GREMLIN Database
DUF721 - Protein of unknown function (DUF721)
PFAM: PF05258 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 89 (87)
Sequences: 44790 (29743)
Seq/√Len: 3188.7
META: 0.939

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
44_V54_R3.2141.00
40_H56_D2.4111.00
68_K86_D2.2101.00
49_G85_K2.1861.00
67_L70_E2.1641.00
50_T86_D2.1551.00
28_A78_A2.0711.00
63_E67_L2.0171.00
28_A31_E1.9461.00
29_W41_T1.9381.00
50_T88_R1.8391.00
65_R68_K1.8211.00
40_H60_W1.7841.00
62_Q66_L1.7621.00
31_E78_A1.6871.00
70_E74_R1.6611.00
68_K72_L1.5581.00
45_S52_T1.4741.00
72_L76_N1.4661.00
30_P38_A1.4651.00
69_P72_L1.3471.00
74_R77_E1.3411.00
52_T88_R1.3081.00
24_R28_A1.2971.00
42_R54_R1.2941.00
9_L13_L1.2691.00
30_P35_P1.2521.00
22_E26_L1.2451.00
34_G38_A1.2291.00
41_T64_L1.2281.00
11_K16_E1.1941.00
29_W33_V1.1881.00
73_E77_E1.1831.00
26_L43_P1.1661.00
58_S61_A1.1651.00
55_V61_A1.1631.00
23_A27_A1.1471.00
74_R78_A1.1231.00
76_N82_E1.1071.00
43_P54_R1.1011.00
36_A40_H1.0871.00
35_P39_A1.0661.00
70_E73_E1.0591.00
69_P73_E1.0551.00
33_V71_I1.0531.00
76_N83_A1.0481.00
28_A32_I1.0401.00
26_L30_P1.0401.00
61_A89_F1.0151.00
21_A81_E0.9561.00
62_Q65_R0.9501.00
80_G83_A0.9451.00
32_I78_A0.9371.00
59_A63_E0.9121.00
5_L9_L0.9071.00
57_S60_W0.8991.00
61_A65_R0.8841.00
43_P51_L0.8731.00
55_V64_L0.8651.00
47_R52_T0.8631.00
13_L16_E0.8521.00
82_E85_K0.8501.00
36_A39_A0.8471.00
76_N81_E0.8431.00
46_L84_V0.8351.00
63_E66_L0.8331.00
44_V52_T0.8321.00
19_G22_E0.8321.00
41_T53_V0.8261.00
21_A24_R0.8241.00
37_I60_W0.8191.00
20_L23_A0.8111.00
64_L89_F0.8101.00
35_P38_A0.8091.00
37_I67_L0.8041.00
60_W63_E0.7891.00
3_G7_P0.7721.00
19_G23_A0.7651.00
79_L82_E0.7631.00
36_A60_W0.7611.00
4_D7_P0.7601.00
75_L78_A0.7531.00
67_L74_R0.7191.00
73_E76_N0.7181.00
76_N79_L0.7151.00
68_K87_I0.7141.00
76_N84_V0.7091.00
75_L79_L0.7051.00
64_L71_I0.7051.00
30_P39_A0.7031.00
24_R27_A0.7001.00
29_W53_V0.6991.00
8_R12_E0.6951.00
15_L19_G0.6881.00
79_L83_A0.6831.00
42_R56_D0.6791.00
59_A62_Q0.6691.00
25_L51_L0.6641.00
29_W43_P0.6621.00
7_P13_L0.6611.00
33_V41_T0.6491.00
46_L51_L0.6451.00
32_I74_R0.6401.00
68_K89_F0.6371.00
55_V60_W0.6231.00
72_L87_I0.6211.00
7_P11_K0.6091.00
34_G37_I0.6061.00
23_A26_L0.5931.00
28_A79_L0.5901.00
20_L24_R0.5881.00
22_E43_P0.5871.00
37_I64_L0.5861.00
55_V89_F0.5801.00
9_L12_E0.5751.00
18_R22_E0.5641.00
45_S54_R0.5561.00
49_G86_D0.5481.00
43_P55_V0.5461.00
16_E20_L0.5451.00
65_R86_D0.5441.00
12_E15_L0.5331.00
14_G18_R0.5311.00
40_H57_S0.5281.00
25_L75_L0.5271.00
47_R50_T0.5191.00
72_L84_V0.5151.00
7_P16_E0.5001.00
9_L15_L0.5001.00
37_I41_T0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4iqjM 1 0.7303 60.7 0.773 Contact Map
2jmpA 1 0.764 55.4 0.78 Contact Map
4tpsB 1 0.8427 35.9 0.804 Contact Map
2e0gA 1 0.7865 30 0.812 Contact Map
2z4sA 1 0 25.5 0.819 Contact Map
2d28C 1 0.8764 6.7 0.86 Contact Map
4y0xA 1 0.4494 5.2 0.867 Contact Map
3lmmA 1 1 4.7 0.87 Contact Map
4j8zA 2 0.3034 4.6 0.871 Contact Map
1nh2D 1 0.9213 4.6 0.871 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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