GREMLIN Database
DUF996 - Protein of unknown function (DUF996)
PFAM: PF06195 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 135 (134)
Sequences: 1530 (1257)
Seq/√Len: 108.6
META: 0.932

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
14_K18_D5.0881.00
112_L116_I3.8891.00
92_R96_N3.6661.00
22_D101_K3.5381.00
23_P27_N3.0231.00
28_N93_K2.5881.00
89_I115_L2.5361.00
22_D97_L2.4811.00
20_Y102_S2.4481.00
109_T113_L2.3991.00
115_L119_I2.3661.00
13_L29_A2.2861.00
114_Y133_A2.1481.00
16_L102_S1.9581.00
4_V8_L1.7821.00
121_T127_L1.7461.00
13_L98_L1.7461.00
105_G108_K1.6811.00
99_A108_K1.6731.00
24_S97_L1.6671.00
100_E108_K1.6051.00
10_L33_F1.5881.00
26_F30_L1.5771.00
20_Y98_L1.5331.00
112_L115_L1.5281.00
88_A118_A1.5221.00
44_V48_L1.4821.00
120_L123_I1.4721.00
62_G65_G1.4721.00
81_W85_I1.4631.00
45_V49_A1.4081.00
35_L83_F1.3681.00
16_L98_L1.3110.99
96_N108_K1.3100.99
124_L128_I1.2950.99
62_G67_S1.2940.99
58_L62_G1.2870.99
64_T68_S1.2800.99
127_L130_I1.2750.99
91_L94_S1.2530.99
62_G66_W1.2340.99
99_A102_S1.2170.99
9_V13_L1.2010.99
25_I28_N1.1990.99
30_L34_I1.1960.99
24_S28_N1.1920.99
70_I73_I1.1690.99
63_P67_S1.1620.99
55_L58_L1.1380.98
50_A57_G1.1370.98
16_L20_Y1.1370.98
36_G87_S1.1350.98
4_V7_I1.1300.98
96_N112_L1.1260.98
111_G115_L1.1220.98
89_I93_K1.0880.98
13_L95_F1.0820.98
15_G19_Y1.0690.98
54_L57_G1.0340.97
109_T112_L1.0170.97
53_S56_S1.0040.97
43_A80_A1.0020.96
32_G91_L0.9950.96
86_I90_F0.9890.96
29_A95_F0.9830.96
7_I11_I0.9630.96
31_Y90_F0.9610.96
63_P125_I0.9530.95
37_I41_V0.9510.95
59_G63_P0.9320.95
106_L110_A0.9210.94
14_K26_F0.9090.94
12_A19_Y0.9060.94
88_A133_A0.9040.94
64_T67_S0.9010.94
113_L116_I0.8960.93
98_L102_S0.8940.93
17_S23_P0.8870.93
90_F94_S0.8840.93
110_A114_Y0.8810.93
54_L61_T0.8780.93
81_W98_L0.8760.93
29_A94_S0.8710.93
50_A54_L0.8670.92
32_G87_S0.8660.92
90_F112_L0.8650.92
94_S98_L0.8650.92
58_L63_P0.8530.92
117_G126_G0.8470.91
51_F54_L0.8420.91
50_A76_A0.8380.91
3_I7_I0.8380.91
46_V50_A0.8290.91
53_S60_F0.8240.90
17_S25_I0.8190.90
104_V109_T0.8160.90
14_K17_S0.8070.89
70_I74_I0.8000.89
87_S91_L0.7960.89
35_L86_I0.7900.88
57_G61_T0.7890.88
61_T64_T0.7880.88
60_F63_P0.7870.88
77_L125_I0.7860.88
30_L47_V0.7850.88
2_S6_L0.7830.88
74_I125_I0.7710.87
112_L119_I0.7700.87
52_F57_G0.7680.87
63_P66_W0.7660.87
78_V82_V0.7650.87
33_F91_L0.7640.87
85_I119_I0.7600.86
25_I94_S0.7530.86
33_F47_V0.7520.86
85_I89_I0.7510.86
9_V12_A0.7460.85
95_F134_W0.7420.85
82_V86_I0.7400.85
3_I16_L0.7370.85
51_F55_L0.7320.84
49_A53_S0.7190.83
35_L90_F0.7170.83
124_L127_L0.7140.83
62_G68_S0.7020.82
51_F64_T0.7010.82
61_T66_W0.6950.81
38_V109_T0.6850.80
50_A53_S0.6820.80
128_I135_I0.6780.79
69_L78_V0.6760.79
57_G67_S0.6730.79
31_Y35_L0.6710.79
52_F56_S0.6700.79
96_N119_I0.6690.78
95_F99_A0.6630.78
64_T74_I0.6610.78
11_I15_G0.6550.77
52_F55_L0.6540.77
15_G18_D0.6540.77
44_V49_A0.6500.76
10_L26_F0.6470.76
107_F110_A0.6460.76
89_I102_S0.6440.76
87_S114_Y0.6410.75
65_G68_S0.6310.74
39_G83_F0.6310.74
121_T134_W0.6250.74
55_L59_G0.6250.74
61_T65_G0.6250.74
129_L133_A0.6200.73
69_L72_G0.6200.73
35_L43_A0.6190.73
95_F98_L0.6170.73
68_S124_L0.6110.72
88_A115_L0.6060.71
105_G109_T0.6020.71
47_V55_L0.5990.70
16_L106_L0.5950.70
52_F59_G0.5890.69
49_A58_L0.5890.69
10_L29_A0.5880.69
45_V62_G0.5790.68
40_I84_L0.5770.68
116_I119_I0.5760.67
10_L32_G0.5720.67
56_S62_G0.5670.66
131_F135_I0.5670.66
50_A55_L0.5640.66
81_W91_L0.5630.66
94_S108_K0.5620.66
109_T116_I0.5610.65
32_G88_A0.5600.65
53_S57_G0.5580.65
17_S27_N0.5570.65
95_F120_L0.5540.65
69_L75_V0.5500.64
45_V53_S0.5490.64
22_D122_I0.5470.64
53_S68_S0.5430.63
60_F64_T0.5430.63
12_A15_G0.5420.63
60_F65_G0.5400.63
81_W121_T0.5390.62
77_L81_W0.5350.62
52_F58_L0.5340.62
6_L12_A0.5330.62
59_G64_T0.5320.61
56_S59_G0.5290.61
23_P103_G0.5280.61
10_L14_K0.5240.60
28_N31_Y0.5230.60
40_I53_S0.5180.59
109_T131_F0.5130.59
31_Y34_I0.5120.59
47_V50_A0.5100.58
5_G13_L0.5100.58
95_F114_Y0.5100.58
9_V14_K0.5050.57
120_L126_G0.5020.57
93_K111_G0.5000.57
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3j1zP 8 0.9481 37.4 0.874 Contact Map
3h90A 3 0.9481 28.8 0.881 Contact Map
3x29A 1 0.6444 5.1 0.916 Contact Map
4lz6A 1 0.8519 3.5 0.922 Contact Map
2nq2A 2 0.9185 3.3 0.923 Contact Map
4p79A 1 0.7111 3.1 0.924 Contact Map
3v5uA 1 0.4963 2.9 0.925 Contact Map
4g1uA 2 0.9333 2.9 0.925 Contact Map
2qi9A 2 0.9259 2.2 0.93 Contact Map
3w4tA 1 0.8 1.5 0.936 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0063 seconds.