PIG-U - PFAM28P - GREMLIN Contacts

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PIG-U - GPI transamidase subunit PIG-U

PFAM:	PF06728	Sequences: LAAVALRLLLFTLFPSLPDLLSSRVEVSTPLTSYKRLTEGAFLYTHGVSPYDGGTYHYTPLLLWLLSPLLVKRPNIYLSPLFGKLLFILADLLTALLLYRILRSLQKGSSRLFKSPRKKKSSALLVAALYLFNPLTILICVRGSTSSFTNLLVLLALYLAVKGNVLLAAFALALAVHLSLYPIILLLPLLLLLLDSPRQSLLKSRFSSSLLRFFLKLLLLFLLSLLALLLLSYLLLGGGWEFLEETYGYHLTRPDLTPNFGLYWYFFYEMFDHFRSFFLGVFQLHPLIYVLPLSIRLRRDPLFAAFLLTGIFVIFKSYPTSGDFGLYLSLLPLFPHLLPYMRYSFVLLLLLLYASLLGPVFWHLWIYAGSGNANFFYAITLVF	Download Alignment We filter this alignment to remove sites that have many gaps before running GREMLIN. Date: 2015_10
Length:	383 (366)
Sequences:	700 (509)
Seq/√Len:	26.6
META:	0.091

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:

i	j	Sco	Prob
29_T	320_T	4.118	1.00
351_L	355_S	3.642	1.00
312_F	317_S	3.333	1.00
283_Q	286_P	3.167	1.00
318_Y	323_D	3.033	1.00
285_H	289_Y	2.943	1.00
316_K	323_D	2.852	1.00
221_F	225_L	2.793	1.00
152_L	174_L	2.748	1.00
4_V	92_L	2.716	0.99
358_G	362_W	2.573	0.99
355_S	359_P	2.566	0.99
318_Y	322_G	2.440	0.99
281_V	378_A	2.395	0.99
68_P	74_P	2.289	0.98
349_L	353_Y	2.265	0.98
317_S	322_G	2.249	0.98
258_P	261_G	2.232	0.98
228_L	232_S	2.220	0.98
156_A	168_A	2.219	0.98
159_L	171_A	2.183	0.97
255_D	259_N	2.064	0.96
199_Q	204_S	1.995	0.96
242_F	247_Y	1.990	0.96
265_Y	269_E	1.968	0.95
27_V	30_P	1.964	0.95
14_F	18_P	1.962	0.95
146_S	322_G	1.941	0.95
322_G	326_L	1.933	0.95
313_V	318_Y	1.927	0.95
94_T	154_L	1.926	0.95
362_W	366_I	1.912	0.95
240_W	244_E	1.911	0.95
269_E	274_F	1.897	0.94
264_W	269_E	1.863	0.94
40_G	50_P	1.854	0.94
25_V	31_L	1.828	0.93
181_Y	250_H	1.791	0.92
59_T	177_H	1.782	0.92
201_L	204_S	1.760	0.92
53_G	57_H	1.755	0.92
7_R	10_L	1.747	0.91
280_G	283_Q	1.741	0.91
33_S	36_R	1.711	0.90
41_A	45_T	1.697	0.90
350_L	353_Y	1.684	0.90
352_L	356_L	1.681	0.90
126_V	331_L	1.671	0.89
313_V	316_K	1.666	0.89
320_T	323_D	1.659	0.89
66_L	145_T	1.657	0.89
110_S	183_I	1.651	0.89
267_F	271_F	1.646	0.89
177_H	379_I	1.636	0.88
56_Y	66_L	1.635	0.88
42_F	46_H	1.632	0.88
310_G	327_Y	1.619	0.88
267_F	278_F	1.618	0.88
32_T	146_S	1.610	0.87
316_K	326_L	1.581	0.87
323_D	326_L	1.568	0.86
267_F	270_M	1.534	0.85
320_T	326_L	1.527	0.85
40_G	66_L	1.522	0.84
363_H	370_S	1.515	0.84
206_F	209_S	1.512	0.84
339_P	344_S	1.511	0.84
101_I	154_L	1.509	0.84
200_S	203_K	1.506	0.84
29_T	32_T	1.498	0.83
277_F	378_A	1.495	0.83
44_Y	373_A	1.491	0.83
95_A	161_V	1.486	0.83
325_G	329_S	1.476	0.82
217_L	221_F	1.475	0.82
180_L	191_L	1.466	0.82
319_P	323_D	1.461	0.82
351_L	358_G	1.458	0.82
143_G	378_A	1.453	0.81
44_Y	64_W	1.452	0.81
233_Y	237_G	1.451	0.81
225_L	230_L	1.451	0.81
36_R	255_D	1.439	0.81
40_G	43_L	1.433	0.80
133_N	328_L	1.431	0.80
365_W	374_N	1.423	0.80
320_T	324_F	1.421	0.80
367_Y	370_S	1.416	0.80
90_A	94_T	1.409	0.79
30_P	33_S	1.393	0.79
316_K	322_G	1.389	0.78
363_H	368_A	1.387	0.78
199_Q	202_L	1.382	0.78
365_W	372_N	1.378	0.78
336_H	339_P	1.378	0.78
164_N	170_F	1.377	0.78
225_L	229_L	1.375	0.78
185_L	309_T	1.371	0.77
274_F	278_F	1.367	0.77
177_H	300_D	1.366	0.77
318_Y	326_L	1.361	0.77
212_R	223_L	1.352	0.76
277_F	281_V	1.351	0.76
57_H	249_Y	1.350	0.76
309_T	312_F	1.338	0.76
208_S	211_L	1.336	0.75
294_S	308_L	1.334	0.75
169_A	246_T	1.333	0.75
25_V	35_K	1.332	0.75
50_P	56_Y	1.327	0.75
344_S	348_L	1.318	0.74
359_P	362_W	1.317	0.74
232_S	238_G	1.317	0.74
233_Y	242_F	1.316	0.74
198_R	202_L	1.311	0.74
345_F	367_Y	1.310	0.74
331_L	352_L	1.305	0.74
199_Q	203_K	1.304	0.74
159_L	167_L	1.302	0.74
217_L	225_L	1.295	0.73
244_E	252_T	1.292	0.73
376_F	380_T	1.291	0.73
135_L	355_S	1.279	0.72
59_T	327_Y	1.277	0.72
94_T	151_L	1.269	0.72
313_V	317_S	1.268	0.72
33_S	53_G	1.263	0.71
202_L	212_R	1.253	0.71
229_L	242_F	1.251	0.70
90_A	330_L	1.250	0.70
350_L	354_A	1.250	0.70
225_L	232_S	1.250	0.70
93_L	101_I	1.245	0.70
251_L	328_L	1.240	0.70
157_L	199_Q	1.238	0.70
27_V	31_L	1.236	0.70
176_V	221_F	1.233	0.69
250_H	317_S	1.231	0.69
52_D	241_E	1.231	0.69
216_K	220_L	1.229	0.69
98_L	138_L	1.229	0.69
213_F	216_K	1.221	0.69
40_G	63_L	1.219	0.68
221_F	227_A	1.213	0.68
137_I	140_C	1.210	0.68
282_F	286_P	1.204	0.67
91_D	95_A	1.204	0.67
269_E	277_F	1.201	0.67
90_A	251_L	1.187	0.66
317_S	323_D	1.187	0.66
324_F	383_F	1.173	0.65
201_L	206_F	1.172	0.65
217_L	220_L	1.171	0.65
182_P	250_H	1.164	0.65
173_A	239_G	1.160	0.65
256_L	278_F	1.148	0.64
69_L	74_P	1.143	0.63
218_L	224_S	1.141	0.63
272_D	275_R	1.140	0.63
234_L	238_G	1.133	0.63
222_L	225_L	1.124	0.62
249_Y	377_Y	1.121	0.62
359_P	363_H	1.118	0.62
157_L	209_S	1.117	0.62
27_V	142_R	1.116	0.61
264_W	270_M	1.113	0.61
214_F	217_L	1.113	0.61
43_L	46_H	1.113	0.61
152_L	155_L	1.106	0.61
184_I	287_L	1.103	0.61
355_S	364_L	1.103	0.61
208_S	222_L	1.097	0.60
127_A	131_L	1.092	0.60
148_F	155_L	1.087	0.59
267_F	274_F	1.081	0.59
40_G	150_N	1.080	0.59
271_F	278_F	1.079	0.59
18_P	154_L	1.073	0.58
220_L	224_S	1.067	0.58
266_F	278_F	1.067	0.58
39_E	53_G	1.063	0.58
198_R	204_S	1.057	0.57
49_S	241_E	1.054	0.57
372_N	375_F	1.053	0.57
172_L	176_V	1.045	0.56
58_Y	371_G	1.045	0.56
151_L	226_L	1.030	0.55
90_A	151_L	1.024	0.55
79_S	82_F	1.023	0.55
351_L	362_W	1.021	0.55
303_F	306_F	1.019	0.54
358_G	366_I	1.017	0.54
274_F	277_F	1.016	0.54
318_Y	324_F	1.016	0.54
160_A	192_L	1.016	0.54
354_A	358_G	1.015	0.54
375_F	380_T	1.014	0.54
185_L	317_S	1.014	0.54
148_F	352_L	1.009	0.54
182_P	259_N	1.007	0.54
340_Y	344_S	1.005	0.53
84_K	143_G	1.005	0.53
344_S	347_L	1.003	0.53
248_G	364_L	1.003	0.53
62_L	178_L	0.996	0.53
368_A	383_F	0.996	0.53
339_P	351_L	0.992	0.52
211_L	231_L	0.991	0.52
228_L	233_Y	0.990	0.52
316_K	319_P	0.990	0.52
30_P	36_R	0.989	0.52
184_I	308_L	0.988	0.52
266_F	271_F	0.984	0.52
6_L	10_L	0.980	0.52
49_S	87_F	0.979	0.51
149_T	179_S	0.966	0.50
152_L	178_L	0.966	0.50
269_E	279_L	0.961	0.50
127_A	183_I	0.960	0.50
219_L	227_A	0.957	0.50
22_S	27_V	0.955	0.50
9_L	14_F	0.955	0.50
3_A	131_L	0.954	0.50
29_T	33_S	0.951	0.49
267_F	282_F	0.951	0.49
175_A	299_R	0.949	0.49
278_F	293_L	0.949	0.49
26_E	30_P	0.944	0.49
15_P	18_P	0.938	0.48
65_L	82_F	0.938	0.48
271_F	292_P	0.938	0.48
293_L	311_I	0.934	0.48
224_S	228_L	0.934	0.48
347_L	355_S	0.933	0.48
87_F	148_F	0.932	0.48
219_L	223_L	0.923	0.47
51_Y	246_T	0.919	0.47
209_S	215_L	0.917	0.47
4_V	16_S	0.917	0.47
316_K	320_T	0.915	0.47
349_L	356_L	0.914	0.47
310_G	314_I	0.913	0.47
318_Y	321_S	0.912	0.46
153_V	183_I	0.912	0.46
278_F	315_F	0.908	0.46
266_F	269_E	0.907	0.46
201_L	205_R	0.904	0.46
5_A	9_L	0.902	0.46
96_L	168_A	0.899	0.45
221_F	226_L	0.899	0.45
338_L	343_Y	0.891	0.45
37_L	40_G	0.889	0.45
248_G	316_K	0.886	0.45
313_V	322_G	0.885	0.44
66_L	331_L	0.884	0.44
211_L	221_F	0.882	0.44
5_A	13_L	0.879	0.44
222_L	243_L	0.877	0.44
10_L	27_V	0.872	0.43
225_L	228_L	0.871	0.43
22_S	30_P	0.869	0.43
258_P	264_W	0.869	0.43
3_A	7_R	0.859	0.43
209_S	213_F	0.857	0.42
65_L	164_N	0.856	0.42
348_L	355_S	0.856	0.42
103_R	106_Q	0.854	0.42
218_L	223_L	0.853	0.42
226_L	230_L	0.852	0.42
210_L	219_L	0.851	0.42
379_I	382_V	0.844	0.41
26_E	29_T	0.841	0.41
151_L	174_L	0.837	0.41
277_F	379_I	0.837	0.41
85_L	214_F	0.836	0.41
261_G	264_W	0.834	0.41
218_L	221_F	0.834	0.41
313_V	323_D	0.833	0.41
144_S	257_T	0.832	0.41
220_L	238_G	0.832	0.41
220_L	229_L	0.830	0.40
355_S	363_H	0.829	0.40
316_K	324_F	0.827	0.40
10_L	16_S	0.826	0.40
166_L	293_L	0.825	0.40
177_H	232_S	0.824	0.40
202_L	216_K	0.824	0.40
369_G	378_A	0.821	0.40
15_P	23_S	0.820	0.40
122_S	296_R	0.815	0.39
273_H	277_F	0.815	0.39
340_Y	351_L	0.814	0.39
229_L	232_S	0.813	0.39
123_A	127_A	0.813	0.39
104_S	107_K	0.808	0.39
201_L	207_S	0.807	0.39
351_L	366_I	0.807	0.39
338_L	351_L	0.806	0.39
130_Y	150_N	0.806	0.39
341_M	344_S	0.798	0.38
258_P	292_P	0.795	0.38
49_S	143_G	0.794	0.38
68_P	75_N	0.793	0.38
275_R	278_F	0.790	0.38
138_L	346_V	0.786	0.37
8_L	13_L	0.784	0.37
9_L	111_R	0.784	0.37
51_Y	60_P	0.782	0.37
270_M	274_F	0.782	0.37
270_M	278_F	0.781	0.37
214_F	226_L	0.781	0.37
269_E	278_F	0.781	0.37
14_F	17_L	0.776	0.37
35_K	148_F	0.774	0.36
154_L	187_L	0.773	0.36
249_Y	255_D	0.772	0.36
169_A	186_L	0.771	0.36
39_E	43_L	0.771	0.36
21_L	27_V	0.770	0.36
266_F	270_M	0.770	0.36
41_A	48_V	0.768	0.36
145_T	269_E	0.764	0.36
296_R	350_L	0.759	0.35
77_Y	124_L	0.756	0.35
157_L	305_A	0.756	0.35
274_F	282_F	0.756	0.35
363_H	380_T	0.755	0.35
152_L	156_A	0.755	0.35
92_L	203_K	0.754	0.35
261_G	271_F	0.752	0.35
217_L	226_L	0.751	0.35
287_L	291_L	0.751	0.35
198_R	203_K	0.751	0.35
6_L	86_L	0.751	0.35
142_R	253_R	0.750	0.35
92_L	191_L	0.750	0.35
149_T	178_L	0.746	0.35
357_L	361_F	0.745	0.34
359_P	375_F	0.743	0.34
29_T	36_R	0.738	0.34
131_L	279_L	0.738	0.34
227_A	295_I	0.735	0.34
179_S	316_K	0.735	0.34
309_T	317_S	0.732	0.34
192_L	195_D	0.723	0.33
47_G	50_P	0.719	0.33
185_L	301_P	0.717	0.33
9_L	15_P	0.717	0.33
39_E	58_Y	0.716	0.33
3_A	8_L	0.713	0.32
86_L	219_L	0.709	0.32
230_L	295_I	0.707	0.32
168_A	243_L	0.705	0.32
180_L	332_P	0.705	0.32
113_F	120_K	0.704	0.32
228_L	238_G	0.703	0.32
346_V	357_L	0.703	0.32
52_D	338_L	0.701	0.32
332_P	362_W	0.699	0.31
273_H	362_W	0.699	0.31
198_R	205_R	0.695	0.31
13_L	18_P	0.693	0.31
216_K	225_L	0.691	0.31
198_R	201_L	0.690	0.31
277_F	342_R	0.688	0.31
218_L	225_L	0.688	0.31
294_S	311_I	0.687	0.31
139_I	324_F	0.687	0.31
216_K	227_A	0.685	0.31
39_E	142_R	0.683	0.30
141_V	256_L	0.682	0.30
339_P	355_S	0.677	0.30
345_F	350_L	0.676	0.30
350_L	361_F	0.672	0.30
183_I	230_L	0.672	0.30
322_G	325_G	0.672	0.30
92_L	206_F	0.671	0.30
142_R	378_A	0.670	0.30
94_T	152_L	0.669	0.30
361_F	379_I	0.669	0.30
368_A	380_T	0.666	0.29
220_L	228_L	0.665	0.29
100_R	207_S	0.665	0.29
41_A	66_L	0.664	0.29
358_G	361_F	0.664	0.29
281_V	284_L	0.664	0.29
271_F	285_H	0.663	0.29
229_L	299_R	0.662	0.29
139_I	142_R	0.661	0.29
278_F	292_P	0.661	0.29
39_E	323_D	0.660	0.29
271_F	277_F	0.660	0.29
277_F	288_I	0.660	0.29
31_L	36_R	0.659	0.29
371_G	382_V	0.656	0.29
287_L	292_P	0.654	0.29
3_A	14_F	0.653	0.29
45_T	225_L	0.653	0.29
224_S	229_L	0.652	0.28
180_L	293_L	0.651	0.28
93_L	190_L	0.651	0.28
271_F	274_F	0.651	0.28
285_H	377_Y	0.650	0.28
264_W	267_F	0.648	0.28
171_A	174_L	0.647	0.28
261_G	269_E	0.646	0.28
243_L	320_T	0.642	0.28
269_E	272_D	0.641	0.28
98_L	126_V	0.640	0.28
223_L	226_L	0.640	0.28
22_S	31_L	0.639	0.28
334_F	371_G	0.637	0.28
13_L	17_L	0.637	0.28
207_S	234_L	0.636	0.28
5_A	305_A	0.636	0.28
312_F	318_Y	0.635	0.27
233_Y	240_W	0.634	0.27
353_Y	361_F	0.634	0.27
128_A	295_I	0.634	0.27
319_P	322_G	0.633	0.27
229_L	236_L	0.632	0.27
210_L	214_F	0.631	0.27
256_L	275_R	0.631	0.27
353_Y	378_A	0.628	0.27
49_S	56_Y	0.627	0.27
208_S	216_K	0.625	0.27
164_N	360_V	0.625	0.27
105_L	300_D	0.625	0.27
148_F	193_L	0.624	0.27
83_G	181_Y	0.622	0.27
55_T	58_Y	0.621	0.27
63_L	373_A	0.619	0.27
264_W	274_F	0.617	0.26
145_T	264_W	0.617	0.26
233_Y	238_G	0.617	0.26
85_L	213_F	0.615	0.26
220_L	225_L	0.615	0.26
142_R	320_T	0.614	0.26
95_A	218_L	0.612	0.26
162_K	202_L	0.612	0.26
152_L	234_L	0.611	0.26
129_L	162_K	0.611	0.26
89_L	332_P	0.609	0.26
175_A	182_P	0.607	0.26
171_A	175_A	0.604	0.26
79_S	230_L	0.603	0.26
343_Y	355_S	0.603	0.26
241_E	297_L	0.600	0.25
9_L	13_L	0.600	0.25
189_L	376_F	0.598	0.25
153_V	178_L	0.597	0.25
205_R	352_L	0.595	0.25
251_L	281_V	0.594	0.25
220_L	226_L	0.594	0.25
242_F	367_Y	0.591	0.25
261_G	323_D	0.591	0.25
205_R	211_L	0.589	0.25
227_A	346_V	0.588	0.25
258_P	274_F	0.588	0.25
147_S	335_P	0.587	0.25
58_Y	377_Y	0.587	0.25
342_R	345_F	0.586	0.25
311_I	315_F	0.586	0.25
57_H	245_E	0.583	0.24
205_R	208_S	0.582	0.24
6_L	223_L	0.580	0.24
203_K	206_F	0.579	0.24
180_L	287_L	0.579	0.24
122_S	168_A	0.578	0.24
4_V	27_V	0.577	0.24
321_S	328_L	0.576	0.24
249_Y	253_R	0.576	0.24
210_L	216_K	0.572	0.24
227_A	230_L	0.570	0.24
340_Y	348_L	0.568	0.24
341_M	353_Y	0.566	0.24
265_Y	272_D	0.564	0.23
19_D	22_S	0.564	0.23
5_A	164_N	0.564	0.23
246_T	297_L	0.564	0.23
157_L	187_L	0.563	0.23
353_Y	356_L	0.563	0.23
69_L	241_E	0.563	0.23
48_V	52_D	0.563	0.23
216_K	328_L	0.561	0.23
5_A	212_R	0.561	0.23
5_A	14_F	0.560	0.23
311_I	352_L	0.560	0.23
36_R	55_T	0.559	0.23
251_L	266_F	0.559	0.23
13_L	16_S	0.558	0.23
199_Q	206_F	0.558	0.23
236_L	246_T	0.557	0.23
208_S	212_R	0.556	0.23
199_Q	207_S	0.555	0.23
222_L	226_L	0.553	0.23
207_S	211_L	0.553	0.23
8_L	28_S	0.552	0.23
260_F	264_W	0.549	0.23
24_R	27_V	0.548	0.23
265_Y	274_F	0.545	0.22
59_T	232_S	0.544	0.22
279_L	310_G	0.543	0.22
316_K	321_S	0.541	0.22
212_R	218_L	0.541	0.22
262_L	267_F	0.541	0.22
179_S	185_L	0.540	0.22
339_P	343_Y	0.539	0.22
355_S	362_W	0.539	0.22
36_R	50_P	0.538	0.22
377_Y	381_L	0.536	0.22
177_H	239_G	0.536	0.22
3_A	136_T	0.532	0.22
178_L	308_L	0.532	0.22
277_F	282_F	0.532	0.22
347_L	359_P	0.531	0.22
272_D	329_S	0.530	0.22
243_L	314_I	0.530	0.22
164_N	174_L	0.528	0.22
297_L	303_F	0.528	0.22
250_H	383_F	0.527	0.22
106_Q	109_S	0.525	0.21
211_L	215_L	0.523	0.21
221_F	224_S	0.520	0.21
303_F	330_L	0.520	0.21
332_P	335_P	0.520	0.21
376_F	382_V	0.519	0.21
76_I	156_A	0.518	0.21
24_R	28_S	0.518	0.21
14_F	22_S	0.516	0.21
221_F	228_L	0.514	0.21
97_L	127_A	0.513	0.21
89_L	109_S	0.513	0.21
202_L	218_L	0.512	0.21
157_L	186_L	0.512	0.21
154_L	330_L	0.511	0.21
271_F	275_R	0.510	0.21
89_L	198_R	0.510	0.21
43_L	274_F	0.508	0.21
339_P	348_L	0.508	0.21
262_L	291_L	0.505	0.20
21_L	33_S	0.505	0.20
361_F	383_F	0.504	0.20
58_Y	367_Y	0.504	0.20
6_L	162_K	0.504	0.20
27_V	320_T	0.502	0.20
186_L	219_L	0.501	0.20
280_G	286_P	0.501	0.20
195_D	221_F	0.500	0.20

Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

Text file of predictions (all 3L/2 predictions)
Generate restraints for ROSETTA Structure Modeling
Upload PDB model overlay contacts on the model

HHsearch Results:

Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.

PDB	Chains	Cov	%	HHΔ
3rceA	1	0.8616	85.6	0.952	Contact Map
3wajA	1	0.7285	84	0.953	Contact Map
2mw3A	1	0.0548	7.1	0.976	Contact Map
1wazA	1	0.1201	3.7	0.979	Contact Map
2kv5A	1	0.0862	2.1	0.981	Contact Map
3mjoA	2	0.1749	1.4	0.983	Contact Map
2hwjA	5	0.154	1.3	0.983	Contact Map
3j1rA	5	0.0679	1.1	0.984	Contact Map
2k1eA	3	0.1201	1.1	0.984	Contact Map
3tguG	1	0.2037	1	0.984	Contact Map

Sequence Conservation (Image Generated using WebLogo v3)

Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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