GREMLIN Database
SPC25 - Microsomal signal peptidase 25 kDa subunit (SPC25)
PFAM: PF06703 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 162 (161)
Sequences: 653 (496)
Seq/√Len: 39.1
META: 0.262

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
142_F148_L7.0741.00
104_S115_L5.0921.00
141_F156_W3.6221.00
148_L153_F3.6131.00
114_T135_T3.0161.00
104_S113_Y2.6901.00
23_H26_T2.5241.00
44_F47_D2.4791.00
7_D113_Y2.4101.00
22_D84_I2.3731.00
15_T86_F2.3221.00
13_Y19_F2.2951.00
142_F153_F2.1600.99
143_D147_V2.1400.99
3_K7_D2.1210.99
106_L138_V2.1180.99
8_D13_Y2.0670.99
32_L69_L2.0490.99
35_I69_L1.9520.99
10_V102_I1.9040.98
64_V68_I1.9020.98
51_P55_S1.8260.98
66_Y70_N1.7490.97
14_L104_S1.7330.97
78_Y82_K1.7280.97
103_S118_T1.7130.97
7_D104_S1.7070.97
53_P62_C1.6720.96
38_A42_V1.5580.94
47_D51_P1.5310.94
30_L34_Y1.5260.94
95_G98_P1.5090.93
43_A59_L1.5070.93
36_A69_L1.5050.93
85_I104_S1.4900.93
115_L157_V1.4770.93
52_F143_D1.4500.92
93_G98_P1.4360.91
154_E158_A1.4180.91
47_D55_S1.3690.89
19_F90_R1.3680.89
136_K156_W1.3460.89
45_F49_K1.3460.89
94_S98_P1.3380.88
46_Y62_C1.3160.87
102_I115_L1.3090.87
33_G70_N1.2790.86
19_F161_I1.2720.86
33_G139_T1.2620.85
21_E75_L1.2410.84
47_D66_Y1.2360.84
6_L113_Y1.2080.82
41_A44_F1.2030.82
136_K140_K1.2020.82
14_L105_S1.1980.82
2_L6_L1.1740.81
51_P54_E1.1620.80
28_V85_I1.1540.79
52_F103_S1.1350.78
92_D98_P1.1200.77
46_Y55_S1.1110.77
42_V80_V1.0960.76
52_F66_Y1.0730.74
29_R47_D1.0580.73
53_P59_L1.0580.73
36_A81_E1.0500.73
78_Y141_F1.0420.72
8_D11_K1.0200.70
84_I99_K1.0180.70
127_K130_E1.0160.70
38_A157_V1.0090.69
24_T28_V1.0080.69
22_D89_K0.9960.68
121_D126_G0.9870.68
30_L142_F0.9860.68
50_L54_E0.9820.67
2_L19_F0.9570.65
155_K159_K0.9560.65
92_D97_I0.9560.65
4_N76_Y0.9550.65
65_L72_I0.9530.65
111_P139_T0.9410.64
77_T153_F0.9280.63
3_K8_D0.9230.62
48_Y56_K0.9130.62
71_G75_L0.9120.62
9_A81_E0.9090.61
18_G117_I0.9010.61
10_V14_L0.8920.60
59_L62_C0.8880.59
11_K29_R0.8850.59
18_G82_K0.8840.59
29_R48_Y0.8720.58
32_L35_I0.8710.58
126_G129_L0.8670.58
149_V152_L0.8660.58
84_I108_K0.8630.57
87_V101_T0.8630.57
32_L72_I0.8610.57
135_T144_E0.8550.57
29_R33_G0.8520.56
85_I153_F0.8500.56
46_Y148_L0.8480.56
91_K154_E0.8480.56
131_A135_T0.8480.56
134_L141_F0.8470.56
51_P62_C0.8380.55
45_F135_T0.8180.53
39_I51_P0.8170.53
4_N8_D0.8060.52
69_L127_K0.8020.52
29_R74_T0.7970.51
36_A63_V0.7940.51
107_K145_D0.7910.51
44_F62_C0.7790.50
38_A83_G0.7730.49
68_I120_K0.7720.49
88_G100_L0.7700.49
106_L110_D0.7670.49
85_I113_Y0.7660.49
19_F86_F0.7580.48
33_G66_Y0.7560.48
133_E157_V0.7560.48
46_Y67_F0.7550.48
46_Y156_W0.7550.48
60_I85_I0.7500.47
133_E155_K0.7440.47
28_V72_I0.7420.46
6_L50_L0.7360.46
7_D69_L0.7340.46
74_T85_I0.7260.45
12_E16_S0.7250.45
101_T118_T0.7220.45
4_N70_N0.7190.44
53_P106_L0.7160.44
87_V99_K0.7160.44
57_P61_V0.7150.44
155_K161_I0.7140.44
70_N77_T0.7130.44
27_D63_V0.7130.44
26_T31_A0.7060.43
97_I129_L0.7050.43
8_D136_K0.7050.43
25_L47_D0.6990.42
2_L138_V0.6990.42
90_R101_T0.6990.42
36_A70_N0.6970.42
32_L141_F0.6960.42
75_L79_F0.6960.42
26_T33_G0.6950.42
114_T133_E0.6940.42
36_A60_I0.6930.42
39_I65_L0.6900.42
41_A151_D0.6900.42
74_T81_E0.6870.41
78_Y140_K0.6860.41
134_L159_K0.6780.41
3_K85_I0.6760.40
90_R121_D0.6740.40
49_K61_V0.6740.40
59_L148_L0.6590.39
55_S70_N0.6540.39
29_R81_E0.6530.38
32_L136_K0.6470.38
33_G40_A0.6460.38
17_L154_E0.6430.38
143_D156_W0.6380.37
1_D5_A0.6310.37
6_L106_L0.6260.36
23_H30_L0.6250.36
23_H28_V0.6190.36
66_Y108_K0.6190.36
61_V140_K0.6180.36
112_K135_T0.6150.35
49_K92_D0.6130.35
88_G99_K0.6130.35
93_G97_I0.6130.35
143_D149_V0.6110.35
15_T141_F0.6070.35
13_Y157_V0.6060.35
66_Y100_L0.6020.34
43_A81_E0.6010.34
54_E155_K0.5980.34
32_L80_V0.5980.34
1_D21_E0.5950.34
27_D73_L0.5930.33
47_D108_K0.5920.33
1_D4_N0.5910.33
45_F102_I0.5900.33
35_I123_K0.5900.33
68_I72_I0.5860.33
12_E119_Y0.5850.33
35_I145_D0.5700.32
34_Y161_I0.5690.32
2_L110_D0.5680.31
60_I64_V0.5680.31
8_D101_T0.5620.31
152_L155_K0.5590.31
140_K152_L0.5590.31
2_L154_E0.5590.31
85_I102_I0.5580.31
29_R149_V0.5560.31
86_F157_V0.5540.30
102_I116_T0.5510.30
124_S155_K0.5500.30
70_N74_T0.5480.30
27_D56_K0.5480.30
86_F90_R0.5450.30
32_L96_R0.5440.30
6_L153_F0.5440.30
15_T21_E0.5430.30
46_Y160_L0.5420.29
101_T148_L0.5410.29
89_K149_V0.5400.29
37_V83_G0.5400.29
36_A108_K0.5390.29
79_F125_G0.5380.29
90_R100_L0.5370.29
82_K115_L0.5360.29
153_F158_A0.5350.29
2_L95_G0.5300.29
57_P91_K0.5290.29
150_E154_E0.5280.28
24_T118_T0.5280.28
71_G74_T0.5250.28
116_T147_V0.5230.28
111_P137_S0.5230.28
18_G72_I0.5230.28
29_R141_F0.5230.28
38_A140_K0.5180.28
17_L64_V0.5150.27
52_F156_W0.5150.27
92_D153_F0.5140.27
6_L10_V0.5120.27
70_N83_G0.5110.27
9_A42_V0.5110.27
37_V102_I0.5100.27
19_F157_V0.5090.27
127_K131_A0.5070.27
57_P67_F0.5040.27
2_L33_G0.5000.26
93_G96_R0.5000.26
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4e17B 1 0.0926 10 0.933 Contact Map
4ev6A 3 0.4938 7.1 0.938 Contact Map
4i0uA 4 0.4815 6.6 0.939 Contact Map
4oe7A 3 0.2099 4.3 0.944 Contact Map
1oedB 1 0.1543 4.2 0.944 Contact Map
1oedA 1 0.1543 3.8 0.945 Contact Map
4akrA 1 0.9012 3.5 0.946 Contact Map
1t6cA 1 0.3889 3.3 0.947 Contact Map
3si9A 3 0.2099 3 0.948 Contact Map
4ge1A 1 0.3086 3 0.948 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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