GREMLIN Database
Thermopsin - Thermopsin
PFAM: PF05317 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 259 (253)
Sequences: 513 (417)
Seq/√Len: 26.2
META: 0.805

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
38_S239_Y4.0741.00
82_V191_F3.8011.00
65_L231_L3.3891.00
166_G175_W3.2651.00
34_Y39_V3.1281.00
67_A151_I3.0961.00
165_F179_V2.9861.00
101_I179_V2.8851.00
34_Y193_V2.7871.00
242_V245_A2.7501.00
41_G209_L2.6510.99
20_Y250_S2.6070.99
67_A207_A2.6060.99
38_S152_N2.5580.99
66_N83_Q2.5370.99
48_L61_A2.5300.99
151_I191_F2.4260.99
82_V151_I2.3500.98
39_V207_A2.2520.98
68_V104_F2.1750.97
204_P251_D2.1660.97
105_S200_P2.1630.97
39_V193_V2.1190.97
79_Y205_Y2.1120.97
45_I227_A2.1060.97
84_N176_Y2.0230.96
86_A147_L2.0000.96
162_E180_T1.9970.96
66_N252_T1.9580.95
8_Y13_A1.9380.95
199_T203_L1.9050.94
193_V207_A1.8860.94
99_D220_T1.8750.94
52_N220_T1.8690.94
209_L229_L1.8570.93
19_D32_Y1.8550.93
219_T255_T1.8220.93
35_N192_L1.8200.93
33_S194_S1.8190.93
116_I122_V1.7980.92
97_F247_N1.7830.92
45_I48_L1.7730.92
215_G218_S1.7670.92
99_D177_D1.7410.91
199_T205_Y1.7350.91
123_Y133_A1.7200.90
222_F225_L1.7080.90
84_N116_I1.6150.87
43_V147_L1.5880.86
64_Q83_Q1.5670.86
68_V79_Y1.5540.85
16_G21_G1.5540.85
83_Q254_E1.5430.85
48_L211_L1.5280.84
22_L31_P1.5040.83
214_P218_S1.4670.82
42_T148_Y1.4620.81
61_A211_L1.4540.81
18_A250_S1.4310.80
222_F256_A1.4230.80
191_F195_G1.4100.79
167_Y247_N1.3930.78
112_S115_T1.3740.77
48_L227_A1.3600.76
24_N246_Y1.3460.76
97_F167_Y1.3420.75
79_Y200_P1.3310.75
85_V214_P1.3240.74
192_L205_Y1.3220.74
71_V181_F1.3060.73
213_G254_E1.2980.73
85_V215_G1.2980.73
17_I48_L1.2930.73
149_L209_L1.2920.72
234_W238_N1.2850.72
61_A88_F1.2830.72
42_T232_Y1.2810.72
198_Y204_P1.2730.71
24_N29_L1.2710.71
164_Y178_N1.2710.71
235_N240_Q1.2540.70
19_D242_V1.2460.70
203_L251_D1.2430.69
211_L227_A1.2430.69
60_G214_P1.2400.69
14_P249_G1.2350.69
100_N132_Y1.2350.69
70_V192_L1.2300.69
100_N165_F1.2220.68
83_Q252_T1.2140.68
23_G31_P1.2100.67
21_G32_Y1.2050.67
150_Y166_G1.1930.66
84_N151_I1.1600.64
101_I165_F1.1490.63
119_N123_Y1.1460.63
54_S220_T1.1420.63
154_S164_Y1.1390.63
123_Y132_Y1.1290.62
19_D34_Y1.1230.61
168_S172_G1.1220.61
202_G208_E1.1200.61
255_T258_N1.1170.61
33_S196_Y1.1090.60
26_S29_L1.1070.60
83_Q213_G1.0950.59
64_Q254_E1.0910.59
82_V101_I1.0840.59
66_N254_E1.0760.58
45_I63_L1.0710.58
163_V179_V1.0690.58
194_S197_N1.0580.57
17_I220_T1.0510.56
88_F211_L1.0490.56
85_V98_V1.0450.56
20_Y204_P1.0450.56
249_G254_E1.0440.56
64_Q213_G1.0440.56
175_W178_N1.0390.55
44_N146_T1.0320.55
48_L225_L1.0320.55
66_N213_G1.0300.55
168_S173_S1.0180.54
56_P109_A1.0150.54
50_A61_A1.0140.54
87_F98_V1.0140.54
109_A112_S0.9970.52
101_I116_I0.9960.52
39_V242_V0.9950.52
234_W247_N0.9890.52
68_V251_D0.9890.52
17_I231_L0.9890.52
230_A241_P0.9870.52
61_A222_F0.9830.51
43_V63_L0.9680.50
125_S128_G0.9670.50
43_V47_S0.9630.50
87_F96_S0.9630.50
94_T189_A0.9620.50
63_L211_L0.9600.50
249_G252_T0.9580.49
95_L151_I0.9540.49
166_G173_S0.9530.49
35_N190_Y0.9480.49
123_Y127_G0.9370.48
84_N224_S0.9310.47
62_S116_I0.9310.47
105_S189_A0.9280.47
6_P204_P0.9150.46
62_S98_V0.9060.46
230_A245_A0.9050.45
63_L143_L0.9020.45
68_V199_T0.9000.45
62_S130_Y0.8980.45
84_N99_D0.8920.44
96_S137_T0.8890.44
8_Y14_P0.8860.44
65_L242_V0.8820.44
24_N27_G0.8710.43
69_L250_S0.8690.43
13_A250_S0.8680.43
62_S212_G0.8670.43
209_L212_G0.8650.43
231_L247_N0.8600.42
71_V80_Y0.8550.42
88_F166_G0.8540.42
63_L141_L0.8540.42
130_Y134_Y0.8530.42
81_W208_E0.8510.42
231_L242_V0.8490.41
88_F93_N0.8450.41
100_N131_Y0.8430.41
50_A220_T0.8400.41
240_Q256_A0.8360.40
81_W206_D0.8350.40
234_W237_G0.8300.40
90_T169_V0.8270.40
136_P218_S0.8260.40
14_P18_A0.8250.40
35_N67_A0.8250.40
231_L243_P0.8190.39
81_W202_G0.8150.39
18_A229_L0.8140.39
105_S181_F0.8100.39
220_T255_T0.8090.39
17_I69_L0.7990.38
118_G179_V0.7980.38
116_I209_L0.7980.38
171_G244_S0.7970.38
80_Y181_F0.7960.38
45_I141_L0.7920.37
42_T230_A0.7870.37
47_S211_L0.7840.37
95_L138_Y0.7830.37
10_S24_N0.7780.36
14_P20_Y0.7690.36
131_Y195_G0.7630.35
42_T210_V0.7590.35
65_L149_L0.7570.35
31_P196_Y0.7570.35
34_Y222_F0.7540.35
20_Y212_G0.7490.34
19_D118_G0.7480.34
82_V163_V0.7480.34
8_Y255_T0.7470.34
18_A21_G0.7440.34
130_Y133_A0.7430.34
12_P19_D0.7430.34
64_Q100_N0.7390.34
64_Q85_V0.7390.34
61_A112_S0.7380.34
22_L29_L0.7350.33
19_D63_L0.7320.33
51_Y115_T0.7270.33
121_T131_Y0.7200.32
19_D244_S0.7190.32
42_T255_T0.7180.32
141_L145_F0.7160.32
92_T107_P0.7120.32
206_D216_G0.7080.32
108_S230_A0.7080.32
82_V165_F0.7060.32
154_S218_S0.7050.31
81_W213_G0.7050.31
20_Y117_S0.7030.31
15_M19_D0.7020.31
52_N61_A0.6990.31
70_V194_S0.6970.31
105_S207_A0.6950.31
50_A90_T0.6950.31
31_P195_G0.6930.31
105_S153_V0.6910.31
18_A208_E0.6900.31
105_S247_N0.6890.30
92_T128_G0.6880.30
86_A210_V0.6850.30
156_T162_E0.6840.30
20_Y248_F0.6830.30
26_S222_F0.6730.29
222_F227_A0.6710.29
63_L186_G0.6650.29
126_G129_G0.6600.29
51_Y59_Y0.6600.29
80_Y143_L0.6600.29
222_F231_L0.6590.29
61_A193_V0.6590.29
165_F214_P0.6590.29
156_T161_P0.6550.28
25_N29_L0.6530.28
25_N28_S0.6520.28
110_N198_Y0.6510.28
63_L88_F0.6490.28
85_V216_G0.6490.28
71_V183_S0.6480.28
62_S224_S0.6480.28
97_F111_L0.6450.28
53_S211_L0.6410.28
82_V161_P0.6400.27
18_A87_F0.6390.27
63_L227_A0.6380.27
145_F227_A0.6370.27
118_G165_F0.6360.27
44_N211_L0.6360.27
81_W104_F0.6360.27
26_S41_G0.6320.27
115_T118_G0.6320.27
45_I145_F0.6320.27
204_P248_F0.6310.27
113_N246_Y0.6310.27
69_L163_V0.6290.27
7_L10_S0.6290.27
63_L222_F0.6290.27
87_F214_P0.6290.27
92_T141_L0.6250.27
28_S238_N0.6250.27
58_P93_N0.6250.27
96_S123_Y0.6240.27
34_Y242_V0.6230.26
106_S109_A0.6210.26
75_G187_V0.6210.26
185_G188_S0.6180.26
223_N229_L0.6180.26
194_S205_Y0.6170.26
41_G65_L0.6170.26
37_S42_T0.6170.26
80_Y145_F0.6160.26
172_G235_N0.6130.26
99_D193_V0.6120.26
194_S198_Y0.6120.26
55_P214_P0.6110.26
54_S62_S0.6100.26
221_T257_S0.6090.26
53_S93_N0.5990.25
67_A82_V0.5930.25
210_V229_L0.5910.25
66_N84_N0.5900.25
64_Q252_T0.5870.24
153_V189_A0.5870.24
39_V191_F0.5850.24
105_S120_G0.5840.24
192_L224_S0.5830.24
118_G123_Y0.5830.24
43_V48_L0.5820.24
66_N81_W0.5820.24
74_G181_F0.5820.24
237_G240_Q0.5810.24
111_L210_V0.5780.24
126_G226_N0.5770.24
7_L20_Y0.5750.24
18_A209_L0.5750.24
209_L223_N0.5730.24
43_V86_A0.5720.24
79_Y231_L0.5720.24
23_G26_S0.5710.24
19_D197_N0.5700.24
36_T39_V0.5690.23
75_G150_Y0.5670.23
111_L134_Y0.5650.23
166_G174_G0.5630.23
150_Y173_S0.5630.23
98_V212_G0.5630.23
166_G239_Y0.5620.23
109_A212_G0.5590.23
166_G178_N0.5590.23
110_N113_N0.5570.23
38_S150_Y0.5550.23
57_G211_L0.5540.23
33_S192_L0.5540.23
84_N242_V0.5530.23
225_L240_Q0.5490.22
87_F210_V0.5490.22
51_Y216_G0.5480.22
214_P217_G0.5480.22
38_S154_S0.5470.22
164_Y175_W0.5470.22
62_S196_Y0.5470.22
39_V202_G0.5470.22
19_D194_S0.5460.22
144_P243_P0.5450.22
149_L212_G0.5440.22
231_L245_A0.5440.22
99_D132_Y0.5430.22
67_A96_S0.5410.22
144_P208_E0.5410.22
43_V69_L0.5410.22
90_T93_N0.5400.22
117_S123_Y0.5400.22
105_S157_S0.5370.22
86_A92_T0.5360.22
97_F136_P0.5330.22
126_G132_Y0.5320.22
108_S111_L0.5310.22
90_T246_Y0.5300.21
21_G193_V0.5260.21
213_G252_T0.5250.21
80_Y163_V0.5240.21
50_A143_L0.5230.21
45_I241_P0.5220.21
137_T188_S0.5210.21
93_N140_T0.5210.21
186_G189_A0.5200.21
70_V193_V0.5200.21
172_G227_A0.5170.21
106_S247_N0.5170.21
101_I224_S0.5160.21
49_S228_T0.5150.21
59_Y90_T0.5110.21
146_T167_Y0.5100.20
49_S223_N0.5080.20
97_F170_D0.5080.20
142_S180_T0.5070.20
234_W240_Q0.5070.20
21_G177_D0.5060.20
116_I150_Y0.5060.20
144_P227_A0.5040.20
48_L151_I0.5030.20
115_T183_S0.5030.20
181_F216_G0.5020.20
68_V102_W0.5020.20
114_S126_G0.5000.20
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1m3yA 4 0.3089 2.9 0.973 Contact Map
2d9sA 2 0.166 1.8 0.975 Contact Map
2ag4A 1 0.3205 1.6 0.976 Contact Map
4nuuC 1 0.0463 1.5 0.977 Contact Map
1ac5A 1 0.3822 1.2 0.978 Contact Map
4ci9A 2 0.3745 1.1 0.978 Contact Map
3gmtA 1 0.2008 1 0.979 Contact Map
2ccvA 3 0.1313 0.9 0.98 Contact Map
1novD 1 0.0386 0.9 0.98 Contact Map
1p6xA 3 0.2278 0.9 0.98 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

Page generated in 0.0089 seconds.