GREMLIN Database
DUF512 - Protein of unknown function (DUF512)
PFAM: PF04459 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 198 (197)
Sequences: 6954 (5255)
Seq/√Len: 374.4
META: 0.903

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
63_A69_F3.0051.00
38_R44_R2.7161.00
98_T128_V2.6981.00
28_D58_R2.5191.00
16_R22_E2.5181.00
19_T22_E2.4281.00
43_T67_E2.3241.00
133_D152_A2.3231.00
63_A84_D2.3121.00
24_R58_R2.2741.00
44_R67_E2.2131.00
20_P57_G2.2011.00
134_F146_T2.1201.00
106_A129_P2.0781.00
131_E137_E2.0551.00
130_V153_A2.0421.00
96_R124_D2.0411.00
96_R126_R1.9841.00
170_L177_F1.9291.00
103_T131_E1.9011.00
87_E115_R1.8711.00
151_I181_M1.8701.00
36_R40_E1.8321.00
145_L169_M1.8201.00
27_I48_A1.8161.00
60_L80_R1.8101.00
110_R114_E1.7981.00
47_Y67_E1.7821.00
30_V48_A1.7461.00
53_Y65_H1.7201.00
151_I186_L1.7161.00
103_T129_P1.6881.00
133_D137_E1.6741.00
98_T160_G1.6701.00
106_A110_R1.6701.00
18_V22_E1.6451.00
113_L127_V1.6401.00
100_V150_I1.6281.00
31_E58_R1.5861.00
60_L79_V1.5841.00
142_A168_V1.5751.00
79_V108_V1.5671.00
147_G181_M1.5501.00
96_R161_D1.5441.00
162_R195_R1.5221.00
80_R83_L1.4931.00
150_I169_M1.4841.00
101_T142_A1.4801.00
23_A55_L1.4691.00
177_F186_L1.4661.00
169_M177_F1.4661.00
181_M185_D1.4151.00
105_A109_L1.4141.00
25_A29_Q1.3911.00
9_R12_L1.3751.00
71_Q74_N1.3751.00
151_I189_A1.3701.00
86_F112_L1.3661.00
29_Q33_W1.3371.00
24_R28_D1.3341.00
126_R157_K1.3341.00
148_Q152_A1.3301.00
170_L183_L1.3211.00
7_R137_E1.3201.00
187_E193_P1.3191.00
17_P138_T1.3131.00
20_P24_R1.3101.00
26_V52_F1.3051.00
34_Q65_H1.2981.00
80_R84_D1.2871.00
78_M168_V1.2801.00
63_A80_R1.2771.00
142_A166_P1.2641.00
41_L45_F1.2601.00
171_R174_E1.2361.00
185_D188_R1.2311.00
83_L111_P1.2281.00
155_K189_A1.1851.00
164_L197_V1.1831.00
72_L81_L1.1811.00
171_R176_V1.1741.00
79_V105_A1.1681.00
175_G184_E1.1681.00
105_A142_A1.1661.00
165_L196_V1.1611.00
146_T178_L1.1601.00
101_T105_A1.1581.00
69_F80_R1.1531.00
95_R162_R1.1411.00
33_W37_F1.1371.00
131_E153_A1.1281.00
135_F146_T1.1271.00
114_E117_N1.1231.00
103_T137_E1.1231.00
62_P65_H1.1191.00
183_L187_E1.1191.00
96_R158_D1.1041.00
97_V162_R1.1011.00
48_A52_F1.1001.00
27_I58_R1.1001.00
128_V157_K1.0891.00
54_L108_V1.0791.00
18_V52_F1.0691.00
86_F116_L1.0601.00
104_L140_T1.0541.00
83_L108_V1.0441.00
26_V29_Q1.0441.00
34_Q44_R1.0441.00
163_L192_V1.0421.00
133_D153_A1.0421.00
113_L117_N1.0311.00
31_E65_H1.0291.00
81_L85_E1.0291.00
97_V125_V1.0231.00
132_N135_F1.0221.00
44_R65_H1.0171.00
137_E140_T1.0161.00
182_T185_D1.0151.00
100_V163_L1.0141.00
22_E26_V1.0111.00
72_L78_M1.0061.00
33_W36_R0.9991.00
183_L196_V0.9811.00
98_T159_L0.9721.00
82_F112_L0.9691.00
162_R193_P0.9651.00
186_L194_V0.9581.00
31_E34_Q0.9571.00
185_D189_A0.9551.00
171_R175_G0.9461.00
37_F46_V0.9421.00
108_V111_P0.9321.00
27_I31_E0.9231.00
82_F105_A0.9091.00
184_E188_R0.9051.00
21_E24_R0.9011.00
31_E53_Y0.8961.00
151_I155_K0.8801.00
83_L87_E0.8731.00
22_E25_A0.8711.00
176_V180_D0.8681.00
105_A108_V0.8671.00
83_L112_L0.8641.00
38_R67_E0.8551.00
54_L79_V0.8491.00
33_W46_V0.8471.00
3_V15_L0.8401.00
98_T126_R0.8341.00
90_L115_R0.8311.00
170_L196_V0.8241.00
95_R161_D0.8191.00
176_V182_T0.8131.00
86_F90_L0.8051.00
31_E35_K0.8001.00
100_V130_V0.7911.00
184_E187_E0.7891.00
18_V26_V0.7861.00
163_L190_L0.7851.00
48_A53_Y0.7831.00
29_Q32_R0.7791.00
172_N175_G0.7781.00
165_L170_L0.7611.00
99_I164_L0.7591.00
57_G107_P0.7591.00
55_L58_R0.7561.00
21_E25_A0.7451.00
69_F84_D0.7441.00
100_V154_L0.7421.00
109_L127_V0.7301.00
128_V163_L0.7271.00
79_V83_L0.7251.00
188_R191_G0.7211.00
24_R56_A0.7201.00
86_F115_R0.7141.00
175_G183_L0.7131.00
150_I186_L0.7070.99
47_Y70_P0.7070.99
88_E92_R0.7050.99
173_G176_V0.7010.99
37_F45_F0.6980.99
113_L125_V0.6980.99
9_R14_P0.6960.99
120_I123_L0.6930.99
102_G129_P0.6900.99
98_T163_L0.6880.99
98_T161_D0.6880.99
49_A52_F0.6860.99
128_V153_A0.6810.99
129_P145_L0.6810.99
165_L169_M0.6800.99
130_V145_L0.6790.99
103_T140_T0.6760.99
27_I56_A0.6730.99
112_L115_R0.6710.99
37_F41_L0.6660.99
25_A28_D0.6640.99
35_K39_K0.6640.99
117_N124_D0.6610.99
43_T47_Y0.6600.99
32_R36_R0.6600.99
7_R133_D0.6590.99
44_R47_Y0.6560.99
54_L104_L0.6510.99
107_P111_P0.6490.99
69_F72_L0.6490.99
48_A65_H0.6480.99
34_Q38_R0.6480.99
89_A93_L0.6480.99
84_D87_E0.6430.99
114_E118_N0.6390.99
134_F148_Q0.6370.99
97_V123_L0.6330.99
170_L175_G0.6300.99
47_Y74_N0.6280.99
100_V166_P0.6240.99
93_L120_I0.6230.99
5_L15_L0.6230.99
26_V30_V0.6220.99
54_L60_L0.6200.99
107_P110_R0.6190.99
145_L153_A0.6130.99
49_A76_V0.6110.99
154_L159_L0.6100.99
165_L177_F0.6090.98
145_L150_I0.6080.98
97_V164_L0.6070.98
99_I113_L0.6050.98
116_L123_L0.6000.98
152_A155_K0.5990.98
106_A127_V0.5910.98
16_R19_T0.5880.98
175_G182_T0.5880.98
102_G105_A0.5880.98
55_L104_L0.5850.98
64_E88_E0.5840.98
103_T106_A0.5830.98
172_N176_V0.5820.98
84_D88_E0.5790.98
117_N123_L0.5750.98
111_P115_R0.5710.98
117_N120_I0.5710.98
186_L190_L0.5710.98
88_E91_R0.5710.98
94_P124_D0.5690.98
94_P120_I0.5680.98
30_V34_Q0.5680.98
94_P122_G0.5660.98
5_L138_T0.5660.98
85_E89_A0.5650.98
63_A68_D0.5640.98
142_A169_M0.5630.98
86_F89_A0.5600.97
7_R138_T0.5560.97
34_Q48_A0.5500.97
17_P22_E0.5500.97
10_E13_P0.5490.97
145_L166_P0.5450.97
23_A26_V0.5410.97
38_R41_L0.5410.97
95_R123_L0.5400.97
126_R161_D0.5390.97
35_K65_H0.5390.97
153_A156_G0.5390.97
115_R118_N0.5330.97
49_A104_L0.5330.97
175_G187_E0.5270.96
89_A92_R0.5270.96
163_L194_V0.5260.96
60_L65_H0.5260.96
26_V33_W0.5250.96
111_P114_E0.5240.96
157_K163_L0.5240.96
73_E78_M0.5240.96
85_E88_E0.5230.96
9_R15_L0.5220.96
23_A56_A0.5220.96
151_I190_L0.5200.96
154_L163_L0.5180.96
34_Q46_V0.5170.96
110_R129_P0.5170.96
148_Q189_A0.5160.96
94_P121_P0.5160.96
181_M186_L0.5160.96
5_L17_P0.5150.96
115_R119_K0.5140.96
90_L93_L0.5130.96
38_R43_T0.5100.96
59_P62_P0.5080.95
3_V18_V0.5080.95
72_L139_V0.5070.95
15_L18_V0.5060.95
147_G177_F0.5050.95
35_K62_P0.5010.95
90_L120_I0.5010.95
100_V142_A0.5010.95
159_L192_V0.5000.95
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2qu7A 2 0.4293 38.1 0.951 Contact Map
3cs3A 2 0.4242 34.2 0.952 Contact Map
3c3kA 2 0.4394 31.8 0.953 Contact Map
1dbqA 2 0.4394 28.6 0.954 Contact Map
3cijA 1 0.4394 25.4 0.955 Contact Map
3cfxA 1 0.4444 25 0.955 Contact Map
3egcA 2 0.4444 24.6 0.955 Contact Map
3o8oB 1 0.4899 23.7 0.956 Contact Map
1atgA 1 0.4242 23.1 0.956 Contact Map
3clkA 2 0.4141 20.4 0.957 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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