GREMLIN Database
DUF716 - Family of unknown function (DUF716)
PFAM: PF04819 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 135 (120)
Sequences: 825 (489)
Seq/√Len: 44.6
META: 0.109

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
50_E63_R3.9311.00
21_H33_H3.7301.00
72_T122_H3.6361.00
11_F67_I3.2371.00
43_C66_L3.0931.00
25_R29_E2.8681.00
18_F40_I2.8581.00
11_F47_T2.4771.00
76_Q122_H2.3511.00
72_T76_Q2.2791.00
18_F74_F2.2131.00
46_V62_L2.1581.00
5_L10_A2.0900.99
14_E70_Q2.0500.99
11_F43_C1.9470.99
17_L20_F1.9250.99
47_T63_R1.8690.99
76_Q80_V1.8660.99
44_A115_V1.8280.99
84_P121_W1.8260.99
109_H113_M1.7880.98
70_Q74_F1.7420.98
65_S123_L1.6560.97
114_F118_Q1.6520.97
15_L40_I1.6210.97
14_E74_F1.5100.95
35_L119_F1.4600.94
62_L129_I1.4560.94
36_L74_F1.3830.93
69_L126_V1.3600.92
22_S30_G1.3250.91
55_D58_L1.2990.90
12_F44_A1.2900.90
22_S25_R1.2870.90
50_E60_E1.2310.87
80_V122_H1.2160.87
27_G30_G1.2040.86
49_L131_A1.1690.85
41_F45_L1.1540.84
101_K104_L1.1320.83
21_H74_F1.1200.82
17_L21_H1.1100.81
46_V66_L1.0990.81
12_F16_L1.0960.81
55_D131_A1.0850.80
103_D107_D1.0810.80
25_R33_H1.0660.79
46_V59_L1.0590.78
60_E63_R1.0350.77
43_C70_Q1.0270.76
77_I120_C1.0240.76
15_L41_F1.0040.74
102_W110_R1.0020.74
40_I97_E0.9950.74
10_A13_V0.9940.74
97_E121_W0.9870.73
65_S127_L0.9870.73
26_S103_D0.9860.73
102_W111_A0.9810.73
42_L83_P0.9470.70
80_V115_V0.9430.70
68_L119_F0.9320.69
35_L99_G0.9140.67
65_S130_V0.9100.67
40_I70_Q0.9040.66
11_F26_S0.8960.66
66_L77_I0.8880.65
129_I133_I0.8770.64
33_H84_P0.8690.63
9_L15_L0.8580.62
10_A70_Q0.8580.62
13_V38_L0.8560.62
28_L68_L0.8480.61
2_L133_I0.8460.61
18_F29_E0.8420.61
29_E77_I0.8360.60
7_L11_F0.8240.59
59_L114_F0.8220.59
78_G119_F0.8160.58
81_L100_V0.8120.58
27_G123_L0.8100.58
67_I101_K0.8100.58
68_L130_V0.8080.58
14_E55_D0.8080.58
76_Q118_Q0.8030.57
35_L120_C0.8010.57
43_C54_P0.7980.57
24_D32_Y0.7970.57
19_Y61_L0.7950.56
44_A76_Q0.7900.56
69_L127_L0.7700.54
9_L134_Y0.7700.54
127_L133_I0.7650.53
120_C123_L0.7630.53
8_A12_F0.7570.53
69_L75_W0.7510.52
37_L74_F0.7500.52
6_L9_L0.7470.52
16_L38_L0.7340.50
49_L98_G0.7310.50
46_V53_L0.7290.50
14_E18_F0.7270.50
43_C47_T0.7100.48
36_L81_L0.7090.48
80_V118_Q0.7060.48
71_G78_G0.7030.47
32_Y112_I0.7030.47
38_L48_L0.6980.47
18_F134_Y0.6950.47
7_L62_L0.6930.47
43_C53_L0.6800.45
41_F65_S0.6780.45
80_V102_W0.6770.45
27_G100_V0.6760.45
2_L6_L0.6720.44
55_D119_F0.6710.44
50_E76_Q0.6700.44
73_W119_F0.6680.44
20_F44_A0.6580.43
9_L64_S0.6570.43
15_L37_L0.6560.43
80_V114_F0.6560.43
13_V64_S0.6510.42
4_R59_L0.6470.42
31_H61_L0.6460.42
72_T126_V0.6450.42
29_E54_P0.6390.41
29_E84_P0.6370.41
11_F70_Q0.6350.41
102_W117_L0.6320.41
103_D122_H0.6280.40
6_L104_L0.6260.40
32_Y77_I0.6240.40
5_L47_T0.6240.40
23_H28_L0.6170.39
50_E122_H0.6150.39
79_F132_G0.6140.39
43_C72_T0.6130.39
23_H29_E0.6100.39
51_V59_L0.6050.38
28_L103_D0.5980.38
7_L28_L0.5970.37
6_L48_L0.5920.37
74_F127_L0.5890.37
12_F110_R0.5800.36
16_L31_H0.5790.36
14_E131_A0.5770.36
40_I79_F0.5750.35
3_D15_L0.5740.35
45_L55_D0.5720.35
54_P110_R0.5690.35
12_F35_L0.5650.35
42_L118_Q0.5610.34
58_L61_L0.5600.34
18_F61_L0.5540.34
71_G79_F0.5530.34
17_L33_H0.5520.33
29_E75_W0.5470.33
71_G81_L0.5440.33
45_L120_C0.5430.33
62_L114_F0.5420.33
20_F36_L0.5400.32
14_E36_L0.5400.32
49_L52_W0.5390.32
14_E20_F0.5380.32
42_L123_L0.5380.32
74_F81_L0.5330.32
2_L134_Y0.5310.32
4_R63_R0.5280.31
65_S132_G0.5180.31
8_A111_A0.5160.30
3_D49_L0.5140.30
27_G110_R0.5140.30
39_V57_F0.5140.30
111_A114_F0.5130.30
68_L124_A0.5130.30
2_L58_L0.5130.30
21_H116_T0.5080.30
30_G110_R0.5070.30
78_G121_W0.5000.29
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4grvA 1 0.9852 2.4 0.952 Contact Map
4o6yA 2 0.4148 2.1 0.954 Contact Map
2nr1A 1 0.1704 1.8 0.956 Contact Map
2mofA 1 0.2148 1.5 0.957 Contact Map
4xhjA 1 0.3556 1.1 0.96 Contact Map
4ogqE 1 0.1704 1 0.961 Contact Map
2k9pA 1 0.3037 0.8 0.964 Contact Map
2qjyA 2 0.7407 0.8 0.964 Contact Map
4zwjA 1 0.9333 0.8 0.965 Contact Map
1q90L 1 0.1704 0.8 0.965 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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