GREMLIN Database
T6SS_VasE - Bacterial Type VI secretion, VC_A0110, EvfL, ImpJ, VasE
PFAM: PF05936 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 427 (426)
Sequences: 4523 (3168)
Seq/√Len: 153.5
META: 0.725

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
278_D281_A5.9581.00
29_E46_R4.7721.00
154_R166_D3.8391.00
337_D419_G3.7421.00
305_I317_A3.5911.00
49_G57_F3.5791.00
85_A138_L3.4851.00
193_L226_L3.3981.00
59_I65_L3.3241.00
232_L253_R3.2671.00
81_T151_P3.2481.00
275_D278_D3.2411.00
151_P169_F3.2411.00
246_R249_R3.0461.00
32_I42_L2.9891.00
31_E46_R2.9561.00
250_E253_R2.8071.00
31_E45_R2.6451.00
81_T154_R2.6151.00
82_V139_L2.5931.00
279_L282_C2.5801.00
83_Y149_T2.5531.00
347_F351_V2.5531.00
81_T140_E2.4841.00
228_R257_E2.4291.00
27_F47_A2.3851.00
334_V347_F2.3701.00
29_E48_S2.3571.00
233_L251_L2.3351.00
235_H245_E2.3111.00
58_D61_G2.2951.00
333_A388_Y2.2921.00
51_F135_L2.2671.00
55_T86_L2.2281.00
42_L153_A2.2261.00
112_E127_P2.2211.00
111_E132_R2.2201.00
271_L289_L2.1241.00
34_E167_P2.1101.00
185_L287_D2.0751.00
197_A226_L2.0551.00
151_P154_R2.0151.00
358_R402_Q2.0031.00
193_L254_L2.0001.00
30_L44_L1.9931.00
355_S399_L1.9851.00
231_P256_G1.9801.00
156_V166_D1.9791.00
69_L142_E1.9721.00
319_L325_L1.9131.00
338_M343_L1.9061.00
114_E125_E1.9021.00
409_I424_L1.8941.00
5_Q9_L1.8891.00
307_L317_A1.8771.00
186_L247_L1.8701.00
144_R149_T1.8631.00
309_E315_Y1.8441.00
47_A57_F1.8411.00
80_L141_G1.8351.00
42_L137_L1.8311.00
153_A165_L1.8191.00
342_E346_R1.7911.00
33_D45_R1.7761.00
331_V375_L1.7661.00
59_I135_L1.7661.00
115_V130_V1.7641.00
52_P148_V1.7451.00
48_S58_D1.7341.00
33_D43_A1.7241.00
394_D397_G1.7221.00
88_L148_V1.7161.00
109_R134_N1.7131.00
193_L233_L1.7121.00
370_I393_L1.6911.00
370_I403_L1.6881.00
9_L13_L1.6881.00
113_R130_V1.6751.00
262_S293_L1.6711.00
353_I411_L1.6671.00
252_L286_L1.6641.00
27_F152_I1.6501.00
138_L147_Y1.6381.00
170_I179_S1.6081.00
169_F172_P1.6051.00
85_A149_T1.5811.00
115_V128_V1.5711.00
198_E267_R1.5641.00
47_A59_I1.5631.00
6_D9_L1.5351.00
170_I279_L1.5221.00
289_L292_R1.5221.00
154_R280_G1.5211.00
317_A409_I1.5171.00
335_R421_E1.5151.00
229_Y254_L1.5151.00
85_A147_Y1.4981.00
329_R392_E1.4941.00
280_G284_A1.4781.00
47_A84_L1.4771.00
36_A43_A1.4701.00
192_L291_R1.4661.00
41_K70_D1.4631.00
114_E127_P1.4551.00
138_L144_R1.4551.00
176_I186_L1.4361.00
229_Y250_E1.4351.00
197_A230_E1.4351.00
8_Y12_L1.4261.00
97_A106_A1.4241.00
371_P397_G1.4181.00
194_R230_E1.4171.00
255_A271_L1.4161.00
397_G401_E1.4111.00
361_E365_L1.4031.00
353_I393_L1.3981.00
14_E18_R1.3941.00
236_L250_E1.3921.00
284_A288_A1.3901.00
53_D148_V1.3881.00
31_E34_E1.3841.00
232_L250_E1.3591.00
168_D284_A1.3581.00
83_Y140_E1.3571.00
334_V422_L1.3561.00
170_I280_G1.3521.00
403_L409_I1.3481.00
28_S48_S1.3361.00
110_A129_Q1.3141.00
401_E404_L1.3121.00
335_R388_Y1.3101.00
358_R361_E1.3081.00
344_R374_H1.3031.00
27_F30_L1.3011.00
326_R404_L1.2991.00
27_F150_L1.2741.00
371_P394_D1.2691.00
169_F279_L1.2651.00
271_L286_L1.2631.00
156_V164_V1.2551.00
354_G362_L1.2501.00
182_L283_F1.2391.00
25_W277_D1.2371.00
373_R392_E1.2361.00
248_Y282_C1.2361.00
42_L82_V1.2341.00
21_S246_R1.2271.00
352_K367_L1.2261.00
356_V408_G1.2181.00
289_L293_L1.2151.00
74_P77_A1.1981.00
353_I372_L1.1961.00
310_R316_V1.1891.00
170_I182_L1.1771.00
245_E249_R1.1731.00
248_Y273_A1.1671.00
190_L234_R1.1651.00
90_R93_G1.1601.00
79_D154_R1.1581.00
89_R95_N1.1561.00
49_G150_L1.1561.00
80_L139_L1.1541.00
189_L287_D1.1491.00
32_I165_L1.1461.00
121_T124_D1.1421.00
69_L139_L1.1361.00
227_N260_T1.1331.00
247_L251_L1.1311.00
243_H274_Y1.1251.00
88_L146_G1.1191.00
264_E292_R1.1191.00
32_I44_L1.1191.00
334_V343_L1.1161.00
357_E406_S1.1150.99
188_E287_D1.1000.99
170_I181_R1.0980.99
288_A292_R1.0930.99
86_L133_L1.0880.99
46_R61_G1.0870.99
72_P77_A1.0860.99
238_E273_A1.0740.99
402_Q405_R1.0730.99
42_L165_L1.0700.99
230_E267_R1.0700.99
281_A285_P1.0650.99
86_L135_L1.0610.99
399_L402_Q1.0600.99
63_D133_L1.0580.99
308_E318_R1.0540.99
173_C179_S1.0540.99
112_E129_Q1.0540.99
255_A286_L1.0350.99
49_G84_L1.0340.99
189_L233_L1.0330.99
82_V137_L1.0280.99
27_F49_G1.0280.99
336_A343_L1.0260.99
252_L271_L1.0260.99
85_A136_R1.0250.99
70_D73_E1.0240.99
324_L328_A1.0240.99
141_G144_R1.0210.99
223_L227_N1.0160.99
413_V422_L1.0100.99
374_H389_Q1.0080.99
68_P88_L1.0020.99
173_C178_A1.0000.99
69_L82_V0.9980.99
55_T133_L0.9920.99
181_R284_A0.9910.99
248_Y252_L0.9900.99
143_D149_T0.9900.99
351_V372_L0.9870.99
356_V359_I0.9840.99
60_P63_D0.9790.99
385_R388_Y0.9790.99
44_L59_I0.9780.99
225_T257_E0.9780.99
139_L142_E0.9760.99
71_L155_V0.9750.99
309_E312_Y0.9750.99
411_L424_L0.9700.99
285_P288_A0.9660.99
339_P342_E0.9640.99
353_I409_I0.9610.98
57_F135_L0.9590.98
239_A242_L0.9570.98
96_V110_A0.9560.98
360_R364_N0.9470.98
230_E234_R0.9430.98
358_R399_L0.9330.98
69_L137_L0.9320.98
155_V165_L0.9320.98
334_V420_L0.9320.98
83_Y144_R0.9240.98
187_R237_L0.9240.98
188_E291_R0.9230.98
357_E408_G0.9180.98
243_H276_H0.9170.98
359_I410_A0.9160.98
173_C279_L0.9150.98
13_L16_R0.9120.98
23_Y239_A0.9110.98
32_I153_A0.9100.98
287_D291_R0.9020.98
354_G358_R0.8990.98
331_V427_I0.8970.98
7_R118_E0.8930.98
395_R400_W0.8900.98
19_A28_S0.8840.97
275_D281_A0.8840.97
27_F173_C0.8830.97
172_P279_L0.8810.97
96_V119_N0.8780.97
194_R234_R0.8770.97
38_A162_Q0.8770.97
225_T254_L0.8770.97
214_A217_V0.8760.97
354_G364_N0.8720.97
80_L140_E0.8710.97
44_L137_L0.8710.97
25_W150_L0.8690.97
321_D325_L0.8680.97
136_R147_Y0.8680.97
272_P285_P0.8680.97
50_V56_P0.8630.97
11_A15_F0.8590.97
159_R162_Q0.8540.97
60_P66_P0.8530.97
302_A383_P0.8530.97
316_V356_V0.8520.97
121_T125_E0.8510.97
362_L369_G0.8510.97
356_V410_A0.8490.97
82_V155_V0.8480.97
352_K366_A0.8430.97
359_I363_V0.8410.97
42_L71_L0.8410.97
137_L152_I0.8410.97
96_V131_A0.8370.96
341_E345_R0.8300.96
53_D107_R0.8300.96
356_V360_R0.8290.96
83_Y151_P0.8250.96
234_R238_E0.8220.96
248_Y271_L0.8210.96
99_E102_A0.8200.96
373_R394_D0.8200.96
6_D10_E0.8180.96
81_T169_F0.8150.96
16_R50_V0.8150.96
64_P132_R0.8140.96
358_R365_L0.8140.96
173_C176_I0.8140.96
244_P274_Y0.8130.96
77_A139_L0.8110.96
375_L378_P0.8100.96
27_F57_F0.8090.96
221_L224_Q0.8010.95
197_A267_R0.8000.95
224_Q257_E0.7990.95
302_A425_W0.7960.95
102_A109_R0.7960.95
323_R326_R0.7960.95
295_S299_E0.7950.95
79_D140_E0.7940.95
255_A289_L0.7940.95
346_R349_A0.7940.95
65_L135_L0.7900.95
340_A344_R0.7880.95
193_L197_A0.7840.95
229_Y253_R0.7840.95
338_M417_F0.7830.95
111_E130_V0.7830.95
30_L152_I0.7820.95
183_A237_L0.7790.95
316_V408_G0.7770.95
44_L65_L0.7750.95
12_L56_P0.7750.95
197_A227_N0.7740.94
387_G392_E0.7730.94
282_C285_P0.7730.94
49_G135_L0.7700.94
388_Y421_E0.7680.94
374_H378_P0.7680.94
223_L260_T0.7630.94
58_D62_D0.7620.94
307_L411_L0.7620.94
158_D164_V0.7610.94
4_Q7_R0.7600.94
357_E364_N0.7590.94
285_P289_L0.7560.94
329_R381_Q0.7520.94
179_S182_L0.7510.94
249_R252_L0.7500.94
175_D241_G0.7480.93
357_E402_Q0.7450.93
192_L196_R0.7450.93
232_L249_R0.7440.93
23_Y245_E0.7400.93
169_F277_D0.7380.93
304_S423_E0.7360.93
19_A56_P0.7350.93
332_L393_L0.7340.93
67_P70_D0.7330.93
398_P401_E0.7330.93
220_F363_V0.7310.93
105_L112_E0.7300.93
28_S174_L0.7290.93
83_Y143_D0.7270.92
415_G418_P0.7250.92
255_A259_A0.7250.92
281_A284_A0.7240.92
51_F57_F0.7180.92
379_P390_Y0.7170.92
413_V417_F0.7160.92
18_R21_S0.7160.92
177_S241_G0.7100.92
377_V380_R0.7080.91
35_E45_R0.7070.91
238_E275_D0.7060.91
172_P179_S0.7040.91
94_A119_N0.7020.91
109_R132_R0.7010.91
408_G411_L0.7000.91
170_I173_C0.6980.91
357_E361_E0.6980.91
380_R384_Y0.6960.91
372_L413_V0.6950.91
259_A293_L0.6930.91
158_D161_G0.6930.91
236_L242_L0.6930.91
67_P71_L0.6920.90
190_L233_L0.6860.90
222_L297_V0.6830.90
331_V382_I0.6820.90
344_R348_P0.6760.89
60_P65_L0.6750.89
216_D220_F0.6750.89
173_C182_L0.6750.89
272_P282_C0.6750.89
9_L12_L0.6720.89
71_L163_V0.6720.89
140_E151_P0.6700.89
174_L279_L0.6670.89
138_L142_E0.6670.89
393_L424_L0.6650.89
361_E364_N0.6640.89
275_D282_C0.6630.89
176_I183_A0.6620.88
49_G59_I0.6620.88
183_A187_R0.6610.88
342_E345_R0.6610.88
223_L324_L0.6590.88
322_R326_R0.6570.88
35_E39_N0.6560.88
244_P276_H0.6530.88
202_R212_F0.6520.88
217_V221_L0.6510.88
338_M342_E0.6480.87
47_A152_I0.6480.87
16_R56_P0.6480.87
155_V163_V0.6480.87
231_P250_E0.6480.87
341_E344_R0.6470.87
4_Q115_V0.6460.87
179_S183_A0.6460.87
399_L403_L0.6440.87
43_A68_P0.6440.87
252_L282_C0.6430.87
398_P402_Q0.6430.87
175_D236_L0.6410.87
5_Q8_Y0.6410.87
377_V384_Y0.6390.87
44_L47_A0.6370.86
255_A290_L0.6370.86
31_E152_I0.6370.86
176_I247_L0.6360.86
215_A225_T0.6350.86
136_R142_E0.6350.86
207_P214_A0.6330.86
418_P422_L0.6310.86
37_L163_V0.6290.86
177_S238_E0.6280.86
51_F148_V0.6280.86
403_L408_G0.6270.86
87_P138_L0.6270.86
143_D147_Y0.6270.86
120_S125_E0.6260.86
153_A258_L0.6250.85
218_A296_T0.6240.85
189_L251_L0.6240.85
204_L211_G0.6230.85
3_Q6_D0.6200.85
120_S123_A0.6190.85
84_L135_L0.6190.85
246_R250_E0.6180.85
346_R350_Q0.6180.85
354_G399_L0.6180.85
321_D324_L0.6170.85
160_D184_G0.6170.85
324_L426_A0.6170.85
95_N118_E0.6150.85
407_G414_P0.6140.84
196_R301_R0.6140.84
79_D157_R0.6140.84
345_R349_A0.6130.84
79_D156_V0.6130.84
57_F133_L0.6130.84
86_L148_V0.6110.84
222_L366_A0.6110.84
186_L190_L0.6100.84
401_E405_R0.6100.84
138_L149_T0.6080.84
269_P289_L0.6080.84
137_L165_L0.6080.84
400_W403_L0.6070.84
228_R253_R0.6070.84
227_N261_F0.6050.84
17_A20_L0.6040.83
229_Y270_D0.6040.83
352_K359_I0.6040.83
355_S358_R0.6040.83
238_E252_L0.6030.83
297_V300_Q0.6030.83
381_Q427_I0.6020.83
262_S296_T0.6000.83
21_S162_Q0.5990.83
84_L87_P0.5990.83
411_L420_L0.5990.83
370_I395_R0.5990.83
7_R53_D0.5980.83
69_L73_E0.5980.83
335_R387_G0.5980.83
78_R195_A0.5980.83
110_A259_A0.5960.83
44_L152_I0.5930.82
119_N122_G0.5920.82
313_G363_V0.5910.82
177_S183_A0.5900.82
185_L189_L0.5900.82
175_D240_P0.5890.82
314_I359_I0.5890.82
30_L47_A0.5880.82
64_P67_P0.5850.81
2_F119_N0.5850.81
113_R241_G0.5850.81
322_R407_G0.5850.81
39_N191_G0.5830.81
190_L230_E0.5830.81
257_E260_T0.5830.81
150_L172_P0.5820.81
360_R363_V0.5800.81
393_L403_L0.5800.81
313_G360_R0.5790.81
55_T107_R0.5790.81
21_S24_P0.5780.81
278_D282_C0.5770.81
77_A80_L0.5750.80
186_L292_R0.5750.80
101_A104_S0.5750.80
363_V366_A0.5740.80
312_Y315_Y0.5740.80
139_L153_A0.5730.80
151_P277_D0.5710.80
63_D66_P0.5700.80
211_G215_A0.5690.80
236_L240_P0.5680.80
120_S124_D0.5680.80
199_A300_Q0.5660.79
51_F150_L0.5660.79
67_P73_E0.5660.79
325_L330_F0.5660.79
15_F58_D0.5650.79
316_V410_A0.5650.79
13_L17_A0.5650.79
42_L51_F0.5640.79
382_I425_W0.5640.79
279_L284_A0.5640.79
197_A313_G0.5630.79
156_V168_D0.5620.79
308_E316_V0.5620.79
145_D215_A0.5620.79
201_A227_N0.5620.79
338_M346_R0.5610.79
176_I242_L0.5610.79
84_L152_I0.5610.79
207_P213_G0.5600.79
213_G216_D0.5580.78
15_F56_P0.5580.78
312_Y364_N0.5570.78
206_G220_F0.5570.78
27_F169_F0.5570.78
46_R167_P0.5560.78
138_L304_S0.5540.78
73_E77_A0.5530.78
355_S403_L0.5530.78
381_Q384_Y0.5520.78
310_R318_R0.5520.78
125_E233_L0.5510.77
203_R215_A0.5490.77
344_R354_G0.5490.77
412_H420_L0.5470.77
171_P279_L0.5460.77
171_P178_A0.5450.77
204_L207_P0.5450.77
220_F245_E0.5450.77
224_Q261_F0.5430.76
222_L226_L0.5430.76
84_L150_L0.5420.76
127_P337_D0.5410.76
231_P268_P0.5390.76
210_A214_A0.5380.76
175_D239_A0.5380.76
139_L155_V0.5380.76
151_P172_P0.5370.76
57_F84_L0.5370.76
128_V361_E0.5360.76
105_L155_V0.5360.76
314_I364_N0.5350.75
405_R409_I0.5350.75
347_F413_V0.5340.75
372_L393_L0.5340.75
2_F5_Q0.5330.75
259_A267_R0.5330.75
122_G125_E0.5320.75
117_D124_D0.5320.75
16_R19_A0.5320.75
4_Q128_V0.5320.75
1_H26_G0.5310.75
242_L246_R0.5300.75
305_I409_I0.5300.75
186_L239_A0.5280.74
328_A426_A0.5280.74
191_G195_A0.5280.74
311_K408_G0.5260.74
159_R164_V0.5250.74
167_P184_G0.5250.74
146_G275_D0.5240.74
220_F227_N0.5230.74
262_S298_L0.5230.74
115_V127_P0.5230.74
386_A389_Q0.5230.74
10_E133_L0.5230.74
14_E146_G0.5220.74
393_L396_S0.5220.74
362_L375_L0.5220.74
36_A41_K0.5210.74
220_F261_F0.5210.74
230_E400_W0.5210.74
374_H391_F0.5210.74
216_D222_L0.5200.73
123_A147_Y0.5200.73
185_L233_L0.5200.73
324_L330_F0.5200.73
395_R404_L0.5170.73
220_F225_T0.5170.73
214_A218_A0.5170.73
59_I382_I0.5170.73
205_R216_D0.5170.73
49_G131_A0.5170.73
194_R198_E0.5160.73
419_G425_W0.5160.73
109_R168_D0.5160.73
354_G359_I0.5160.73
278_D284_A0.5160.73
413_V420_L0.5150.73
71_L139_L0.5150.73
353_I403_L0.5130.72
343_L347_F0.5120.72
407_G420_L0.5120.72
271_L282_C0.5120.72
8_Y11_A0.5110.72
29_E45_R0.5110.72
2_F148_V0.5100.72
261_F351_V0.5090.72
1_H6_D0.5090.72
58_D411_L0.5080.72
10_E20_L0.5080.72
28_S175_D0.5080.72
117_D379_P0.5070.72
224_Q260_T0.5070.72
91_P162_Q0.5060.71
259_A289_L0.5060.71
383_P390_Y0.5060.71
6_D13_L0.5060.71
252_L416_E0.5050.71
14_E56_P0.5050.71
388_Y423_E0.5040.71
370_I399_L0.5040.71
153_A169_F0.5040.71
118_E131_A0.5020.71
344_R389_Q0.5020.71
211_G214_A0.5020.71
352_K365_L0.5010.71
89_R118_E0.5010.71
380_R427_I0.5000.70
370_I397_G0.5000.70
74_P80_L0.5000.70
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3lpeA 1 0.1944 12.3 0.974 Contact Map
2l3lA 1 0.26 7.6 0.976 Contact Map
2re7A 2 0.1944 4.3 0.979 Contact Map
1o9aB 1 0.0562 4.2 0.979 Contact Map
3dmbA 5 0.1967 4.1 0.979 Contact Map
3ec6A 2 0.1827 3.9 0.979 Contact Map
2nr4A 2 0.1733 3.5 0.979 Contact Map
2i02A 2 0.1874 3.5 0.979 Contact Map
2ol5A 2 0.185 3.4 0.98 Contact Map
2fhqA 2 0.192 3.4 0.98 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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