GREMLIN Database
Fumerase - Fumarate hydratase (Fumerase)
PFAM: PF05681 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 269 (268)
Sequences: 14182 (6657)
Seq/√Len: 406.6
META: 0.877

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
159_K217_E3.1321.00
5_A94_E2.9911.00
68_K130_K2.9761.00
27_L40_A2.8671.00
5_A90_R2.7441.00
164_E168_T2.5031.00
31_A235_K2.4221.00
122_E196_K2.3451.00
159_K221_K2.3281.00
20_L50_A2.2771.00
90_R94_E2.2661.00
89_R113_D2.2381.00
68_K122_E2.2071.00
62_T101_V2.1821.00
81_E122_E2.1531.00
47_E51_E2.1231.00
145_A168_T2.1091.00
132_T268_E1.9751.00
260_S264_D1.9701.00
79_D82_E1.9681.00
85_N117_A1.9641.00
25_K29_D1.9641.00
86_E89_R1.9571.00
10_D14_Y1.9361.00
130_K268_E1.9291.00
150_N153_D1.9051.00
81_E85_N1.8891.00
14_Y50_A1.8561.00
44_E47_E1.8371.00
187_S191_A1.8271.00
220_E224_A1.8101.00
56_I263_A1.7991.00
107_T210_E1.7821.00
67_V131_I1.7741.00
147_Y161_F1.7541.00
138_G252_S1.7541.00
75_I129_L1.7491.00
145_A165_T1.7131.00
205_R216_E1.7031.00
3_A269_A1.6951.00
200_R244_E1.6511.00
189_K246_L1.6491.00
146_L187_S1.6451.00
160_D164_E1.6441.00
222_I238_A1.6401.00
65_V133_V1.6391.00
81_E121_I1.6201.00
173_A177_G1.6161.00
219_L238_A1.5991.00
21_A25_K1.5951.00
17_P21_A1.5951.00
40_A44_E1.5741.00
205_R240_D1.5691.00
6_E10_D1.5661.00
86_E90_R1.5651.00
177_G238_A1.5601.00
167_K225_L1.5591.00
32_S35_A1.5051.00
206_N211_L1.4971.00
156_E160_D1.4851.00
143_K172_N1.4831.00
211_L245_R1.4701.00
103_A111_T1.4621.00
8_C91_A1.4531.00
101_V111_T1.4491.00
20_L24_A1.4391.00
67_V80_L1.4341.00
5_A9_I1.4281.00
11_A267_A1.4201.00
14_Y53_K1.4191.00
35_A229_P1.4131.00
155_L159_K1.4091.00
88_V117_A1.4001.00
8_C133_V1.3931.00
13_Y53_K1.3901.00
65_V84_I1.3511.00
155_L217_E1.3501.00
194_A258_N1.3411.00
201_P204_T1.3381.00
22_A25_K1.3221.00
212_A216_E1.3211.00
24_A43_L1.3201.00
196_K244_E1.3131.00
162_V218_L1.3031.00
179_L222_I1.3011.00
9_I95_G1.2971.00
204_T242_K1.2901.00
103_A109_K1.2841.00
62_T138_G1.2801.00
65_V88_V1.2631.00
9_I13_Y1.2611.00
162_V181_V1.2601.00
10_D267_A1.2561.00
79_D125_P1.2501.00
154_G157_G1.2501.00
163_L221_K1.2501.00
185_G189_K1.2441.00
67_V84_I1.2411.00
93_T99_K1.2411.00
181_V257_V1.2351.00
155_L214_L1.2321.00
73_V129_L1.2211.00
120_H192_L1.2161.00
160_D221_K1.2151.00
20_L47_E1.2081.00
8_C63_A1.2011.00
138_G248_T1.1881.00
67_V121_I1.1631.00
104_D245_R1.1571.00
11_A135_P1.1531.00
109_K112_G1.1481.00
142_N252_S1.1471.00
103_A107_T1.1471.00
3_A6_E1.1431.00
177_G259_V1.1371.00
203_G240_D1.1281.00
203_G242_K1.1281.00
148_M255_V1.1141.00
179_L259_V1.1091.00
24_A28_E1.1081.00
220_E223_N1.1061.00
48_I52_G1.1051.00
27_L36_K1.1051.00
210_E213_E1.1041.00
165_T168_T1.1011.00
166_V222_I1.0981.00
2_E90_R1.0951.00
221_K224_A1.0941.00
38_V232_L1.0881.00
71_Q125_P1.0821.00
24_A47_E1.0791.00
211_L243_I1.0771.00
68_K196_K1.0711.00
20_L43_L1.0641.00
20_L46_A1.0631.00
149_L157_G1.0531.00
81_E119_I1.0521.00
146_L191_A1.0491.00
84_I119_I1.0491.00
217_E221_K1.0461.00
4_V84_I1.0441.00
214_L243_I1.0441.00
157_G160_D1.0401.00
208_D245_R1.0381.00
144_S187_S1.0351.00
63_A133_V1.0341.00
248_T252_S1.0291.00
216_E220_E1.0281.00
21_A24_A1.0251.00
22_A26_A1.0241.00
22_A202_L1.0181.00
204_T207_P1.0151.00
7_L267_A1.0141.00
43_L46_A1.0051.00
2_E6_E1.0031.00
69_I121_I1.0031.00
192_L196_K1.0001.00
107_T208_D0.9961.00
92_Y99_K0.9911.00
188_E192_L0.9891.00
197_A244_E0.9841.00
104_D107_T0.9821.00
221_K225_L0.9801.00
104_D210_E0.9801.00
213_E217_E0.9711.00
147_Y164_E0.9691.00
163_L218_L0.9651.00
29_D235_K0.9611.00
37_D40_A0.9491.00
191_A256_A0.9441.00
187_S256_A0.9411.00
29_D236_T0.9401.00
15_L46_A0.9331.00
161_F165_T0.9271.00
27_L43_L0.9211.00
71_Q123_I0.9181.00
189_K192_L0.9121.00
104_D211_L0.9081.00
132_T199_L0.9051.00
69_I129_L0.8991.00
25_K28_E0.8971.00
12_S135_P0.8941.00
45_N49_A0.8851.00
165_T169_A0.8841.00
186_T190_A0.8811.00
163_L167_K0.8781.00
89_R93_T0.8741.00
219_L241_V0.8721.00
145_A161_F0.8701.00
218_L222_I0.8661.00
164_E167_K0.8651.00
182_G193_L0.8641.00
23_L39_L0.8611.00
149_L153_D0.8601.00
6_E9_I0.8561.00
83_A87_G0.8561.00
193_L244_E0.8561.00
161_F255_V0.8551.00
202_L240_D0.8521.00
159_K163_L0.8491.00
85_N119_I0.8431.00
193_L246_L0.8421.00
82_E85_N0.8401.00
37_D41_Q0.8371.00
108_R248_T0.8331.00
178_I202_L0.8251.00
229_P236_T0.8221.00
69_I123_I0.8041.00
66_F192_L0.7981.00
8_C12_S0.7961.00
120_H188_E0.7901.00
189_K196_K0.7891.00
7_L269_A0.7881.00
153_D160_D0.7881.00
166_V179_L0.7871.00
204_T212_A0.7861.00
35_A236_T0.7841.00
209_P213_E0.7831.00
150_N157_G0.7831.00
19_V264_D0.7821.00
91_A95_G0.7811.00
144_S191_A0.7811.00
76_T79_D0.7811.00
156_E159_K0.7791.00
183_I243_I0.7761.00
206_N212_A0.7751.00
158_V183_I0.7741.00
15_L56_I0.7741.00
26_A39_L0.7691.00
143_K173_A0.7671.00
5_A91_A0.7671.00
155_L213_E0.7671.00
159_K214_L0.7651.00
213_E216_E0.7581.00
180_G197_A0.7551.00
87_G91_A0.7551.00
171_G230_Q0.7551.00
66_F199_L0.7511.00
195_K258_N0.7501.00
205_R212_A0.7491.00
101_V247_P0.7491.00
200_R203_G0.7481.00
76_T80_L0.7441.00
143_K169_A0.7431.00
163_L225_L0.7371.00
9_I53_K0.7351.00
205_R242_K0.7351.00
9_I91_A0.7341.00
159_K218_L0.7331.00
62_T118_V0.7331.00
190_A256_A0.7261.00
8_C87_G0.7261.00
68_K124_V0.7251.00
153_D157_G0.7241.00
12_S97_L0.7241.00
23_L42_I0.7231.00
68_K132_T0.7221.00
44_E48_I0.7211.00
203_G206_N0.7191.00
72_D127_D0.7181.00
24_A27_L0.7091.00
7_L133_V0.7081.00
155_L210_E0.7071.00
206_N209_P0.7051.00
207_P210_E0.7051.00
187_S190_A0.7031.00
34_N38_V0.7031.00
8_C65_V0.7031.00
211_L214_L0.7011.00
166_V259_V0.6971.00
193_L196_K0.6961.00
13_Y54_L0.6831.00
106_L183_I0.6831.00
26_A236_T0.6831.00
16_P19_V0.6811.00
18_D202_L0.6801.00
80_L121_I0.6770.99
208_D211_L0.6770.99
36_K40_A0.6760.99
107_T211_L0.6750.99
146_L256_A0.6750.99
169_A173_A0.6740.99
3_A7_L0.6740.99
14_Y20_L0.6680.99
200_R206_N0.6670.99
200_R204_T0.6670.99
178_I264_D0.6550.99
145_A164_E0.6550.99
63_A92_Y0.6550.99
144_S258_N0.6540.99
72_D126_G0.6530.99
64_V258_N0.6510.99
160_D163_L0.6490.99
227_I237_T0.6490.99
248_T253_L0.6490.99
222_I225_L0.6470.99
102_V107_T0.6440.99
118_V186_T0.6420.99
78_G83_A0.6420.99
161_F257_V0.6410.99
71_Q74_H0.6380.99
32_S235_K0.6380.99
179_L238_A0.6360.99
222_I241_V0.6340.99
23_L46_A0.6330.99
138_G251_A0.6310.99
71_Q79_D0.6310.99
15_L19_V0.6290.99
18_D22_A0.6250.99
21_A203_G0.6230.99
195_K256_A0.6230.99
202_L242_K0.6220.99
71_Q77_G0.6200.99
84_I87_G0.6180.99
17_P20_L0.6180.99
82_E86_E0.6160.99
165_T257_V0.6160.99
78_G125_P0.6130.99
183_I255_V0.6130.99
106_L155_L0.6100.99
19_V23_L0.6060.99
134_A266_H0.6050.99
48_I54_L0.6040.99
110_N113_D0.6010.99
82_E89_R0.6010.99
106_L211_L0.6010.99
69_I77_G0.5970.99
14_Y17_P0.5940.99
46_A56_I0.5930.99
62_T186_T0.5920.99
181_V243_I0.5900.99
147_Y168_T0.5890.99
104_D208_D0.5890.99
147_Y165_T0.5850.99
38_V229_P0.5840.99
74_H123_I0.5840.99
66_F122_E0.5810.98
41_Q44_E0.5800.98
26_A239_L0.5800.98
42_I46_A0.5770.98
186_T189_K0.5770.98
118_V246_L0.5760.98
69_I75_I0.5750.98
13_Y97_L0.5750.98
146_L190_A0.5740.98
82_E125_P0.5720.98
151_P154_G0.5710.98
30_E35_A0.5710.98
8_C88_V0.5710.98
23_L27_L0.5710.98
102_V250_I0.5670.98
230_Q260_S0.5650.98
38_V41_Q0.5640.98
214_L217_E0.5640.98
163_L224_A0.5620.98
67_V119_I0.5620.98
49_A56_I0.5620.98
158_V253_L0.5570.98
132_T135_P0.5560.98
143_K177_G0.5550.98
18_D201_P0.5540.98
201_P242_K0.5530.98
200_R242_K0.5520.98
149_L154_G0.5520.98
30_E236_T0.5520.98
75_I80_L0.5510.98
90_R93_T0.5470.98
34_N37_D0.5470.98
4_V131_I0.5430.98
145_A169_A0.5420.98
71_Q126_G0.5400.98
8_C135_P0.5370.97
23_L43_L0.5370.97
147_Y153_D0.5350.97
64_V188_E0.5350.97
218_L241_V0.5310.97
67_V75_I0.5300.97
158_V162_V0.5290.97
106_L158_V0.5290.97
229_P234_G0.5280.97
16_P266_H0.5270.97
170_G175_P0.5260.97
200_R215_E0.5250.97
111_T247_P0.5220.97
80_L129_L0.5220.97
236_T239_L0.5210.97
62_T252_S0.5200.97
101_V115_T0.5190.97
162_V222_I0.5110.97
155_L218_L0.5110.97
18_D266_H0.5100.97
260_S263_A0.5090.96
214_L221_K0.5060.96
7_L11_A0.5050.96
226_G233_G0.5030.96
101_V118_V0.5030.96
64_V118_V0.5020.96
2_E5_A0.5020.96
38_V230_Q0.5020.96
140_S264_D0.5010.96
191_A194_A0.5000.96
42_I263_A0.5000.96
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3k9dA 2 0.881 60.6 0.958 Contact Map
4knaA 2 0.9182 47.1 0.962 Contact Map
3kzqA 3 0.3197 41.8 0.963 Contact Map
4gxzA 1 0.3197 37.2 0.964 Contact Map
3rppA 2 0.3494 35.8 0.964 Contact Map
3ty7A 2 0.9182 34.6 0.964 Contact Map
4z7xA 1 0.3011 34 0.965 Contact Map
1r4wA 2 0.3532 33.7 0.965 Contact Map
3ne8A 2 0.368 28.3 0.966 Contact Map
3h6eA 1 0.3048 27.3 0.966 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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