GREMLIN Database
DUF563 - Protein of unknown function (DUF563)
PFAM: PF04577 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 208 (201)
Sequences: 11163 (10000)
Seq/√Len: 705.4
META: 0.796

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
160_G166_L3.5291.00
172_C176_T2.6931.00
177_K201_D2.6821.00
144_S147_E2.6721.00
127_E137_I2.5561.00
106_R156_E2.4591.00
111_S139_D2.4481.00
135_F157_V2.4181.00
129_L133_Y2.3901.00
130_L159_V2.2911.00
140_P152_F2.2731.00
114_D141_E2.2421.00
162_H180_E2.2381.00
106_R136_E2.1941.00
2_N164_A2.1481.00
42_E146_A2.1391.00
157_V177_K2.1361.00
89_R93_R2.1041.00
6_W43_S2.0741.00
155_A158_I2.0241.00
139_D142_D1.9951.00
105_E156_E1.9831.00
107_K135_F1.9821.00
109_Y123_E1.9591.00
43_S145_F1.9101.00
138_V151_L1.8951.00
143_L151_L1.8571.00
107_K156_E1.8451.00
108_I155_A1.8281.00
65_V68_L1.8261.00
3_Y168_N1.8191.00
4_G167_T1.7761.00
150_A154_N1.7001.00
111_S123_E1.6751.00
123_E127_E1.6681.00
7_L171_F1.6411.00
5_H9_D1.6211.00
106_R154_N1.6141.00
145_F149_I1.5941.00
180_E202_Y1.5911.00
192_W196_S1.5811.00
51_P54_R1.5651.00
110_I165_G1.5621.00
157_V179_I1.5231.00
147_E150_A1.5151.00
176_T200_L1.5101.00
68_L71_P1.5061.00
114_D139_D1.5051.00
38_P42_E1.4941.00
122_N125_E1.4911.00
166_L178_V1.4731.00
127_E131_E1.4671.00
110_I152_F1.4261.00
152_F158_I1.4211.00
172_C200_L1.4101.00
138_V143_L1.4011.00
123_E161_P1.3981.00
37_P40_Q1.3931.00
152_F172_C1.3771.00
111_S137_I1.3641.00
107_K157_V1.3371.00
177_K203_Y1.3351.00
114_D142_D1.2941.00
149_I171_F1.2931.00
160_G165_G1.2871.00
42_E45_E1.2771.00
46_L146_A1.2571.00
4_G164_A1.2531.00
195_A200_L1.2521.00
158_I172_C1.2411.00
18_E91_R1.2331.00
4_G8_L1.2271.00
178_V200_L1.2221.00
54_R58_D1.2191.00
146_A150_A1.2091.00
169_L178_V1.1971.00
15_L19_E1.1821.00
195_A202_Y1.1781.00
68_L72_S1.1761.00
113_R139_D1.1741.00
28_L31_P1.1481.00
91_R95_R1.1391.00
143_L147_E1.1251.00
32_S58_D1.1211.00
21_G24_P1.0991.00
135_F159_V1.0921.00
7_L167_T1.0861.00
153_S173_P1.0851.00
129_L132_K1.0761.00
128_E131_E1.0741.00
140_P143_L1.0741.00
27_P30_V1.0571.00
111_S127_E1.0541.00
122_N126_L1.0461.00
186_Y190_C1.0451.00
7_L145_F1.0421.00
120_I161_P1.0271.00
143_L148_Q1.0251.00
109_Y159_V1.0181.00
88_L91_R1.0171.00
131_E134_G0.9981.00
192_W202_Y0.9971.00
63_V66_E0.9911.00
110_I140_P0.9911.00
89_R92_L0.9821.00
15_L18_E0.9791.00
169_L195_A0.9771.00
42_E144_S0.9701.00
86_R90_D0.9681.00
113_R137_I0.9671.00
112_R163_G0.9581.00
85_L89_R0.9581.00
20_L23_D0.9351.00
20_L24_P0.9231.00
90_D94_E0.9231.00
93_R153_S0.9231.00
179_I205_L0.9221.00
36_L41_R0.9201.00
26_D29_L0.9181.00
17_L20_L0.9171.00
182_F204_Y0.9151.00
36_L40_Q0.9141.00
136_E151_L0.9131.00
169_L172_C0.9111.00
8_L167_T0.9081.00
164_A167_T0.9051.00
174_P199_G0.9031.00
148_Q168_N0.8931.00
128_E132_K0.8871.00
109_Y120_I0.8791.00
152_F168_N0.8761.00
32_S35_D0.8691.00
167_T191_Y0.8691.00
126_L129_L0.8681.00
112_R118_R0.8671.00
159_V179_I0.8661.00
69_I72_S0.8651.00
11_L43_S0.8621.00
173_P176_T0.8591.00
145_F148_Q0.8571.00
140_P165_G0.8501.00
76_P79_G0.8471.00
130_L135_F0.8471.00
87_W91_R0.8471.00
166_L180_E0.8441.00
104_P156_E0.8431.00
16_L20_L0.8331.00
82_P86_R0.8301.00
6_W39_F0.8291.00
192_W204_Y0.8241.00
110_I158_I0.8231.00
81_S84_L0.8201.00
152_F155_A0.8191.00
137_I140_P0.8181.00
123_E137_I0.8011.00
109_Y130_L0.7981.00
93_R97_G0.7981.00
8_L170_L0.7951.00
7_L11_L0.7941.00
21_G25_D0.7941.00
173_P200_L0.7921.00
109_Y127_E0.7921.00
79_G82_P0.7891.00
108_I151_L0.7861.00
77_S80_Y0.7861.00
105_E154_N0.7841.00
140_P148_Q0.7831.00
105_E136_E0.7821.00
87_W90_D0.7801.00
91_R94_E0.7791.00
156_E173_P0.7711.00
89_R198_L0.7701.00
80_Y84_L0.7691.00
113_R127_E0.7691.00
83_P86_R0.7691.00
81_S85_L0.7681.00
193_N197_A0.7631.00
8_L194_L0.7621.00
82_P85_L0.7601.00
125_E128_E0.7481.00
158_I169_L0.7461.00
189_P192_W0.7431.00
17_L21_G0.7401.00
22_P25_D0.7401.00
49_I52_E0.7391.00
46_L95_R0.7381.00
148_Q152_F0.7351.00
149_I153_S0.7231.00
169_L200_L0.7221.00
175_G200_L0.7181.00
159_V181_L0.7141.00
131_E137_I0.7041.00
107_K136_E0.7021.00
110_I160_G0.6991.00
182_F187_V0.6961.00
41_R45_E0.6921.00
62_P65_V0.6901.00
182_F186_Y0.6891.00
72_S75_F0.6791.00
53_D56_V0.6791.00
168_N171_F0.6771.00
107_K133_Y0.6751.00
97_G153_S0.6731.00
130_L134_G0.6711.00
124_D128_E0.6711.00
115_A119_R0.6691.00
58_D61_E0.6671.00
105_E108_I0.6631.00
166_L191_Y0.6621.00
187_V190_C0.6591.00
65_V69_I0.6571.00
42_E46_L0.6471.00
113_R123_E0.6471.00
186_Y189_P0.6461.00
126_L130_L0.6391.00
81_S86_R0.6381.00
4_G190_C0.6371.00
194_L198_L0.6371.00
108_I138_V0.6361.00
6_W40_Q0.6361.00
130_L133_Y0.6351.00
127_E132_K0.6321.00
7_L43_S0.6321.00
185_G188_N0.6311.00
27_P53_D0.6271.00
38_P41_R0.6271.00
15_L21_G0.6231.00
88_L92_L0.6221.00
67_R71_P0.6221.00
93_R96_L0.6191.00
157_V161_P0.6151.00
193_N196_S0.6141.00
80_Y85_L0.6131.00
15_L20_L0.6111.00
19_E87_W0.6101.00
124_D127_E0.6081.00
120_I181_L0.6071.00
41_R52_E0.6011.00
92_L96_L0.6011.00
45_E48_G0.6011.00
29_L65_V0.6001.00
106_R135_F0.5981.00
13_R73_P0.5951.00
45_E146_A0.5941.00
59_P62_P0.5921.00
162_H182_F0.5901.00
162_H191_Y0.5901.00
179_I203_Y0.5901.00
18_E21_G0.5871.00
78_G82_P0.5851.00
67_R72_S0.5851.00
102_S154_N0.5851.00
20_L25_D0.5811.00
3_Y167_T0.5811.00
4_G191_Y0.5801.00
112_R164_A0.5781.00
90_D93_R0.5771.00
58_D63_V0.5711.00
162_H202_Y0.5701.00
80_Y83_P0.5691.00
93_R173_P0.5681.00
9_D13_R0.5681.00
31_P35_D0.5661.00
59_P63_V0.5651.00
44_L49_I0.5581.00
85_L88_L0.5581.00
172_C178_V0.5561.00
189_P193_N0.5561.00
191_Y194_L0.5551.00
13_R71_P0.5551.00
76_P83_P0.5541.00
63_V68_L0.5501.00
156_E177_K0.5491.00
150_A153_S0.5491.00
182_F190_C0.5491.00
118_R164_A0.5471.00
32_S56_V0.5471.00
136_E154_N0.5471.00
79_G84_L0.5471.00
151_L155_A0.5461.00
90_D198_L0.5431.00
77_S81_S0.5401.00
27_P31_P0.5381.00
76_P80_Y0.5381.00
45_E52_E0.5371.00
49_I53_D0.5361.00
120_I126_L0.5361.00
88_L93_R0.5331.00
53_D60_D0.5321.00
54_R57_V0.5311.00
63_V67_R0.5261.00
107_K134_G0.5221.00
7_L12_P0.5221.00
191_Y195_A0.5211.00
39_F42_E0.5191.00
45_E51_P0.5161.00
161_P183_P0.5131.00
162_H165_G0.5131.00
183_P186_Y0.5090.99
144_S148_Q0.5080.99
110_I166_L0.5060.99
162_H166_L0.5000.99
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4pxyA 4 0.5385 13.9 0.927 Contact Map
4jelA 2 0.5096 10 0.932 Contact Map
4mcjA 3 0.6058 8.9 0.933 Contact Map
4jemA 2 0.5096 7.8 0.935 Contact Map
4ohrA 2 0.5048 6.3 0.938 Contact Map
3sozA 3 0.5 6 0.938 Contact Map
1iugA 2 0.476 5.7 0.939 Contact Map
2g76A 2 0.4567 5.4 0.94 Contact Map
1wwkA 2 0.4519 4.9 0.941 Contact Map
4e5vA 1 0.4952 4.8 0.941 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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