GREMLIN Database
DUF494 - Protein of unknown function (DUF494)
PFAM: PF04361 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 153 (151)
Sequences: 3922 (2326)
Seq/√Len: 189.3
META: 0.901

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
89_E99_R4.0501.00
94_L102_V3.8241.00
74_Q82_R3.7861.00
81_C119_L3.1871.00
69_Y82_R3.0851.00
106_A119_L2.8341.00
78_D81_C2.4181.00
78_D82_R2.1911.00
76_R107_M2.1591.00
71_D74_Q2.1591.00
85_L102_V2.1461.00
135_P139_W2.1281.00
5_L9_F1.9971.00
79_T83_G1.9841.00
119_L123_V1.9441.00
69_Y86_L1.8831.00
86_L90_Q1.8571.00
84_F116_L1.7991.00
105_R108_A1.7931.00
106_A122_I1.7571.00
89_E96_P1.6871.00
87_F91_A1.6641.00
137_Y145_F1.5921.00
111_G118_D1.5911.00
137_Y140_M1.5841.00
83_G90_Q1.5641.00
29_S38_I1.5361.00
148_E151_L1.5171.00
20_D23_E1.5121.00
132_G135_P1.4991.00
82_R103_I1.4871.00
15_P18_C1.4701.00
139_W142_D1.4661.00
95_D133_Q1.4581.00
115_D118_D1.4571.00
142_D145_F1.4041.00
131_P136_A1.3951.00
106_A114_I1.3841.00
141_E145_F1.3731.00
95_D98_Q1.3511.00
99_R103_I1.3321.00
51_A101_L1.3081.00
141_E144_V1.3001.00
79_T82_R1.2941.00
28_L38_I1.2931.00
77_L81_C1.2801.00
143_L150_R1.2761.00
47_G108_A1.2411.00
21_R25_A1.2401.00
27_E30_A1.2261.00
72_E107_M1.2251.00
1_M42_L1.2221.00
67_R73_E1.2171.00
93_V98_Q1.2171.00
69_Y99_R1.2121.00
26_R30_A1.1851.00
47_G105_R1.1821.00
69_Y89_E1.1731.00
28_L32_G1.1721.00
77_L106_A1.1691.00
76_R113_E1.1211.00
147_D150_R1.1151.00
87_F90_Q1.0971.00
136_A140_M1.0871.00
76_R110_D1.0791.00
37_E120_K1.0761.00
135_P138_L1.0681.00
49_A129_N1.0651.00
109_L121_W1.0541.00
33_F41_A1.0511.00
128_F138_L1.0461.00
77_L82_R1.0461.00
132_G137_Y1.0441.00
145_F149_P1.0411.00
84_F87_F1.0301.00
138_L143_L1.0261.00
134_E137_Y1.0171.00
74_Q79_T1.0171.00
133_Q143_L1.0121.00
138_L142_D1.0101.00
55_P97_A0.9820.99
85_L103_I0.9770.99
33_F125_M0.9730.99
55_P58_P0.9720.99
137_Y141_E0.9670.99
102_V123_V0.9590.99
124_L128_F0.9400.99
51_A55_P0.9320.99
139_W143_L0.9320.99
45_L100_E0.9260.99
55_P60_P0.9250.99
121_W124_L0.9230.99
138_L145_F0.9120.99
85_L99_R0.9080.99
144_V148_E0.9000.99
83_G86_L0.8940.99
145_F150_R0.8920.99
77_L119_L0.8890.99
102_V119_L0.8770.99
9_F125_M0.8690.99
34_E37_E0.8680.99
61_P95_D0.8550.98
40_K110_D0.8540.98
131_P134_E0.8520.98
101_L125_M0.8520.98
28_L33_F0.8510.98
46_D50_E0.8510.98
37_E105_R0.8500.98
114_I147_D0.8470.98
102_V122_I0.8460.98
69_Y103_I0.8360.98
17_A21_R0.8350.98
135_P141_E0.8350.98
144_V151_L0.8330.98
19_P24_L0.8250.98
98_Q130_Q0.8230.98
26_R29_S0.8150.98
63_S97_A0.8120.98
2_F97_A0.8100.98
127_L130_Q0.8070.98
140_M143_L0.8040.98
41_A105_R0.7850.97
129_N133_Q0.7800.97
85_L119_L0.7710.97
145_F148_E0.7630.97
104_D108_A0.7550.97
147_D151_L0.7530.96
89_E94_L0.7530.96
84_F88_L0.7510.96
57_A97_A0.7390.96
29_S151_L0.7380.96
125_M128_F0.7380.96
32_G38_I0.7370.96
108_A122_I0.7350.96
43_D47_G0.7300.96
57_A60_P0.7260.96
12_Y33_F0.7260.96
33_F38_I0.7260.96
133_Q145_F0.7230.96
9_F13_M0.7210.95
88_L123_V0.7190.95
136_A139_W0.7060.95
45_L48_L0.7040.95
10_E117_E0.7030.95
25_A29_S0.7000.95
146_D150_R0.6950.94
8_L124_L0.6950.94
21_R24_L0.6890.94
106_A150_R0.6840.94
134_E140_M0.6840.94
2_F5_L0.6830.94
139_W151_L0.6720.93
140_M145_F0.6650.93
101_L130_Q0.6630.93
55_P59_A0.6630.93
139_W144_V0.6630.93
7_Y100_E0.6610.93
122_I125_M0.6590.93
58_P75_E0.6590.93
2_F18_C0.6520.92
4_V33_F0.6500.92
13_M111_G0.6480.92
1_M128_F0.6470.92
109_L113_E0.6430.92
146_D151_L0.6370.92
132_G140_M0.6360.91
139_W150_R0.6360.91
5_L96_P0.6340.91
146_D149_P0.6290.91
12_Y27_E0.6260.91
138_L141_E0.6210.90
8_L127_L0.6210.90
6_V9_F0.6200.90
123_V133_Q0.6160.90
39_S43_D0.6080.90
1_M118_D0.6070.89
136_A150_R0.5960.89
137_Y147_D0.5900.88
48_L88_L0.5860.88
135_P140_M0.5850.88
31_A70_S0.5800.87
7_Y27_E0.5770.87
44_W105_R0.5760.87
136_A145_F0.5740.87
1_M138_L0.5730.87
137_Y142_D0.5720.87
130_Q133_Q0.5680.86
56_A97_A0.5670.86
4_V8_L0.5660.86
98_Q129_N0.5660.86
3_D145_F0.5640.86
7_Y31_A0.5600.85
51_A54_S0.5600.85
71_D75_E0.5590.85
107_M112_D0.5590.85
22_D79_T0.5510.84
27_E31_A0.5480.84
10_E124_L0.5470.84
41_A44_W0.5470.84
54_S59_A0.5460.84
143_L151_L0.5460.84
139_W145_F0.5450.84
135_P149_P0.5430.84
39_S66_I0.5420.83
64_G112_D0.5380.83
137_Y146_D0.5360.83
84_F120_K0.5360.83
129_N135_P0.5360.83
30_A34_E0.5320.82
69_Y83_G0.5320.82
28_L45_L0.5290.82
34_E91_A0.5260.82
17_A29_S0.5240.81
10_E27_E0.5210.81
62_S71_D0.5210.81
8_L39_S0.5170.81
39_S42_L0.5140.80
41_A104_D0.5130.80
133_Q137_Y0.5090.80
16_D19_P0.5080.79
7_Y13_M0.5070.79
6_V10_E0.5060.79
23_E30_A0.5050.79
84_F96_P0.5030.79
11_N27_E0.5020.79
110_D113_E0.5000.78
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2dakA 1 0.3399 43 0.904 Contact Map
2jujA 1 0.366 42.1 0.905 Contact Map
4un2B 1 0.2026 37.3 0.907 Contact Map
4dbgB 1 0.3333 36.2 0.908 Contact Map
1z96A 1 0.1765 35.1 0.909 Contact Map
3qr0A 1 0.9935 34.3 0.909 Contact Map
2dahA 1 0.2745 34.3 0.909 Contact Map
1dv0A 1 0.2222 33.9 0.909 Contact Map
2g3qA 1 0.2092 32 0.911 Contact Map
2ragA 2 0.3922 31.9 0.911 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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