GREMLIN Database
DUF475 - Protein of unknown function (DUF475)
PFAM: PF04332 Sequences:
Download Alignment
We filter this alignment to remove sites that have many gaps before running GREMLIN.

Date: 2015_10
Length: 295 (291)
Sequences: 2447 (1531)
Seq/√Len: 89.7
META: 0.743

GREMLIN Results:

Legend: The darker and larger the blue dots, the higher coevolution strength.

Residue pairs sorted by coevolution strength:
i j Sco Prob
64_E67_E4.6061.00
61_D64_E3.9411.00
88_F253_G3.7621.00
19_Q234_E3.7261.00
17_K21_R3.1101.00
29_I33_G2.9721.00
50_G53_E2.9631.00
86_M151_L2.8971.00
21_R25_W2.7681.00
223_M227_S2.6191.00
67_E70_E2.6101.00
99_H109_A2.5711.00
285_F289_I2.5231.00
24_T28_L2.4361.00
51_P54_A2.4181.00
56_T60_S2.3911.00
157_V161_G2.3711.00
261_L266_V2.3591.00
254_A276_I2.3041.00
227_S231_Y2.2681.00
242_R291_H2.2311.00
34_M194_A2.1911.00
78_A143_V2.1821.00
34_M38_F2.1641.00
126_L147_G2.1431.00
94_D97_K2.1241.00
16_P20_R2.1151.00
40_L54_A2.0911.00
53_E56_T2.0361.00
208_T213_L2.0351.00
12_K239_K2.0271.00
179_G182_G1.9511.00
16_P19_Q1.9501.00
119_V123_L1.9161.00
59_L212_P1.9101.00
130_A133_L1.9051.00
82_A122_A1.8961.00
278_V282_G1.8631.00
9_K12_K1.8591.00
120_V123_L1.8141.00
183_L187_L1.8061.00
67_E71_S1.7751.00
36_L215_A1.7561.00
132_W135_L1.7511.00
98_V242_R1.7321.00
75_I142_T1.7301.00
173_K176_G1.7201.00
103_W107_P1.7121.00
143_V263_I1.6951.00
63_P67_E1.6881.00
282_G286_W1.6491.00
112_G115_F1.6411.00
123_L126_L1.6251.00
40_L55_L1.5851.00
254_A280_F1.5841.00
54_A68_I1.5791.00
13_T185_K1.5701.00
130_A134_A1.5571.00
102_G106_R1.5511.00
126_L130_A1.5491.00
20_R24_T1.5481.00
82_A151_L1.5251.00
49_L54_A1.5141.00
257_V261_L1.5121.00
231_Y237_T1.5031.00
27_I223_M1.5021.00
228_L231_Y1.5001.00
286_W290_R1.4811.00
192_L202_I1.4771.00
128_I132_W1.4771.00
88_F92_F1.4701.00
262_S270_E1.4630.99
131_V134_A1.4560.99
78_A263_I1.4540.99
40_L51_P1.4400.99
124_L128_I1.4190.99
158_H162_E1.4180.99
187_L191_V1.4160.99
30_A33_G1.3910.99
129_V133_L1.3830.99
276_I280_F1.3720.99
133_L136_E1.3560.99
114_W117_A1.3530.99
12_K236_G1.3480.99
70_E267_H1.3410.99
27_I227_S1.3260.99
216_L220_I1.3160.99
17_K25_W1.3140.99
123_L148_V1.3060.99
127_L130_A1.3050.99
90_H158_H1.2890.99
66_A69_L1.2880.99
127_L131_V1.2870.99
115_F118_E1.2680.99
107_P111_L1.2670.99
290_R294_R1.2630.98
56_T59_L1.2560.98
41_L45_I1.2550.98
75_I146_A1.2530.98
23_L230_I1.2400.98
120_V124_L1.2360.98
74_P143_V1.2350.98
135_L138_D1.2350.98
104_I107_P1.2280.98
289_I293_R1.2160.98
128_I131_V1.2120.98
52_I56_T1.2120.98
28_L33_G1.1960.98
82_A260_L1.1680.97
107_P110_R1.1670.97
78_A260_L1.1640.97
122_A126_L1.1620.97
56_T61_D1.1590.97
119_V155_F1.1530.97
41_L44_A1.1530.97
73_H262_S1.1500.97
73_H77_A1.1280.97
92_F97_K1.1190.97
228_L232_L1.1170.97
6_V192_L1.1070.96
60_S63_P1.1060.96
47_A57_L1.1060.96
164_L184_S1.0980.96
64_E68_I1.0910.96
125_L132_W1.0910.96
243_Y291_H1.0850.96
231_Y235_K1.0820.96
40_L44_A1.0730.96
117_A120_V1.0640.96
29_I190_E1.0630.96
261_L268_I1.0620.96
274_G277_G1.0570.95
162_E165_E1.0570.95
132_W136_E1.0540.95
87_V202_I1.0540.95
179_G183_L1.0500.95
190_E198_F1.0420.95
47_A68_I1.0420.95
162_E166_K1.0410.95
85_L122_A1.0400.95
66_A209_N1.0390.95
143_V249_H1.0300.95
113_K117_A1.0260.95
106_R110_R1.0260.95
25_W28_L1.0220.95
5_V254_A1.0210.94
79_F153_T1.0190.94
49_L57_L1.0140.94
99_H102_G1.0060.94
27_I190_E1.0060.94
10_V192_L0.9950.94
211_V219_G0.9950.94
120_V156_I0.9920.94
5_V281_I0.9920.94
66_A70_E0.9800.93
175_A178_V0.9690.93
144_L148_V0.9680.93
120_V155_F0.9650.93
151_L155_F0.9550.92
76_I202_I0.9540.92
258_I268_I0.9530.92
161_G195_S0.9500.92
156_I159_G0.9490.92
215_A219_G0.9470.92
126_L132_W0.9370.92
15_S178_V0.9350.91
127_L134_A0.9340.91
271_V275_L0.9340.91
5_V9_K0.9290.91
148_V151_L0.9220.91
258_I276_I0.9210.91
18_W21_R0.9210.91
6_V196_F0.9170.91
280_F286_W0.9140.91
27_I32_F0.9140.91
267_H270_E0.9140.91
78_A82_A0.9070.90
29_I194_A0.8950.90
177_A182_G0.8910.89
75_I216_L0.8850.89
230_I234_E0.8770.89
171_E175_A0.8750.89
29_I186_F0.8730.88
128_I134_A0.8710.88
165_E168_E0.8680.88
61_D66_A0.8680.88
4_A7_N0.8650.88
142_T146_A0.8630.88
77_A262_S0.8550.87
87_V192_L0.8530.87
94_D99_H0.8520.87
187_L190_E0.8440.87
119_V122_A0.8440.87
129_V132_W0.8410.86
208_T225_V0.8390.86
125_L128_I0.8390.86
242_R292_N0.8360.86
17_K20_R0.8350.86
181_S185_K0.8340.86
243_Y287_S0.8310.86
33_G38_F0.8300.86
90_H154_F0.8260.85
85_L89_L0.8240.85
232_L251_A0.8190.85
148_V152_V0.8170.85
131_V135_L0.8160.85
88_F208_T0.8120.84
77_A91_F0.8100.84
32_F35_R0.8090.84
54_A187_L0.8040.84
132_W138_D0.7990.84
268_I278_V0.7990.84
73_H267_H0.7860.83
147_G262_S0.7850.82
10_V188_Y0.7830.82
118_E122_A0.7790.82
34_M198_F0.7790.82
54_A69_L0.7790.82
5_V15_S0.7770.82
82_A126_L0.7760.82
212_P219_G0.7750.82
290_R293_R0.7740.82
99_H103_W0.7730.81
86_M148_V0.7700.81
176_G179_G0.7690.81
261_L272_V0.7620.81
63_P66_A0.7580.80
72_A75_I0.7580.80
286_W289_I0.7560.80
126_L129_V0.7550.80
190_E194_A0.7520.80
90_H155_F0.7470.79
289_I292_N0.7440.79
77_A259_M0.7420.79
145_F152_V0.7420.79
129_V134_A0.7420.79
14_M32_F0.7370.78
61_D65_Y0.7370.78
3_N7_N0.7320.78
90_H115_F0.7320.78
97_K259_M0.7290.78
241_Y285_F0.7260.77
176_G180_K0.7250.77
116_A119_V0.7240.77
93_F159_G0.7230.77
46_A202_I0.7220.77
174_A177_A0.7190.77
127_L149_L0.7190.77
284_A287_S0.7180.76
207_I216_L0.7130.76
154_F158_H0.7120.76
285_F292_N0.7100.76
196_F201_V0.7090.76
63_P209_N0.7090.76
122_A128_I0.7080.75
44_A51_P0.7050.75
268_I284_A0.7000.75
181_S184_S0.7000.75
98_V291_H0.6980.74
266_V272_V0.6980.74
268_I272_V0.6950.74
232_L237_T0.6920.74
7_N11_L0.6910.74
46_A72_A0.6910.74
241_Y289_I0.6900.74
17_K210_N0.6860.73
24_T33_G0.6840.73
130_A135_L0.6840.73
251_A277_G0.6840.73
85_L260_L0.6820.73
178_V182_G0.6800.72
88_F241_Y0.6770.72
91_F158_H0.6770.72
16_P176_G0.6760.72
91_F97_K0.6740.72
247_G284_A0.6730.72
47_A72_A0.6710.72
50_G54_A0.6700.71
240_E292_N0.6680.71
91_F256_A0.6640.71
128_I133_L0.6640.71
18_W186_F0.6630.71
45_I225_V0.6600.70
211_V250_Y0.6590.70
5_V251_A0.6580.70
191_V202_I0.6540.70
125_L129_V0.6530.69
258_I273_T0.6500.69
126_L134_A0.6490.69
120_V159_G0.6470.69
15_S183_L0.6460.69
152_V155_F0.6450.69
23_L28_L0.6450.69
32_F225_V0.6440.68
94_D112_G0.6430.68
59_L211_V0.6410.68
119_V124_L0.6410.68
79_F218_L0.6410.68
172_E175_A0.6390.68
101_L283_L0.6360.67
37_V55_L0.6330.67
79_F84_L0.6330.67
42_I91_F0.6310.67
155_F160_L0.6300.67
237_T240_E0.6300.67
20_R25_W0.6290.67
21_R186_F0.6290.67
272_V276_I0.6270.66
157_V236_G0.6250.66
24_T227_S0.6240.66
90_H118_E0.6210.66
37_V45_I0.6210.66
14_M185_K0.6200.65
285_F290_R0.6190.65
190_E250_Y0.6190.65
167_E174_A0.6180.65
132_W137_A0.6160.65
53_E57_L0.6140.65
69_L167_E0.6130.65
33_G37_V0.6130.65
55_L212_P0.6120.64
129_V257_V0.6120.64
106_R270_E0.6110.64
103_W154_F0.6110.64
230_I245_E0.6100.64
133_L137_A0.6100.64
230_I236_G0.6090.64
29_I191_V0.6070.64
5_V47_A0.6050.64
49_L56_T0.6040.63
4_A11_L0.6000.63
122_A129_V0.6000.63
121_V125_L0.5990.63
44_A49_L0.5980.63
122_A125_L0.5980.63
93_F97_K0.5950.62
2_D7_N0.5940.62
28_L208_T0.5940.62
123_L128_I0.5930.62
120_V126_L0.5930.62
43_V47_A0.5930.62
24_T29_I0.5930.62
6_V13_T0.5920.62
54_A211_V0.5900.62
211_V225_V0.5900.62
124_L156_I0.5880.61
208_T211_V0.5870.61
92_F108_L0.5850.61
101_L104_I0.5830.61
162_E174_A0.5820.61
58_A79_F0.5810.60
198_F223_M0.5810.60
16_P48_G0.5810.60
34_M215_A0.5810.60
79_F198_F0.5800.60
182_G185_K0.5800.60
126_L154_F0.5800.60
154_F159_G0.5780.60
134_A138_D0.5770.60
69_L208_T0.5770.60
120_V125_L0.5760.60
249_H263_I0.5760.60
87_V151_L0.5740.60
16_P152_V0.5730.59
38_F73_H0.5730.59
10_V85_L0.5720.59
101_L112_G0.5710.59
118_E154_F0.5710.59
275_L278_V0.5710.59
3_N11_L0.5690.59
64_E294_R0.5680.59
141_P219_G0.5680.59
210_N216_L0.5660.58
215_A271_V0.5630.58
8_A233_V0.5630.58
69_L211_V0.5620.58
123_L127_L0.5610.58
124_L152_V0.5560.57
15_S182_G0.5560.57
252_I255_L0.5550.57
13_T177_A0.5550.57
65_Y68_I0.5540.57
67_E250_Y0.5540.57
62_P66_A0.5530.57
134_A137_A0.5520.57
98_V102_G0.5500.56
110_R209_N0.5500.56
184_S187_L0.5500.56
79_F186_F0.5490.56
68_I71_S0.5490.56
90_H116_A0.5480.56
163_L167_E0.5480.56
2_D6_V0.5480.56
227_S230_I0.5430.55
9_K239_K0.5420.55
274_G284_A0.5410.55
10_V207_I0.5390.55
140_R156_I0.5380.55
35_R39_P0.5380.55
121_V128_I0.5380.55
78_A134_A0.5370.55
114_W159_G0.5330.54
236_G240_E0.5330.54
105_E112_G0.5320.54
14_M182_G0.5310.54
164_L181_S0.5310.54
90_H159_G0.5300.54
224_F227_S0.5270.53
149_L152_V0.5260.53
268_I273_T0.5240.53
145_F149_L0.5220.53
153_T186_F0.5220.53
76_I91_F0.5210.52
32_F200_G0.5210.52
275_L282_G0.5210.52
86_M207_I0.5200.52
15_S18_W0.5200.52
213_L251_A0.5160.52
236_G241_Y0.5160.52
146_A149_L0.5150.52
177_A180_K0.5140.51
80_G202_I0.5140.51
22_F26_G0.5130.51
103_W112_G0.5120.51
177_A185_K0.5090.51
77_A250_Y0.5080.51
174_A178_V0.5080.51
144_L154_F0.5070.50
51_P195_S0.5040.50
118_E207_I0.5030.50
20_R28_L0.5020.50
82_A143_V0.5010.50
27_I58_A0.5010.50
3_N8_A0.5010.50
232_L239_K0.5000.50
44_A63_P0.5000.50
Legend: The i and j are positions as given in the UniProt sequence. Only predictions with Prob ≥ 0.7 are listed. For all 3L/2 predictions, click on one of the links below.

Sco = ((raw_sco - min_sco)/(avg_sco - min_sco))/2 + 0.5
Prob = P(contact | Sco, seq80/√len)
seq80 = effective number of sequences, after reducing redundancy to 80% identity

HHsearch Results:
Legend: GREMLIN results (Prob ≥ 0.7) overlaid on top PDB hits. The grey/red filled circles underneath are pdb residue contacts (min distance < 5 Angstroms). The shade of the circles is based on the top 10 (or top 20 if e-value < 1E-20) HHsearch results which uses the overall probability and per-site alignment prob. Inter oligomeric contacts in the pdb are in shades of red.
PDB Chains Cov % HHΔ  
2wswA 3 0.2475 9.9 0.977 Contact Map
3udcA 3 0.3119 6.8 0.979 Contact Map
4hw9A 3 0.3051 6.4 0.979 Contact Map
4czbA 2 0.8678 5.7 0.979 Contact Map
2fiuA 2 0.3186 5.4 0.98 Contact Map
2m20A 2 0.0983 5 0.98 Contact Map
4xk8I 1 0.0983 5 0.98 Contact Map
2l9uA 2 0.1051 4.4 0.98 Contact Map
3orgA 2 0.7525 3.7 0.981 Contact Map
5ajiA 3 0.3254 3.6 0.981 Contact Map

Sequence Conservation (Image Generated using WebLogo v3)
Legend: Color is used to show Hydrophobicity (Blue: Hydrophilic, Green: Neutral and Black: Hydrophobic). Amount of gaps is represented by width.

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