GREMLIN Database
CobT - Cobalamin biosynthesis protein CobT
PFAM: PF06213 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 276 (268)
Sequences: 5710 (4129)
Seq/√Len: 252.2
META: 0.856

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
114_D152_R3.7611.00
186_D189_A3.7551.00
148_R178_D3.2391.00
15_A40_A3.1111.00
93_L145_V3.0981.00
21_D68_L3.0401.00
159_R163_D2.9981.00
62_D112_N2.9721.00
147_E166_R2.9581.00
46_P53_E2.8271.00
70_H105_R2.6771.00
89_V141_L2.6641.00
18_I109_V2.5961.00
80_A87_R2.5801.00
35_L40_A2.4811.00
110_A152_R2.3361.00
156_P159_R2.2581.00
170_E174_G2.2351.00
15_A61_A2.0681.00
145_V149_L2.0621.00
11_L15_A2.0461.00
63_S95_Q1.9921.00
242_E245_E1.9801.00
43_P60_E1.9601.00
264_V267_D1.8561.00
176_D200_D1.8431.00
196_D199_E1.8081.00
129_I135_A1.8051.00
248_E252_S1.7851.00
173_A197_L1.7631.00
163_D167_P1.7611.00
265_E269_A1.7361.00
118_E157_A1.7171.00
248_E251_E1.7151.00
223_E226_D1.6961.00
135_A161_V1.6471.00
20_G69_R1.6471.00
199_E204_G1.6281.00
209_D212_D1.6041.00
168_W171_E1.6021.00
239_E248_E1.5981.00
21_D38_K1.5911.00
51_P55_A1.5881.00
58_R112_N1.5841.00
245_E248_E1.5621.00
118_E122_R1.5601.00
244_S247_A1.5481.00
244_S248_E1.5441.00
49_L54_V1.5301.00
147_E162_V1.5211.00
260_E264_V1.5201.00
67_R98_V1.5161.00
252_S256_D1.5131.00
233_D236_G1.5101.00
224_E228_E1.5061.00
63_S98_V1.4991.00
7_F11_L1.4781.00
140_A165_W1.4711.00
80_A91_D1.4701.00
93_L194_A1.4671.00
246_E249_S1.4631.00
187_Q190_F1.4631.00
241_E244_S1.4341.00
58_R62_D1.4321.00
196_D200_D1.4231.00
154_P162_V1.4211.00
244_S249_S1.4201.00
111_A152_R1.4201.00
247_A250_E1.4201.00
259_E262_G1.4121.00
179_A182_D1.4001.00
263_E266_A1.3851.00
224_E227_D1.3721.00
27_S39_K1.3701.00
66_L102_G1.3671.00
153_P159_R1.3671.00
66_L109_V1.3591.00
195_R204_G1.3451.00
266_A269_A1.3401.00
81_P90_F1.3331.00
7_F54_V1.3321.00
267_D270_A1.3291.00
249_S252_S1.3291.00
254_E257_D1.3121.00
88_A138_A1.3061.00
251_E254_E1.2881.00
83_G198_L1.2881.00
147_E153_P1.2841.00
162_V169_I1.2831.00
131_D134_D1.2781.00
121_Y126_Y1.2631.00
12_A28_F1.2421.00
226_D229_E1.2221.00
67_R95_Q1.2181.00
35_L64_L1.2131.00
90_F191_A1.2051.00
76_H91_D1.2001.00
71_H76_H1.1981.00
168_W172_K1.1931.00
261_E264_V1.1891.00
95_Q99_E1.1821.00
119_D123_R1.1761.00
50_T53_E1.1671.00
243_E246_E1.1601.00
27_S41_R1.1571.00
222_E225_E1.1561.00
241_E249_S1.1521.00
262_G266_A1.1511.00
244_S252_S1.1471.00
15_A24_L1.1421.00
166_R170_E1.1341.00
236_G242_E1.1271.00
243_E248_E1.1191.00
262_G269_A1.1071.00
33_P64_L1.1071.00
73_A77_A1.1061.00
141_L198_L1.1051.00
106_M109_V1.1051.00
116_M120_R1.1041.00
252_S255_E1.1031.00
10_A13_A1.0961.00
240_D245_E1.0841.00
247_A251_E1.0801.00
238_D251_E1.0791.00
114_D118_E1.0781.00
259_E266_A1.0781.00
250_E253_E1.0741.00
183_A193_L1.0741.00
246_E257_D1.0651.00
83_G86_A1.0581.00
261_E267_D1.0541.00
252_S257_D1.0491.00
260_E266_A1.0471.00
225_E228_E1.0341.00
79_L188_A1.0321.00
247_A252_S1.0281.00
243_E247_A1.0261.00
183_A189_A1.0171.00
24_L38_K1.0091.00
95_Q98_V1.0031.00
104_R185_E0.9981.00
139_E142_A0.9941.00
124_K139_E0.9941.00
167_P171_E0.9901.00
194_A197_L0.9881.00
126_Y129_I0.9861.00
78_R188_A0.9841.00
245_E249_S0.9831.00
253_E256_D0.9801.00
245_E250_E0.9801.00
250_E254_E0.9771.00
148_R177_L0.9701.00
234_E247_A0.9661.00
110_A114_D0.9611.00
239_E242_E0.9541.00
236_G239_E0.9541.00
92_A96_A0.9481.00
79_L90_F0.9481.00
60_E64_L0.9341.00
259_E263_E0.9311.00
255_E264_V0.9241.00
5_E9_R0.9241.00
245_E252_S0.9231.00
179_A193_L0.9191.00
188_A192_R0.9141.00
179_A196_D0.9131.00
206_E209_D0.9081.00
56_I119_D0.9061.00
236_G244_S0.9041.00
101_L105_R0.9041.00
189_A192_R0.9001.00
237_E241_E0.8921.00
241_E246_E0.8901.00
63_S99_E0.8771.00
103_A113_L0.8691.00
219_E223_E0.8671.00
126_Y161_V0.8650.99
8_K15_A0.8620.99
24_L68_L0.8610.99
36_S39_K0.8580.99
244_S250_E0.8540.99
242_E248_E0.8540.99
263_E267_D0.8460.99
247_A254_E0.8440.99
247_A257_D0.8420.99
240_D254_E0.8400.99
241_E254_E0.8360.99
248_E253_E0.8350.99
129_I161_V0.8340.99
75_L79_L0.8290.99
260_E263_E0.8290.99
125_G128_R0.8280.99
66_L98_V0.8260.99
228_E231_D0.8250.99
249_S255_E0.8230.99
262_G270_A0.8220.99
237_E240_D0.8180.99
258_D262_G0.8180.99
63_S116_M0.8100.99
211_P214_E0.8070.99
100_A150_T0.8070.99
172_K175_A0.8060.99
239_E247_A0.8040.99
132_R168_W0.8030.99
119_D122_R0.8030.99
121_Y143_L0.8010.99
81_P86_A0.8010.99
236_G248_E0.7990.99
261_E270_A0.7980.99
3_P6_P0.7960.99
45_P49_L0.7950.99
19_A22_P0.7930.99
40_A64_L0.7900.99
247_A256_D0.7880.99
251_E255_E0.7880.99
238_D241_E0.7840.99
245_E257_D0.7830.99
246_E250_E0.7830.99
107_P111_A0.7830.99
243_E249_S0.7790.99
138_A142_A0.7770.99
264_V270_A0.7700.99
215_D218_E0.7700.99
240_D248_E0.7670.99
179_A183_A0.7630.99
93_L197_L0.7550.99
97_R101_L0.7540.99
132_R135_A0.7520.99
236_G243_E0.7510.99
253_E257_D0.7460.99
262_G267_D0.7420.98
240_D244_S0.7420.98
6_P9_R0.7420.98
223_E227_D0.7420.98
250_E256_D0.7410.98
235_E248_E0.7370.98
246_E252_S0.7370.98
266_A270_A0.7360.98
204_G207_L0.7300.98
145_V190_F0.7300.98
234_E238_D0.7270.98
251_E257_D0.7270.98
235_E249_S0.7260.98
241_E245_E0.7240.98
136_P164_L0.7240.98
197_L200_D0.7180.98
262_G265_E0.7160.98
107_P110_A0.7160.98
30_A34_G0.7150.98
241_E250_E0.7140.98
237_E250_E0.7110.98
11_L57_A0.7110.98
240_D269_A0.7070.98
263_E269_A0.7060.98
240_D257_D0.7060.98
198_L204_G0.7010.98
222_E228_E0.7000.98
133_E136_P0.6990.98
249_S256_D0.6990.98
250_E258_D0.6970.98
140_A161_V0.6960.98
235_E245_E0.6950.98
167_P170_E0.6910.98
243_E254_E0.6900.98
225_E229_E0.6870.97
90_F198_L0.6870.97
239_E243_E0.6850.97
237_E251_E0.6820.97
241_E251_E0.6810.97
244_S254_E0.6810.97
144_L166_R0.6800.97
79_L191_A0.6760.97
260_E269_A0.6750.97
265_E268_D0.6710.97
247_A258_D0.6710.97
58_R187_Q0.6700.97
241_E247_A0.6700.97
117_L121_Y0.6660.97
8_K45_P0.6650.97
215_D224_E0.6650.97
243_E259_E0.6640.97
15_A64_L0.6630.97
140_A162_V0.6620.97
260_E270_A0.6590.97
24_L40_A0.6580.97
26_V33_P0.6570.97
239_E244_S0.6570.97
252_S268_D0.6560.97
257_D261_E0.6540.97
9_R29_G0.6530.97
132_R164_L0.6510.96
101_L184_L0.6480.96
79_L192_R0.6470.96
248_E263_E0.6470.96
144_L169_I0.6460.96
15_A28_F0.6340.96
240_D243_E0.6320.96
81_P187_Q0.6320.96
234_E250_E0.6310.96
6_P10_A0.6280.96
111_A115_A0.6240.96
261_E265_E0.6240.96
239_E262_G0.6240.96
62_D108_G0.6220.95
34_G41_R0.6170.95
79_L207_L0.6160.95
35_L68_L0.6160.95
121_Y125_G0.6160.95
101_L185_E0.6150.95
233_D237_E0.6150.95
14_A61_A0.6150.95
185_E188_A0.6150.95
252_S263_E0.6140.95
109_V113_L0.6120.95
18_I66_L0.6120.95
220_E226_D0.6110.95
259_E270_A0.6090.95
60_E63_S0.6080.95
254_E258_D0.6030.95
108_G112_N0.6020.95
169_I173_A0.6020.95
80_A90_F0.6000.95
259_E269_A0.5990.94
149_L184_L0.5990.94
249_S258_D0.5980.94
245_E254_E0.5980.94
247_A253_E0.5970.94
182_D185_E0.5960.94
160_K167_P0.5960.94
241_E248_E0.5930.94
150_T154_P0.5930.94
237_E249_S0.5910.94
206_E212_D0.5900.94
194_A198_L0.5880.94
233_D244_S0.5880.94
13_A17_A0.5880.94
132_R165_W0.5860.94
236_G240_D0.5840.94
112_N187_Q0.5840.94
260_E267_D0.5810.94
219_E229_E0.5790.93
238_D242_E0.5790.93
264_V269_A0.5760.93
246_E254_E0.5750.93
121_Y158_A0.5750.93
261_E266_A0.5740.93
254_E263_E0.5740.93
169_I183_A0.5720.93
176_D180_L0.5720.93
243_E251_E0.5710.93
184_L194_A0.5710.93
238_D246_E0.5690.93
253_E258_D0.5690.93
249_S254_E0.5670.93
83_G204_G0.5610.92
125_G129_I0.5600.92
235_E240_D0.5570.92
144_L162_V0.5570.92
121_Y142_A0.5550.92
258_D267_D0.5550.92
252_S258_D0.5530.92
7_F57_A0.5530.92
265_E270_A0.5530.92
5_E8_K0.5520.92
91_D95_Q0.5510.92
260_E265_E0.5480.91
238_D247_A0.5440.91
84_P88_A0.5440.91
242_E247_A0.5420.91
206_E210_D0.5400.91
175_A178_D0.5390.91
259_E268_D0.5380.91
245_E251_E0.5370.91
243_E256_D0.5340.90
121_Y154_P0.5340.90
95_Q120_R0.5330.90
104_R181_A0.5320.90
249_S261_E0.5300.90
33_P47_R0.5280.90
253_E262_G0.5260.90
104_R184_L0.5250.90
163_D166_R0.5250.90
246_E253_E0.5230.89
162_V166_R0.5210.89
113_L117_L0.5210.89
19_A69_R0.5200.89
11_L61_A0.5200.89
220_E224_E0.5170.89
29_G32_P0.5160.89
21_D24_L0.5160.89
30_A47_R0.5150.89
259_E264_V0.5150.89
242_E246_E0.5140.89
106_M124_K0.5140.89
120_R139_E0.5120.88
248_E256_D0.5110.88
89_V92_A0.5080.88
237_E248_E0.5070.88
234_E240_D0.5060.88
252_S266_A0.5050.88
241_E264_V0.5040.88
236_G247_A0.5000.87
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4u7aA 1 0 91.7 0.903 Contact Map
4qhjA 2 0.337 71.1 0.925 Contact Map
3cqbA 4 0.2826 67.6 0.926 Contact Map
4jixA 2 0.3696 32.2 0.941 Contact Map
4qu4A 1 0 19.2 0.947 Contact Map
4fp9B 2 0.6341 12.2 0.951 Contact Map
4jiuA 1 0.3623 10.3 0.953 Contact Map
4kblA 2 0 9 0.954 Contact Map
2yhsA 1 0.192 7.8 0.956 Contact Map
3mlfA 2 0.3442 7.1 0.956 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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