GREMLIN Database
DUF535 - Protein of unknown function (DUF535)
PFAM: PF04393 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 281 (275)
Sequences: 1576 (1162)
Seq/√Len: 70.1
META: 0.626

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
263_A267_R7.0401.00
251_R273_D5.3281.00
150_L191_A4.0701.00
204_N225_D3.7571.00
205_A227_D3.6421.00
198_R244_E3.5691.00
74_F141_L3.5551.00
108_L186_A3.5441.00
178_R232_E3.4911.00
60_S63_Q3.4271.00
253_P256_E3.2661.00
150_L187_L3.1731.00
225_D228_E3.0061.00
54_Y121_E2.8371.00
270_A274_E2.5871.00
231_E243_F2.5541.00
138_T154_G2.5361.00
263_A266_R2.4951.00
147_E197_E2.3651.00
72_Y121_E2.3231.00
260_K263_A2.3071.00
261_K265_Y2.2711.00
184_L233_L2.2411.00
102_D173_A2.0361.00
152_I187_L2.0051.00
261_K264_E2.0011.00
175_H267_R1.9981.00
94_V97_E1.9591.00
265_Y269_Y1.9561.00
170_A182_L1.9491.00
80_P83_L1.8911.00
264_E269_Y1.8331.00
206_G241_G1.8221.00
123_T136_S1.8131.00
274_E278_Q1.7271.00
74_F78_L1.7051.00
123_T138_T1.6971.00
94_V107_R1.6781.00
137_L187_L1.6751.00
97_E105_R1.6540.99
164_L168_R1.6530.99
94_V109_R1.6340.99
188_R249_L1.6080.99
265_Y272_L1.6070.99
92_G109_R1.5950.99
55_L67_A1.5900.99
261_K269_Y1.5810.99
261_K272_L1.5650.99
21_R25_R1.5420.99
67_A142_A1.5280.99
49_K121_E1.5150.99
184_L229_F1.5140.99
178_R181_N1.5130.99
259_S264_E1.5100.99
231_E237_P1.5020.99
26_W65_L1.4890.99
269_Y272_L1.4760.99
238_R244_E1.4740.99
71_H122_L1.4520.99
139_F187_L1.4410.99
265_Y268_R1.4380.99
207_H213_R1.3990.98
55_L59_L1.3990.98
25_R69_I1.3830.98
22_A65_L1.3800.98
155_L229_F1.3720.98
180_K204_N1.3680.98
141_L194_L1.3570.98
192_R276_A1.3510.98
171_T176_G1.3350.98
201_A235_G1.3300.98
149_T200_L1.3270.97
20_P24_R1.3240.97
203_S227_D1.3030.97
29_F73_R1.3010.97
238_R241_G1.2970.97
269_Y273_D1.2710.97
259_S263_A1.2620.97
106_Y173_A1.2600.97
56_S242_F1.2580.97
181_N232_E1.2430.96
11_K15_R1.2430.96
106_Y128_D1.2390.96
271_L275_L1.2370.96
23_T27_L1.2320.96
181_N233_L1.2300.96
168_R172_K1.2260.96
109_R127_R1.2100.96
54_Y72_Y1.2040.96
230_W235_G1.1980.95
236_V244_E1.1910.95
105_R129_G1.1890.95
251_R269_Y1.1670.95
107_R127_R1.1630.95
106_Y170_A1.1500.94
150_L196_V1.1500.94
204_N227_D1.1340.94
257_I265_Y1.1320.94
56_S241_G1.1310.94
207_H214_Y1.1290.94
36_L76_E1.1230.93
118_R202_V1.1230.93
274_E277_A1.1200.93
158_P167_I1.1190.93
17_L21_R1.1130.93
200_L238_R1.1100.93
74_F194_L1.1060.93
152_I201_A1.0960.93
128_D132_E1.0930.93
95_L124_L1.0920.93
70_D143_P1.0900.92
50_L65_L1.0780.92
55_L140_S1.0730.92
227_D231_E1.0720.92
268_R272_L1.0700.92
180_K224_A1.0690.92
13_L24_R1.0630.91
38_A85_R1.0590.91
9_R13_L1.0560.91
252_K261_K1.0520.91
187_L191_A1.0480.91
98_L275_L1.0420.91
153_G230_W1.0310.90
125_S133_R1.0280.90
71_H140_S1.0200.90
176_G261_K1.0200.90
118_R209_Y1.0180.90
124_L139_F1.0120.89
124_L190_L1.0060.89
254_L257_I1.0050.89
126_L183_L1.0030.89
211_A214_Y1.0010.89
66_A70_D0.9950.89
264_E272_L0.9910.88
55_L242_F0.9900.88
260_K265_Y0.9790.88
206_G242_F0.9790.88
167_I171_T0.9790.88
116_F138_T0.9620.87
24_R28_R0.9610.87
108_L126_L0.9570.87
133_R136_S0.9570.87
111_D125_S0.9550.86
56_S59_L0.9520.86
165_E169_E0.9510.86
171_T179_P0.9490.86
260_K264_E0.9480.86
87_L255_E0.9450.86
116_F136_S0.9430.86
12_F15_R0.9410.86
38_A41_A0.9380.85
264_E271_L0.9370.85
195_G198_R0.9350.85
95_L199_I0.9340.85
252_K265_Y0.9300.85
104_E129_G0.9300.85
254_L260_K0.9280.85
6_L13_L0.9200.84
48_E52_R0.9190.84
101_K175_H0.9160.84
167_I182_L0.9150.84
20_P25_R0.9080.83
151_F200_L0.9000.83
21_R32_S0.8940.83
38_A42_A0.8870.82
143_P146_G0.8700.81
48_E118_R0.8650.80
256_E275_L0.8620.80
135_Y171_T0.8520.79
21_R28_R0.8460.79
151_F242_F0.8460.79
58_G241_G0.8460.79
154_G204_N0.8430.79
127_R133_R0.8410.79
155_L201_A0.8320.78
265_Y273_D0.8270.77
253_P269_Y0.8270.77
165_E168_R0.8220.77
173_A202_V0.8160.76
134_L166_L0.8150.76
59_L113_P0.8150.76
209_Y213_R0.8130.76
178_R264_E0.8120.76
235_G246_P0.8030.75
207_H211_A0.7990.75
33_S76_E0.7930.74
235_G245_L0.7930.74
65_L69_I0.7900.74
75_L122_L0.7890.74
68_L230_W0.7860.74
40_L47_L0.7800.73
104_E173_A0.7790.73
203_S206_G0.7780.73
166_L169_E0.7760.73
126_L174_C0.7760.73
194_L243_F0.7720.72
23_T30_L0.7700.72
227_D243_F0.7700.72
51_H178_R0.7670.72
40_L44_P0.7660.72
158_P222_L0.7640.72
87_L112_A0.7630.71
133_R166_L0.7570.71
52_R118_R0.7560.71
162_D166_L0.7560.71
15_R201_A0.7540.71
17_L20_P0.7530.70
253_P258_P0.7520.70
137_L155_L0.7520.70
137_L154_G0.7510.70
27_L30_L0.7500.70
20_P23_T0.7500.70
12_F26_W0.7490.70
72_Y203_S0.7490.70
181_N249_L0.7490.70
176_G268_R0.7480.70
142_A151_F0.7430.69
251_R265_Y0.7430.69
8_R11_K0.7430.69
75_L79_F0.7410.69
96_A278_Q0.7400.69
63_Q67_A0.7350.69
29_F229_F0.7340.68
257_I269_Y0.7320.68
21_R41_A0.7290.68
14_L18_L0.7280.68
172_K180_K0.7280.68
28_R34_P0.7250.68
73_R143_P0.7250.68
235_G247_L0.7250.68
190_L279_I0.7230.67
188_R199_I0.7230.67
45_R186_A0.7210.67
59_L67_A0.7160.67
178_R252_K0.7110.66
68_L86_A0.7110.66
49_K112_A0.7070.66
75_L84_L0.7040.65
141_L183_L0.7030.65
135_Y183_L0.7030.65
261_K268_R0.7030.65
222_L252_K0.7010.65
158_P180_K0.7000.65
110_L122_L0.6920.64
191_A196_V0.6910.64
6_L14_L0.6900.64
108_L183_L0.6900.64
101_K259_S0.6860.63
152_I176_G0.6830.63
49_K52_R0.6810.63
240_D244_E0.6810.63
101_K264_E0.6790.62
177_L182_L0.6760.62
184_L187_L0.6760.62
257_I260_K0.6730.62
254_L266_R0.6720.62
75_L141_L0.6720.62
258_P263_A0.6670.61
121_E140_S0.6670.61
171_T204_N0.6650.61
245_L257_I0.6610.60
130_D195_G0.6550.60
115_G133_R0.6540.60
79_F229_F0.6530.59
138_T179_P0.6520.59
62_R66_A0.6510.59
177_L272_L0.6490.59
52_R261_K0.6480.59
171_T178_R0.6450.59
141_L176_G0.6430.58
208_V211_A0.6420.58
61_A64_R0.6410.58
182_L275_L0.6390.58
147_E236_V0.6390.58
23_T51_H0.6380.58
98_L193_A0.6360.57
157_G171_T0.6360.57
255_E273_D0.6340.57
12_F16_S0.6320.57
68_L216_L0.6310.57
139_F156_Q0.6300.57
23_T50_L0.6280.56
22_A25_R0.6270.56
252_K259_S0.6270.56
13_L16_S0.6260.56
122_L221_K0.6220.56
62_R195_G0.6200.56
16_S20_P0.6190.55
7_R10_L0.6190.55
201_A230_W0.6190.55
70_D142_A0.6170.55
181_N185_E0.6160.55
73_R77_Q0.6160.55
140_S151_F0.6150.55
15_R55_L0.6150.55
49_K72_Y0.6130.55
103_G255_E0.6120.55
236_V278_Q0.6110.54
262_R268_R0.6100.54
135_Y180_K0.6100.54
79_F87_L0.6090.54
16_S19_H0.6070.54
147_E198_R0.6050.54
196_V247_L0.6040.54
59_L242_F0.6020.53
111_D115_G0.5990.53
248_P277_A0.5980.53
249_L253_P0.5980.53
203_S237_P0.5970.53
8_R12_F0.5950.53
49_K54_Y0.5950.53
46_L83_L0.5950.53
59_L125_S0.5950.53
115_G208_V0.5940.52
153_G180_K0.5940.52
167_I170_A0.5920.52
189_A276_A0.5920.52
276_A279_I0.5920.52
127_R130_D0.5910.52
41_A190_L0.5900.52
108_L115_G0.5900.52
6_L9_R0.5890.52
32_S36_L0.5850.51
205_A237_P0.5840.51
186_A201_A0.5840.51
82_A94_V0.5820.51
189_A269_Y0.5810.51
271_L279_I0.5800.51
257_I261_K0.5790.51
67_A204_N0.5780.50
66_A192_R0.5780.50
74_F196_V0.5770.50
28_R38_A0.5770.50
108_L276_A0.5770.50
141_L148_R0.5760.50
261_K267_R0.5750.50
109_R243_F0.5720.50
120_G156_Q0.5720.50
202_V208_V0.5720.50
158_P188_R0.5700.49
172_K176_G0.5690.49
56_S121_E0.5690.49
188_R248_P0.5680.49
119_E156_Q0.5680.49
203_S241_G0.5650.49
29_F69_I0.5630.49
254_L263_A0.5630.49
10_L14_L0.5620.49
185_E188_R0.5610.48
19_H37_R0.5610.48
115_G209_Y0.5600.48
71_H243_F0.5590.48
170_A173_A0.5580.48
81_P279_I0.5580.48
127_R132_E0.5560.48
129_G222_L0.5560.48
178_R261_K0.5560.48
164_L167_I0.5550.48
56_S201_A0.5540.48
99_S105_R0.5530.47
43_Q46_L0.5530.47
92_G127_R0.5530.47
165_E271_L0.5520.47
213_R216_L0.5500.47
66_A77_Q0.5470.47
115_G138_T0.5470.47
40_L223_K0.5460.47
28_R97_E0.5460.47
257_I263_A0.5460.47
207_H212_L0.5450.46
134_L160_G0.5440.46
181_N229_F0.5430.46
98_L108_L0.5420.46
68_L71_H0.5420.46
102_D169_E0.5410.46
77_Q277_A0.5410.46
153_G196_V0.5390.46
135_Y181_N0.5370.45
177_L249_L0.5360.45
17_L236_V0.5330.45
176_G180_K0.5320.45
193_A248_P0.5310.45
73_R145_D0.5280.44
118_R226_Y0.5270.44
68_L134_L0.5220.44
152_I199_I0.5220.44
155_L167_I0.5200.43
7_R255_E0.5200.43
191_A247_L0.5190.43
171_T261_K0.5190.43
35_L38_A0.5190.43
80_P224_A0.5190.43
119_E139_F0.5190.43
153_G202_V0.5190.43
235_G268_R0.5180.43
140_S210_R0.5150.43
7_R107_R0.5130.43
191_A199_I0.5130.43
178_R214_Y0.5130.43
124_L226_Y0.5120.43
153_G203_S0.5120.43
270_A275_L0.5100.42
80_P84_L0.5100.42
23_T26_W0.5100.42
13_L201_A0.5100.42
266_R270_A0.5100.42
56_S206_G0.5070.42
107_R152_I0.5050.42
167_I180_K0.5040.42
139_F202_V0.5040.42
38_A235_G0.5040.42
172_K211_A0.5000.41
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
4mh8A 1 0.1957 14.4 0.962 Contact Map
4xpcA 1 0.2028 12.6 0.963 Contact Map
1gyxA 2 0.2456 9.8 0.965 Contact Map
3bz1X 1 0.121 7.9 0.966 Contact Map
4b2uA 1 0.1281 6.2 0.968 Contact Map
1gd2E 2 0.1032 5.5 0.969 Contact Map
4q9vA 2 0.363 5.3 0.969 Contact Map
2m8vA 1 0.1068 4.6 0.97 Contact Map
3i9v4 1 0.5231 4.5 0.97 Contact Map
3tpfA 3 0.6548 4.3 0.97 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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