0.9540.50
29_T33_S0.9510.49
267_F282_F0.9510.49
175_A299_R0.9490.49
278_F293_L0.9490.49
26_E30_P0.9440.49
15_P18_P0.9380.48
65_L82_F0.9380.48
271_F292_P0.9380.48
293_L311_I0.9340.48
224_S228_L0.9340.48
347_L355_S0.9330.48
87_F148_F0.9320.48
219_L223_L0.9230.47
51_Y246_T0.9190.47
209_S215_L0.9170.47
4_V16_S0.9170.47
316_K320_T0.9150.47
349_L356_L0.9140.47
310_G314_I0.9130.47
318_Y321_S0.9120.46
153_V183_I0.9120.46
278_F315_F0.9080.46
266_F269_E0.9070.46
201_L205_R0.9040.46
5_A9_L0.9020.46
96_L168_A0.8990.45
221_F226_L0.8990.45
338_L343_Y0.8910.45
37_L40_G0.8890.45
248_G316_K0.8860.45
313_V322_G0.8850.44
66_L331_L0.8840.44
211_L221_F0.8820.44
5_A13_L0.8790.44
222_L243_L0.8770.44
10_L27_V0.8720.43
225_L228_L0.8710.43
22_S30_P0.8690.43
258_P264_W0.8690.43
3_A7_R0.8590.43
209_S213_F0.8570.42
65_L164_N0.8560.42
348_L355_S0.8560.42
103_R106_Q0.8540.42
218_L223_L0.8530.42
226_L230_L0.8520.42
210_L219_L0.8510.42
379_I382_V0.8440.41
26_E29_T0.8410.41
151_L174_L0.8370.41
277_F379_I0.8370.41
85_L214_F0.8360.41
261_G264_W0.8340.41
218_L221_F0.8340.41
313_V323_D0.8330.41
144_S257_T0.8320.41
220_L238_G0.8320.41
220_L229_L0.8300.40
355_S363_H0.8290.40
316_K324_F0.8270.40
10_L16_S0.8260.40
166_L293_L0.8250.40
177_H232_S0.8240.40
202_L216_K0.8240.40
369_G378_A0.8210.40
15_P23_S0.8200.40
122_S296_R0.8150.39
273_H277_F0.8150.39
340_Y351_L0.8140.39
229_L232_S0.8130.39
123_A127_A0.8130.39
104_S107_K0.8080.39
201_L207_S0.8070.39
351_L366_I0.8070.39
338_L351_L0.8060.39
130_Y150_N0.8060.39
341_M344_S0.7980.38
258_P292_P0.7950.38
49_S143_G0.7940.38
68_P75_N0.7930.38
275_R278_F0.7900.38
138_L346_V0.7860.37
8_L13_L0.7840.37
9_L111_R0.7840.37
51_Y60_P0.7820.37
270_M274_F0.7820.37
270_M278_F0.7810.37
214_F226_L0.7810.37
269_E278_F0.7810.37
14_F17_L0.7760.37
35_K148_F0.7740.36
154_L187_L0.7730.36
249_Y255_D0.7720.36
169_A186_L0.7710.36
39_E43_L0.7710.36
21_L27_V0.7700.36
266_F270_M0.7700.36
41_A48_V0.7680.36
145_T269_E0.7640.36
296_R350_L0.7590.35
77_Y124_L0.7560.35
157_L305_A0.7560.35
274_F282_F0.7560.35
363_H380_T0.7550.35
152_L156_A0.7550.35
92_L203_K0.7540.35
261_G271_F0.7520.35
217_L226_L0.7510.35
287_L291_L0.7510.35
198_R203_K0.7510.35
6_L86_L0.7510.35
142_R253_R0.7500.35
92_L191_L0.7500.35
149_T178_L0.7460.35
357_L361_F0.7450.34
359_P375_F0.7430.34
29_T36_R0.7380.34
131_L279_L0.7380.34
227_A295_I0.7350.34
179_S316_K0.7350.34
309_T317_S0.7320.34
192_L195_D0.7230.33
47_G50_P0.7190.33
185_L301_P0.7170.33
9_L15_P0.7170.33
39_E58_Y0.7160.33
3_A8_L0.7130.32
86_L219_L0.7090.32
230_L295_I0.7070.32
168_A243_L0.7050.32
180_L332_P0.7050.32
113_F120_K0.7040.32
228_L238_G0.7030.32
346_V357_L0.7030.32
52_D338_L0.7010.32
332_P362_W0.6990.31
273_H362_W0.6990.31
198_R205_R0.6950.31
13_L18_P0.6930.31
216_K225_L0.6910.31
198_R201_L0.6900.31
277_F342_R0.6880.31
218_L225_L0.6880.31
294_S311_I0.6870.31
139_I324_F0.6870.31
216_K227_A0.6850.31
39_E142_R0.6830.30
141_V256_L0.6820.30
339_P355_S0.6770.30
345_F350_L0.6760.30
350_L361_F0.6720.30
183_I230_L0.6720.30
322_G325_G0.6720.30
92_L206_F0.6710.30
142_R378_A0.6700.30
94_T152_L0.6690.30
361_F379_I0.6690.30
368_A380_T0.6660.29
220_L228_L0.6650.29
100_R207_S0.6650.29
41_A66_L0.6640.29
358_G361_F0.6640.29
281_V284_L0.6640.29
271_F285_H0.6630.29
229_L299_R0.6620.29
139_I142_R0.6610.29
278_F292_P0.6610.29
39_E323_D0.6600.29
271_F277_F0.6600.29
277_F288_I0.6600.29
31_L36_R0.6590.29
371_G382_V0.6560.29
287_L292_P0.6540.29
3_A14_F0.6530.29
45_T225_L0.6530.29
224_S229_L0.6520.28
180_L293_L0.6510.28
93_L190_L0.6510.28
271_F274_F0.6510.28
285_H377_Y0.6500.28
264_W267_F0.6480.28
171_A174_L0.6470.28
261_G269_E0.6460.28
243_L320_T0.6420.28
269_E272_D0.6410.28
98_L126_V0.6400.28
223_L226_L0.6400.28
22_S31_L0.6390.28
334_F371_G0.6370.28
13_L17_L0.6370.28
207_S234_L0.6360.28
5_A305_A0.6360.28
312_F318_Y0.6350.27
233_Y240_W0.6340.27
353_Y361_F0.6340.27
128_A295_I0.6340.27
319_P322_G0.6330.27
229_L236_L0.6320.27
210_L214_F0.6310.27
256_L275_R0.6310.27
353_Y378_A0.6280.27
49_S56_Y0.6270.27
208_S216_K0.6250.27
164_N360_V0.6250.27
105_L300_D0.6250.27
148_F193_L0.6240.27
83_G181_Y0.6220.27
55_T58_Y0.6210.27
63_L373_A0.6190.27
264_W274_F0.6170.26
145_T264_W0.6170.26
233_Y238_G0.6170.26
85_L213_F0.6150.26
220_L225_L0.6150.26
142_R320_T0.6140.26
95_A218_L0.6120.26
162_K202_L0.6120.26
152_L234_L0.6110.26
129_L162_K0.6110.26
89_L332_P0.6090.26
175_A182_P0.6070.26
171_A175_A0.6040.26
79_S230_L0.6030.26
343_Y355_S0.6030.26
241_E297_L0.6000.25
9_L13_L0.6000.25
189_L376_F0.5980.25
153_V178_L0.5970.25
205_R352_L0.5950.25
251_L281_V0.5940.25
220_L226_L0.5940.25
242_F367_Y0.5910.25
261_G323_D0.5910.25
205_R211_L0.5890.25
227_A346_V0.5880.25
258_P274_F0.5880.25
147_S335_P0.5870.25
58_Y377_Y0.5870.25
342_R345_F0.5860.25
311_I315_F0.5860.25
57_H245_E0.5830.24
205_R208_S0.5820.24
6_L223_L0.5800.24
203_K206_F0.5790.24
180_L287_L0.5790.24
122_S168_A0.5780.24
4_V27_V0.5770.24
321_S328_L0.5760.24
249_Y253_R0.5760.24
210_L216_K0.5720.24
227_A230_L0.5700.24
340_Y348_L0.5680.24
341_M353_Y0.5660.24
265_Y272_D0.5640.23
19_D22_S0.5640.23
5_A164_N0.5640.23
246_T297_L0.5640.23
157_L187_L0.5630.23
353_Y356_L0.5630.23
69_L241_E0.5630.23
48_V52_D0.5630.23
216_K328_L0.5610.23
5_A212_R0.5610.23
5_A14_F0.5600.23
311_I352_L0.5600.23
36_R55_T0.5590.23
251_L266_F0.5590.23
13_L16_S0.5580.23
199_Q206_F0.5580.23
236_L246_T0.5570.23
208_S212_R0.5560.23
199_Q207_S0.5550.23
222_L226_L0.5530.23
207_S211_L0.5530.23
8_L28_S0.5520.23
260_F264_W0.5490.23
24_R27_V0.5480.23
265_Y274_F0.5450.22
59_T232_S0.5440.22
279_L310_G0.5430.22
316_K321_S0.5410.22
212_R218_L0.5410.22
262_L267_F0.5410.22
179_S185_L0.5400.22
339_P343_Y0.5390.22
355_S362_W0.5390.22
36_R50_P0.5380.22
377_Y381_L0.5360.22
177_H239_G0.5360.22
3_A136_T0.5320.22
178_L308_L0.5320.22
277_F282_F0.5320.22
347_L359_P0.5310.22
272_D329_S0.5300.22
243_L314_I0.5300.22
164_N174_L0.5280.22
297_L303_F0.5280.22
250_H383_F0.5270.22
106_Q109_S0.5250.21
211_L215_L0.5230.21
221_F224_S0.5200.21
303_F330_L0.5200.21
332_P335_P0.5200.21
376_F382_V0.5190.21
76_I156_A0.5180.21
24_R28_S0.5180.21
14_F22_S0.5160.21
221_F228_L0.5140.21
97_L127_A0.5130.21
89_L109_S0.5130.21
202_L218_L0.5120.21
157_L186_L0.5120.21
154_L330_L0.5110.21
271_F275_R0.5100.21
89_L198_R0.5100.21
43_L274_F0.5080.21
339_P348_L0.5080.21
262_L291_L0.5050.20
21_L33_S0.5050.20
361_F383_F0.5040.20
58_Y367_Y0.5040.20
6_L162_K0.5040.20
27_V320_T0.5020.20
186_L219_L0.5010.20
280_G286_P0.5010.20
195_D221_F0.5000.20
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3rceA 1 0.8616 85.6 0.952 Contact Map
3wajA 1 0.7285 84 0.953 Contact Map
2mw3A 1 0.0548 7.1 0.976 Contact Map
1wazA 1 0.1201 3.7 0.979 Contact Map
2kv5A 1 0.0862 2.1 0.981 Contact Map
3mjoA 2 0.1749 1.4 0.983 Contact Map
2hwjA 5 0.154 1.3 0.983 Contact Map
3j1rA 5 0.0679 1.1 0.984 Contact Map
2k1eA 3 0.1201 1.1 0.984 Contact Map
3tguG 1 0.2037 1 0.984 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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