GREMLIN Database
SPC22 - Signal peptidase subunit
PFAM: PF04573 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 168 (166)
Sequences: 550 (397)
Seq/√Len: 30.8
META: 0.255

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
67_D115_D5.7781.00
144_N152_L3.7661.00
88_E98_Q2.9581.00
65_D143_W2.8691.00
70_L106_I2.5260.99
94_N128_Q2.4070.99
14_T18_V2.4040.99
101_L125_F2.2690.99
109_K112_A2.1720.98
80_Q105_I2.1130.98
81_L145_V2.0930.98
103_D123_Y2.0400.98
74_F82_F2.0090.97
103_D122_K1.8750.96
23_I27_T1.8500.96
67_D113_K1.7920.95
144_N154_Y1.7870.95
64_F114_L1.7720.95
2_L5_A1.7710.95
10_A20_A1.7560.94
152_L155_G1.7470.94
116_L119_V1.7450.94
6_N10_A1.6840.93
63_T134_G1.6600.92
18_V91_T1.6570.92
47_V50_F1.6480.92
90_E136_E1.6080.91
86_T142_H1.5860.90
11_F15_V1.5750.90
62_L101_L1.5630.90
70_L112_A1.5610.90
94_N129_G1.5460.89
147_P150_G1.5410.89
88_E140_T1.5380.89
5_A9_F1.5370.89
113_K117_K1.5050.88
6_N146_M1.4800.87
6_N16_L1.4740.87
98_Q142_H1.4700.87
73_L78_T1.4580.86
46_N50_F1.4270.85
84_Y147_P1.4200.85
132_L137_V1.4180.85
74_F80_Q1.4090.84
27_T75_N1.4060.84
90_E95_S1.4020.84
47_V59_L1.3960.84
143_W156_E1.3740.83
108_R112_A1.3680.82
131_G135_K1.3570.82
20_A27_T1.3530.82
89_Y99_V1.3520.82
37_I106_I1.3470.81
76_W97_N1.3190.80
102_W147_P1.2910.79
3_S20_A1.2880.78
74_F112_A1.2670.77
10_A13_L1.2620.77
63_T119_V1.2570.77
64_F84_Y1.2520.76
108_R111_D1.2510.76
76_W103_D1.2490.76
28_F92_P1.2440.76
127_D143_W1.2280.75
91_T127_D1.2270.75
3_S58_D1.2220.75
85_L141_L1.2210.75
80_Q146_M1.2040.73
58_D132_L1.1940.73
45_K49_D1.1870.72
9_F15_V1.1780.72
80_Q106_I1.1760.72
18_V25_L1.1720.71
73_L81_L1.1510.70
8_V21_A1.1480.70
52_Y130_N1.1110.67
65_D89_Y1.1060.67
83_V100_V1.0950.66
137_V148_N1.0740.65
56_K160_S1.0740.65
90_E138_T1.0670.64
60_A101_L1.0580.63
65_D117_K1.0420.62
46_N49_D1.0270.61
60_A106_I1.0260.61
50_F99_V1.0010.59
65_D131_G0.9980.59
65_D157_A0.9950.59
88_E96_L0.9930.58
74_F79_K0.9920.58
87_A125_F0.9850.58
89_Y127_D0.9830.58
91_T97_N0.9780.57
18_V22_L0.9710.57
88_E112_A0.9680.56
65_D119_V0.9650.56
57_N126_W0.9450.55
86_T100_V0.9400.54
56_K60_A0.9370.54
106_I161_S0.9270.53
104_K108_R0.9260.53
64_F77_N0.9250.53
73_L79_K0.9210.53
28_F44_V0.9210.53
137_V165_F0.8920.50
161_S164_T0.8800.49
140_T157_A0.8700.49
147_P152_L0.8670.48
93_K134_G0.8630.48
73_L87_A0.8480.47
100_V122_K0.8470.47
21_A111_D0.8370.46
37_I42_V0.8340.46
7_A12_A0.8280.45
69_D113_K0.8280.45
100_V129_G0.8240.45
66_L138_T0.8230.45
61_F121_N0.8210.45
36_S72_P0.8170.44
42_V95_S0.8080.44
20_A23_I0.8010.43
113_K164_T0.8000.43
27_T145_V0.8000.43
138_T158_A0.7840.42
22_L151_L0.7810.42
64_F102_W0.7810.42
62_L80_Q0.7770.41
55_K149_V0.7720.41
27_T89_Y0.7670.40
62_L106_I0.7620.40
7_A131_G0.7590.40
20_A24_A0.7590.40
146_M149_V0.7570.40
28_F162_S0.7510.39
78_T109_K0.7480.39
81_L100_V0.7380.38
45_K54_R0.7330.38
46_N140_T0.7330.38
33_P39_V0.7310.38
103_D132_L0.7300.38
47_V72_P0.7280.37
106_I116_L0.7250.37
102_W144_N0.7160.37
9_F145_V0.7150.36
128_Q149_V0.7130.36
138_T156_E0.7120.36
71_S127_D0.7100.36
13_L27_T0.7090.36
5_A17_A0.7030.36
74_F89_Y0.7020.36
72_P113_K0.6880.34
118_N163_F0.6880.34
73_L105_I0.6860.34
99_V103_D0.6840.34
152_L157_A0.6810.34
18_V40_S0.6810.34
75_N78_T0.6800.34
98_Q158_A0.6770.34
10_A16_L0.6750.34
62_L119_V0.6730.33
89_Y143_W0.6730.33
86_T110_E0.6720.33
76_W127_D0.6720.33
142_H156_E0.6720.33
48_R51_G0.6710.33
29_F138_T0.6690.33
67_D117_K0.6670.33
87_A129_G0.6660.33
12_A15_V0.6650.33
70_L75_N0.6630.33
100_V103_D0.6630.33
26_S76_W0.6630.33
126_W143_W0.6620.33
95_S104_K0.6610.33
10_A145_V0.6600.32
44_V122_K0.6590.32
23_I148_N0.6590.32
34_P114_L0.6580.32
16_L43_Q0.6560.32
70_L84_Y0.6550.32
34_P95_S0.6540.32
82_F102_W0.6460.31
27_T41_N0.6430.31
15_V115_D0.6420.31
22_L106_I0.6400.31
52_Y104_K0.6360.31
66_L86_T0.6350.31
139_L163_F0.6330.31
36_S104_K0.6320.31
18_V21_A0.6300.30
11_F126_W0.6280.30
50_F88_E0.6220.30
117_K158_A0.6170.30
78_T81_L0.6140.29
8_V13_L0.6100.29
147_P166_P0.6080.29
12_A59_L0.6060.29
10_A165_F0.6050.29
42_V129_G0.6040.29
79_K112_A0.6010.28
107_T125_F0.5960.28
62_L93_K0.5950.28
5_A32_R0.5900.28
48_R105_I0.5900.28
51_G58_D0.5890.28
106_I117_K0.5890.28
22_L92_P0.5830.27
59_L121_N0.5810.27
40_S93_K0.5780.27
71_S112_A0.5760.27
21_A54_R0.5720.27
48_R63_T0.5700.26
45_K98_Q0.5680.26
132_L155_G0.5660.26
53_S95_S0.5660.26
23_I79_K0.5660.26
139_L155_G0.5660.26
144_N148_N0.5640.26
7_A21_A0.5640.26
17_A20_A0.5640.26
55_K145_V0.5620.26
75_N145_V0.5620.26
127_D132_L0.5610.26
64_F119_V0.5570.26
3_S18_V0.5570.26
10_A144_N0.5550.26
121_N146_M0.5550.26
68_A116_L0.5530.25
8_V90_E0.5470.25
93_K109_K0.5440.25
85_L144_N0.5420.25
82_F144_N0.5390.25
93_K115_D0.5320.24
76_W123_Y0.5320.24
12_A70_L0.5310.24
64_F68_A0.5300.24
123_Y132_L0.5280.24
14_T22_L0.5270.24
33_P50_F0.5230.24
22_L35_G0.5220.24
110_E158_A0.5150.23
24_A75_N0.5130.23
6_N133_R0.5110.23
21_A35_G0.5100.23
154_Y158_A0.5100.23
30_L67_D0.5070.23
10_A59_L0.5030.22
90_E120_K0.5000.22
3_S27_T0.5000.22
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3zqxA 1 0.4821 3.6 0.96 Contact Map
2mekA 1 0.2738 3 0.962 Contact Map
4kytB 2 0.0179 2.9 0.962 Contact Map
4y3uB 1 0.0179 2.2 0.965 Contact Map
3j45G 1 0.1369 2.2 0.965 Contact Map
4jdzB 1 0.3393 2.2 0.965 Contact Map
2f68X 1 0.3214 2.2 0.965 Contact Map
4qhpA 1 0.6667 2 0.965 Contact Map
1n7lA 1 0.1429 1.9 0.966 Contact Map
2lp1A 1 0.2024 1.8 0.966 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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