GREMLIN Database
Tsp45I - Tsp45I type II restriction enzyme
PFAM: PF06300 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 260 (257)
Sequences: 510 (367)
Seq/√Len: 22.9
META: 0.943

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
10_Y19_D5.0971.00
95_L103_I5.0971.00
15_S19_D4.5341.00
151_G156_M4.4591.00
50_D53_E4.4401.00
71_V91_I3.9311.00
86_K146_N3.7581.00
102_K105_E3.5491.00
198_H201_A3.2341.00
10_Y15_S3.1531.00
73_Y230_I2.9370.99
248_M252_L2.9340.99
67_K112_E2.8770.99
55_I88_V2.8260.99
51_N96_Y2.7900.99
168_H204_N2.7400.99
141_L159_F2.4550.98
114_N198_H2.4470.98
68_D90_R2.3970.98
120_L123_R2.3570.97
198_H202_Q2.2960.97
122_K152_S2.2050.96
106_R109_E2.1210.95
203_F207_I2.0930.95
67_K198_H1.9820.93
194_D202_Q1.9760.93
51_N89_N1.9620.93
126_R150_D1.9500.93
67_K114_N1.9050.92
192_L201_A1.8590.91
52_I82_E1.8490.90
5_K9_E1.8360.90
168_H209_T1.8040.89
46_F99_G1.7970.89
252_L255_E1.7950.89
117_I120_L1.7840.89
55_I92_C1.7730.88
77_D83_R1.7580.88
92_C96_Y1.7380.87
67_K205_D1.7240.87
56_K78_K1.6650.85
112_E198_H1.6620.85
141_L163_H1.6450.84
254_R258_Y1.6320.84
51_N92_C1.6090.83
146_N180_F1.5950.82
122_K150_D1.5640.81
37_E138_D1.5480.80
2_Y6_L1.5320.80
70_Y191_F1.5180.79
115_R198_H1.4710.77
23_V250_A1.4650.77
227_V230_I1.4600.76
209_T220_K1.4520.76
23_V159_F1.4340.75
6_L10_Y1.4060.73
136_D139_E1.4000.73
75_K106_R1.3610.71
96_Y99_G1.3540.71
215_V220_K1.3470.70
201_A205_D1.3350.69
39_W43_E1.3340.69
61_L218_V1.3250.69
237_Y241_T1.3230.69
154_A158_D1.3150.68
41_E57_E1.3080.68
10_Y179_R1.3030.68
157_K161_K1.2850.66
206_A222_A1.2660.65
78_K238_K1.2490.64
112_E205_D1.2330.63
72_A192_L1.2310.63
56_K82_E1.2290.63
194_D201_A1.2280.63
144_K182_G1.2210.62
39_W104_Y1.2100.62
3_W6_L1.1820.60
132_I148_I1.1780.60
42_I103_I1.1740.59
201_A204_N1.1690.59
28_P31_I1.1550.58
42_I57_E1.1470.57
122_K156_M1.1430.57
172_K205_D1.1290.56
194_D198_H1.1290.56
204_N207_I1.1290.56
168_H171_N1.1280.56
178_A257_L1.1250.56
247_I256_F1.1240.56
49_K96_Y1.1170.55
113_T116_Q1.1110.55
185_V260_L1.1070.55
52_I56_K1.1070.55
249_S253_L1.1030.54
158_D162_E1.0920.54
72_A200_N1.0900.54
72_A194_D1.0770.53
7_S11_A1.0770.53
47_N184_Y1.0720.52
29_E34_I1.0720.52
195_F198_H1.0530.51
153_D173_G1.0500.51
76_R194_D1.0410.50
69_S113_T1.0360.50
48_K53_E1.0250.49
44_K50_D1.0210.49
168_H208_S1.0190.49
23_V142_K1.0130.48
42_I50_D1.0070.48
122_K176_F1.0050.48
193_T202_Q1.0020.47
153_D174_L1.0010.47
73_Y76_R1.0000.47
26_T113_T1.0000.47
132_I179_R0.9980.47
127_K193_T0.9930.47
209_T212_A0.9900.47
93_G180_F0.9880.46
29_E94_R0.9870.46
129_S132_I0.9820.46
42_I95_L0.9790.46
52_I92_C0.9770.46
75_K167_K0.9710.45
234_N240_I0.9700.45
182_G189_A0.9590.44
211_K245_Y0.9580.44
198_H205_D0.9570.44
172_K190_K0.9530.44
161_K168_H0.9530.44
211_K244_D0.9440.43
76_R195_F0.9360.43
132_I180_F0.9360.43
16_Y24_Y0.9350.43
71_V184_Y0.9290.42
35_D39_W0.9290.42
98_M137_L0.9260.42
35_D43_E0.9260.42
86_K89_N0.9220.42
72_A201_A0.9200.42
147_A179_R0.9170.42
52_I101_D0.9130.41
18_D24_Y0.9130.41
138_D185_V0.9110.41
60_K78_K0.9090.41
82_E177_V0.9070.41
115_R222_A0.9070.41
138_D143_T0.8970.40
72_A236_M0.8770.39
155_E163_H0.8760.39
115_R202_Q0.8650.38
146_N182_G0.8590.38
68_D72_A0.8530.37
81_I88_V0.8490.37
143_T165_G0.8480.37
57_E60_K0.8470.37
44_K53_E0.8420.37
42_I61_L0.8410.37
63_L69_S0.8400.37
195_F201_A0.8370.36
79_S82_E0.8360.36
205_D209_T0.8350.36
75_K80_A0.8330.36
203_F228_L0.8320.36
208_S260_L0.8300.36
87_T90_R0.8260.36
10_Y26_T0.8230.35
2_Y14_R0.8210.35
125_L163_H0.8200.35
34_I61_L0.8190.35
211_K221_I0.8180.35
239_D242_E0.8170.35
43_E100_L0.8170.35
202_Q236_M0.8170.35
29_E116_Q0.8150.35
6_L27_I0.8140.35
57_E88_V0.8130.35
122_K125_L0.8110.35
253_L256_F0.8070.34
91_I213_K0.8070.34
91_I155_E0.8040.34
93_G254_R0.8010.34
154_A233_N0.8000.34
135_V147_A0.7960.34
45_A182_G0.7960.34
46_F96_Y0.7930.34
48_K138_D0.7930.34
204_N208_S0.7920.34
63_L229_Y0.7920.34
149_L251_L0.7900.33
2_Y5_K0.7860.33
142_K145_G0.7860.33
193_T198_H0.7830.33
132_I135_V0.7800.33
79_S165_G0.7770.33
89_N93_G0.7770.33
134_P137_L0.7740.32
4_T213_K0.7710.32
99_G102_K0.7660.32
43_E71_V0.7630.32
9_E36_E0.7500.31
62_D145_G0.7470.31
59_L75_K0.7470.31
46_F51_N0.7440.31
49_K216_K0.7420.30
192_L205_D0.7420.30
146_N179_R0.7410.30
80_A227_V0.7400.30
157_K171_N0.7390.30
116_Q202_Q0.7340.30
248_M259_Q0.7320.30
91_I120_L0.7310.30
100_L108_S0.7290.30
45_A95_L0.7290.30
198_H222_A0.7280.30
82_E158_D0.7260.30
37_E40_K0.7240.29
88_V120_L0.7210.29
53_E185_V0.7200.29
251_L256_F0.7190.29
36_E47_N0.7170.29
192_L202_Q0.7160.29
149_L177_V0.7140.29
41_E143_T0.7140.29
86_K145_G0.7110.29
140_F183_K0.7080.29
214_N217_A0.7080.29
241_T246_N0.7070.28
33_E82_E0.7060.28
8_I12_N0.7040.28
135_V248_M0.7030.28
40_K139_E0.6970.28
100_L103_I0.6960.28
114_N192_L0.6930.28
39_W42_I0.6890.27
128_K254_R0.6890.27
2_Y123_R0.6880.27
4_T7_S0.6870.27
19_D184_Y0.6870.27
218_V260_L0.6870.27
1_N18_D0.6840.27
224_L228_L0.6830.27
136_D150_D0.6830.27
19_D26_T0.6820.27
20_L24_Y0.6810.27
148_I183_K0.6790.27
117_I235_K0.6780.27
67_K113_T0.6740.27
145_G163_H0.6740.27
234_N238_K0.6730.27
72_A202_Q0.6710.26
75_K117_I0.6680.26
38_I60_K0.6670.26
4_T8_I0.6650.26
165_G177_V0.6630.26
149_L156_M0.6610.26
111_K203_F0.6610.26
52_I81_I0.6610.26
26_T30_G0.6600.26
73_Y83_R0.6600.26
38_I41_E0.6580.26
26_T69_S0.6570.26
93_G97_E0.6550.26
41_E47_N0.6540.26
8_I11_A0.6510.25
168_H207_I0.6470.25
9_E233_N0.6440.25
53_E182_G0.6410.25
174_L177_V0.6380.25
72_A76_R0.6340.25
82_E127_K0.6340.25
161_K167_K0.6330.24
46_F88_V0.6320.24
17_L24_Y0.6310.24
203_F206_A0.6300.24
146_N163_H0.6270.24
42_I100_L0.6240.24
53_E142_K0.6240.24
238_K242_E0.6230.24
41_E44_K0.6210.24
55_I58_L0.6190.24
202_Q206_A0.6190.24
4_T19_D0.6190.24
192_L195_F0.6190.24
19_D27_I0.6180.24
36_E135_V0.6170.24
27_I31_I0.6150.24
29_E92_C0.6130.23
55_I216_K0.6130.23
112_E192_L0.6130.23
117_I198_H0.6120.23
55_I145_G0.6100.23
63_L193_T0.6080.23
125_L216_K0.6080.23
93_G258_Y0.6040.23
248_M255_E0.6030.23
7_S116_Q0.6020.23
34_I43_E0.6010.23
207_I233_N0.6000.23
51_N134_P0.5990.23
23_V126_R0.5970.23
142_K211_K0.5960.23
72_A195_F0.5910.22
112_E190_K0.5910.22
201_A235_K0.5900.22
156_M176_F0.5900.22
44_K177_V0.5890.22
88_V244_D0.5880.22
253_L257_L0.5880.22
139_E208_S0.5870.22
19_D22_Q0.5860.22
129_S133_P0.5850.22
200_N235_K0.5850.22
114_N185_V0.5840.22
1_N7_S0.5820.22
192_L200_N0.5800.22
160_A177_V0.5790.22
30_G252_L0.5780.22
196_G199_Q0.5750.22
22_Q165_G0.5740.22
135_V143_T0.5690.21
126_R163_H0.5690.21
36_E40_K0.5680.21
100_L104_Y0.5670.21
195_F202_Q0.5670.21
190_K202_Q0.5660.21
156_M210_I0.5660.21
207_I239_D0.5650.21
41_E60_K0.5650.21
142_K213_K0.5620.21
60_K139_E0.5610.21
95_L98_M0.5610.21
55_I74_L0.5600.21
211_K243_K0.5590.21
120_L252_L0.5580.21
137_L151_G0.5540.21
94_R147_A0.5540.21
49_K213_K0.5530.21
77_D159_F0.5500.20
34_I177_V0.5490.20
134_P180_F0.5490.20
48_K144_K0.5480.20
34_I255_E0.5420.20
78_K161_K0.5400.20
66_I115_R0.5400.20
3_W109_E0.5390.20
16_Y20_L0.5350.20
3_W12_N0.5340.20
60_K239_D0.5340.20
3_W7_S0.5330.20
69_S83_R0.5330.20
61_L107_C0.5330.20
45_A106_R0.5310.20
89_N244_D0.5310.20
52_I80_A0.5300.20
246_N256_F0.5280.20
239_D245_Y0.5240.19
35_D40_K0.5240.19
82_E182_G0.5200.19
102_K142_K0.5200.19
4_T15_S0.5180.19
40_K48_K0.5180.19
2_Y10_Y0.5170.19
194_D200_N0.5170.19
51_N93_G0.5170.19
1_N16_Y0.5170.19
68_D94_R0.5150.19
39_W179_R0.5150.19
180_F258_Y0.5150.19
201_A206_A0.5140.19
4_T45_A0.5140.19
176_F223_I0.5140.19
114_N247_I0.5140.19
80_A83_R0.5130.19
73_Y80_A0.5120.19
6_L9_E0.5100.19
166_Y174_L0.5080.19
102_K158_D0.5080.19
246_N252_L0.5070.19
53_E56_K0.5060.19
86_K240_I0.5050.19
48_K137_L0.5050.19
189_A217_A0.5050.19
195_F234_N0.5030.18
19_D128_K0.5030.18
91_I239_D0.5000.18
3_W95_L0.5000.18
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4f42A 1 0.2923 59.2 0.962 Contact Map
2ib1A 1 0.2962 38.4 0.966 Contact Map
4cu5A 2 0.2654 35 0.967 Contact Map
2dbfA 1 0.3423 24.5 0.969 Contact Map
4rvqA 1 0.4 15.1 0.972 Contact Map
2d96A 1 0.3769 12.4 0.973 Contact Map
2g7rA 1 0.2769 11.4 0.974 Contact Map
1hh1A 2 0.4 11 0.974 Contact Map
1ob8A 1 0.4038 8.8 0.975 Contact Map
3mopK 3 0.3077 7.4 0.976 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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