GREMLIN Database
EcsB - Bacterial ABC transporter protein EcsB
PFAM: PF05975 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 383 (380)
Sequences: 1076 (864)
Seq/√Len: 44.3
META: 0.262

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
315_T357_Q7.5741.00
75_L101_Y5.0161.00
284_Y353_L4.8431.00
247_R252_D4.2721.00
273_T326_H4.1981.00
42_Y46_L3.3731.00
66_L113_V3.0501.00
270_Y342_R3.0391.00
291_G361_F2.7851.00
292_A360_L2.7381.00
282_G286_R2.7151.00
255_L272_R2.6891.00
60_A64_G2.6591.00
288_T357_Q2.6301.00
92_K96_K2.6121.00
106_Q143_K2.5121.00
316_G354_L2.4571.00
274_F349_L2.4421.00
175_L179_L2.4081.00
70_G143_K2.3621.00
265_T337_V2.3451.00
146_N173_N2.3431.00
73_R101_Y2.3051.00
61_L367_I2.3031.00
2_E6_K2.2771.00
73_R221_E2.2671.00
78_E222_Q2.2331.00
264_N348_K2.2061.00
70_G147_L2.1991.00
252_D272_R2.1191.00
23_F26_H2.1111.00
326_H331_W2.0880.99
35_L285_L2.0560.99
22_V232_A2.0100.99
205_R208_K1.9960.99
259_P264_N1.9870.99
30_V33_F1.9730.99
63_L135_L1.9650.99
288_T315_T1.9520.99
59_L63_L1.9320.99
26_H36_G1.8960.99
107_L140_L1.8750.99
25_D30_V1.8530.99
291_G311_F1.8500.99
21_Y33_F1.8430.99
4_F98_A1.8110.98
249_K252_D1.8040.98
93_G97_R1.7890.98
212_L217_L1.7860.98
11_H101_Y1.7760.98
59_L121_Y1.7610.98
59_L117_L1.7610.98
64_G174_A1.7500.98
24_N30_V1.7230.98
4_F8_L1.7080.98
292_A364_V1.7040.98
255_L275_L1.7000.98
78_E81_L1.6880.98
137_L186_L1.6460.97
110_L139_L1.6320.97
26_H30_V1.6300.97
233_N237_D1.6190.97
264_N267_L1.6180.97
22_V27_F1.6100.97
286_R318_Q1.6070.97
258_I271_L1.5890.96
178_Y182_S1.5850.96
103_F275_L1.5780.96
214_W218_I1.5680.96
280_Y322_L1.5570.96
100_R358_A1.5560.96
73_R224_R1.5550.96
68_T173_N1.5460.96
77_K331_W1.5430.96
74_T94_Y1.5200.95
179_L184_A1.5110.95
8_L29_L1.5040.95
4_F101_Y1.4970.95
8_L101_Y1.4870.95
285_L289_V1.4800.95
18_Y21_Y1.4660.94
21_Y236_T1.4640.94
85_L91_M1.4600.94
80_D276_R1.4590.94
6_K10_A1.4540.94
318_Q336_P1.4260.94
18_Y318_Q1.4150.93
185_P190_L1.3970.93
38_G223_K1.3790.92
21_Y24_N1.3780.92
22_V228_F1.3740.92
27_F30_V1.3630.92
114_L136_L1.3620.92
39_A283_L1.3620.92
137_L177_L1.3550.92
76_L218_I1.3540.92
228_F232_A1.3520.92
321_P346_F1.3290.91
14_E225_M1.3270.91
252_D255_L1.3260.91
312_L381_G1.3210.91
200_L204_L1.3200.91
366_L374_G1.3040.90
263_E341_Q1.3010.90
298_V307_V1.2870.89
181_L294_L1.2860.89
270_Y323_W1.2780.89
63_L72_I1.2740.89
236_T239_P1.2730.89
377_L380_A1.2700.89
191_L195_L1.2660.89
133_L171_L1.2610.89
266_Y283_L1.2600.88
32_L240_H1.2590.88
18_Y238_V1.2540.88
216_R220_L1.2480.88
3_L84_L1.2440.88
86_P236_T1.2380.88
24_N27_F1.2370.88
73_R283_L1.2350.87
42_Y48_T1.2310.87
235_F239_P1.2290.87
94_Y248_R1.2240.87
267_L348_K1.2190.87
88_E214_W1.2130.86
308_A361_F1.2010.86
40_Y95_F1.1940.86
228_F345_A1.1900.85
268_Y272_R1.1880.85
118_L132_F1.1810.85
125_Y293_L1.1800.85
22_V25_D1.1720.85
338_P362_S1.1720.85
115_A118_L1.1700.84
299_P304_K1.1640.84
18_Y235_F1.1590.84
74_T269_L1.1440.83
327_R342_R1.1410.83
21_Y25_D1.1360.83
18_Y24_N1.1350.83
45_W48_T1.1290.82
23_F36_G1.1230.82
186_L190_L1.1130.81
95_F212_L1.0990.80
221_E267_L1.0990.80
178_Y359_L1.0980.80
203_L355_L1.0950.80
71_P227_R1.0900.80
235_F286_R1.0890.80
108_L197_L1.0880.80
326_H335_Y1.0880.80
274_F353_L1.0870.80
15_Q21_Y1.0730.79
21_Y27_F1.0710.79
234_L313_Y1.0700.79
230_R302_W1.0690.79
26_H252_D1.0680.79
94_Y98_A1.0650.78
185_P191_L1.0610.78
43_S278_G1.0600.78
375_L378_L1.0580.78
193_L197_L1.0570.78
221_E298_V1.0570.78
323_W337_V1.0560.78
187_L190_L1.0550.78
42_Y119_P1.0540.78
310_L313_Y1.0490.77
30_V215_E1.0490.77
36_G39_A1.0420.77
339_E377_L1.0420.77
85_L238_V1.0390.77
36_G272_R1.0370.76
11_H15_Q1.0370.76
25_D33_F1.0350.76
39_A231_F1.0300.76
133_L186_L1.0300.76
167_L358_A1.0280.76
160_R163_R1.0270.76
113_V289_V1.0240.76
24_N232_A1.0210.75
83_F322_L1.0180.75
121_Y132_F1.0120.75
63_L113_V1.0110.75
314_L321_P1.0090.74
6_K285_L1.0060.74
219_E223_K1.0020.74
102_S147_L0.9960.73
110_L266_Y0.9910.73
62_V113_V0.9890.73
375_L379_L0.9850.73
23_F28_A0.9810.72
258_I267_L0.9730.72
235_F318_Q0.9670.71
75_L98_A0.9660.71
25_D153_E0.9650.71
223_K298_V0.9620.71
83_F101_Y0.9610.71
111_L116_V0.9600.71
75_L228_F0.9590.71
114_L132_F0.9540.70
48_T247_R0.9480.70
57_L60_A0.9450.69
170_F218_I0.9380.69
5_K9_R0.9370.69
29_L32_L0.9330.68
19_L30_V0.9300.68
284_Y357_Q0.9300.68
24_N255_L0.9240.68
85_L88_E0.9240.68
167_L178_Y0.9240.68
41_Y284_Y0.9230.68
3_L277_S0.9210.67
61_L355_L0.9200.67
141_L363_L0.9170.67
68_T72_I0.9130.67
53_F120_L0.9120.67
278_G332_L0.9100.67
301_P304_K0.9070.66
364_V368_S0.9060.66
39_A44_Q0.9050.66
79_A320_L0.9000.66
20_R247_R0.8980.65
236_T308_A0.8970.65
25_D272_R0.8960.65
159_K162_R0.8950.65
44_Q47_K0.8950.65
167_L285_L0.8930.65
154_L157_R0.8910.65
4_F11_H0.8880.65
266_Y335_Y0.8870.65
18_Y229_Y0.8860.64
30_V34_L0.8850.64
41_Y291_G0.8830.64
72_I106_Q0.8800.64
35_L294_L0.8760.64
86_P214_W0.8740.63
33_F111_L0.8740.63
233_N236_T0.8720.63
3_L180_L0.8680.63
224_R228_F0.8680.63
36_G73_R0.8660.63
31_L36_G0.8660.63
4_F91_M0.8640.62
292_A296_Y0.8640.62
82_V86_P0.8620.62
13_K17_K0.8590.62
319_L354_L0.8560.62
77_K363_L0.8520.61
45_W78_E0.8510.61
232_A236_T0.8510.61
25_D70_G0.8510.61
85_L221_E0.8500.61
20_R26_H0.8480.61
8_L12_W0.8460.61
56_P64_G0.8460.61
359_L366_L0.8450.61
186_L189_L0.8440.61
67_L156_L0.8440.61
4_F97_R0.8440.61
373_L377_L0.8430.61
310_L330_L0.8430.61
98_A160_R0.8420.60
233_N243_G0.8420.60
334_L342_R0.8410.60
166_R174_A0.8400.60
3_L7_R0.8390.60
11_H221_E0.8390.60
180_L290_I0.8370.60
54_P238_V0.8350.60
225_M273_T0.8330.60
4_F154_L0.8330.60
41_Y45_W0.8330.60
287_L311_F0.8320.60
330_L333_H0.8290.59
322_L325_H0.8260.59
81_L270_Y0.8260.59
25_D36_G0.8240.59
171_L198_A0.8240.59
46_L120_L0.8230.59
40_Y43_S0.8230.59
217_L232_A0.8140.58
261_K264_N0.8130.58
225_M229_Y0.8120.58
143_K270_Y0.8090.57
88_E266_Y0.8080.57
59_L75_L0.8060.57
83_F199_L0.8050.57
11_H277_S0.8050.57
151_W169_R0.8050.57
300_N303_L0.8050.57
18_Y25_D0.8000.57
370_G381_G0.8000.57
25_D37_A0.7990.56
6_K137_L0.7980.56
277_S325_H0.7940.56
94_Y279_D0.7930.56
36_G42_Y0.7930.56
8_L75_L0.7930.56
173_N185_P0.7920.56
22_V28_A0.7890.55
73_R153_E0.7880.55
122_V132_F0.7860.55
114_L118_L0.7800.55
270_Y332_L0.7790.55
99_L147_L0.7760.54
104_V167_L0.7750.54
121_Y127_L0.7700.54
18_Y313_Y0.7690.54
259_P265_T0.7690.54
248_R269_L0.7680.53
70_G277_S0.7680.53
159_K275_L0.7680.53
170_F178_Y0.7640.53
63_L216_R0.7630.53
106_Q280_Y0.7630.53
218_I251_L0.7620.53
184_A191_L0.7620.53
102_S234_L0.7590.53
112_L190_L0.7580.52
78_E304_K0.7570.52
37_A332_L0.7570.52
359_L378_L0.7530.52
353_L356_I0.7530.52
57_L209_K0.7510.52
264_N373_L0.7500.52
283_L321_P0.7490.52
230_R241_L0.7480.52
221_E269_L0.7460.51
338_P355_L0.7460.51
62_V375_L0.7460.51
11_H75_L0.7460.51
27_F105_L0.7460.51
14_E229_Y0.7410.51
54_P248_R0.7400.51
198_A343_K0.7400.51
128_S285_L0.7390.51
142_L177_L0.7390.51
120_L277_S0.7350.50
255_L268_Y0.7330.50
60_A196_L0.7320.50
279_D336_P0.7300.50
190_L359_L0.7300.50
6_K163_R0.7270.50
27_F323_W0.7270.50
235_F336_P0.7260.49
7_R80_D0.7230.49
12_W60_A0.7210.49
26_H272_R0.7210.49
82_V114_L0.7190.49
22_V257_L0.7180.49
338_P341_Q0.7170.49
316_G335_Y0.7160.48
146_N169_R0.7150.48
94_Y318_Q0.7130.48
36_G283_L0.7120.48
18_Y237_D0.7120.48
13_K225_M0.7120.48
45_W218_I0.7110.48
103_F106_Q0.7090.48
66_L306_I0.7080.48
26_H120_L0.7020.47
49_L305_L0.7020.47
311_F349_L0.7000.47
91_M114_L0.7000.47
14_E77_K0.6970.47
260_F268_Y0.6970.47
21_Y26_H0.6960.47
254_L258_I0.6950.46
126_G227_R0.6930.46
54_P232_A0.6930.46
104_V137_L0.6920.46
39_A43_S0.6920.46
284_Y326_H0.6900.46
4_F241_L0.6860.46
265_T268_Y0.6840.45
292_A356_I0.6840.45
201_L261_K0.6840.45
27_F31_L0.6840.45
25_D224_R0.6810.45
274_F284_Y0.6800.45
15_Q26_H0.6780.45
328_Y333_H0.6780.45
106_Q220_L0.6770.45
29_L319_L0.6760.45
15_Q25_D0.6750.45
92_K103_F0.6750.45
17_K24_N0.6750.45
314_L346_F0.6740.44
85_L236_T0.6730.44
254_L355_L0.6710.44
273_T322_L0.6700.44
372_L376_L0.6690.44
167_L363_L0.6660.44
3_L21_Y0.6650.44
126_G190_L0.6650.44
66_L194_L0.6640.43
6_K189_L0.6610.43
64_G232_A0.6600.43
74_T237_D0.6600.43
19_L232_A0.6590.43
244_R278_G0.6580.43
7_R276_R0.6570.43
22_V95_F0.6560.43
113_V340_E0.6550.43
245_V293_L0.6550.43
18_Y26_H0.6550.43
15_Q32_L0.6540.43
268_Y318_Q0.6520.42
274_F287_L0.6510.42
55_A124_V0.6490.42
21_Y30_V0.6490.42
47_K367_I0.6480.42
37_A41_Y0.6480.42
90_K366_L0.6450.42
63_L139_L0.6450.42
344_K348_K0.6450.42
245_V282_G0.6440.42
37_A98_A0.6440.42
218_I377_L0.6440.42
37_A314_L0.6430.41
76_L95_F0.6410.41
214_W270_Y0.6400.41
271_L312_L0.6390.41
135_L244_R0.6390.41
294_L301_P0.6370.41
279_D286_R0.6370.41
251_L281_L0.6360.41
159_K195_L0.6300.40
10_A104_V0.6300.40
91_M360_L0.6290.40
287_L293_L0.6290.40
143_K335_Y0.6280.40
38_G188_A0.6250.40
50_P55_A0.6250.40
151_W223_K0.6240.40
17_K224_R0.6230.40
143_K282_G0.6220.40
13_K16_L0.6210.39
107_L344_K0.6190.39
67_L201_L0.6190.39
377_L381_G0.6190.39
41_Y44_Q0.6170.39
19_L32_L0.6170.39
171_L261_K0.6150.39
50_P101_Y0.6130.39
253_W275_L0.6110.39
59_L180_L0.6110.39
228_F315_T0.6110.39
68_T147_L0.6100.38
24_N29_L0.6080.38
260_F331_W0.6070.38
371_L379_L0.6060.38
40_Y309_L0.6050.38
79_A334_L0.6040.38
224_R227_R0.6040.38
134_L250_W0.5990.37
25_D252_D0.5980.37
218_I362_S0.5980.37
110_L360_L0.5970.37
24_N318_Q0.5940.37
338_P366_L0.5940.37
16_L20_R0.5930.37
98_A343_K0.5910.37
116_V222_Q0.5910.37
164_L289_V0.5900.37
64_G244_R0.5890.37
18_Y27_F0.5880.36
22_V69_Y0.5870.36
341_Q345_A0.5860.36
135_L262_Q0.5830.36
131_S135_L0.5820.36
93_G358_A0.5810.36
83_F211_G0.5760.35
79_A361_F0.5760.35
61_L174_A0.5740.35
350_L361_F0.5740.35
41_Y245_V0.5740.35
14_E25_D0.5730.35
12_W101_Y0.5710.35
246_K249_K0.5700.35
12_W225_M0.5700.35
222_Q356_I0.5680.35
15_Q30_V0.5670.35
71_P272_R0.5660.34
86_P272_R0.5650.34
267_L345_A0.5630.34
36_G252_D0.5630.34
121_Y266_Y0.5620.34
63_L225_M0.5610.34
352_R362_S0.5610.34
68_T117_L0.5590.34
103_F226_M0.5590.34
120_L129_F0.5570.34
23_F81_L0.5570.34
107_L112_L0.5560.34
24_N236_T0.5560.34
46_L361_F0.5560.34
141_L198_A0.5560.34
173_N289_V0.5550.34
92_K191_L0.5540.33
143_K147_L0.5530.33
44_Q247_R0.5520.33
30_V272_R0.5520.33
26_H29_L0.5510.33
53_F74_T0.5500.33
150_Q227_R0.5480.33
94_Y237_D0.5480.33
10_A77_K0.5480.33
200_L293_L0.5480.33
31_L218_I0.5470.33
109_L143_K0.5460.33
156_L207_R0.5450.33
180_L185_P0.5450.33
24_N36_G0.5450.33
118_L122_V0.5440.33
339_E375_L0.5440.33
40_Y44_Q0.5430.33
81_L218_I0.5420.32
136_L262_Q0.5420.32
32_L300_N0.5420.32
178_Y184_A0.5410.32
89_K177_L0.5400.32
55_A232_A0.5380.32
241_L315_T0.5380.32
303_L327_R0.5370.32
34_L304_K0.5370.32
8_L11_H0.5370.32
209_K259_P0.5360.32
15_Q277_S0.5350.32
75_L166_R0.5350.32
37_A40_Y0.5350.32
175_L182_S0.5330.32
193_L198_A0.5310.32
18_Y286_R0.5300.31
58_I258_I0.5290.31
72_I102_S0.5290.31
235_F282_G0.5280.31
67_L240_H0.5280.31
159_K356_I0.5260.31
235_F268_Y0.5260.31
94_Y269_L0.5250.31
88_E336_P0.5250.31
106_Q331_W0.5230.31
96_K199_L0.5230.31
179_L189_L0.5220.31
100_R104_V0.5210.31
164_L376_L0.5190.31
312_L354_L0.5180.30
39_A284_Y0.5170.30
79_A374_G0.5170.30
126_G340_E0.5160.30
107_L198_A0.5130.30
125_Y135_L0.5120.30
172_L347_L0.5120.30
98_A303_L0.5120.30
137_L140_L0.5100.30
111_L306_I0.5100.30
26_H45_W0.5100.30
191_L227_R0.5090.30
20_R230_R0.5090.30
11_H14_E0.5090.30
308_A347_L0.5080.30
49_L312_L0.5070.30
189_L306_I0.5060.29
27_F310_L0.5040.29
89_K275_L0.5040.29
226_M264_N0.5030.29
43_S46_L0.5020.29
113_V133_L0.5010.29
104_V256_R0.5000.29
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4aq5C 1 0.1358 14.2 0.965 Contact Map
3w4tA 1 0.5379 9.3 0.967 Contact Map
3giaA 1 0.5379 5.8 0.97 Contact Map
4aq5E 1 0.1332 5.8 0.97 Contact Map
3l1lA 2 0.5405 5.7 0.97 Contact Map
3mktA 1 0.5666 3.8 0.973 Contact Map
4humA 1 0.5692 3.1 0.974 Contact Map
2kogA 1 0.1044 3 0.974 Contact Map
4lz6A 1 0.5483 2.5 0.975 Contact Map
4tq3A 1 0.2924 2.2 0.976 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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