GREMLIN Database
DUF544 - Protein of unknown function (DUF544)
PFAM: PF04424 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 117 (116)
Sequences: 568 (435)
Seq/√Len: 40.4
META: 0.255

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
110_N113_V3.0711.00
97_D100_T3.0131.00
58_G62_N2.4051.00
104_V112_L2.4011.00
106_A112_L2.3701.00
2_N6_L2.3451.00
73_Y81_D2.2121.00
58_G91_W2.1840.99
72_E110_N2.1750.99
68_V88_V2.1400.99
47_D72_E2.0860.99
22_L56_H1.9870.99
26_L55_L1.9570.99
100_T103_A1.9070.99
62_N72_E1.9020.98
74_T81_D1.8220.98
54_K76_E1.8040.98
66_T70_D1.7710.98
111_Q114_E1.7280.97
49_L53_P1.6210.96
51_F80_F1.5690.95
69_D86_P1.5600.95
61_V85_I1.5360.94
97_D103_A1.5300.94
17_R33_L1.5160.94
1_C9_K1.5130.94
99_E102_A1.4900.93
17_R24_L1.4740.93
20_V25_L1.4380.92
79_L93_V1.4090.91
55_L83_L1.4070.91
41_Y44_N1.3880.91
101_A105_G1.3830.91
29_L83_L1.3740.90
101_A104_V1.3710.90
47_D62_N1.3010.88
21_S29_L1.2980.88
107_K115_L1.2580.86
62_N91_W1.2420.85
61_V108_S1.2350.85
72_E81_D1.2110.84
29_L85_I1.2050.83
66_T92_L1.1790.82
29_L57_T1.1720.82
62_N81_D1.1720.82
70_D116_L1.1610.81
37_N41_Y1.1610.81
14_P22_L1.1360.79
37_N84_G1.1360.79
52_L82_L1.1280.79
29_L52_L1.1130.78
56_H63_P1.0940.77
53_P59_L1.0930.77
30_A52_L1.0880.76
51_F78_A1.0880.76
67_G70_D1.0730.75
48_A63_P1.0550.74
79_L83_L1.0540.74
63_P115_L1.0510.74
81_D110_N1.0490.74
105_G112_L1.0440.73
24_L33_L1.0370.73
59_L63_P1.0260.72
25_L47_D1.0230.72
83_L112_L1.0130.71
21_S33_L1.0110.71
58_G110_N0.9990.70
60_D113_V0.9920.69
62_N109_Y0.9850.69
20_V24_L0.9730.68
48_A82_L0.9610.67
93_V101_A0.9530.66
40_N44_N0.9300.64
55_L61_V0.9220.64
5_L17_R0.9200.64
102_A107_K0.9160.63
107_K111_Q0.9150.63
25_L95_P0.9110.63
40_N43_Q0.8970.62
5_L13_L0.8780.60
113_V116_L0.8690.59
69_D92_L0.8660.59
34_L37_N0.8630.59
97_D102_A0.8580.58
67_G106_A0.8540.58
60_D73_Y0.8520.58
36_S65_F0.8440.57
61_V87_L0.8330.56
21_S25_L0.8210.55
31_D46_S0.8160.54
43_Q85_I0.8110.54
70_D105_G0.8100.54
15_P45_I0.7800.51
60_D72_E0.7760.51
5_L23_E0.7680.50
80_F94_D0.7660.50
78_A81_D0.7630.49
7_R12_L0.7550.49
59_L68_V0.7430.48
81_D112_L0.7430.48
38_P92_L0.7420.47
60_D110_N0.7400.47
110_N114_E0.7280.46
64_R74_T0.7130.45
13_L52_L0.7110.45
98_A104_V0.7080.44
29_L49_L0.7040.44
5_L12_L0.7030.44
22_L26_L0.7010.44
18_E74_T0.7000.44
55_L108_S0.6980.43
66_T111_Q0.6980.43
16_D63_P0.6950.43
5_L10_I0.6930.43
57_T69_D0.6930.43
44_N72_E0.6820.42
44_N57_T0.6770.42
53_P80_F0.6750.41
31_D60_D0.6710.41
57_T71_F0.6600.40
48_A51_F0.6600.40
29_L64_R0.6570.40
38_P109_Y0.6530.39
22_L83_L0.6500.39
60_D65_F0.6500.39
70_D77_L0.6480.39
73_Y110_N0.6420.38
85_I106_A0.6410.38
42_E84_G0.6340.38
5_L21_S0.6300.37
30_A49_L0.6230.37
60_D81_D0.6190.37
3_V7_R0.6160.36
100_T106_A0.6150.36
92_L95_P0.6040.35
66_T93_V0.6010.35
70_D88_V0.6000.35
27_E30_A0.5990.35
24_L112_L0.5990.35
96_Q105_G0.5890.34
48_A113_V0.5880.34
49_L54_K0.5870.34
58_G74_T0.5860.34
105_G111_Q0.5840.34
98_A102_A0.5800.33
99_E104_V0.5750.33
18_E21_S0.5750.33
71_F75_P0.5660.32
44_N88_V0.5600.32
35_S86_P0.5570.31
21_S87_L0.5550.31
51_F56_H0.5520.31
59_L80_F0.5500.31
37_N70_D0.5440.30
102_A105_G0.5440.30
83_L105_G0.5380.30
9_K88_V0.5350.30
56_H84_G0.5340.30
12_L93_V0.5340.30
51_F55_L0.5300.29
19_Q60_D0.5260.29
47_D55_L0.5240.29
45_I106_A0.5220.29
46_S73_Y0.5210.29
20_V98_A0.5210.29
60_D76_E0.5190.28
29_L46_S0.5160.28
98_A101_A0.5130.28
1_C10_I0.5120.28
24_L31_D0.5070.28
83_L104_V0.5050.27
45_I96_Q0.5020.27
65_F93_V0.5000.27
35_S38_P0.5000.27
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2ynaA 2 0.735 16.2 0.918 Contact Map
3d23B 4 0.7094 14 0.92 Contact Map
1lvoA 5 0.7265 6.3 0.932 Contact Map
3tloA 2 0.7265 6.3 0.932 Contact Map
3kzpA 2 0.8205 5.7 0.933 Contact Map
1v95A 1 0.4872 5.3 0.934 Contact Map
2mn2A 1 0.3419 5.1 0.935 Contact Map
3tntA 2 0.7265 4.8 0.936 Contact Map
4rniA 2 0.8974 4.5 0.936 Contact Map
3rznA 2 0.8974 3.9 0.938 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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