GREMLIN Database
DUF599 - Protein of unknown function, DUF599
PFAM: PF04654 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 209 (204)
Sequences: 3747 (2481)
Seq/√Len: 173.7
META: 0.762

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
5_L195_L5.2541.00
52_I56_Q3.7831.00
186_G189_A3.6061.00
182_A193_A3.4081.00
38_R154_E3.2011.00
121_A173_A2.9171.00
9_A198_V2.7831.00
153_A157_A2.7651.00
182_A189_A2.7051.00
118_F177_A2.6301.00
42_R150_E2.5701.00
135_V160_L2.5671.00
113_L180_A2.5591.00
43_Q47_T2.5211.00
5_L191_I2.3261.00
70_S74_L2.3181.00
142_A152_F2.2801.00
188_W192_A2.2591.00
103_S106_L2.1761.00
44_M142_A2.0691.00
38_R42_R1.9561.00
104_R108_E1.8331.00
5_L9_A1.8151.00
79_L118_F1.8091.00
57_I140_G1.8021.00
188_W191_I1.7631.00
88_K91_E1.7621.00
64_G129_I1.7491.00
61_L137_I1.7431.00
53_L56_Q1.7281.00
41_M156_A1.7271.00
176_F198_V1.7271.00
79_L115_L1.7241.00
150_E154_E1.7181.00
202_Y207_A1.6581.00
9_A194_T1.5741.00
60_N136_A1.5741.00
39_A42_R1.5621.00
4_A8_L1.5601.00
20_R23_R1.5571.00
45_L149_A1.5371.00
44_M139_I1.5321.00
72_S174_Y1.4981.00
146_D149_A1.4691.00
110_K183_W1.4581.00
34_N161_N1.4421.00
79_L111_L1.4421.00
42_R154_E1.4261.00
6_F190_F1.4181.00
147_P150_E1.3581.00
7_L11_L1.3431.00
6_F194_T1.3211.00
57_I136_A1.3201.00
3_L7_L1.3121.00
36_Y39_A1.3121.00
18_E169_R1.3111.00
45_L156_A1.3111.00
146_D150_E1.3111.00
72_S121_A1.3031.00
137_I164_A1.2931.00
38_R157_A1.2911.00
8_L12_G1.2751.00
189_A193_A1.2651.00
16_F202_Y1.2461.00
56_Q59_R1.2321.00
122_F126_T1.2241.00
19_R22_R1.2121.00
15_L19_R1.2111.00
194_T198_V1.2071.00
128_S132_F1.2061.00
62_Q129_I1.2051.00
65_T129_I1.2011.00
90_L107_W1.1911.00
60_N63_M1.1911.00
55_V59_R1.1901.00
58_L135_V1.1871.00
52_I55_V1.1821.00
45_L142_A1.1811.00
62_Q133_N1.1781.00
199_A203_R1.1561.00
13_Y198_V1.1541.00
39_A43_Q1.1521.00
125_F170_G1.1231.00
36_Y137_I1.1201.00
75_A131_L1.1191.00
16_F21_A1.1191.00
44_M51_R1.1041.00
75_A174_Y1.0881.00
68_F125_F1.0831.00
138_L156_A1.0821.00
152_F156_A1.0701.00
111_L115_L1.0691.00
38_R158_R1.0631.00
68_F122_F1.0581.00
9_A12_G1.0470.99
76_I115_L1.0470.99
50_N55_V1.0450.99
114_L180_A1.0440.99
32_L36_Y1.0300.99
95_D104_R1.0260.99
19_R23_R1.0250.99
80_L115_L1.0190.99
132_F163_A1.0120.99
80_L122_F1.0070.99
11_L15_L1.0050.99
16_F22_R1.0030.99
77_G81_A1.0020.99
68_F175_Y0.9770.99
126_T175_Y0.9750.99
154_E158_R0.9710.99
50_N53_L0.9580.99
49_E144_N0.9520.99
14_H17_L0.9520.99
114_L118_F0.9370.99
66_S129_I0.9370.99
42_R153_A0.9360.99
47_T50_N0.9330.99
87_D104_R0.9300.99
74_L78_G0.9110.99
65_T133_N0.8910.98
20_R24_R0.8910.98
132_F164_A0.8890.98
81_A84_G0.8870.98
48_R51_R0.8810.98
157_A161_N0.8800.98
58_L139_I0.8790.98
65_T167_F0.8750.98
191_I195_L0.8740.98
6_F117_I0.8670.98
22_R25_P0.8670.98
117_I180_A0.8650.98
86_T90_L0.8630.98
102_T106_L0.8570.98
8_L11_L0.8570.98
135_V156_A0.8570.98
3_L6_F0.8560.98
65_T128_S0.8500.98
12_G16_F0.8470.98
71_T174_Y0.8420.98
30_S205_E0.8390.98
118_F174_Y0.8320.97
62_Q175_Y0.8240.97
145_D149_A0.8180.97
192_A196_L0.8150.97
147_P151_A0.8120.97
35_R38_R0.8100.97
168_N172_R0.8080.97
6_F113_L0.8060.97
141_A152_F0.8030.97
61_L132_F0.8030.97
17_L21_A0.8020.97
48_R54_D0.7930.97
131_L163_A0.7870.96
106_L110_K0.7870.96
41_M44_M0.7870.96
43_Q144_N0.7780.96
92_V102_T0.7680.96
47_T150_E0.7680.96
183_W190_F0.7670.96
80_L178_L0.7630.96
81_A107_W0.7630.96
129_I133_N0.7610.96
191_I194_T0.7600.96
87_D91_E0.7510.95
30_S165_R0.7440.95
79_L114_L0.7400.95
138_L159_L0.7390.95
145_D148_E0.7380.95
102_T105_L0.7330.95
90_L104_R0.7330.95
100_G187_P0.7310.95
41_M153_A0.7300.95
13_Y17_L0.7280.95
187_P190_F0.7280.95
8_L47_T0.7270.95
204_R208_S0.7230.94
56_Q63_M0.7210.94
53_L139_I0.7030.93
120_Y173_A0.7030.93
61_L128_S0.7000.93
55_V160_L0.6990.93
85_S89_A0.6990.93
65_T202_Y0.6910.93
73_I174_Y0.6890.93
43_Q66_S0.6860.93
64_G137_I0.6850.93
61_L163_A0.6830.92
51_R54_D0.6830.92
58_L136_A0.6810.92
132_F160_L0.6790.92
88_K95_D0.6780.92
68_F124_K0.6720.92
90_L100_G0.6720.92
158_R162_R0.6690.92
166_H203_R0.6670.92
57_I196_L0.6670.92
118_F133_N0.6660.92
114_L184_F0.6650.91
133_N167_F0.6610.91
114_L154_E0.6580.91
200_V203_R0.6550.91
133_N136_A0.6510.91
74_L122_F0.6490.90
124_K173_A0.6480.90
43_Q50_N0.6460.90
68_F126_T0.6460.90
47_T144_N0.6450.90
2_A6_F0.6450.90
106_L109_L0.6430.90
124_K127_W0.6420.90
47_T51_R0.6400.90
16_F20_R0.6380.90
162_R165_R0.6330.89
12_G22_R0.6320.89
36_Y60_N0.6310.89
63_M66_S0.6310.89
196_L199_A0.6260.89
142_A162_R0.6250.89
35_R39_A0.6250.89
9_A13_Y0.6240.89
77_G137_I0.6230.89
131_L159_L0.6230.89
64_G77_G0.6210.88
136_A164_A0.6200.88
60_N205_E0.6200.88
91_E101_A0.6160.88
95_D105_L0.6160.88
6_F9_A0.6140.88
186_G196_L0.6130.88
4_A7_L0.6120.88
61_L136_A0.6080.87
84_G181_L0.6070.87
124_K166_H0.6050.87
112_L116_L0.6030.87
64_G131_L0.6020.87
75_A79_L0.6010.87
59_R62_Q0.5940.86
28_S31_G0.5940.86
9_A24_R0.5940.86
171_L174_Y0.5930.86
95_D108_E0.5910.86
64_G71_T0.5890.86
66_S69_A0.5780.85
77_G127_W0.5770.85
53_L63_M0.5750.84
25_P151_A0.5740.84
67_F164_A0.5730.84
11_L100_G0.5730.84
33_M134_Y0.5720.84
57_I86_T0.5700.84
17_L28_S0.5700.84
59_R63_M0.5680.84
41_M157_A0.5650.83
133_N175_Y0.5650.83
199_A202_Y0.5620.83
81_A95_D0.5600.83
61_L75_A0.5600.83
53_L57_I0.5600.83
72_S122_F0.5590.83
135_V138_L0.5590.83
127_W131_L0.5560.82
185_F189_A0.5530.82
86_T94_S0.5530.82
197_V205_E0.5510.82
45_L144_N0.5500.82
52_I60_N0.5500.82
13_Y21_A0.5490.82
161_N165_R0.5490.82
158_R161_N0.5460.81
101_A106_L0.5440.81
76_I163_A0.5440.81
14_H18_E0.5440.81
65_T136_A0.5430.81
64_G133_N0.5420.81
111_L184_F0.5400.80
66_S136_A0.5370.80
140_G163_A0.5350.80
201_L205_E0.5350.80
37_R164_A0.5340.80
42_R45_L0.5340.80
99_A119_V0.5320.79
144_N147_P0.5240.78
150_E153_A0.5240.78
132_F167_F0.5220.78
32_L35_R0.5200.78
89_A107_W0.5190.78
58_L160_L0.5190.78
126_T129_I0.5180.78
60_N126_T0.5170.78
75_A118_F0.5170.78
63_M112_L0.5170.78
82_L89_A0.5150.77
67_F139_I0.5140.77
202_Y205_E0.5130.77
105_L147_P0.5120.77
21_A24_R0.5120.77
189_A196_L0.5090.76
44_M53_L0.5080.76
164_A205_E0.5080.76
5_L190_F0.5080.76
14_H169_R0.5040.76
88_K108_E0.5020.75
19_R196_L0.5020.75
190_F194_T0.5010.75
13_Y205_E0.5000.75
155_R164_A0.5000.75
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3wajA 1 0.4402 5.6 0.959 Contact Map
2l2tA 2 0.134 3.4 0.963 Contact Map
2ki9A 1 0.1148 3 0.964 Contact Map
2z73A 2 0.2871 2.6 0.965 Contact Map
2ks1B 1 0.1292 2.3 0.966 Contact Map
2fyuK 1 0.2536 2.1 0.967 Contact Map
2jwaA 2 0.1244 2 0.967 Contact Map
1qleC 1 0.2871 1.8 0.968 Contact Map
2bg9A 1 0.7847 1.8 0.968 Contact Map
4il3A 1 0.4785 1.6 0.969 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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