GREMLIN Database
DUF554 - Protein of unknown function (DUF554)
PFAM: PF04474 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 220 (219)
Sequences: 6898 (4885)
Seq/√Len: 330.1
META: 0.825

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
72_D75_D4.4031.00
67_I214_I3.3511.00
65_G208_L3.3141.00
5_V128_F3.2881.00
14_S150_L2.9411.00
118_D124_H2.7591.00
76_R79_R2.6431.00
117_Q121_T2.5741.00
7_A157_L2.5711.00
69_E207_N2.4901.00
124_H128_F2.4891.00
9_A131_S2.2741.00
26_E30_E2.2071.00
83_W87_R2.1821.00
31_T65_G1.9791.00
111_A159_Y1.9541.00
117_Q123_D1.9351.00
42_L188_L1.8661.00
104_L141_F1.8591.00
10_I156_V1.8561.00
82_E85_E1.8521.00
47_M187_G1.8131.00
74_E206_A1.7701.00
199_E202_K1.7601.00
41_L194_G1.7571.00
63_L214_I1.7481.00
26_E29_R1.7451.00
35_A61_L1.7361.00
42_L48_A1.7021.00
51_T181_E1.6951.00
73_I206_A1.6801.00
4_I8_A1.6711.00
11_L153_A1.6631.00
3_T160_Q1.6621.00
82_E95_A1.6551.00
30_E34_Q1.6281.00
85_E96_E1.6151.00
110_M126_L1.5761.00
67_I71_L1.5701.00
3_T164_T1.5401.00
196_N202_K1.5301.00
195_L200_I1.5271.00
35_A65_G1.4931.00
74_E78_E1.4901.00
28_I70_L1.4891.00
93_R96_E1.4851.00
101_A141_F1.4851.00
176_P180_A1.4841.00
196_N201_K1.4731.00
46_S50_K1.4691.00
195_L201_K1.4391.00
36_L137_A1.4311.00
94_F151_F1.4141.00
189_L211_A1.4041.00
116_I163_I1.4041.00
25_P28_I1.4011.00
97_G145_L1.3911.00
38_L192_G1.3871.00
72_D76_R1.3751.00
153_A157_L1.3741.00
157_L161_G1.3701.00
39_A61_L1.3431.00
97_G101_A1.3301.00
75_D79_R1.3051.00
115_S119_G1.3021.00
214_I218_L1.2981.00
102_S155_P1.2871.00
147_I151_F1.2731.00
7_A153_A1.2671.00
57_V188_L1.2591.00
192_G205_V1.2591.00
27_R72_D1.2451.00
30_E199_E1.2251.00
56_I215_A1.2081.00
118_D164_T1.2081.00
166_L169_L1.2051.00
5_V124_H1.2001.00
175_T178_M1.1981.00
111_A155_P1.1841.00
69_E204_K1.1821.00
60_S214_I1.1811.00
41_L45_I1.1801.00
16_I139_I1.1761.00
9_A135_G1.1741.00
14_S18_L1.1521.00
102_S156_V1.1331.00
46_S49_L1.1321.00
5_V131_S1.1291.00
31_T69_E1.1281.00
125_S129_T1.1221.00
11_L150_L1.1211.00
61_L188_L1.1141.00
153_A156_V1.1131.00
113_L126_L1.1101.00
39_A58_I1.1101.00
56_I218_L1.1091.00
85_E95_A1.1071.00
117_Q126_L1.1061.00
18_L147_I1.1021.00
181_E219_V1.1021.00
83_W86_R1.0961.00
170_L173_F1.0891.00
171_G175_T1.0851.00
3_T7_A1.0801.00
126_L129_T1.0751.00
10_I111_A1.0711.00
215_A219_V1.0651.00
56_I60_S1.0601.00
105_F138_S1.0571.00
59_L63_L1.0561.00
31_T34_Q1.0551.00
18_L150_L1.0531.00
45_I49_L1.0461.00
104_L137_A1.0421.00
27_R69_E1.0361.00
103_L107_V1.0331.00
67_I70_L1.0321.00
185_V215_A1.0281.00
77_L206_A1.0261.00
14_S138_S1.0201.00
58_I129_T1.0131.00
162_G166_L1.0081.00
111_A115_S1.0051.00
73_I76_R1.0001.00
14_S142_A0.9971.00
135_G139_I0.9911.00
8_A12_V0.9821.00
81_G99_V0.9711.00
177_A180_A0.9611.00
21_G29_R0.9541.00
105_F139_I0.9511.00
166_L170_L0.9501.00
45_I191_L0.9441.00
39_A133_L0.9401.00
63_L67_I0.9351.00
15_L150_L0.9271.00
60_S215_A0.9231.00
190_I193_I0.9181.00
35_A208_L0.9181.00
101_A105_F0.9181.00
215_A218_L0.9131.00
78_E82_E0.9091.00
81_G206_A0.9091.00
2_G127_L0.9061.00
138_S142_A0.9021.00
174_L179_I0.8941.00
98_F102_S0.8931.00
82_E86_R0.8901.00
57_V185_V0.8861.00
40_T133_L0.8721.00
10_I105_F0.8631.00
3_T118_D0.8501.00
85_E94_F0.8491.00
53_N181_E0.8411.00
41_L191_L0.8391.00
213_L216_P0.8391.00
5_V9_A0.8331.00
28_I69_E0.8281.00
51_T57_V0.8271.00
121_T124_H0.8261.00
155_P159_Y0.8251.00
51_T184_A0.8211.00
20_L23_R0.8181.00
32_V136_F0.8181.00
42_L191_L0.8141.00
138_S149_V0.8121.00
52_E181_E0.8071.00
84_L151_F0.8051.00
59_L136_F0.8051.00
162_G165_L0.8051.00
11_L15_L0.8031.00
100_T104_L0.8021.00
102_S111_A0.8011.00
48_A191_L0.7921.00
15_L19_L0.7911.00
72_D207_N0.7901.00
94_F148_G0.7881.00
62_V136_F0.7861.00
28_I66_L0.7841.00
55_L59_L0.7831.00
115_S163_I0.7831.00
101_A104_L0.7821.00
31_T35_A0.7811.00
1_L5_V0.7801.00
79_R83_W0.7751.00
36_L62_V0.7751.00
37_G197_L0.7721.00
61_L189_L0.7711.00
131_S135_G0.7701.00
181_E184_A0.7671.00
168_G171_G0.7661.00
163_I167_A0.7601.00
4_I7_A0.7561.00
123_D126_L0.7460.99
30_E204_K0.7410.99
150_L154_I0.7410.99
156_V162_G0.7390.99
18_L142_A0.7340.99
115_S118_D0.7320.99
132_V135_G0.7300.99
59_L140_A0.7260.99
115_S160_Q0.7250.99
62_V140_A0.7200.99
42_L46_S0.7190.99
72_D79_R0.7180.99
118_D128_F0.7140.99
154_I158_V0.7130.99
129_T132_V0.7080.99
57_V181_E0.7030.99
16_I135_G0.7010.99
2_G118_D0.7000.99
40_T197_L0.6970.99
11_L14_S0.6940.99
184_A187_G0.6930.99
147_I150_L0.6890.99
206_A209_L0.6860.99
79_R82_E0.6850.99
195_L203_I0.6850.99
74_E204_K0.6840.99
193_I205_V0.6820.99
94_F97_G0.6800.99
97_G149_V0.6800.99
137_A140_A0.6760.99
169_L172_P0.6730.99
189_L212_L0.6690.99
198_L203_I0.6690.99
43_I54_I0.6660.99
1_L128_F0.6650.99
105_F135_G0.6630.99
111_A163_I0.6620.99
109_P113_L0.6590.99
191_L195_L0.6570.99
39_A43_I0.6560.99
23_R140_A0.6530.99
42_L45_I0.6530.99
101_A152_S0.6490.98
45_I48_A0.6480.98
3_T115_S0.6480.98
155_P158_V0.6470.98
116_I120_L0.6440.98
178_M216_P0.6380.98
115_S127_L0.6380.98
78_E202_K0.6370.98
48_A188_L0.6370.98
33_M137_A0.6330.98
201_K204_K0.6310.98
192_G203_I0.6290.98
1_L124_H0.6270.98
28_I31_T0.6260.98
128_F132_V0.6250.98
165_L169_L0.6240.98
53_N56_I0.6220.98
24_L28_I0.6220.98
51_T54_I0.6210.98
39_A54_I0.6210.98
16_I20_L0.6200.98
47_M183_T0.6190.98
44_G194_G0.6190.98
195_L199_E0.6170.98
168_G172_P0.6160.98
133_L137_A0.6150.98
142_A150_L0.6130.98
178_M215_A0.6130.98
52_E177_A0.6060.98
139_I143_S0.6050.98
196_N205_V0.6040.98
20_L24_L0.6030.98
115_S164_T0.6020.98
10_I106_C0.5960.97
80_L83_W0.5950.97
57_V184_A0.5930.97
34_Q203_I0.5930.97
34_Q201_K0.5930.97
44_G48_A0.5920.97
71_L76_R0.5890.97
196_N203_I0.5890.97
2_G124_H0.5880.97
190_I194_G0.5880.97
47_M126_L0.5870.97
39_A190_I0.5870.97
161_G165_L0.5840.97
180_A184_A0.5840.97
85_E89_G0.5820.97
128_F131_S0.5820.97
116_I167_A0.5790.97
159_Y163_I0.5750.97
43_I47_M0.5710.97
150_L153_A0.5700.97
66_L70_L0.5700.97
40_T137_A0.5660.96
100_T145_L0.5630.96
135_G138_S0.5610.96
50_K180_A0.5600.96
75_D78_E0.5580.96
137_A197_L0.5580.96
56_I219_V0.5550.96
154_I157_L0.5510.96
54_I57_V0.5510.96
106_C156_V0.5500.96
14_S147_I0.5500.96
182_M215_A0.5410.95
42_L57_V0.5410.95
12_V15_L0.5400.95
63_L218_L0.5390.95
40_T190_I0.5320.95
71_L75_D0.5320.95
10_I155_P0.5300.95
121_T126_L0.5270.95
3_T127_L0.5250.95
105_F149_V0.5210.94
1_L118_D0.5200.94
76_R83_W0.5190.94
47_M110_M0.5190.94
7_A11_L0.5180.94
179_I182_M0.5180.94
77_L99_V0.5180.94
38_L188_L0.5150.94
59_L132_V0.5130.94
14_S153_A0.5120.94
77_L107_V0.5110.94
178_M181_E0.5110.94
59_L214_I0.5110.94
153_A162_G0.5100.94
110_M193_I0.5090.94
112_I115_S0.5070.94
203_I208_L0.5070.94
51_T180_A0.5010.93
120_L167_A0.5000.93
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
1lypA 1 0.1455 6.4 0.962 Contact Map
1t5jA 1 0.2318 6.3 0.962 Contact Map
3rqwA 4 0.4545 4.8 0.964 Contact Map
2llyA 1 0.4955 4.6 0.965 Contact Map
4us3A 1 1 3.6 0.966 Contact Map
2nwlA 3 0.6273 3.2 0.967 Contact Map
3l1lA 2 0.3773 3 0.968 Contact Map
4eneA 2 0.4091 3 0.968 Contact Map
4o93B 1 0.7864 2.9 0.968 Contact Map
2pcoA 1 0.1136 2.8 0.968 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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