GREMLIN Database
DUF523 - Protein of unknown function (DUF523)
PFAM: PF04463 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 137 (134)
Sequences: 14481 (10624)
Seq/√Len: 917.8
META: 0.843

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
118_R126_M3.5281.00
43_V49_T2.8741.00
11_N18_H2.7501.00
52_E64_T2.3921.00
102_M126_M2.3721.00
123_A127_E2.3611.00
5_A10_E2.3201.00
59_R67_D2.0891.00
118_R123_A2.0561.00
124_L127_E1.9931.00
54_A68_V1.9581.00
93_L122_A1.9481.00
52_E63_K1.8941.00
103_E118_R1.8931.00
13_R19_K1.8391.00
45_G71_A1.8261.00
104_R116_K1.8041.00
52_E62_T1.6741.00
20_R25_T1.6731.00
84_K128_R1.6421.00
77_E124_L1.6371.00
2_G91_A1.6281.00
10_E38_P1.6261.00
90_G131_L1.6251.00
90_G133_P1.6011.00
32_V36_F1.5601.00
47_L68_V1.5551.00
62_T66_R1.5351.00
126_M134_V1.4621.00
95_S134_V1.4461.00
68_V71_A1.4311.00
74_R78_E1.4181.00
66_R71_A1.4031.00
124_L128_R1.4021.00
94_K135_E1.3701.00
58_P123_A1.3111.00
96_R114_L1.2931.00
44_E79_R1.2801.00
56_L123_A1.2681.00
52_E61_T1.2671.00
78_E81_E1.2361.00
79_R121_F1.2331.00
8_L38_P1.2321.00
81_E128_R1.2301.00
8_L36_F1.2211.00
96_R104_R1.2201.00
101_G105_V1.2001.00
45_G75_G1.1951.00
70_E74_R1.1951.00
39_V44_E1.1801.00
76_A124_L1.1451.00
91_A130_P1.1391.00
78_E82_E1.1391.00
107_D111_S1.1301.00
73_L124_L1.1291.00
80_L128_R1.1231.00
93_L125_L1.1201.00
53_P62_T1.1201.00
53_P63_K1.1121.00
61_T66_R1.1091.00
95_S103_E1.1071.00
2_G83_L1.1031.00
45_G54_A1.0981.00
81_E84_K1.0931.00
107_D110_F1.0791.00
54_A62_T1.0751.00
25_T29_G1.0701.00
54_A72_M1.0671.00
125_L132_L1.0611.00
22_R26_D1.0371.00
61_T68_V1.0371.00
2_G88_L1.0361.00
29_G32_V1.0301.00
79_R82_E1.0271.00
73_L77_E1.0231.00
54_A60_V1.0131.00
95_S136_E1.0071.00
47_L54_A1.0011.00
21_D24_L0.9971.00
102_M134_V0.9931.00
61_T65_G0.9921.00
73_L120_V0.9911.00
81_E85_E0.9901.00
61_T64_T0.9881.00
72_M99_S0.9861.00
80_L124_L0.9701.00
3_V36_F0.9631.00
102_M132_L0.9531.00
56_L120_V0.9431.00
75_G79_R0.9351.00
20_R29_G0.9201.00
107_D112_G0.9171.00
15_D19_K0.9111.00
20_R26_D0.9061.00
79_R125_L0.8901.00
45_G72_M0.8891.00
31_L36_F0.8801.00
107_D113_P0.8691.00
39_V75_G0.8481.00
23_F27_T0.8441.00
88_L91_A0.8441.00
43_V48_G0.8431.00
5_A38_P0.8421.00
12_V43_V0.8371.00
58_P120_V0.8371.00
95_S102_M0.8351.00
39_V79_R0.8321.00
56_L60_V0.8311.00
99_S121_F0.8281.00
72_M75_G0.8191.00
62_T65_G0.8141.00
105_V118_R0.8131.00
73_L123_A0.8101.00
5_A12_V0.8051.00
102_M118_R0.7951.00
76_A80_L0.7821.00
27_T30_R0.7701.00
45_G68_V0.7621.00
96_R136_E0.7621.00
55_R106_Y0.7621.00
5_A44_E0.7621.00
69_T73_L0.7591.00
60_V65_G0.7591.00
104_R114_L0.7581.00
2_G39_V0.7571.00
79_R83_L0.7361.00
23_F32_V0.7341.00
83_L88_L0.7331.00
96_R115_R0.7251.00
44_E75_G0.7171.00
82_E86_E0.7141.00
77_E81_E0.7111.00
118_R122_A0.7051.00
47_L71_A0.7021.00
44_E78_E0.6991.00
91_A121_F0.6991.00
83_L130_P0.6901.00
122_A126_M0.6881.00
52_E66_R0.6871.00
39_V83_L0.6841.00
68_V72_M0.6821.00
58_P73_L0.6741.00
60_V64_T0.6681.00
28_L31_L0.6661.00
93_L134_V0.6631.00
93_L102_M0.6621.00
3_V9_G0.6621.00
93_L100_C0.6611.00
105_V117_G0.6591.00
2_G125_L0.6541.00
54_A61_T0.6501.00
80_L129_G0.6461.00
74_R77_E0.6421.00
26_D30_R0.6421.00
8_L25_T0.6301.00
14_Y53_P0.6291.00
57_G73_L0.6241.00
104_R136_E0.6231.00
10_E43_V0.6201.00
14_Y17_G0.6151.00
11_N17_G0.6141.00
82_E85_E0.6091.00
24_L36_F0.6061.00
71_A74_R0.6031.00
126_M132_L0.6001.00
115_R118_R0.5901.00
89_D131_L0.5901.00
22_R25_T0.5901.00
95_S104_R0.5881.00
59_R70_E0.5851.00
62_T68_V0.5831.00
75_G80_L0.5781.00
55_R108_G0.5771.00
44_E82_E0.5741.00
39_V121_F0.5701.00
60_V63_K0.5521.00
43_V46_G0.5481.00
107_D114_L0.5441.00
87_G90_G0.5441.00
103_E136_E0.5431.00
9_G18_H0.5381.00
121_F125_L0.5361.00
29_G36_F0.5321.00
27_T31_L0.5291.00
84_K87_G0.5261.00
11_N20_R0.5241.00
24_L92_I0.5241.00
3_V8_L0.5221.00
118_R127_E0.5191.00
5_A9_G0.5191.00
93_L121_F0.5171.00
109_T113_P0.5141.00
28_L32_V0.5101.00
125_L134_V0.5101.00
103_E134_V0.5081.00
24_L27_T0.5071.00
24_L32_V0.5061.00
55_R110_F0.5051.00
31_L35_E0.5021.00
28_L36_F0.5001.00
77_E128_R0.5001.00
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
3tm8A 1 0.5693 8 0.937 Contact Map
4g7wA 1 0.3577 5.5 0.941 Contact Map
3pfnA 4 0.6204 4.7 0.943 Contact Map
3s8pA 1 0.8102 4.4 0.944 Contact Map
4g7xA 1 0.3212 4.3 0.944 Contact Map
2kswA 1 0.3577 3.7 0.946 Contact Map
4l6rA 1 0.2482 3.3 0.947 Contact Map
1u6tA 1 0.3212 3.1 0.947 Contact Map
2yynA 2 0.3066 2.4 0.95 Contact Map
2p2eA 1 0.5328 2.4 0.951 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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